MD09G1033600.v1.1

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001188
Physical Location & Seq
Reverse (-)
2059256 .. 2061179
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1033600.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGCGGCTGCTGTAGCTTCAAGTGATTCAGTTTCCATACCATCTGTAAACAAGGCGTGGGTGTACTCAGAGCACGGAAAGTCTGCAGATGTTTTGAAGTTCGATCCGAATGTTCCTGTTCCTGAAATAAAGGAAGACCAGGTGCTGATCAAGGTGGTTGCTGCATCTCTCAACCCAGTTGATTTCAAAAGGATGCTTGGCTACTTCAAGGACTCTGACTCTTCACCCCCTACGGTTCCAGGGTATGATGTTGCTGGTGCGGTGGTGAAAGTAGGAAGCCGGGTGACAAAGTTTAAGGTGGGGGATGAAGTATATGGGGATCTCAACGAGAAGTCTCTCGATAAGCCGAAAAAGATCGGATCATTGGCCGAGTACACTGCTGCAGAAGAAAGAGTGTTAGCTCTCAAGCCAAAAAATCTGAGTTTTGTTGAAGCTGCTAGCCTTCCCCTGGCCATTGAGACTGCCTATGAAGGGCTCGAACGAGTTGAATTCTCTGCCGGTAAATCCATCCTTGTTTTGGGAGGCGCTGGGGGAGTCGGAACACATGTTATTCAGCTAGCAAAGCATGTGTTTAGTGCTTCAAAAGTAGCAGCTACAGCGAGCACGAAAAAACTCGATCTTTTGAGAAGCTTGGGTGCTGATTTGGCTATTGATTACACCAAGGAGAACTTCGAAGACCTGCCTGAGAAATTCGATGTGGTTTATGATGCAGTTGGGCAGAGTGACAGGGCATTGAAGGCGGTGAAGGAAGGCGGGAAGGTTGTGACAATCATAGGTCCAGTAACGCCACCGGCAATCATATTTGTGCTTACTTCAACCGGAGCTGTATTAGAGAAACTGAAGCCTTACTTGGAGAGTGGGAAGGTGAAGCCGGTGCTTGATCCCACCGGCCCGTATCCATTTTCGAAGACTGTTGAAGCATTTGCCTATCTTGAAACCTCCAGAGCTACCGGAAAGGTGGTTGTGTATCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

327

Amino Acids

34.64

Weight (kDa)

6.37

Isoelectric Point (pI)

22.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 45 - 137 2.8e-16 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 178 - 257 7.2e-13 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 211 - 322 2.3e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 687
AciI CCGC 4 cut(s) 5, 260, 740, 753
AclWI GGATC 4 cut(s) 98, 327, 367, 875
AcoI YGGCCR 2 cut(s) 366, 450
AcsI RAATTY 2 cut(s) 488, 689
AcuI CTGAAG 1 cut(s) 860
AfaI GTAC 2 cut(s) 65, 374
AfiI CCNNNNNNNGG 3 cut(s) 448, 471, 517
AflIII ACRYGT 1 cut(s) 544
AjnI CCWGG 3 cut(s) 138, 238, 447
AjuI GAANNNNNNNTTGG 4 cut(s) 653, 685, 833, 865
AluBI AGCT 8 cut(s) 17, 401, 434, 556, 593, 630, 824, 947
AluI AGCT 8 cut(s) 17, 401, 434, 556, 593, 630, 824, 947
Alw21I GWGCWC 2 cut(s) 75, 605
Alw26I GTCTC 2 cut(s) 339, 452
AlwI GGATC 4 cut(s) 98, 327, 367, 875
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 3 cut(s) 366, 450, 889
ApeKI GCWGC 5 cut(s) 8, 161, 380, 434, 590
ApoI RAATTY 2 cut(s) 488, 689
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 2 cut(s) 776, 890
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 5 cut(s) 216, 277, 295, 754, 877
AsuII TTCGAA 2 cut(s) 672, 905
AsuNHI GCTAGC 2 cut(s) 437, 556
AvaII GGWCC 1 cut(s) 776
BalI TGGCCA 1 cut(s) 452
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 3 cut(s) 141, 681, 914
Bbv12I GWGCWC 2 cut(s) 75, 605
BbvI GCAGC 4 cut(s) 148, 367, 421, 602
BccI CCATC 3 cut(s) 49, 515, 980
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 3 cut(s) 140, 240, 449
BciVI GTATCC 2 cut(s) 906, 978
BclI TGATCA 1 cut(s) 147
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 339, 452
BfaI CTAG 2 cut(s) 438, 557
BfmI CTRYAG 4 cut(s) 12, 84, 381, 594
BfoI RGCGCY 1 cut(s) 528
BfuAI ACCTGC 1 cut(s) 687
BfuI GTATCC 2 cut(s) 906, 978
BisI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
BlsI GCNGC 6 cut(s) 7, 10, 163, 382, 436, 592
Bme1390I CCNGG 4 cut(s) 140, 240, 281, 449
Bme18I GGWCC 1 cut(s) 776
BmgT120I GGNCC 2 cut(s) 776, 890
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 4 cut(s) 140, 240, 281, 449
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 3 cut(s) 173, 183, 697
BmtI GCTAGC 2 cut(s) 441, 560
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 3 cut(s) 141, 681, 914
BpmI CTGGAG 1 cut(s) 925
Bpu14I TTCGAA 2 cut(s) 672, 905
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 281
BsaJI CCNNGG 3 cut(s) 239, 447, 660
BsaWI WCCGGW 2 cut(s) 818, 950
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 471, 517
Bse118I RCCGGY 4 cut(s) 497, 790, 871, 887
Bse1I ACTGG 2 cut(s) 176, 779
BseBI CCWGG 3 cut(s) 140, 240, 449
BseDI CCNNGG 3 cut(s) 239, 447, 660
BseGI GGATG 4 cut(s) 198, 310, 507, 972
BseLI CCNNNNNNNGG 3 cut(s) 448, 471, 517
BseMII CTCAG 3 cut(s) 81, 410, 675
BseNI ACTGG 2 cut(s) 176, 779
BseXI GCAGC 4 cut(s) 148, 367, 421, 602
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 3 cut(s) 368, 452, 891
BsiHKAI GWGCWC 2 cut(s) 75, 605
BsiSI CCGG 7 cut(s) 280, 498, 791, 819, 872, 888, 951
BslI CCNNNNNNNGG 3 cut(s) 448, 471, 517
BsmAI GTCTC 2 cut(s) 339, 452
BsnI GGCC 3 cut(s) 368, 452, 891
Bsp119I TTCGAA 2 cut(s) 672, 905
Bsp1286I GDGCHC 3 cut(s) 75, 477, 605
Bsp143I GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
BspACI CCGC 4 cut(s) 5, 260, 740, 753
BspANI GGCC 3 cut(s) 368, 452, 891
BspCNI CTCAG 3 cut(s) 80, 411, 676
BspLI GGNNCC 1 cut(s) 237
BspMAI CTGCAG 2 cut(s) 88, 385
BspMI ACCTGC 1 cut(s) 687
BspOI GCTAGC 2 cut(s) 441, 560
BspPI GGATC 4 cut(s) 98, 327, 367, 875
BspT104I TTCGAA 2 cut(s) 672, 905
BsrFI RCCGGY 4 cut(s) 497, 790, 871, 887
BsrI ACTGG 2 cut(s) 176, 779
BssAI RCCGGY 4 cut(s) 497, 790, 871, 887
BssECI CCNNGG 3 cut(s) 239, 447, 660
BssMI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 3 cut(s) 140, 240, 449
Bst4CI ACNGT 2 cut(s) 235, 913
Bst6I CTCTTC 1 cut(s) 226
BstBI TTCGAA 2 cut(s) 672, 905
BstC8I GCNNGC 3 cut(s) 439, 558, 601
BstDEI CTNAG 3 cut(s) 67, 419, 684
BstF5I GGATG 4 cut(s) 198, 310, 507, 972
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 7 cut(s) 106, 150, 322, 357, 362, 619, 883
BstMAI GTCTC 2 cut(s) 339, 452
BstMBI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
BstMWI GCNNNNNNNGC 5 cut(s) 14, 562, 596, 644, 737
BstNI CCWGG 3 cut(s) 140, 240, 449
BstNSI RCATGY 2 cut(s) 548, 569
BstSCI CCNGG 4 cut(s) 138, 238, 279, 447
BstSFI CTRYAG 4 cut(s) 12, 84, 381, 594
BstV1I GCAGC 4 cut(s) 148, 367, 421, 602
BstV2I GAAGAC 3 cut(s) 141, 681, 914
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BsuI GTATCC 2 cut(s) 906, 978
BsuRI GGCC 3 cut(s) 368, 452, 891
BtsCI GGATG 4 cut(s) 198, 310, 507, 972
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 375
BveI ACCTGC 1 cut(s) 687
Cac8I GCNNGC 3 cut(s) 439, 558, 601
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 1 cut(s) 527
Cfr10I RCCGGY 4 cut(s) 497, 790, 871, 887
Cfr13I GGNCC 2 cut(s) 776, 890
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 2 cut(s) 64, 373
CviAII CATG 3 cut(s) 545, 566, 978
CviQI GTAC 2 cut(s) 64, 373
DdeI CTNAG 3 cut(s) 67, 419, 684
DpnI GATC 7 cut(s) 105, 149, 321, 356, 361, 618, 882
DpnII GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
EaeI YGGCCR 2 cut(s) 366, 450
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 660
Eco24I GRGCYC 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 776
Eco57I CTGAAG 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 3 cut(s) 138, 238, 447
EcoT14I CCWWGG 1 cut(s) 660
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 3 cut(s) 548, 569, 981
FalI AAGNNNNNCTT 4 cut(s) 653, 685, 833, 865
FatI CATG 3 cut(s) 544, 565, 977
FauI CCCGC 1 cut(s) 746
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
FokI GGATG 4 cut(s) 205, 317, 494, 959
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
FspBI CTAG 2 cut(s) 438, 557
GlaI GCGC 1 cut(s) 526
GluI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 925
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 3 cut(s) 368, 452, 891
HapII CCGG 7 cut(s) 280, 498, 791, 819, 872, 888, 951
HhaI GCGC 1 cut(s) 527
Hin1II CATG 3 cut(s) 548, 569, 981
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 4 cut(s) 26, 212, 218, 534
HpaII CCGG 7 cut(s) 280, 498, 791, 819, 872, 888, 951
HphI GGTGA 5 cut(s) 216, 277, 295, 754, 877
Hpy166II GTNNAC 3 cut(s) 49, 64, 375
Hpy188I TCNGA 6 cut(s) 70, 108, 217, 359, 420, 539
Hpy188III TCNNGA 4 cut(s) 122, 338, 932, 942
Hpy8I GTNNAC 3 cut(s) 49, 64, 375
HpyAV CCTTC 7 cut(s) 452, 464, 730, 739, 743, 751, 856
HpyCH4III ACNGT 2 cut(s) 235, 913
HpyCH4V TGCA 4 cut(s) 86, 164, 383, 710
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 562, 596, 644, 737
HpyF3I CTNAG 3 cut(s) 67, 419, 684
Hsp92II CATG 3 cut(s) 548, 569, 981
HspAI GCGC 1 cut(s) 525
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
LmnI GCTCC 1 cut(s) 821
Lsp1109I GCAGC 4 cut(s) 148, 367, 421, 602
LweI GCATC 3 cut(s) 173, 183, 697
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 2 cut(s) 438, 557
MaeIII GTNAC 4 cut(s) 283, 722, 763, 781
MalI GATC 7 cut(s) 105, 149, 321, 356, 361, 618, 882
MboI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
MboII GAAGA 5 cut(s) 146, 213, 398, 686, 919
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 3 cut(s) 75, 477, 605
MlsI TGGCCA 1 cut(s) 452
MluCI AATT 2 cut(s) 488, 689
MluNI TGGCCA 1 cut(s) 452
MlyI GAGTC 3 cut(s) 206, 212, 543
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 2 cut(s) 515, 949
Mox20I TGGCCA 1 cut(s) 452
MscI TGGCCA 1 cut(s) 452
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 803
Msp20I TGGCCA 1 cut(s) 452
MspI CCGG 7 cut(s) 280, 498, 791, 819, 872, 888, 951
MspR9I CCNGG 4 cut(s) 140, 240, 281, 449
MvaI CCWGG 3 cut(s) 140, 240, 449
MwoI GCNNNNNNNGC 5 cut(s) 14, 562, 596, 644, 737
NciI CCSGG 1 cut(s) 281
NdeII GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
NheI GCTAGC 2 cut(s) 437, 556
NlaIII CATG 3 cut(s) 548, 569, 981
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 3 cut(s) 283, 722, 763
NspI RCATGY 2 cut(s) 548, 569
NspV TTCGAA 2 cut(s) 672, 905
PciI ACATGT 1 cut(s) 544
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 6 cut(s) 7, 10, 163, 382, 436, 592
PleI GAGTC 3 cut(s) 206, 212, 542
PpsI GAGTC 3 cut(s) 206, 212, 542
PscI ACATGT 1 cut(s) 544
Psp6I CCWGG 3 cut(s) 138, 238, 447
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 3 cut(s) 138, 238, 447
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 2 cut(s) 776, 890
PstI CTGCAG 2 cut(s) 88, 385
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 319
RsaI GTAC 2 cut(s) 65, 374
RsaNI GTAC 2 cut(s) 64, 373
RseI CAYNNNNRTG 1 cut(s) 803
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
Sau3AI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
Sau96I GGNCC 2 cut(s) 776, 890
SchI GAGTC 3 cut(s) 206, 212, 543
ScrFI CCNGG 4 cut(s) 140, 240, 281, 449
SduI GDGCHC 3 cut(s) 75, 477, 605
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 3 cut(s) 173, 183, 697
SfcI CTRYAG 4 cut(s) 12, 84, 381, 594
SfuI TTCGAA 2 cut(s) 672, 905
SinI GGWCC 1 cut(s) 776
SmiMI CAYNNNNRTG 1 cut(s) 803
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 2 cut(s) 488, 689
SsiI CCGC 4 cut(s) 5, 260, 740, 753
SspMI CTAG 2 cut(s) 438, 557
StyD4I CCNGG 4 cut(s) 138, 238, 279, 447
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 2 cut(s) 235, 913
TaqI TCGA 7 cut(s) 102, 339, 477, 615, 672, 693, 905
TasI AATT 2 cut(s) 488, 689
TatI WGTACW 2 cut(s) 63, 372
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 3 cut(s) 283, 722, 763
TseI GCWGC 5 cut(s) 8, 161, 380, 434, 590
Tsp45I GTSAC 3 cut(s) 283, 722, 763
TspDTI ATGAA 2 cut(s) 321, 483
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 776
XapI RAATTY 2 cut(s) 488, 689
XceI RCATGY 2 cut(s) 548, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XspI CTAG 2 cut(s) 438, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.