Rroxscaffold_2G00080620

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018917
Physical Location & Seq
Reverse (-)
3596458 .. 3597397
940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00080620.1

Sequence Viewer

Length: 492 bp
ATGGCTGCAGCTCCAAGTGATTCCATACCCTCTGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAATGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTAGTTGCTGCTTCTCTTAACCCAATTGATTTCATGAGAGCTCCTAGTTTCTTCAAGGACATAGAGGGTGATAGTCCCTTACCTGTGGACAATTCCAGGTATGATGTGGCTAGTGTGGTGGTGAAGGTAGAAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAAGAATAGTCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAGGGTACACTGCTGCAGGAGAAACCATGTTGGCTCTCAAACCCAAAAATCTGAGCTTTATTGAAGCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

163

Amino Acids

17.68

Weight (kDa)

5.02

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 154 3.6e-10 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018917)

Species Orthologous Gene IDs
prunus_persica Prupe.3G284400_v2.0.a1
rosa_laevigata RLG00000022017
rosa_multiflora Rmu_sc0006783.1_g000003
rosa_roxburghii Rroxscaffold_2G00080620
rosa_rugosa Rorug02G0554800
rosa_samantha Rh2AG628900 Rh2AG635700 Rh2BG649100 Rh2CG608000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 86, 378
AcsI RAATTY 1 cut(s) 86
AfaI GTAC 1 cut(s) 425
AgsI TTSAA 2 cut(s) 196, 482
AjnI CCWGG 2 cut(s) 126, 236
AluBI AGCT 3 cut(s) 11, 182, 474
AluI AGCT 3 cut(s) 11, 182, 474
Alw21I GWGCWC 1 cut(s) 184
AlwI GGATC 2 cut(s) 86, 378
AlwNI CAGNNNCTG 1 cut(s) 133
ApeKI GCWGC 4 cut(s) 5, 8, 149, 431
ApoI RAATTY 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 3 cut(s) 221, 274, 403
AvaII GGWCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 3 cut(s) 20, 136, 418
BciT130I CCWGG 2 cut(s) 128, 238
BclI TGATCA 1 cut(s) 135
BfaI CTAG 3 cut(s) 186, 252, 490
BfmI CTRYAG 3 cut(s) 6, 72, 432
BisI GCNGC 4 cut(s) 6, 9, 150, 432
BlsI GCNGC 4 cut(s) 7, 10, 151, 433
Bme1390I CCNGG 2 cut(s) 128, 238
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 124
BmrFI CCNGG 2 cut(s) 128, 238
BseBI CCWGG 2 cut(s) 128, 238
BseGI GGATG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 461
BseXI GCAGC 3 cut(s) 20, 136, 418
BseYI CCCAGC 1 cut(s) 406
BsgI GTGCAG 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 184
BslFI GGGAC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 3 cut(s) 91, 135, 370
BspCNI CTCAG 1 cut(s) 462
BspHI TCATGA 1 cut(s) 174
BspMAI CTGCAG 3 cut(s) 10, 76, 436
BspPI GGATC 2 cut(s) 86, 378
BssMI GATC 3 cut(s) 91, 135, 370
Bst2UI CCWGG 2 cut(s) 128, 238
BstC8I GCNNGC 1 cut(s) 487
BstDEI CTNAG 2 cut(s) 354, 470
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 3 cut(s) 94, 138, 373
BstMBI GATC 3 cut(s) 91, 135, 370
BstNI CCWGG 2 cut(s) 128, 238
BstNSI RCATGY 1 cut(s) 489
BstSCI CCNGG 2 cut(s) 126, 236
BstSFI CTRYAG 3 cut(s) 6, 72, 432
BstV1I GCAGC 3 cut(s) 20, 136, 418
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 487
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 1 cut(s) 424
CviAII CATG 5 cut(s) 175, 350, 365, 445, 486
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 2 cut(s) 354, 470
DpnI GATC 3 cut(s) 93, 137, 372
DpnII GATC 3 cut(s) 91, 135, 370
Ecl136II GAGCTC 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 126, 236
EcoT38I GRGCYC 1 cut(s) 184
FaeI CATG 5 cut(s) 178, 353, 368, 448, 489
FaqI GGGAC 1 cut(s) 201
FatI CATG 5 cut(s) 174, 349, 364, 444, 485
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 6, 9, 150, 432
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 4 cut(s) 6, 9, 150, 432
FspBI CTAG 3 cut(s) 186, 252, 490
GluI GCNGC 4 cut(s) 6, 9, 150, 432
GsaI CCCAGC 1 cut(s) 410
Hin1II CATG 5 cut(s) 178, 353, 368, 448, 489
HinfI GANTC 2 cut(s) 20, 337
HphI GGTGA 3 cut(s) 221, 274, 403
Hpy166II GTNNAC 2 cut(s) 229, 426
Hpy188I TCNGA 3 cut(s) 58, 84, 471
Hpy188III TCNNGA 2 cut(s) 110, 175
Hpy8I GTNNAC 2 cut(s) 229, 426
HpyAV CCTTC 1 cut(s) 259
HpyCH4V TGCA 4 cut(s) 8, 74, 283, 434
HpyF3I CTNAG 2 cut(s) 354, 470
Hsp92II CATG 5 cut(s) 178, 353, 368, 448, 489
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 3 cut(s) 91, 135, 370
LmnI GCTCC 2 cut(s) 16, 187
Lsp1109I GCAGC 3 cut(s) 20, 136, 418
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 3 cut(s) 186, 252, 490
MalI GATC 3 cut(s) 93, 137, 372
MboI GATC 3 cut(s) 91, 135, 370
MboII GAAGA 1 cut(s) 184
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 4 cut(s) 86, 114, 165, 232
MnlI CCTC 3 cut(s) 40, 115, 199
MseI TTAA 3 cut(s) 117, 159, 291
MspR9I CCNGG 2 cut(s) 128, 238
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 2 cut(s) 128, 238
NdeII GATC 3 cut(s) 91, 135, 370
NlaIII CATG 5 cut(s) 178, 353, 368, 448, 489
NspI RCATGY 1 cut(s) 489
PaeI GCATGC 1 cut(s) 489
PagI TCATGA 1 cut(s) 174
PfeI GAWTC 2 cut(s) 20, 337
PkrI GCNGC 4 cut(s) 7, 10, 151, 433
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 126, 236
PspFI CCCAGC 1 cut(s) 406
PspGI CCWGG 2 cut(s) 126, 236
PspPI GGNCC 1 cut(s) 124
PstI CTGCAG 3 cut(s) 10, 76, 436
PstNI CAGNNNCTG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 117, 159, 291
SatI GCNGC 4 cut(s) 6, 9, 150, 432
Sau3AI GATC 3 cut(s) 91, 135, 370
Sau96I GGNCC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 128, 238
SduI GDGCHC 1 cut(s) 184
SetI ASST 9 cut(s) 13, 132, 144, 184, 226, 242, 270, 297, 476
SexAI ACCWGGT 1 cut(s) 126
SfcI CTRYAG 3 cut(s) 6, 72, 432
SinI GGWCC 1 cut(s) 124
SphI GCATGC 1 cut(s) 489
Sse9I AATT 4 cut(s) 86, 114, 165, 232
SspMI CTAG 3 cut(s) 186, 252, 490
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 126, 236
TasI AATT 4 cut(s) 86, 114, 165, 232
TfiI GAWTC 2 cut(s) 20, 337
Tru1I TTAA 3 cut(s) 117, 159, 291
Tru9I TTAA 3 cut(s) 117, 159, 291
TscAI CASTG 1 cut(s) 433
TseI GCWGC 4 cut(s) 5, 8, 149, 431
TspDTI ATGAA 2 cut(s) 163, 318
TspRI CASTG 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XspI CTAG 3 cut(s) 186, 252, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.