Rh2BG639600

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001188
Physical Location & Seq
Forward (+)
85917088 .. 85919198
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG639600.1

Sequence Viewer

Length: 1035 bp
ATGGCTGCAGCTCCAAGTGAGACCATACCCTCTGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATGCAAATGTGGCTGTTCCTGAAATTAAAGAGGACCAGATGCTGATCAAGGTGGTTGCTGCTTCTCTTAACCCAATTGATTCCAGAAGGGCTCTTGGTCTCTACAAGGACATAGAGGGTGATACTACCTTACCAACAGTTCCAGGGTATGACGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAAGAATAGCCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAGAGTACACGGCTGCAGGAGAAACCATGTTGGCTCTCAAACCCAAAAACCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCTATTGAGACTGCGTATGGAGCGCTTGAAAGAGCGGAGCTTTCGGCTGGTAAATCCGTCCTTGTTTTGGGAGGCTCTGGGGGTGTTGGAACACATGTGATTCAGCTAGCTAAACATGTTTTTGGGGCATCCAAAGTAGCAGCTACTGCAAGCACTAAAAAACTGGATTTGTTAAGAAGCTTGGGTGCGGATTTGGCTATTGATTACACCAAGGAAAACATCGAAGACCTGCCAGAGAAATTTGATGTAGTCTATGATGCAGTTGTGGGGCAGACTGACAAGGCAGTGAAGGCGGTGAAGGAAGGTGGGAAGGTAGTGACAATAGTCTTAGGTCCAGTAACTCCTCCGGCGTTCAGATTTGTGCTCACCCCTACAGCGTCTATCTTGGAGAAACTGAAGCCTTACTTGGAGAGCGGGAAGGTGAAGCCAGTGCTTGATCCCACAGGTCCATATCCCTTTTCTAAAGTTGTTGAAGCACTTGGTTACCTTGAGACTTCTAGAGCTACAGGAAAGGTGGTTGTGTACCCTATCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

344

Amino Acids

36.43

Weight (kDa)

6.16

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 153 9.7e-16 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 194 - 277 6.6e-14 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 227 - 340 4.1e-19 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 735
AccBSI CCGCTC 2 cut(s) 533, 912
AciI CCGC 4 cut(s) 533, 686, 791, 912
AclWI GGATC 2 cut(s) 375, 929
AcsI RAATTY 2 cut(s) 86, 737
AcuI CTGAAG 1 cut(s) 914
AfaI GTAC 2 cut(s) 422, 1022
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 1 cut(s) 565
AflIII ACRYGT 2 cut(s) 592, 613
AgsI TTSAA 3 cut(s) 479, 527, 971
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 1 cut(s) 232
AjuI GAANNNNNNNTTGG 2 cut(s) 887, 919
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 4 cut(s) 14, 194, 500, 983
AlwI GGATC 2 cut(s) 375, 929
AlwNI CAGNNNCTG 2 cut(s) 133, 644
Aor51HI AGCGCT 1 cut(s) 522
ApeKI GCWGC 6 cut(s) 5, 8, 149, 428, 482, 638
ApoI RAATTY 2 cut(s) 86, 737
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 3 cut(s) 124, 830, 944
AsuHPI GGTGA 6 cut(s) 221, 271, 400, 805, 856, 931
AsuNHI GCTAGC 2 cut(s) 485, 604
AvaII GGWCC 3 cut(s) 124, 830, 944
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 729
Bbv12I GWGCWC 1 cut(s) 864
BbvI GCAGC 5 cut(s) 20, 136, 415, 469, 650
BceAI ACGGC 1 cut(s) 441
BciT130I CCWGG 1 cut(s) 234
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 4 cut(s) 14, 194, 500, 983
BfaI CTAG 3 cut(s) 486, 605, 996
BfmI CTRYAG 5 cut(s) 6, 72, 429, 870, 1002
BfoI RGCGCY 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 735
BisI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
BlsI GCNGC 6 cut(s) 7, 10, 151, 430, 484, 640
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 3 cut(s) 124, 830, 944
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 3 cut(s) 124, 830, 944
BmrFI CCNGG 1 cut(s) 234
BmsI GCATC 4 cut(s) 82, 120, 635, 745
BmtI GCTAGC 2 cut(s) 489, 608
BoxI GACNNNNGTC 1 cut(s) 821
BpiI GAAGAC 1 cut(s) 729
Bpu10I CCTNAGC 2 cut(s) 467, 496
BpuEI CTTGAG 1 cut(s) 1007
BsaBI GATNNNNATC 1 cut(s) 134
BsaI GGTCTC 2 cut(s) 14, 194
BsaJI CCNNGG 3 cut(s) 233, 361, 708
Bsc4I CCNNNNNNNGG 1 cut(s) 565
Bse1I ACTGG 3 cut(s) 666, 833, 926
Bse8I GATNNNNATC 1 cut(s) 134
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 233, 361, 708
BseGI GGATG 2 cut(s) 304, 626
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 1 cut(s) 565
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 3 cut(s) 666, 833, 926
BseRI GAGGAG 1 cut(s) 831
BseXI GCAGC 5 cut(s) 20, 136, 415, 469, 650
BseYI CCCAGC 1 cut(s) 403
BsgI GTGCAG 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 864
BsiSI CCGG 1 cut(s) 845
BslI CCNNNNNNNGG 1 cut(s) 565
BsmAI GTCTC 4 cut(s) 14, 194, 500, 983
Bso31I GGTCTC 2 cut(s) 14, 194
Bsp1286I GDGCHC 2 cut(s) 184, 864
Bsp143I GATC 3 cut(s) 135, 367, 934
Bsp19I CCATGG 1 cut(s) 361
BspACI CCGC 4 cut(s) 533, 686, 791, 912
BspCNI CTCAG 1 cut(s) 459
BspMAI CTGCAG 3 cut(s) 10, 76, 433
BspMI ACCTGC 1 cut(s) 735
BspOI GCTAGC 2 cut(s) 489, 608
BspPI GGATC 2 cut(s) 375, 929
BspTNI GGTCTC 2 cut(s) 14, 194
BsrBI CCGCTC 2 cut(s) 533, 912
BsrI ACTGG 3 cut(s) 666, 833, 926
BssECI CCNNGG 3 cut(s) 233, 361, 708
BssMI GATC 3 cut(s) 135, 367, 934
BssT1I CCWWGG 2 cut(s) 361, 708
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 229
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 3 cut(s) 487, 606, 649
BstDEI CTNAG 4 cut(s) 351, 467, 496, 826
BstDSI CCRYGG 1 cut(s) 361
BstEII GGTNACC 1 cut(s) 980
BstF5I GGATG 2 cut(s) 304, 626
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 3 cut(s) 138, 370, 937
BstMAI GTCTC 4 cut(s) 14, 194, 500, 983
BstMBI GATC 3 cut(s) 135, 367, 934
BstMWI GCNNNNNNNGC 5 cut(s) 101, 518, 644, 692, 788
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 2 cut(s) 596, 617
BstPAI GACNNNNGTC 1 cut(s) 821
BstPI GGTNACC 1 cut(s) 980
BstSCI CCNGG 1 cut(s) 232
BstSFI CTRYAG 5 cut(s) 6, 72, 429, 870, 1002
BstV1I GCAGC 5 cut(s) 20, 136, 415, 469, 650
BstV2I GAAGAC 1 cut(s) 729
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 361
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 2 cut(s) 304, 626
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 789, 933
BveI ACCTGC 1 cut(s) 735
Cac8I GCNNGC 3 cut(s) 487, 606, 649
CaiI CAGNNNCTG 2 cut(s) 133, 644
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 3 cut(s) 124, 830, 944
CseI GACGC 1 cut(s) 864
Csp6I GTAC 2 cut(s) 421, 1021
CviAII CATG 6 cut(s) 347, 362, 442, 593, 614, 1032
CviQI GTAC 2 cut(s) 421, 1021
DdeI CTNAG 4 cut(s) 351, 467, 496, 826
DpnI GATC 3 cut(s) 137, 369, 936
DpnII GATC 3 cut(s) 135, 367, 934
Eco130I CCWWGG 2 cut(s) 361, 708
Eco24I GRGCYC 1 cut(s) 184
Eco31I GGTCTC 2 cut(s) 14, 194
Eco47I GGWCC 3 cut(s) 124, 830, 944
Eco47III AGCGCT 1 cut(s) 522
Eco57I CTGAAG 1 cut(s) 914
Eco91I GGTNACC 1 cut(s) 980
EcoO65I GGTNACC 1 cut(s) 980
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 361, 708
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 361, 708
FaeI CATG 6 cut(s) 350, 365, 445, 596, 617, 1035
FalI AAGNNNNNCTT 2 cut(s) 887, 919
FatI CATG 6 cut(s) 346, 361, 441, 592, 613, 1031
FauI CCCGC 1 cut(s) 905
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
FokI GGATG 2 cut(s) 311, 613
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
FspBI CTAG 3 cut(s) 486, 605, 996
GlaI GCGC 1 cut(s) 522
GluI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
GsaI CCCAGC 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 524
HapII CCGG 1 cut(s) 845
HgaI GACGC 1 cut(s) 864
HhaI GCGC 1 cut(s) 523
Hin1II CATG 6 cut(s) 350, 365, 445, 596, 617, 1035
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 3 cut(s) 170, 334, 598
HpaII CCGG 1 cut(s) 845
HphI GGTGA 6 cut(s) 221, 271, 400, 805, 856, 931
Hpy166II GTNNAC 2 cut(s) 423, 1021
Hpy188I TCNGA 3 cut(s) 58, 84, 854
Hpy188III TCNNGA 3 cut(s) 110, 174, 996
Hpy8I GTNNAC 2 cut(s) 423, 1021
HpyAV CCTTC 8 cut(s) 171, 256, 500, 781, 790, 794, 802, 910
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 7 cut(s) 8, 74, 95, 280, 431, 647, 758
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 518, 644, 692, 788
HpyF3I CTNAG 4 cut(s) 351, 467, 496, 826
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 6 cut(s) 350, 365, 445, 596, 617, 1035
HspAI GCGC 1 cut(s) 521
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 3 cut(s) 135, 367, 934
LmnI GCTCC 3 cut(s) 16, 518, 535
Lsp1109I GCAGC 5 cut(s) 20, 136, 415, 469, 650
LweI GCATC 4 cut(s) 82, 120, 635, 745
MaeI CTAG 3 cut(s) 486, 605, 996
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 3 cut(s) 814, 835, 980
MalI GATC 3 cut(s) 137, 369, 936
MbiI CCGCTC 2 cut(s) 533, 912
MboI GATC 3 cut(s) 135, 367, 934
MboII GAAGA 1 cut(s) 734
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 2 cut(s) 184, 864
MluCI AATT 4 cut(s) 86, 114, 165, 737
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 5 cut(s) 40, 115, 199, 563, 852
MseI TTAA 4 cut(s) 117, 159, 288, 671
MspI CCGG 1 cut(s) 845
MspR9I CCNGG 1 cut(s) 234
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 5 cut(s) 101, 518, 644, 692, 788
NcoI CCATGG 1 cut(s) 361
NdeII GATC 3 cut(s) 135, 367, 934
NheI GCTAGC 2 cut(s) 485, 604
NlaIII CATG 6 cut(s) 350, 365, 445, 596, 617, 1035
NmuCI GTSAC 1 cut(s) 814
NspI RCATGY 2 cut(s) 596, 617
PciI ACATGT 2 cut(s) 592, 613
PfeI GAWTC 3 cut(s) 170, 334, 598
PkrI GCNGC 6 cut(s) 7, 10, 151, 430, 484, 640
PscI ACATGT 2 cut(s) 592, 613
PshAI GACNNNNGTC 1 cut(s) 821
Psp6I CCWGG 1 cut(s) 232
PspEI GGTNACC 1 cut(s) 980
PspFI CCCAGC 1 cut(s) 403
PspGI CCWGG 1 cut(s) 232
PspPI GGNCC 3 cut(s) 124, 830, 944
PstI CTGCAG 3 cut(s) 10, 76, 433
PstNI CAGNNNCTG 2 cut(s) 133, 644
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 422, 1022
RsaNI GTAC 2 cut(s) 421, 1021
SaqAI TTAA 4 cut(s) 117, 159, 288, 671
SatI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
Sau3AI GATC 3 cut(s) 135, 367, 934
Sau96I GGNCC 3 cut(s) 124, 830, 944
ScrFI CCNGG 1 cut(s) 234
SduI GDGCHC 2 cut(s) 184, 864
SfaNI GCATC 4 cut(s) 82, 120, 635, 745
SfcI CTRYAG 5 cut(s) 6, 72, 429, 870, 1002
SinI GGWCC 3 cut(s) 124, 830, 944
SmlI CTYRAG 1 cut(s) 986
SmoI CTYRAG 1 cut(s) 986
Sse9I AATT 4 cut(s) 86, 114, 165, 737
SsiI CCGC 4 cut(s) 533, 686, 791, 912
SspMI CTAG 3 cut(s) 486, 605, 996
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 2 cut(s) 361, 708
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 720
TasI AATT 4 cut(s) 86, 114, 165, 737
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 3 cut(s) 170, 334, 598
Tru1I TTAA 4 cut(s) 117, 159, 288, 671
Tru9I TTAA 4 cut(s) 117, 159, 288, 671
TscAI CASTG 2 cut(s) 789, 933
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 6 cut(s) 5, 8, 149, 428, 482, 638
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 1 cut(s) 315
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 2 cut(s) 789, 933
VpaK11BI GGWCC 3 cut(s) 124, 830, 944
XapI RAATTY 2 cut(s) 86, 737
XbaI TCTAGA 1 cut(s) 995
XceI RCATGY 2 cut(s) 596, 617
XspI CTAG 3 cut(s) 486, 605, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.