Rh2BG649100

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018917
Physical Location & Seq
Reverse (-)
86793922 .. 86795061
1140 bp
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UTR
Exon/CDS
Intron
Rh2BG649100.1

Sequence Viewer

Length: 387 bp
ATGACTGCAGCTCCAAGTGAGTCCATACCCTCTGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAGTGTGACTGCTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTTGTCGCTGCTTCTCTTAACCCAATTGATTTCATGAGAGCTCTTGGTTTCTTCAAGGACATAGAGGGTGACACTCCCTTACCTACAGTTCCAGGGTATGATGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCACTGAGATTCCCAATATCATGCTAAGAATAGCCATGGGGATCTCAAGAAAGCATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

128

Amino Acids

13.92

Weight (kDa)

5.06

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 109 7.8e-16 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018917)

Species Orthologous Gene IDs
prunus_persica Prupe.3G284400_v2.0.a1
rosa_laevigata RLG00000022017
rosa_multiflora Rmu_sc0006783.1_g000003
rosa_roxburghii Rroxscaffold_2G00080620
rosa_rugosa Rorug02G0554800
rosa_samantha Rh2AG628900 Rh2AG635700 Rh2BG649100 Rh2CG608000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 86, 374
AcsI RAATTY 1 cut(s) 86
AgsI TTSAA 1 cut(s) 196
AjnI CCWGG 2 cut(s) 126, 232
AluBI AGCT 2 cut(s) 11, 182
AluI AGCT 2 cut(s) 11, 182
Alw21I GWGCWC 1 cut(s) 184
AlwI GGATC 2 cut(s) 86, 374
AlwNI CAGNNNCTG 1 cut(s) 133
ApeKI GCWGC 2 cut(s) 8, 149
ApoI RAATTY 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 221, 271
AvaII GGWCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 2 cut(s) 20, 136
BciT130I CCWGG 2 cut(s) 128, 234
BclI TGATCA 1 cut(s) 135
BfmI CTRYAG 3 cut(s) 6, 72, 225
BisI GCNGC 2 cut(s) 9, 150
BlsI GCNGC 2 cut(s) 10, 151
Bme1390I CCNGG 2 cut(s) 128, 234
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 124
BmrFI CCNGG 2 cut(s) 128, 234
BpuEI CTTGAG 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 233, 360
BsaXI ACNNNNNCTCC 1 cut(s) 25
BseBI CCWGG 2 cut(s) 128, 234
BseDI CCNNGG 2 cut(s) 233, 360
BseGI GGATG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 321
BseXI GCAGC 2 cut(s) 20, 136
BsgI GTGCAG 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 3 cut(s) 91, 135, 366
Bsp19I CCATGG 1 cut(s) 360
BspCNI CTCAG 1 cut(s) 322
BspHI TCATGA 1 cut(s) 174
BspMAI CTGCAG 2 cut(s) 10, 76
BspPI GGATC 2 cut(s) 86, 374
BssECI CCNNGG 2 cut(s) 233, 360
BssMI GATC 3 cut(s) 91, 135, 366
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 2 cut(s) 128, 234
Bst4CI ACNGT 1 cut(s) 229
BstDEI CTNAG 2 cut(s) 330, 350
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 3 cut(s) 94, 138, 369
BstMBI GATC 3 cut(s) 91, 135, 366
BstNI CCWGG 2 cut(s) 128, 234
BstSCI CCNGG 2 cut(s) 126, 232
BstSFI CTRYAG 3 cut(s) 6, 72, 225
BstV1I GCAGC 2 cut(s) 20, 136
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 327
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 126
CviAII CATG 3 cut(s) 175, 346, 361
CviJI RGCY 6 cut(s) 11, 44, 182, 248, 273, 359
CviKI_1 RGCY 6 cut(s) 11, 44, 182, 248, 273, 359
DdeI CTNAG 2 cut(s) 330, 350
DpnI GATC 3 cut(s) 93, 137, 368
DpnII GATC 3 cut(s) 91, 135, 366
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 126, 232
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 3 cut(s) 178, 349, 364
FatI CATG 3 cut(s) 174, 345, 360
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 9, 150
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 2 cut(s) 9, 150
GluI GCNGC 2 cut(s) 9, 150
Hin1II CATG 3 cut(s) 178, 349, 364
HinfI GANTC 2 cut(s) 20, 334
HphI GGTGA 2 cut(s) 221, 271
Hpy188I TCNGA 2 cut(s) 58, 84
Hpy188III TCNNGA 3 cut(s) 110, 175, 372
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 3 cut(s) 8, 74, 280
HpyF3I CTNAG 2 cut(s) 330, 350
Hsp92II CATG 3 cut(s) 178, 349, 364
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 3 cut(s) 91, 135, 366
LmnI GCTCC 2 cut(s) 16, 112
LpnPI CCDG 6 cut(s) 113, 123, 140, 219, 234, 246
Lsp1109I GCAGC 2 cut(s) 20, 136
MabI ACCWGGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 100, 209
MalI GATC 3 cut(s) 93, 137, 368
MboI GATC 3 cut(s) 91, 135, 366
MboII GAAGA 1 cut(s) 184
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 3 cut(s) 86, 114, 165
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 3 cut(s) 40, 115, 199
MseI TTAA 3 cut(s) 117, 159, 288
MspR9I CCNGG 2 cut(s) 128, 234
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 2 cut(s) 128, 234
NcoI CCATGG 1 cut(s) 360
NdeII GATC 3 cut(s) 91, 135, 366
NlaIII CATG 3 cut(s) 178, 349, 364
NmuCI GTSAC 2 cut(s) 100, 209
PagI TCATGA 1 cut(s) 174
PfeI GAWTC 1 cut(s) 334
PkrI GCNGC 2 cut(s) 10, 151
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 126, 232
PspGI CCWGG 2 cut(s) 126, 232
PspPI GGNCC 1 cut(s) 124
PstI CTGCAG 2 cut(s) 10, 76
PstNI CAGNNNCTG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 366
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 117, 159, 288
SatI GCNGC 2 cut(s) 9, 150
Sau3AI GATC 3 cut(s) 91, 135, 366
Sau96I GGNCC 1 cut(s) 124
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 2 cut(s) 128, 234
SduI GDGCHC 1 cut(s) 184
SetI ASST 7 cut(s) 13, 132, 144, 184, 226, 267, 294
SexAI ACCWGGT 1 cut(s) 126
SfcI CTRYAG 3 cut(s) 6, 72, 225
SinI GGWCC 1 cut(s) 124
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 3 cut(s) 86, 114, 165
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 126, 232
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 1 cut(s) 229
TasI AATT 3 cut(s) 86, 114, 165
TfiI GAWTC 1 cut(s) 334
Tru1I TTAA 3 cut(s) 117, 159, 288
Tru9I TTAA 3 cut(s) 117, 159, 288
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 100, 209
TseI GCWGC 2 cut(s) 8, 149
Tsp45I GTSAC 2 cut(s) 100, 209
TspDTI ATGAA 2 cut(s) 163, 315
TspRI CASTG 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.