RLG00000023107

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001188
Physical Location & Seq
Forward (+)
18174041 .. 18177787
3747 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023107

Sequence Viewer

Length: 936 bp
ATGGCTGCACCTCCAAGTGATTCCATAACCTCTGTAATGGAGGCTTGGGTCTATTCAGAGTATGGAAAAACTTCTGATGTTCTAAAGCTTGATCCAAGTGTGGCTGTTCCTGAAATTAAAGAGGATCAGGTGTTGATCAAGGTGGTTGCTGCCTCTCTTAACCCAATTGATTTCAAAAGGGCTCTTGGTTACTTCAAGGACACTGACTCTCCCCTACCTACAGTTCCAGGGTATGATGTGGCTGGTGTGGTGTTTAAGGTGGGGGATGAAGTATATGGGGATCTCAATGAGACAGTGAACCCAAAAAGGTTTGGGTCTTTGGCACAGTACACTGCTGCAGAAGAAAGAATATTGGCTCACAAACCCAAAAACCTGAGCTTTATCGAAGCTGCTAGCCTTCCCTTAGCCATTGAGACTGCCTATGAAGGGCTTGGAAGAGCCGAACTTTCTGCTGGTAAATCCATTCTTGTTTTGGGAGGCGCTGGGGGTGTGGGAACACATATTATTCAGCTGGCAAAGCATGTTTTTGGTGCTTCCAAAGTGGCAGCTACTGCAAGCACTAAGAAACTGGATTTGTTGAGAAGCTTGGGTGCTGATTTGCCTATCGATTACACCAAGGTGAACATCGAAGACCTGCCAGAGAAATTTGATGTAGTGTATGACGCAGTTGGGGAGACTGACAAGGCTGTAAAGGCGGTGAAAGAAGGCGGGAAGGTAGTAACAATAGTAGGTCCAGCAACGCCACCAGCTATCATATTTCTGCTTACCACTAAAGGGTCTATCTTGGAGAAACTGAAGCCCTACTTGGAGGGTGGGAAAGTGAAGCCAGTGCTTGATCCCACAGGTCCATATCCCTTTTCTAAAGTTGTGGAAGCATTTGCTTACCTTGAGACTTCCAGAGTTACAGGAAAGGTAGTTGTGCATCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

312

Amino Acids

33.03

Weight (kDa)

5.71

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 92 1.1e-08 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 136 - 235 9.9e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 163 - 248 9e-13 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 196 - 307 2e-18 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 642
AciI CCGC 2 cut(s) 695, 708
AclWI GGATC 4 cut(s) 86, 132, 288, 830
AcsI RAATTY 1 cut(s) 644
AcuI CTGAAG 1 cut(s) 815
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 2 cut(s) 426, 774
AgsI TTSAA 2 cut(s) 175, 196
AjnI CCWGG 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 7 cut(s) 88, 378, 389, 511, 548, 585, 749
AluI AGCT 7 cut(s) 88, 378, 389, 511, 548, 585, 749
Alw26I GTCTC 4 cut(s) 284, 407, 668, 884
AlwI GGATC 4 cut(s) 86, 132, 288, 830
AlwNI CAGNNNCTG 1 cut(s) 551
ApeKI GCWGC 5 cut(s) 5, 149, 335, 389, 545
ApoI RAATTY 1 cut(s) 644
Asp700I GAANNNNTTC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 2 cut(s) 731, 845
AsuHPI GGTGA 2 cut(s) 631, 709
AsuNHI GCTAGC 1 cut(s) 392
AvaII GGWCC 2 cut(s) 731, 845
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 636
BbvI GCAGC 4 cut(s) 136, 322, 376, 557
BccI CCATC 1 cut(s) 935
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 4 cut(s) 284, 407, 668, 884
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 2 cut(s) 219, 336
BfoI RGCGCY 1 cut(s) 483
BfuAI ACCTGC 1 cut(s) 642
BisI GCNGC 5 cut(s) 6, 150, 336, 390, 546
BlsI GCNGC 5 cut(s) 7, 151, 337, 391, 547
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 2 cut(s) 731, 845
BmgT120I GGNCC 2 cut(s) 731, 845
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 931
BmtI GCTAGC 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 636
Bpu10I CCTNAGC 2 cut(s) 374, 403
BpuEI CTTGAG 1 cut(s) 908
Bsa29I ATCGAT 1 cut(s) 606
BsaJI CCNNGG 2 cut(s) 227, 615
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 774
Bse1I ACTGG 2 cut(s) 573, 827
BseBI CCWGG 1 cut(s) 228
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 227, 615
BseGI GGATG 3 cut(s) 271, 922, 927
BseLI CCNNNNNNNGG 2 cut(s) 426, 774
BseMII CTCAG 1 cut(s) 365
BseNI ACTGG 2 cut(s) 573, 827
BseXI GCAGC 4 cut(s) 136, 322, 376, 557
BseYI CCCAGC 1 cut(s) 482
BshVI ATCGAT 1 cut(s) 606
BslI CCNNNNNNNGG 2 cut(s) 426, 774
BsmAI GTCTC 4 cut(s) 284, 407, 668, 884
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 5 cut(s) 91, 124, 135, 280, 835
BspACI CCGC 2 cut(s) 695, 708
BspCNI CTCAG 1 cut(s) 366
BspDI ATCGAT 1 cut(s) 606
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 642
BspOI GCTAGC 1 cut(s) 396
BspPI GGATC 4 cut(s) 86, 132, 288, 830
BspQI GCTCTTC 1 cut(s) 430
BsrI ACTGG 2 cut(s) 573, 827
BssECI CCNNGG 2 cut(s) 227, 615
BssMI GATC 5 cut(s) 91, 124, 135, 280, 835
BssT1I CCWWGG 1 cut(s) 615
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 3 cut(s) 223, 295, 327
Bst6I CTCTTC 1 cut(s) 430
BstAPI GCANNNNNTGC 1 cut(s) 551
BstC8I GCNNGC 3 cut(s) 394, 513, 556
BstDEI CTNAG 3 cut(s) 374, 403, 561
BstF5I GGATG 3 cut(s) 271, 922, 927
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 5 cut(s) 94, 127, 138, 283, 838
BstMAI GTCTC 4 cut(s) 284, 407, 668, 884
BstMBI GATC 5 cut(s) 91, 124, 135, 280, 835
BstMWI GCNNNNNNNGC 3 cut(s) 517, 551, 692
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 2 cut(s) 219, 336
BstV1I GCAGC 4 cut(s) 136, 322, 376, 557
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
Bsu15I ATCGAT 1 cut(s) 606
BsuTUI ATCGAT 1 cut(s) 606
BtsCI GGATG 3 cut(s) 271, 922, 927
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 4 cut(s) 201, 300, 330, 834
BveI ACCTGC 1 cut(s) 642
Cac8I GCNNGC 3 cut(s) 394, 513, 556
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 1 cut(s) 482
Cfr13I GGNCC 2 cut(s) 731, 845
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 1 cut(s) 671
Csp6I GTAC 1 cut(s) 328
CviAII CATG 2 cut(s) 521, 933
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 3 cut(s) 374, 403, 561
DpnI GATC 5 cut(s) 93, 126, 137, 282, 837
DpnII GATC 5 cut(s) 91, 124, 135, 280, 835
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 615
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 731, 845
Eco57I CTGAAG 1 cut(s) 815
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 615
FaeI CATG 2 cut(s) 524, 936
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FatI CATG 2 cut(s) 520, 932
FauI CCCGC 1 cut(s) 701
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 6, 150, 336, 390, 546
FokI GGATG 3 cut(s) 278, 909, 914
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 5 cut(s) 6, 150, 336, 390, 546
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 481
GluI GCNGC 5 cut(s) 6, 150, 336, 390, 546
GsaI CCCAGC 1 cut(s) 486
HaeII RGCGCY 1 cut(s) 483
HgaI GACGC 1 cut(s) 671
HhaI GCGC 1 cut(s) 482
Hin1II CATG 2 cut(s) 524, 936
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HindIII AAGCTT 2 cut(s) 86, 583
HinfI GANTC 2 cut(s) 20, 206
HphI GGTGA 2 cut(s) 631, 709
Hpy166II GTNNAC 3 cut(s) 298, 330, 622
Hpy188I TCNGA 2 cut(s) 58, 76
Hpy188III TCNNGA 2 cut(s) 110, 897
Hpy8I GTNNAC 3 cut(s) 298, 330, 622
HpyAV CCTTC 4 cut(s) 407, 419, 698, 706
HpyCH4III ACNGT 3 cut(s) 223, 295, 327
HpyCH4V TGCA 4 cut(s) 8, 338, 554, 922
HpyF10VI GCNNNNNNNGC 3 cut(s) 517, 551, 692
HpyF3I CTNAG 3 cut(s) 374, 403, 561
Hsp92II CATG 2 cut(s) 524, 936
HspAI GCGC 1 cut(s) 480
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 5 cut(s) 91, 124, 135, 280, 835
LguI GCTCTTC 1 cut(s) 430
Lsp1109I GCAGC 4 cut(s) 136, 322, 376, 557
LweI GCATC 1 cut(s) 931
MaeI CTAG 1 cut(s) 393
MaeIII GTNAC 3 cut(s) 188, 718, 901
MalI GATC 5 cut(s) 93, 126, 137, 282, 837
MboI GATC 5 cut(s) 91, 124, 135, 280, 835
MboII GAAGA 3 cut(s) 353, 447, 641
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 3 cut(s) 114, 165, 644
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 7 cut(s) 21, 34, 40, 115, 163, 470, 802
MroXI GAANNNNTTC 1 cut(s) 70
MseI TTAA 3 cut(s) 117, 159, 255
MslI CAYNNNNRTG 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 511
MspR9I CCNGG 1 cut(s) 228
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 3 cut(s) 517, 551, 692
NdeII GATC 5 cut(s) 91, 124, 135, 280, 835
NheI GCTAGC 1 cut(s) 392
NlaIII CATG 2 cut(s) 524, 936
NspI RCATGY 1 cut(s) 524
OliI CACNNNNGTG 1 cut(s) 617
PciSI GCTCTTC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 5 cut(s) 7, 151, 337, 391, 547
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 482
PspGI CCWGG 1 cut(s) 226
PspPI GGNCC 2 cut(s) 731, 845
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 430
SaqAI TTAA 3 cut(s) 117, 159, 255
SatI GCNGC 5 cut(s) 6, 150, 336, 390, 546
Sau3AI GATC 5 cut(s) 91, 124, 135, 280, 835
Sau96I GGNCC 2 cut(s) 731, 845
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 184
SfaNI GCATC 1 cut(s) 931
SfcI CTRYAG 2 cut(s) 219, 336
SinI GGWCC 2 cut(s) 731, 845
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse9I AATT 3 cut(s) 114, 165, 644
SsiI CCGC 2 cut(s) 695, 708
SspI AATATT 1 cut(s) 351
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 615
TaaI ACNGT 3 cut(s) 223, 295, 327
TaqI TCGA 3 cut(s) 384, 606, 627
TasI AATT 3 cut(s) 114, 165, 644
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 3 cut(s) 117, 159, 255
Tru9I TTAA 3 cut(s) 117, 159, 255
TscAI CASTG 4 cut(s) 208, 300, 337, 834
TseI GCWGC 5 cut(s) 5, 149, 335, 389, 545
TspDTI ATGAA 2 cut(s) 282, 438
TspRI CASTG 4 cut(s) 208, 300, 337, 834
VpaK11BI GGWCC 2 cut(s) 731, 845
XapI RAATTY 1 cut(s) 644
XceI RCATGY 1 cut(s) 524
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.