RchiOBHm_Chr2g0172531

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001188
Physical Location & Seq
Forward (+)
85840439 .. 85842541
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53981

Sequence Viewer

Length: 1026 bp
ATGACTGCAGCTCCAAGTGAGTCCATACCCTCTGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAGTGTGGCTGCTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTTGTCGCTGCTTCTCTTAACCCCATTGATTTCCTGAGAGCTCTTGGTTTCTTCAAGGACATAGAGGGTGACACTCCCTTACCTACAGTTCCAGGGTATGATGTGGCTGGTGTGGTAGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAAGAATAGCTATGGGGATCTCAAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAGAGTACACTGCTGCAGGAGAAACCATGTTGGCTCTCAAACCCAAAAACCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCTATTGAGACAGCGTATGGAGCGCTTGAAAGAGCGGAGCTTTCGGCTGGTAAATCCGTCCTTGTTTTGGGAGGCGCTGGGGGTGTTGGGACACATGTGATTCAGCTAGCTAAGCATGTATTCGGGGCTTCCAAAGTAGCAGCTACTGCAAGCACTAAAAAACTGGATTTGTTGAGAAGCTTGGGTGCTGATTTGGCTATCGATTACACCAAGGAGAACATTGAAGACCTGCCAGAGAAATTTGATGTAGTCTATGATGCAGTTGGGCAGACTGACAAGGCAGTGAAGGCGGTGAAGGAAGGTGGGAAGGTAGTGACAATAGTAGGTCCAGTAACTCCTCCGGCGTTCAGATTTGTGGCCACCCCTACAGGGTCTATCTTGGAGAAACTGAAGCCTTACTTGGAGAGCGAGAAGGTGAAGCCAGTGCTTGATCCCACAGGTCCATATCCCTTTTCTAAAGTTGTTGAAGCACTTGGTTACCTTGAGACTTCAAAAGCTACAGGAAAGGTGGTTGTGTACCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

341

Amino Acids

36.11

Weight (kDa)

6.05

Isoelectric Point (pI)

25.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 152 8.1e-17 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 166 - 265 3.3e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 193 - 273 8.5e-15 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 226 - 337 8.2e-18 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 732
AccBSI CCGCTC 1 cut(s) 530
AciI CCGC 2 cut(s) 530, 785
AclWI GGATC 3 cut(s) 86, 375, 920
AcoI YGGCCR 1 cut(s) 852
AcsI RAATTY 2 cut(s) 86, 734
AcuI CTGAAG 1 cut(s) 905
AfaI GTAC 2 cut(s) 419, 1013
AfeI AGCGCT 1 cut(s) 519
AfiI CCNNNNNNNGG 2 cut(s) 562, 864
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 6 cut(s) 196, 476, 524, 719, 962, 987
AjnI CCWGG 2 cut(s) 126, 232
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 497, 974
AlwI GGATC 3 cut(s) 86, 375, 920
AlwNI CAGNNNCTG 2 cut(s) 133, 641
Aor51HI AGCGCT 1 cut(s) 519
AoxI GGCC 1 cut(s) 852
ApeKI GCWGC 6 cut(s) 8, 104, 149, 425, 479, 635
ApoI RAATTY 2 cut(s) 86, 734
AspLEI GCGC 2 cut(s) 520, 572
AspS9I GGNCC 3 cut(s) 124, 821, 935
AsuHPI GGTGA 4 cut(s) 221, 397, 799, 922
AsuNHI GCTAGC 2 cut(s) 482, 601
AvaII GGWCC 3 cut(s) 124, 821, 935
BalI TGGCCA 1 cut(s) 854
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 6 cut(s) 20, 91, 136, 412, 466, 647
BccI CCATC 1 cut(s) 1025
BciT130I CCWGG 2 cut(s) 128, 234
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 497, 974
BfaI CTAG 2 cut(s) 483, 602
BfmI CTRYAG 6 cut(s) 6, 72, 225, 426, 861, 993
BfoI RGCGCY 2 cut(s) 521, 573
BfuAI ACCTGC 1 cut(s) 732
BisI GCNGC 6 cut(s) 9, 105, 150, 426, 480, 636
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 6 cut(s) 10, 106, 151, 427, 481, 637
Bme1390I CCNGG 2 cut(s) 128, 234
Bme18I GGWCC 3 cut(s) 124, 821, 935
BmgT120I GGNCC 3 cut(s) 124, 821, 935
BmrFI CCNGG 2 cut(s) 128, 234
BmsI GCATC 1 cut(s) 742
BmtI GCTAGC 2 cut(s) 486, 605
BpiI GAAGAC 1 cut(s) 726
Bpu10I CCTNAGC 2 cut(s) 464, 493
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 2 cut(s) 356, 998
Bsa29I ATCGAT 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 233, 705
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 562, 864
Bse1I ACTGG 3 cut(s) 663, 824, 917
BseBI CCWGG 2 cut(s) 128, 234
BseCI ATCGAT 1 cut(s) 696
BseDI CCNNGG 2 cut(s) 233, 705
BseGI GGATG 2 cut(s) 304, 1017
BseLI CCNNNNNNNGG 2 cut(s) 562, 864
BseMII CTCAG 2 cut(s) 167, 455
BseNI ACTGG 3 cut(s) 663, 824, 917
BseRI GAGGAG 1 cut(s) 822
BseXI GCAGC 6 cut(s) 20, 91, 136, 412, 466, 647
BseYI CCCAGC 2 cut(s) 400, 572
BsgI GTGCAG 1 cut(s) 299
BshFI GGCC 1 cut(s) 854
BshVI ATCGAT 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 836
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 2 cut(s) 562, 864
BsmAI GTCTC 2 cut(s) 497, 974
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 1 cut(s) 854
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 4 cut(s) 91, 135, 367, 925
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 2 cut(s) 530, 785
BspANI GGCC 1 cut(s) 854
BspCNI CTCAG 2 cut(s) 168, 456
BspDI ATCGAT 1 cut(s) 696
BspMAI CTGCAG 3 cut(s) 10, 76, 430
BspMI ACCTGC 1 cut(s) 732
BspOI GCTAGC 2 cut(s) 486, 605
BspPI GGATC 3 cut(s) 86, 375, 920
BsrBI CCGCTC 1 cut(s) 530
BsrI ACTGG 3 cut(s) 663, 824, 917
BssECI CCNNGG 2 cut(s) 233, 705
BssMI GATC 4 cut(s) 91, 135, 367, 925
BssT1I CCWWGG 1 cut(s) 705
Bst2UI CCWGG 2 cut(s) 128, 234
Bst4CI ACNGT 1 cut(s) 229
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 3 cut(s) 484, 603, 646
BstDEI CTNAG 5 cut(s) 176, 351, 464, 493, 606
BstEII GGTNACC 1 cut(s) 971
BstF5I GGATG 2 cut(s) 304, 1017
BstH2I RGCGCY 2 cut(s) 521, 573
BstHHI GCGC 2 cut(s) 520, 572
BstKTI GATC 4 cut(s) 94, 138, 370, 928
BstMAI GTCTC 2 cut(s) 497, 974
BstMBI GATC 4 cut(s) 91, 135, 367, 925
BstMWI GCNNNNNNNGC 5 cut(s) 515, 607, 641, 689, 782
BstNI CCWGG 2 cut(s) 128, 234
BstNSI RCATGY 2 cut(s) 593, 614
BstPI GGTNACC 1 cut(s) 971
BstSCI CCNGG 2 cut(s) 126, 232
BstSFI CTRYAG 6 cut(s) 6, 72, 225, 426, 861, 993
BstV1I GCAGC 6 cut(s) 20, 91, 136, 412, 466, 647
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
Bsu15I ATCGAT 1 cut(s) 696
BsuRI GGCC 1 cut(s) 854
BsuTUI ATCGAT 1 cut(s) 696
BtsCI GGATG 2 cut(s) 304, 1017
BtsI GCAGTG 2 cut(s) 420, 783
BtsIMutI CAGTG 3 cut(s) 420, 783, 924
BveI ACCTGC 1 cut(s) 732
Cac8I GCNNGC 3 cut(s) 484, 603, 646
CaiI CAGNNNCTG 2 cut(s) 133, 641
CfoI GCGC 2 cut(s) 520, 572
Cfr13I GGNCC 3 cut(s) 124, 821, 935
ClaI ATCGAT 1 cut(s) 696
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 2 cut(s) 418, 1012
CviAII CATG 5 cut(s) 347, 439, 590, 611, 1023
CviQI GTAC 2 cut(s) 418, 1012
DdeI CTNAG 5 cut(s) 176, 351, 464, 493, 606
DpnI GATC 4 cut(s) 93, 137, 369, 927
DpnII GATC 4 cut(s) 91, 135, 367, 925
EaeI YGGCCR 1 cut(s) 852
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 705
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 3 cut(s) 124, 821, 935
Eco47III AGCGCT 1 cut(s) 519
Eco53kI GAGCTC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 905
Eco91I GGTNACC 1 cut(s) 971
EcoICRI GAGCTC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 971
EcoRII CCWGG 2 cut(s) 126, 232
EcoT14I CCWWGG 1 cut(s) 705
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 705
FaeI CATG 5 cut(s) 350, 442, 593, 614, 1026
FalI AAGNNNNNCTT 2 cut(s) 878, 910
FaqI GGGAC 1 cut(s) 598
FatI CATG 5 cut(s) 346, 438, 589, 610, 1022
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 6 cut(s) 9, 105, 150, 426, 480, 636
FokI GGATG 2 cut(s) 311, 1004
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 6 cut(s) 9, 105, 150, 426, 480, 636
FspBI CTAG 2 cut(s) 483, 602
GlaI GCGC 2 cut(s) 519, 571
GluI GCNGC 6 cut(s) 9, 105, 150, 426, 480, 636
GsaI CCCAGC 2 cut(s) 404, 576
HaeII RGCGCY 2 cut(s) 521, 573
HaeIII GGCC 1 cut(s) 854
HapII CCGG 1 cut(s) 836
HhaI GCGC 2 cut(s) 520, 572
Hin1II CATG 5 cut(s) 350, 442, 593, 614, 1026
Hin6I GCGC 2 cut(s) 518, 570
HinP1I GCGC 2 cut(s) 518, 570
HindIII AAGCTT 1 cut(s) 673
HinfI GANTC 3 cut(s) 20, 334, 595
HpaII CCGG 1 cut(s) 836
HphI GGTGA 4 cut(s) 221, 397, 799, 922
Hpy166II GTNNAC 2 cut(s) 420, 1012
Hpy188I TCNGA 3 cut(s) 58, 84, 845
Hpy188III TCNNGA 3 cut(s) 110, 175, 373
Hpy8I GTNNAC 2 cut(s) 420, 1012
HpyAV CCTTC 7 cut(s) 256, 497, 775, 784, 788, 796, 901
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 6 cut(s) 8, 74, 280, 428, 644, 755
HpyF10VI GCNNNNNNNGC 5 cut(s) 515, 607, 641, 689, 782
HpyF3I CTNAG 5 cut(s) 176, 351, 464, 493, 606
Hsp92II CATG 5 cut(s) 350, 442, 593, 614, 1026
HspAI GCGC 2 cut(s) 518, 570
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 4 cut(s) 91, 135, 367, 925
LmnI GCTCC 4 cut(s) 16, 112, 515, 532
Lsp1109I GCAGC 6 cut(s) 20, 91, 136, 412, 466, 647
LweI GCATC 1 cut(s) 742
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 2 cut(s) 483, 602
MaeIII GTNAC 4 cut(s) 209, 808, 826, 971
MalI GATC 4 cut(s) 93, 137, 369, 927
MbiI CCGCTC 1 cut(s) 530
MboI GATC 4 cut(s) 91, 135, 367, 925
MboII GAAGA 2 cut(s) 184, 731
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 184
MlsI TGGCCA 1 cut(s) 854
MluCI AATT 3 cut(s) 86, 114, 734
MluNI TGGCCA 1 cut(s) 854
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 5 cut(s) 40, 115, 199, 560, 843
Mox20I TGGCCA 1 cut(s) 854
MscI TGGCCA 1 cut(s) 854
MseI TTAA 3 cut(s) 117, 159, 288
Msp20I TGGCCA 1 cut(s) 854
MspI CCGG 1 cut(s) 836
MspR9I CCNGG 2 cut(s) 128, 234
MvaI CCWGG 2 cut(s) 128, 234
MwoI GCNNNNNNNGC 5 cut(s) 515, 607, 641, 689, 782
NdeII GATC 4 cut(s) 91, 135, 367, 925
NheI GCTAGC 2 cut(s) 482, 601
NlaIII CATG 5 cut(s) 350, 442, 593, 614, 1026
NmuCI GTSAC 2 cut(s) 209, 808
NspI RCATGY 2 cut(s) 593, 614
PciI ACATGT 1 cut(s) 589
PfeI GAWTC 2 cut(s) 334, 595
PkrI GCNGC 6 cut(s) 10, 106, 151, 427, 481, 637
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PscI ACATGT 1 cut(s) 589
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 126, 232
PspEI GGTNACC 1 cut(s) 971
PspFI CCCAGC 2 cut(s) 400, 572
PspGI CCWGG 2 cut(s) 126, 232
PspPI GGNCC 3 cut(s) 124, 821, 935
PstI CTGCAG 3 cut(s) 10, 76, 430
PstNI CAGNNNCTG 2 cut(s) 133, 641
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 419, 1013
RsaNI GTAC 2 cut(s) 418, 1012
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 117, 159, 288
SatI GCNGC 6 cut(s) 9, 105, 150, 426, 480, 636
Sau3AI GATC 4 cut(s) 91, 135, 367, 925
Sau96I GGNCC 3 cut(s) 124, 821, 935
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 2 cut(s) 128, 234
SduI GDGCHC 1 cut(s) 184
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 1 cut(s) 742
SfcI CTRYAG 6 cut(s) 6, 72, 225, 426, 861, 993
SinI GGWCC 3 cut(s) 124, 821, 935
SmlI CTYRAG 2 cut(s) 371, 977
SmoI CTYRAG 2 cut(s) 371, 977
Sse9I AATT 3 cut(s) 86, 114, 734
SsiI CCGC 2 cut(s) 530, 785
SspMI CTAG 2 cut(s) 483, 602
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 126, 232
StyI CCWWGG 1 cut(s) 705
TaaI ACNGT 1 cut(s) 229
TaqI TCGA 1 cut(s) 696
TasI AATT 3 cut(s) 86, 114, 734
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 2 cut(s) 334, 595
Tru1I TTAA 3 cut(s) 117, 159, 288
Tru9I TTAA 3 cut(s) 117, 159, 288
TscAI CASTG 3 cut(s) 427, 783, 924
TseFI GTSAC 2 cut(s) 209, 808
TseI GCWGC 6 cut(s) 8, 104, 149, 425, 479, 635
Tsp45I GTSAC 2 cut(s) 209, 808
TspDTI ATGAA 1 cut(s) 315
TspGWI ACGGA 1 cut(s) 541
TspRI CASTG 3 cut(s) 427, 783, 924
VpaK11BI GGWCC 3 cut(s) 124, 821, 935
XapI RAATTY 2 cut(s) 86, 734
XceI RCATGY 2 cut(s) 593, 614
XspI CTAG 2 cut(s) 483, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.