pycom111g02680

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001188
Physical Location & Seq
Reverse (-)
1613354 .. 1614914
1561 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02680.1

Sequence Viewer

Length: 876 bp
ATGGCGGCTGCTGTAGCTTCAAGTGATTCAGTTTCCATACCATCTGTAAACAAGGCGTGGGTGTACTCAGAGTACGGAAAGTCTGCAGATGTTTTGAAGTTCGATCCGAATGTTCCTGTTCCTGAAATAAAGGAAGACCAGGTGCTGATCAAGGTGGTTGCTGCATCTCTCAACCCAGTTGATTTCAAAAGGATGCTTGGCTACTTCAAGGACACTGACTCTTCACCCCCTACAGTTCCAGGGTATGATGTTGCTGGTGTGGTGGTGAAAGTAGGAAGACAGGTGACAAAGTTTAAGGTGGGGGATGATGTATATGGGGATCTCAACGAGAAGGCTCTCGATAAGCCGAAAAAGATCGGATCTTTGGCCGAGTACACTGCTGCAGAAGAAAGAGTGTTAGCTCTTAAGCCCAAAAATCTGAGTTTTGTTGAAGCTGCTAGCCTTCCCCTGGCCATTGAGACTGCCTATGAAGGGCTCGAACGAGTTGAATTCTCTGCCGGTAAATCCATCCTTGTTTTGGGAGGCGCTGGGGGAGTCGGAACACATGTTATTCAGCTAGCAAAGCATGTGTTTGGTGCTTCAAAAGTAGCAGCTACAGCGAGCACGAAAAAACTCGACCTTTTGAGAAGCTTGGGTGCTGATTTGGCTATTGATTACACCAAGGAGAACTTCGAAGACCTGCCCGAGCAATTCGATGTGGTTTATGATGCAGTTGGGGAGACTGACAGGGCGGTGAAGGCGGTGAAGGAAGGCGGGAAGGTTGTGACAATCGTAGGTCCAGTAACGCCACCGGCAATCATATTTATACTGACTTCAACCGGAACTGTATTAGAGAAACTGAAGCCTTACTTGGAGAGTGGGAAGGTGAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

292

Amino Acids

30.93

Weight (kDa)

5.63

Isoelectric Point (pI)

20.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 45 - 137 1.8e-16 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 151 - 250 5.7e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 178 - 258 4.7e-14 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 211 - 265 2.2e-08 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 687
AciI CCGC 4 cut(s) 5, 731, 740, 753
AclWI GGATC 3 cut(s) 98, 327, 367
AcoI YGGCCR 2 cut(s) 366, 450
AcsI RAATTY 1 cut(s) 488
AcuI CTGAAG 1 cut(s) 860
AfaI GTAC 3 cut(s) 65, 74, 374
AfiI CCNNNNNNNGG 3 cut(s) 448, 471, 517
AflII CTTAAG 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 544
AgsI TTSAA 8 cut(s) 21, 97, 187, 208, 431, 488, 582, 816
AjnI CCWGG 3 cut(s) 138, 238, 447
AjuI GAANNNNNNNTTGG 4 cut(s) 653, 685, 833, 865
AluBI AGCT 6 cut(s) 17, 401, 434, 556, 593, 630
AluI AGCT 6 cut(s) 17, 401, 434, 556, 593, 630
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 2 cut(s) 452, 713
AlwI GGATC 3 cut(s) 98, 327, 367
AlwNI CAGNNNCTG 1 cut(s) 145
Ama87I CYCGRG 1 cut(s) 683
AoxI GGCC 2 cut(s) 366, 450
ApeKI GCWGC 5 cut(s) 8, 161, 380, 434, 590
ApoI RAATTY 1 cut(s) 488
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 1 cut(s) 776
AsuHPI GGTGA 5 cut(s) 216, 277, 295, 745, 754
AsuII TTCGAA 1 cut(s) 672
AsuNHI GCTAGC 2 cut(s) 437, 556
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 1 cut(s) 776
BalI TGGCCA 1 cut(s) 452
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 3 cut(s) 141, 283, 681
Bbv12I GWGCWC 1 cut(s) 605
BbvI GCAGC 4 cut(s) 148, 367, 421, 602
BccI CCATC 2 cut(s) 49, 515
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 3 cut(s) 140, 240, 449
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 452, 713
BfaI CTAG 2 cut(s) 438, 557
BfmI CTRYAG 5 cut(s) 12, 84, 231, 381, 594
BfoI RGCGCY 1 cut(s) 528
BfrI CTTAAG 1 cut(s) 404
BfuAI ACCTGC 1 cut(s) 687
BisI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
BlsI GCNGC 6 cut(s) 7, 10, 163, 382, 436, 592
Bme1390I CCNGG 3 cut(s) 140, 240, 449
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 776
BmrFI CCNGG 3 cut(s) 140, 240, 449
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 3 cut(s) 173, 183, 697
BmtI GCTAGC 2 cut(s) 441, 560
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 3 cut(s) 141, 283, 681
Bpu14I TTCGAA 1 cut(s) 672
BsaJI CCNNGG 3 cut(s) 239, 447, 660
BsaWI WCCGGW 1 cut(s) 818
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 471, 517
Bse118I RCCGGY 2 cut(s) 497, 790
Bse1I ACTGG 2 cut(s) 176, 779
BseBI CCWGG 3 cut(s) 140, 240, 449
BseDI CCNNGG 3 cut(s) 239, 447, 660
BseGI GGATG 3 cut(s) 198, 310, 507
BseLI CCNNNNNNNGG 3 cut(s) 448, 471, 517
BseMII CTCAG 2 cut(s) 81, 410
BseNI ACTGG 2 cut(s) 176, 779
BseXI GCAGC 4 cut(s) 148, 367, 421, 602
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 2 cut(s) 368, 452
BsiHKAI GWGCWC 1 cut(s) 605
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 3 cut(s) 498, 791, 819
BslI CCNNNNNNNGG 3 cut(s) 448, 471, 517
BsmAI GTCTC 2 cut(s) 452, 713
BsnI GGCC 2 cut(s) 368, 452
BsoBI CYCGRG 1 cut(s) 683
Bsp119I TTCGAA 1 cut(s) 672
Bsp1286I GDGCHC 2 cut(s) 477, 605
Bsp143I GATC 5 cut(s) 103, 147, 319, 354, 359
BspACI CCGC 4 cut(s) 5, 731, 740, 753
BspANI GGCC 2 cut(s) 368, 452
BspCNI CTCAG 2 cut(s) 80, 411
BspMAI CTGCAG 2 cut(s) 88, 385
BspMI ACCTGC 1 cut(s) 687
BspOI GCTAGC 2 cut(s) 441, 560
BspPI GGATC 3 cut(s) 98, 327, 367
BspT104I TTCGAA 1 cut(s) 672
BspTI CTTAAG 1 cut(s) 404
BsrFI RCCGGY 2 cut(s) 497, 790
BsrI ACTGG 2 cut(s) 176, 779
BssAI RCCGGY 2 cut(s) 497, 790
BssECI CCNNGG 3 cut(s) 239, 447, 660
BssMI GATC 5 cut(s) 103, 147, 319, 354, 359
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 3 cut(s) 140, 240, 449
Bst4CI ACNGT 2 cut(s) 235, 826
Bst6I CTCTTC 1 cut(s) 226
BstAFI CTTAAG 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 672
BstC8I GCNNGC 3 cut(s) 439, 558, 601
BstDEI CTNAG 2 cut(s) 67, 419
BstF5I GGATG 3 cut(s) 198, 310, 507
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 5 cut(s) 106, 150, 322, 357, 362
BstMAI GTCTC 2 cut(s) 452, 713
BstMBI GATC 5 cut(s) 103, 147, 319, 354, 359
BstMWI GCNNNNNNNGC 5 cut(s) 14, 562, 596, 644, 737
BstNI CCWGG 3 cut(s) 140, 240, 449
BstNSI RCATGY 2 cut(s) 548, 569
BstSCI CCNGG 3 cut(s) 138, 238, 447
BstSFI CTRYAG 5 cut(s) 12, 84, 231, 381, 594
BstV1I GCAGC 4 cut(s) 148, 367, 421, 602
BstV2I GAAGAC 3 cut(s) 141, 283, 681
BstX2I RGATCY 2 cut(s) 319, 359
BstYI RGATCY 2 cut(s) 319, 359
BsuRI GGCC 2 cut(s) 368, 452
BtsCI GGATG 3 cut(s) 198, 310, 507
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 213, 375
BveI ACCTGC 1 cut(s) 687
Cac8I GCNNGC 3 cut(s) 439, 558, 601
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 1 cut(s) 527
Cfr10I RCCGGY 2 cut(s) 497, 790
Cfr13I GGNCC 1 cut(s) 776
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 3 cut(s) 64, 73, 373
CviAII CATG 2 cut(s) 545, 566
CviQI GTAC 3 cut(s) 64, 73, 373
DdeI CTNAG 2 cut(s) 67, 419
DpnI GATC 5 cut(s) 105, 149, 321, 356, 361
DpnII GATC 5 cut(s) 103, 147, 319, 354, 359
EaeI YGGCCR 2 cut(s) 366, 450
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 660
Eco24I GRGCYC 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 776
Eco57I CTGAAG 1 cut(s) 860
Eco88I CYCGRG 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 3 cut(s) 138, 238, 447
EcoT14I CCWWGG 1 cut(s) 660
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 2 cut(s) 548, 569
FalI AAGNNNNNCTT 4 cut(s) 653, 685, 833, 865
FatI CATG 2 cut(s) 544, 565
FauI CCCGC 1 cut(s) 746
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
FokI GGATG 3 cut(s) 205, 317, 494
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
FspBI CTAG 2 cut(s) 438, 557
GlaI GCGC 1 cut(s) 526
GluI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
GsaI CCCAGC 1 cut(s) 531
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 2 cut(s) 368, 452
HapII CCGG 3 cut(s) 498, 791, 819
HhaI GCGC 1 cut(s) 527
Hin1II CATG 2 cut(s) 548, 569
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 3 cut(s) 26, 218, 534
HpaII CCGG 3 cut(s) 498, 791, 819
HphI GGTGA 5 cut(s) 216, 277, 295, 745, 754
Hpy166II GTNNAC 3 cut(s) 49, 64, 375
Hpy188I TCNGA 5 cut(s) 70, 108, 359, 420, 539
Hpy188III TCNNGA 2 cut(s) 122, 338
Hpy8I GTNNAC 3 cut(s) 49, 64, 375
HpyAV CCTTC 8 cut(s) 325, 452, 464, 730, 739, 743, 751, 856
HpyCH4III ACNGT 2 cut(s) 235, 826
HpyCH4V TGCA 4 cut(s) 86, 164, 383, 710
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 562, 596, 644, 737
HpyF3I CTNAG 2 cut(s) 67, 419
Hsp92II CATG 2 cut(s) 548, 569
HspAI GCGC 1 cut(s) 525
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 5 cut(s) 103, 147, 319, 354, 359
Lsp1109I GCAGC 4 cut(s) 148, 367, 421, 602
LweI GCATC 3 cut(s) 173, 183, 697
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 2 cut(s) 438, 557
MaeIII GTNAC 3 cut(s) 283, 763, 781
MalI GATC 5 cut(s) 105, 149, 321, 356, 361
MboI GATC 5 cut(s) 103, 147, 319, 354, 359
MboII GAAGA 5 cut(s) 146, 213, 288, 398, 686
MflI RGATCY 2 cut(s) 319, 359
MhlI GDGCHC 2 cut(s) 477, 605
MlsI TGGCCA 1 cut(s) 452
MluCI AATT 2 cut(s) 488, 689
MluNI TGGCCA 1 cut(s) 452
MlyI GAGTC 2 cut(s) 212, 543
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 1 cut(s) 515
Mox20I TGGCCA 1 cut(s) 452
MscI TGGCCA 1 cut(s) 452
MseI TTAA 2 cut(s) 294, 405
Msp20I TGGCCA 1 cut(s) 452
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 3 cut(s) 498, 791, 819
MspR9I CCNGG 3 cut(s) 140, 240, 449
MvaI CCWGG 3 cut(s) 140, 240, 449
MwoI GCNNNNNNNGC 5 cut(s) 14, 562, 596, 644, 737
NdeII GATC 5 cut(s) 103, 147, 319, 354, 359
NheI GCTAGC 2 cut(s) 437, 556
NlaIII CATG 2 cut(s) 548, 569
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 2 cut(s) 283, 763
NspI RCATGY 2 cut(s) 548, 569
NspV TTCGAA 1 cut(s) 672
PciI ACATGT 1 cut(s) 544
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 6 cut(s) 7, 10, 163, 382, 436, 592
PleI GAGTC 2 cut(s) 212, 542
PpsI GAGTC 2 cut(s) 212, 542
PscI ACATGT 1 cut(s) 544
Psp6I CCWGG 3 cut(s) 138, 238, 447
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 3 cut(s) 138, 238, 447
PspPI GGNCC 1 cut(s) 776
PstI CTGCAG 2 cut(s) 88, 385
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 2 cut(s) 319, 359
RsaI GTAC 3 cut(s) 65, 74, 374
RsaNI GTAC 3 cut(s) 64, 73, 373
SaqAI TTAA 2 cut(s) 294, 405
SatI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
Sau3AI GATC 5 cut(s) 103, 147, 319, 354, 359
Sau96I GGNCC 1 cut(s) 776
SchI GAGTC 2 cut(s) 212, 543
ScrFI CCNGG 3 cut(s) 140, 240, 449
SduI GDGCHC 2 cut(s) 477, 605
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 3 cut(s) 173, 183, 697
SfcI CTRYAG 5 cut(s) 12, 84, 231, 381, 594
SfuI TTCGAA 1 cut(s) 672
SinI GGWCC 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 2 cut(s) 488, 689
SsiI CCGC 4 cut(s) 5, 731, 740, 753
SspMI CTAG 2 cut(s) 438, 557
StyD4I CCNGG 3 cut(s) 138, 238, 447
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 2 cut(s) 235, 826
TaqI TCGA 6 cut(s) 102, 339, 477, 615, 672, 693
TasI AATT 2 cut(s) 488, 689
TatI WGTACW 2 cut(s) 63, 372
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 2 cut(s) 294, 405
Tru9I TTAA 2 cut(s) 294, 405
TscAI CASTG 2 cut(s) 220, 382
TseFI GTSAC 2 cut(s) 283, 763
TseI GCWGC 5 cut(s) 8, 161, 380, 434, 590
Tsp45I GTSAC 2 cut(s) 283, 763
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 220, 382
Vha464I CTTAAG 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 776
XapI RAATTY 1 cut(s) 488
XceI RCATGY 2 cut(s) 548, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XspI CTAG 2 cut(s) 438, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.