RLG00000022945

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017973
Physical Location & Seq
Reverse (-)
15553508 .. 15555019
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022945

Sequence Viewer

Length: 1032 bp
ATGGTTCCCCTCAAGCAATATAGAAGAAGAAGAAGAAGAAGACCCCCCACTGAGATCAAAGACTTGACCCCTGACATATTGTTTGAAATCTTTGCAAGAGTGCCCATGAAATATCTTTTGCAGTTAGCAACAGTTTGCAAATCTTACAAGGCTTTAATTAGGAGCCATGAGTTCAGAAGATTCCATCACGAAAAGAATACCATGAAAAATGCTCCTGATTATCTACTTGTTCGCAATAGAATTGGAGGTCAACAAAGGGGCTTCTCCATTTATTCTGCTAGAACATTTGCCTATGATGAGAATTCAGGGCTTCCTAGGTCCATAGATATTCCCATGCAGATGCAGCTCCATGACCTTCCTCTGTACGAATATGGTTTCACTGTTTATGGTTCTTGCAATGGATTGCTTTGCATCTCTTTATTCTCTCTGGACTTGGACTCTCCTTTATATCTGTACAATCCATCACTCAGAAAATTCAAGCAACTTCCTCGAAGTGAATTTGAGCTTCCTCAAGGCACCTCCATTACTTCTCAGATAGCCACAGGTTTAGTTACTCTCGGCTTTGGTTTCCATTCTGGGAAGGATGACTATCAGGTTGTGAGGTTTGTACACCCCAATACAAGTATCTTTTATGCTGAGGTTTACAGTCTCAAGTTGAATTCTTGGACACCAGTAGAGAATGTTGTAGATCCCCGTGAGGTTGATGCTTGGTTTATGGAAGGATGTACATGCTTGGATGGAGTTATATATTGGCATTTAATGCAGCGTCCTAACATGTCCCTCGTTTCTTTTAATATGCACACTTCAGTTCTTGGAAAGAGGACGCTTCCTTATCAGCTGCAACCGGTGATGCGTCCAATTTGTTTGCAAGTGTTGCATCCAGAATGTTTGCGAGCGCTGCATGAAAATAGTACTGTCTATGTAGTTCATATGGTTTTCTATTTTGCCGGTCCATATTTGGTTTCATTTGTTCCTCTGCTGCATAGACATCCGACTGGAAATTCAACCAAGTCACAGGGAGTTTCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

344

Amino Acids

39.7

Weight (kDa)

9.3

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 60 1.8e-08 F-box domain
FBA_3 PF08268 124 - 268 2.8e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017973)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27470
rosa_chinensis RchiOBHm_Chr3g0488541
rosa_laevigata RLG00000022945
rosa_multiflora Rmu_co8412829.1_g000001
rosa_roxburghii Rroxscaffold_6G00394190
rosa_rugosa Rorug03G0239100
rosa_samantha Rh3AG287900 Rh3BG324800 Rh3CG322000 Rh3DG320900
rosa_wichuraiana Rw3G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 515
AclWI GGATC 1 cut(s) 683
AcsI RAATTY 5 cut(s) 301, 473, 497, 658, 1000
AcuI CTGAAG 1 cut(s) 789
AfaI GTAC 5 cut(s) 365, 455, 609, 727, 913
AfeI AGCGCT 1 cut(s) 897
AflIII ACRYGT 1 cut(s) 774
AgeI ACCGGT 1 cut(s) 844
AgsI TTSAA 4 cut(s) 86, 478, 658, 1005
AluBI AGCT 3 cut(s) 346, 505, 838
AluI AGCT 3 cut(s) 346, 505, 838
Alw26I GTCTC 1 cut(s) 653
AlwI GGATC 1 cut(s) 683
Aor51HI AGCGCT 1 cut(s) 897
ApeKI GCWGC 5 cut(s) 343, 763, 838, 898, 979
ApoI RAATTY 5 cut(s) 301, 473, 497, 658, 1000
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AsiGI ACCGGT 1 cut(s) 844
AspA2I CCTAGG 1 cut(s) 314
AspLEI GCGC 1 cut(s) 898
AspS9I GGNCC 2 cut(s) 318, 950
AsuHPI GGTGA 1 cut(s) 859
AvaII GGWCC 2 cut(s) 318, 950
AvrII CCTAGG 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 105
BanI GGYRCC 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 46
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 5 cut(s) 355, 775, 825, 885, 966
BccI CCATC 3 cut(s) 192, 469, 731
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 3 cut(s) 279, 315, 1026
BfoI RGCGCY 1 cut(s) 899
BisI GCNGC 5 cut(s) 344, 764, 839, 899, 980
BlnI CCTAGG 1 cut(s) 314
BlsI GCNGC 5 cut(s) 345, 765, 840, 900, 981
BmcAI AGTACT 1 cut(s) 913
Bme18I GGWCC 2 cut(s) 318, 950
BmgT120I GGNCC 2 cut(s) 318, 950
BmiI GGNNCC 3 cut(s) 6, 164, 517
BmsI GCATC 5 cut(s) 330, 420, 694, 840, 886
BpiI GAAGAC 1 cut(s) 46
Bpu10I CCTNAGC 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 495, 635
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 844
Bse118I RCCGGY 2 cut(s) 844, 947
Bse1I ACTGG 2 cut(s) 671, 1000
Bse3DI GCAATG 1 cut(s) 403
Bse8I GATNNNNATC 1 cut(s) 588
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 5 cut(s) 589, 728, 742, 877, 988
BseJI GATNNNNATC 1 cut(s) 588
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 4 cut(s) 42, 481, 545, 627
BseNI ACTGG 2 cut(s) 671, 1000
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 5 cut(s) 355, 775, 825, 885, 966
BshNI GGYRCC 1 cut(s) 515
BshTI ACCGGT 1 cut(s) 844
BsiSI CCGG 2 cut(s) 845, 948
BslFI GGGAC 1 cut(s) 763
BsmAI GTCTC 1 cut(s) 653
BsmFI GGGAC 1 cut(s) 763
Bsp1286I GDGCHC 1 cut(s) 105
Bsp1407I TGTACA 3 cut(s) 453, 607, 725
Bsp143I GATC 2 cut(s) 54, 688
BspCNI CTCAG 4 cut(s) 43, 480, 544, 628
BspLI GGNNCC 3 cut(s) 6, 164, 517
BspPI GGATC 1 cut(s) 683
BspT107I GGYRCC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 403
BsrFI RCCGGY 2 cut(s) 844, 947
BsrGI TGTACA 3 cut(s) 453, 607, 725
BsrI ACTGG 2 cut(s) 671, 1000
BssAI RCCGGY 2 cut(s) 844, 947
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 2 cut(s) 54, 688
BssT1I CCWWGG 1 cut(s) 314
Bst4CI ACNGT 4 cut(s) 133, 382, 647, 916
BstAPI GCANNNNNTGC 2 cut(s) 760, 874
BstAUI TGTACA 3 cut(s) 453, 607, 725
BstC8I GCNNGC 1 cut(s) 894
BstDEI CTNAG 4 cut(s) 51, 467, 531, 636
BstF5I GGATG 5 cut(s) 589, 728, 742, 877, 988
BstH2I RGCGCY 1 cut(s) 899
BstHHI GCGC 1 cut(s) 898
BstKTI GATC 2 cut(s) 57, 691
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 2 cut(s) 54, 688
BstMWI GCNNNNNNNGC 4 cut(s) 343, 760, 874, 898
BstNSI RCATGY 2 cut(s) 732, 778
BstSLI GKGCMC 1 cut(s) 105
BstV1I GCAGC 5 cut(s) 355, 775, 825, 885, 966
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
BtsCI GGATG 5 cut(s) 589, 728, 742, 877, 988
BtsIMutI CAGTG 2 cut(s) 48, 378
Cac8I GCNNGC 1 cut(s) 894
CfoI GCGC 1 cut(s) 898
Cfr10I RCCGGY 2 cut(s) 844, 947
Cfr13I GGNCC 2 cut(s) 318, 950
CseI GACGC 3 cut(s) 755, 832, 842
Csp6I GTAC 5 cut(s) 364, 454, 608, 726, 912
CspAI ACCGGT 1 cut(s) 844
CviAII CATG 8 cut(s) 106, 167, 202, 334, 350, 729, 775, 902
CviJI RGCY 9 cut(s) 152, 165, 261, 310, 346, 505, 539, 561, 838
CviKI_1 RGCY 9 cut(s) 152, 165, 261, 310, 346, 505, 539, 561, 838
CviQI GTAC 5 cut(s) 364, 454, 608, 726, 912
DdeI CTNAG 4 cut(s) 51, 467, 531, 636
DpnI GATC 2 cut(s) 56, 690
DpnII GATC 2 cut(s) 54, 688
Eco130I CCWWGG 1 cut(s) 314
Eco47I GGWCC 2 cut(s) 318, 950
Eco47III AGCGCT 1 cut(s) 897
Eco57I CTGAAG 1 cut(s) 789
EcoRI GAATTC 2 cut(s) 301, 658
EcoT14I CCWWGG 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 8 cut(s) 109, 170, 205, 337, 353, 732, 778, 905
FaqI GGGAC 1 cut(s) 763
FatI CATG 8 cut(s) 105, 166, 201, 333, 349, 728, 774, 901
FauNDI CATATG 1 cut(s) 930
Fnu4HI GCNGC 5 cut(s) 344, 764, 839, 899, 980
FokI GGATG 5 cut(s) 596, 735, 749, 864, 975
Fsp4HI GCNGC 5 cut(s) 344, 764, 839, 899, 980
FspBI CTAG 3 cut(s) 279, 315, 1026
GlaI GCGC 1 cut(s) 897
GluI GCNGC 5 cut(s) 344, 764, 839, 899, 980
HaeII RGCGCY 1 cut(s) 899
HapII CCGG 2 cut(s) 845, 948
HgaI GACGC 3 cut(s) 755, 832, 842
HhaI GCGC 1 cut(s) 898
Hin1II CATG 8 cut(s) 109, 170, 205, 337, 353, 732, 778, 905
Hin6I GCGC 1 cut(s) 896
HinP1I GCGC 1 cut(s) 896
HincII GTYRAC 1 cut(s) 251
HindII GTYRAC 1 cut(s) 251
HinfI GANTC 2 cut(s) 180, 437
HpaII CCGG 2 cut(s) 845, 948
HphI GGTGA 1 cut(s) 859
Hpy166II GTNNAC 3 cut(s) 251, 610, 643
Hpy188I TCNGA 4 cut(s) 176, 470, 534, 993
Hpy188III TCNNGA 4 cut(s) 188, 215, 428, 881
Hpy8I GTNNAC 3 cut(s) 251, 610, 643
HpyAV CCTTC 3 cut(s) 365, 574, 713
HpyCH4III ACNGT 4 cut(s) 133, 382, 647, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 343, 760, 874, 898
HpyF3I CTNAG 4 cut(s) 51, 467, 531, 636
Hsp92II CATG 8 cut(s) 109, 170, 205, 337, 353, 732, 778, 905
HspAI GCGC 1 cut(s) 896
Kzo9I GATC 2 cut(s) 54, 688
LmnI GCTCC 3 cut(s) 162, 217, 351
Lsp1109I GCAGC 5 cut(s) 355, 775, 825, 885, 966
LweI GCATC 5 cut(s) 330, 420, 694, 840, 886
MaeI CTAG 3 cut(s) 279, 315, 1026
MaeIII GTNAC 2 cut(s) 550, 1011
MalI GATC 2 cut(s) 56, 690
MboI GATC 2 cut(s) 54, 688
MboII GAAGA 7 cut(s) 36, 39, 42, 45, 48, 51, 189
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 8 cut(s) 156, 240, 301, 473, 497, 658, 858, 1000
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 1016
MseI TTAA 3 cut(s) 155, 758, 792
MslI CAYNNNNRTG 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 838
MspI CCGG 2 cut(s) 845, 948
MwoI GCNNNNNNNGC 4 cut(s) 343, 760, 874, 898
NdeI CATATG 1 cut(s) 930
NdeII GATC 2 cut(s) 54, 688
NlaIII CATG 8 cut(s) 109, 170, 205, 337, 353, 732, 778, 905
NlaIV GGNNCC 3 cut(s) 6, 164, 517
NmeAIII GCCGAG 1 cut(s) 537
NmuCI GTSAC 1 cut(s) 1011
NspI RCATGY 2 cut(s) 732, 778
PciI ACATGT 1 cut(s) 774
PfeI GAWTC 1 cut(s) 180
PinAI ACCGGT 1 cut(s) 844
PkrI GCNGC 5 cut(s) 345, 765, 840, 900, 981
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PscI ACATGT 1 cut(s) 774
PspN4I GGNNCC 3 cut(s) 6, 164, 517
PspPI GGNCC 2 cut(s) 318, 950
PsuI RGATCY 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 838
RsaI GTAC 5 cut(s) 365, 455, 609, 727, 913
RsaNI GTAC 5 cut(s) 364, 454, 608, 726, 912
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 3 cut(s) 155, 758, 792
SatI GCNGC 5 cut(s) 344, 764, 839, 899, 980
Sau3AI GATC 2 cut(s) 54, 688
Sau96I GGNCC 2 cut(s) 318, 950
ScaI AGTACT 1 cut(s) 913
SchI GAGTC 1 cut(s) 431
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 5 cut(s) 330, 420, 694, 840, 886
SinI GGWCC 2 cut(s) 318, 950
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 11, 510, 650
SmoI CTYRAG 3 cut(s) 11, 510, 650
Sse9I AATT 8 cut(s) 156, 240, 301, 473, 497, 658, 858, 1000
SspMI CTAG 3 cut(s) 279, 315, 1026
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 4 cut(s) 133, 382, 647, 916
TaqI TCGA 1 cut(s) 490
TasI AATT 8 cut(s) 156, 240, 301, 473, 497, 658, 858, 1000
TatI WGTACW 4 cut(s) 453, 607, 725, 911
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 3 cut(s) 155, 758, 792
Tru9I TTAA 3 cut(s) 155, 758, 792
TscAI CASTG 2 cut(s) 55, 385
TseFI GTSAC 1 cut(s) 1011
TseI GCWGC 5 cut(s) 343, 763, 838, 898, 979
Tsp45I GTSAC 1 cut(s) 1011
TspDTI ATGAA 5 cut(s) 122, 218, 917, 918, 954
TspRI CASTG 2 cut(s) 55, 385
VpaK11BI GGWCC 2 cut(s) 318, 950
XapI RAATTY 5 cut(s) 301, 473, 497, 658, 1000
XceI RCATGY 2 cut(s) 732, 778
XmaJI CCTAGG 1 cut(s) 314
XspI CTAG 3 cut(s) 279, 315, 1026
ZrmI AGTACT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.