Rroxscaffold_2G00080590

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001188
Physical Location & Seq
Reverse (-)
3584904 .. 3587081
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00080590.1

Sequence Viewer

Length: 1248 bp
ATGGTCACTGCTTACGCTCATTTGGACAATGATCCATCCAGATGCATACCTCCCTTGATAAGATGGCGCGCAGCTCACACATTTTTCTTAACTTTCACCCTATTAGTTCTACAAAGCGCACTCTGGAAACGTCTGTCACTACAAGCTTTACTTTTTGCCCTACATCAATCATCAATGGCTGCAGCTCCAAGTGAGTGCATACCCTCCGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCGAGTGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTGGTTGCTGCTTCTCTTAACCCAATTGATTTCGTGAGAGCTCTTGGTCTCTACAAGGACATAGAGGGTGATACTACCTTACCTACAATTCCAGGGTATGATGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAAGAATAGCCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAGAGTACACTGCTGCAGGAGAAACCATGTTGGTTCTCAAACCTAAAAATCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCTATTGAGACTGCGTATGGAGCGCTTGAAAGAGCGGAGCTTTCGGCTGGTAAATCCGTCCTTGTTTTGGGAGGCGCTGGGGGTGTTGGAACACATGTGATTCAGACAATAGTAATTGAGAATATATACTTTTATGTGAGACAGCTAGCTAAACGTGTTTTTGGGGCATCCAAAGTAGCAGCTACTGCAAGCACTAAAAAACTGGATTTGTTAAGAAGCTTGGGTGCGGATTTGGCTATTGATTACACCAAGGAAAACATCGAAGACCTGCCAGAGAAATTTGATGTAGTCTATGATGCAGTTGGGCAGACTGACAAGGCAGTGAAGGCGGTGAAGGAAGGTGGGAAGGTAGTGACAATAGTCATAGGTCCAGTACTAACTCCTCCGGCGTTCAGCTTTGTGCTCACCCCTACAGGGTCTATCTTGGAGAAACTGAAGCCTTACTTGGAGAGCGGGAAGGTGAAGCCAGTGCTTGATCCCACAGGTCCGTATCCCTTTTCTAAAGTTGTTGAAGCACTTGGTTACCTTGAGACTTCTAGAGCTACAGGAAAGGTGGTTGTGTACCCTATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

415

Amino Acids

44.71

Weight (kDa)

7.01

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 99 - 211 6.6e-17 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 272 - 346 2.2e-11 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 298 - 411 4e-19 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 948
AccBSI CCGCTC 2 cut(s) 707, 1125
AccII CGCG 1 cut(s) 69
AciI CCGC 4 cut(s) 707, 899, 1001, 1125
AclWI GGATC 4 cut(s) 26, 260, 549, 1142
AcsI RAATTY 2 cut(s) 260, 950
AcuI CTGAAG 1 cut(s) 1127
AfaI GTAC 3 cut(s) 596, 1047, 1235
AfeI AGCGCT 1 cut(s) 696
AfiI CCNNNNNNNGG 3 cut(s) 213, 739, 1086
AflIII ACRYGT 2 cut(s) 766, 826
AgsI TTSAA 3 cut(s) 653, 701, 1184
AjnI CCWGG 2 cut(s) 300, 406
AjuI GAANNNNNNNTTGG 2 cut(s) 1100, 1132
Alw21I GWGCWC 2 cut(s) 358, 1077
Alw26I GTCTC 4 cut(s) 368, 674, 805, 1196
AlwI GGATC 4 cut(s) 26, 260, 549, 1142
AlwNI CAGNNNCTG 2 cut(s) 307, 857
Aor51HI AGCGCT 1 cut(s) 696
ApeKI GCWGC 7 cut(s) 71, 179, 182, 323, 602, 656, 851
ApoI RAATTY 2 cut(s) 260, 950
AspLEI GCGC 5 cut(s) 69, 71, 119, 697, 749
AspS9I GGNCC 3 cut(s) 298, 1040, 1157
AsuHPI GGTGA 7 cut(s) 88, 395, 445, 574, 1015, 1069, 1144
AsuNHI GCTAGC 2 cut(s) 659, 817
AvaII GGWCC 3 cut(s) 298, 1040, 1157
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 942
Bbv12I GWGCWC 2 cut(s) 358, 1077
BbvI GCAGC 7 cut(s) 83, 166, 194, 310, 589, 643, 863
BccI CCATC 2 cut(s) 43, 57
BciT130I CCWGG 2 cut(s) 302, 408
BciVI GTATCC 1 cut(s) 1173
BclI TGATCA 1 cut(s) 309
BcoDI GTCTC 4 cut(s) 368, 674, 805, 1196
BfaI CTAG 3 cut(s) 660, 818, 1209
BfmI CTRYAG 5 cut(s) 180, 246, 603, 1083, 1215
BfoI RGCGCY 2 cut(s) 698, 750
BfuAI ACCTGC 1 cut(s) 948
BfuI GTATCC 1 cut(s) 1173
BisI GCNGC 7 cut(s) 72, 180, 183, 324, 603, 657, 852
BlsI GCNGC 7 cut(s) 73, 181, 184, 325, 604, 658, 853
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 2 cut(s) 302, 408
Bme18I GGWCC 3 cut(s) 298, 1040, 1157
BmgT120I GGNCC 3 cut(s) 298, 1040, 1157
BmrFI CCNGG 2 cut(s) 302, 408
BmsI GCATC 3 cut(s) 32, 848, 958
BmtI GCTAGC 2 cut(s) 663, 821
BoxI GACNNNNGTC 1 cut(s) 1031
BpiI GAAGAC 1 cut(s) 942
Bpu10I CCTNAGC 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 1220
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 407, 535, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 739, 1086
Bse1I ACTGG 3 cut(s) 879, 1043, 1139
BseBI CCWGG 2 cut(s) 302, 408
BseDI CCNNGG 3 cut(s) 407, 535, 921
BseGI GGATG 3 cut(s) 35, 478, 839
BseLI CCNNNNNNNGG 3 cut(s) 213, 739, 1086
BseMII CTCAG 1 cut(s) 632
BseNI ACTGG 3 cut(s) 879, 1043, 1139
BsePI GCGCGC 1 cut(s) 67
BseRI GAGGAG 1 cut(s) 1044
BseXI GCAGC 7 cut(s) 83, 166, 194, 310, 589, 643, 863
BseYI CCCAGC 2 cut(s) 577, 749
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 69
BsiHKAI GWGCWC 2 cut(s) 358, 1077
BsiSI CCGG 1 cut(s) 1058
BslI CCNNNNNNNGG 3 cut(s) 213, 739, 1086
BsmAI GTCTC 4 cut(s) 368, 674, 805, 1196
Bso31I GGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 358, 1077
Bsp143I GATC 5 cut(s) 31, 265, 309, 541, 1147
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 4 cut(s) 707, 899, 1001, 1125
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 1 cut(s) 69
BspMAI CTGCAG 3 cut(s) 184, 250, 607
BspMI ACCTGC 1 cut(s) 948
BspOI GCTAGC 2 cut(s) 663, 821
BspPI GGATC 4 cut(s) 26, 260, 549, 1142
BspTNI GGTCTC 1 cut(s) 368
BsrBI CCGCTC 2 cut(s) 707, 1125
BsrI ACTGG 3 cut(s) 879, 1043, 1139
BssECI CCNNGG 3 cut(s) 407, 535, 921
BssHII GCGCGC 1 cut(s) 67
BssMI GATC 5 cut(s) 31, 265, 309, 541, 1147
BssT1I CCWWGG 2 cut(s) 535, 921
Bst2UI CCWGG 2 cut(s) 302, 408
BstAPI GCANNNNNTGC 1 cut(s) 857
BstC8I GCNNGC 4 cut(s) 69, 661, 819, 862
BstDEI CTNAG 3 cut(s) 525, 641, 670
BstDSI CCRYGG 1 cut(s) 535
BstEII GGTNACC 1 cut(s) 1193
BstF5I GGATG 3 cut(s) 35, 478, 839
BstFNI CGCG 1 cut(s) 69
BstH2I RGCGCY 2 cut(s) 698, 750
BstHHI GCGC 5 cut(s) 69, 71, 119, 697, 749
BstKTI GATC 5 cut(s) 34, 268, 312, 544, 1150
BstMAI GTCTC 4 cut(s) 368, 674, 805, 1196
BstMBI GATC 5 cut(s) 31, 265, 309, 541, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 692, 857, 905, 998
BstNI CCWGG 2 cut(s) 302, 408
BstNSI RCATGY 1 cut(s) 770
BstPAI GACNNNNGTC 1 cut(s) 1031
BstPI GGTNACC 1 cut(s) 1193
BstSCI CCNGG 2 cut(s) 300, 406
BstSFI CTRYAG 5 cut(s) 180, 246, 603, 1083, 1215
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 7 cut(s) 83, 166, 194, 310, 589, 643, 863
BstV2I GAAGAC 1 cut(s) 942
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BsuI GTATCC 1 cut(s) 1173
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 3 cut(s) 35, 478, 839
BtsI GCAGTG 3 cut(s) 6, 597, 999
BtsIMutI CAGTG 4 cut(s) 6, 597, 999, 1146
BveI ACCTGC 1 cut(s) 948
Cac8I GCNNGC 4 cut(s) 69, 661, 819, 862
CaiI CAGNNNCTG 2 cut(s) 307, 857
CfoI GCGC 5 cut(s) 69, 71, 119, 697, 749
Cfr13I GGNCC 3 cut(s) 298, 1040, 1157
CsiI ACCWGGT 1 cut(s) 300
Csp6I GTAC 3 cut(s) 595, 1046, 1234
CviAII CATG 5 cut(s) 521, 536, 616, 767, 1245
CviQI GTAC 3 cut(s) 595, 1046, 1234
DdeI CTNAG 3 cut(s) 525, 641, 670
DpnI GATC 5 cut(s) 33, 267, 311, 543, 1149
DpnII GATC 5 cut(s) 31, 265, 309, 541, 1147
Ecl136II GAGCTC 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 535, 921
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 3 cut(s) 298, 1040, 1157
Eco47III AGCGCT 1 cut(s) 696
Eco53kI GAGCTC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 1127
Eco91I GGTNACC 1 cut(s) 1193
EcoICRI GAGCTC 1 cut(s) 356
EcoO65I GGTNACC 1 cut(s) 1193
EcoRII CCWGG 2 cut(s) 300, 406
EcoT14I CCWWGG 2 cut(s) 535, 921
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 535, 921
FaeI CATG 5 cut(s) 524, 539, 619, 770, 1248
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 1100, 1132
FatI CATG 5 cut(s) 520, 535, 615, 766, 1244
FauI CCCGC 1 cut(s) 1118
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 7 cut(s) 72, 180, 183, 324, 603, 657, 852
FokI GGATG 3 cut(s) 22, 485, 826
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 7 cut(s) 72, 180, 183, 324, 603, 657, 852
FspBI CTAG 3 cut(s) 660, 818, 1209
GlaI GCGC 5 cut(s) 68, 70, 118, 696, 748
GluI GCNGC 7 cut(s) 72, 180, 183, 324, 603, 657, 852
GsaI CCCAGC 2 cut(s) 581, 753
HaeII RGCGCY 2 cut(s) 698, 750
HapII CCGG 1 cut(s) 1058
HhaI GCGC 5 cut(s) 69, 71, 119, 697, 749
Hin1II CATG 5 cut(s) 524, 539, 619, 770, 1248
Hin6I GCGC 5 cut(s) 67, 69, 117, 695, 747
HinP1I GCGC 5 cut(s) 67, 69, 117, 695, 747
HindIII AAGCTT 2 cut(s) 144, 889
HinfI GANTC 2 cut(s) 508, 772
HpaII CCGG 1 cut(s) 1058
HphI GGTGA 7 cut(s) 88, 395, 445, 574, 1015, 1069, 1144
Hpy166II GTNNAC 2 cut(s) 597, 1234
Hpy188I TCNGA 5 cut(s) 232, 258, 270, 642, 777
Hpy188III TCNNGA 5 cut(s) 39, 124, 284, 349, 1209
Hpy8I GTNNAC 2 cut(s) 597, 1234
HpyAV CCTTC 7 cut(s) 430, 674, 991, 1000, 1004, 1012, 1123
HpyCH4IV ACGT 2 cut(s) 130, 826
HpyCH4V TGCA 8 cut(s) 45, 182, 198, 248, 454, 605, 860, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 692, 857, 905, 998
HpyF3I CTNAG 3 cut(s) 525, 641, 670
HpySE526I ACGT 2 cut(s) 130, 826
Hsp92II CATG 5 cut(s) 524, 539, 619, 770, 1248
HspAI GCGC 5 cut(s) 67, 69, 117, 695, 747
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 5 cut(s) 31, 265, 309, 541, 1147
LmnI GCTCC 3 cut(s) 190, 692, 709
Lsp1109I GCAGC 7 cut(s) 83, 166, 194, 310, 589, 643, 863
LweI GCATC 3 cut(s) 32, 848, 958
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 3 cut(s) 660, 818, 1209
MaeII ACGT 2 cut(s) 130, 826
MaeIII GTNAC 4 cut(s) 4, 135, 1024, 1193
MalI GATC 5 cut(s) 33, 267, 311, 543, 1149
MbiI CCGCTC 2 cut(s) 707, 1125
MboI GATC 5 cut(s) 31, 265, 309, 541, 1147
MboII GAAGA 1 cut(s) 947
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 2 cut(s) 358, 1077
MluCI AATT 6 cut(s) 260, 288, 339, 402, 786, 950
MmeI TCCRAC 1 cut(s) 739
MnlI CCTC 6 cut(s) 60, 214, 289, 373, 737, 1065
Mph1103I ATGCAT 1 cut(s) 47
MseI TTAA 5 cut(s) 89, 291, 333, 462, 884
MslI CAYNNNNRTG 1 cut(s) 40
MspI CCGG 1 cut(s) 1058
MspR9I CCNGG 2 cut(s) 302, 408
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 2 cut(s) 302, 408
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 692, 857, 905, 998
NcoI CCATGG 1 cut(s) 535
NdeII GATC 5 cut(s) 31, 265, 309, 541, 1147
NheI GCTAGC 2 cut(s) 659, 817
NlaIII CATG 5 cut(s) 524, 539, 619, 770, 1248
NmuCI GTSAC 3 cut(s) 4, 135, 1024
NsiI ATGCAT 1 cut(s) 47
NspI RCATGY 1 cut(s) 770
PauI GCGCGC 1 cut(s) 67
PciI ACATGT 1 cut(s) 766
PfeI GAWTC 2 cut(s) 508, 772
PkrI GCNGC 7 cut(s) 73, 181, 184, 325, 604, 658, 853
PscI ACATGT 1 cut(s) 766
PshAI GACNNNNGTC 1 cut(s) 1031
Psp124BI GAGCTC 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 300, 406
PspEI GGTNACC 1 cut(s) 1193
PspFI CCCAGC 2 cut(s) 577, 749
PspGI CCWGG 2 cut(s) 300, 406
PspPI GGNCC 3 cut(s) 298, 1040, 1157
PstI CTGCAG 3 cut(s) 184, 250, 607
PstNI CAGNNNCTG 2 cut(s) 307, 857
PsuI RGATCY 1 cut(s) 541
PteI GCGCGC 1 cut(s) 67
RsaI GTAC 3 cut(s) 596, 1047, 1235
RsaNI GTAC 3 cut(s) 595, 1046, 1234
RseI CAYNNNNRTG 1 cut(s) 40
SacI GAGCTC 1 cut(s) 358
SaqAI TTAA 5 cut(s) 89, 291, 333, 462, 884
SatI GCNGC 7 cut(s) 72, 180, 183, 324, 603, 657, 852
Sau3AI GATC 5 cut(s) 31, 265, 309, 541, 1147
Sau96I GGNCC 3 cut(s) 298, 1040, 1157
ScaI AGTACT 1 cut(s) 1047
ScrFI CCNGG 2 cut(s) 302, 408
SduI GDGCHC 2 cut(s) 358, 1077
SexAI ACCWGGT 1 cut(s) 300
SfaNI GCATC 3 cut(s) 32, 848, 958
SfcI CTRYAG 5 cut(s) 180, 246, 603, 1083, 1215
SinI GGWCC 3 cut(s) 298, 1040, 1157
SmiMI CAYNNNNRTG 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 1199
SmoI CTYRAG 1 cut(s) 1199
Sse9I AATT 6 cut(s) 260, 288, 339, 402, 786, 950
SsiI CCGC 4 cut(s) 707, 899, 1001, 1125
SspMI CTAG 3 cut(s) 660, 818, 1209
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 300, 406
StyI CCWWGG 2 cut(s) 535, 921
TaiI ACGT 2 cut(s) 133, 829
TaqI TCGA 1 cut(s) 933
TasI AATT 6 cut(s) 260, 288, 339, 402, 786, 950
TatI WGTACW 2 cut(s) 594, 1045
TfiI GAWTC 2 cut(s) 508, 772
Tru1I TTAA 5 cut(s) 89, 291, 333, 462, 884
Tru9I TTAA 5 cut(s) 89, 291, 333, 462, 884
TscAI CASTG 4 cut(s) 13, 604, 999, 1146
TseFI GTSAC 3 cut(s) 4, 135, 1024
TseI GCWGC 7 cut(s) 71, 179, 182, 323, 602, 656, 851
Tsp45I GTSAC 3 cut(s) 4, 135, 1024
TspDTI ATGAA 1 cut(s) 489
TspGWI ACGGA 3 cut(s) 196, 718, 1149
TspRI CASTG 4 cut(s) 13, 604, 999, 1146
VpaK11BI GGWCC 3 cut(s) 298, 1040, 1157
XapI RAATTY 2 cut(s) 260, 950
XbaI TCTAGA 1 cut(s) 1208
XceI RCATGY 1 cut(s) 770
XspI CTAG 3 cut(s) 660, 818, 1209
ZrmI AGTACT 1 cut(s) 1047
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.