Rw3G025480

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017973
Physical Location & Seq
Forward (+)
34093298 .. 34094582
1285 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025480.1

Sequence Viewer

Length: 1137 bp
ATGGTTCCCCTCAAGCAATATAGAAGAAGAAGAAGACCCCCCACTGAGATCAAGGACTTAACCCCTGACATATTGTTTGAAATCTTTGCAAGAGTGCCCATGAAATATCTTTTGCAGTTAACAACAGTTTGCAAATCTTACAAGGCTTTAATTAGGAGCCATGAGTTCAGAAGATTCCATCACGAAAAGAATACCATGAAAAATGCTCCTGATTATCTACTTGTTCGCAATAGAATTGGAGGTCAACAAAGGGGTTTCTCCATTTATTCGGCTAGAACATTTGCCTATGATGAGAATTCAGGGCTTCCTAGGTCCATAGATATTCCCATGCAGATGCAGCTCCATGACCTTCCTCTGTACGAATATGGTTTCACTGTTTATGGTTCTTGCAATGGATTGCTTTGCATCTCTTTATTCTCTCTGGACTTGGACTCTCCTTTATATCTGTATAATCCATCACTCAGAAAATTCAAGCAACTTCCTCGAAGTGAATTTGAGCTTCCTCAAGGCACCTCCATTACTTCTCAGATAGCCACAAGTTTAGTTACTCTCGGCTTTGGGTTCCATTCTGGCATGGATGACTATCAGGTTGTGAGGTTTGTACACCCCAATACAAGATGTACATGCTTGGATGGAGTTATATATTGGCATTTAATGCAGCGTCCTAACATGTCCCTCGTTTCTTTTAATATGCACACTTCAGTTCTTGGAAAGAGGACGCTTCCTTATCAGCTGCAACCGGTGATGCGTCCAATTTGTTTGCAAGTGTTGCATCCAGAGTGTCTGCGAGCGCTGCATGAAAATAGTGTACAAGTGTTGAAGAAATCACTCTGTTTGTTCCAAGTAATACAGCGAGGCTGCCATCACTACTGTGACATATGGGTTTTGAAAGAGGATACTGAGGAAAAAATCGGCAGGATTCGTCTTCCAGCACATGCATCTATAGCATGGCCATTGGGATTTAGCACAAATGGCAATGTTGCGTATATGGTTATCACTGAGGATCTAGACTTACCCCTGTTGGTTCAATATGTTCCTCTGCTGAACCGCATTCAGACTGTTATAACTCAAATAAGTCACAGACCCTGGTACGTTGATGCTTATAGAGAGAGTCTGGTTCTACTTGATGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

378

Amino Acids

43.94

Weight (kDa)

9.0

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 18 - 58 1.4e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017973)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27470
rosa_chinensis RchiOBHm_Chr3g0488541
rosa_laevigata RLG00000022945
rosa_multiflora Rmu_co8412829.1_g000001
rosa_roxburghii Rroxscaffold_6G00394190
rosa_rugosa Rorug03G0239100
rosa_samantha Rh3AG287900 Rh3BG324800 Rh3CG322000 Rh3DG320900
rosa_wichuraiana Rw3G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1064
AccB1I GGYRCC 1 cut(s) 509
AciI CCGC 1 cut(s) 1048
AclWI GGATC 1 cut(s) 1011
AcoI YGGCCR 1 cut(s) 950
AcsI RAATTY 3 cut(s) 295, 467, 491
AcuI CTGAAG 1 cut(s) 684
AfaI GTAC 5 cut(s) 359, 603, 622, 810, 1091
AfeI AGCGCT 1 cut(s) 792
AflIII ACRYGT 1 cut(s) 669
AgeI ACCGGT 1 cut(s) 739
AgsI TTSAA 5 cut(s) 80, 472, 820, 889, 1028
AjnI CCWGG 1 cut(s) 1085
AleI CACNNNNGTG 1 cut(s) 870
AluBI AGCT 3 cut(s) 340, 499, 733
AluI AGCT 3 cut(s) 340, 499, 733
AlwI GGATC 1 cut(s) 1011
AlwNI CAGNNNCTG 1 cut(s) 1086
Aor51HI AGCGCT 1 cut(s) 792
AoxI GGCC 1 cut(s) 950
ApeKI GCWGC 5 cut(s) 337, 658, 733, 793, 858
ApoI RAATTY 3 cut(s) 295, 467, 491
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AsiGI ACCGGT 1 cut(s) 739
AspA2I CCTAGG 1 cut(s) 308
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 754
AvaII GGWCC 1 cut(s) 312
AvrII CCTAGG 1 cut(s) 308
BaeGI GKGCMC 1 cut(s) 99
BalI TGGCCA 1 cut(s) 952
BanI GGYRCC 1 cut(s) 509
BbsI GAAGAC 2 cut(s) 40, 917
BbvI GCAGC 5 cut(s) 349, 670, 720, 780, 845
BccI CCATC 4 cut(s) 186, 463, 626, 870
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 1 cut(s) 1087
BciVI GTATCC 1 cut(s) 889
BfaI CTAG 3 cut(s) 273, 309, 1007
BfmI CTRYAG 1 cut(s) 942
BfoI RGCGCY 1 cut(s) 794
BfuI GTATCC 1 cut(s) 889
BisI GCNGC 5 cut(s) 338, 659, 734, 794, 859
BlnI CCTAGG 1 cut(s) 308
BlsI GCNGC 5 cut(s) 339, 660, 735, 795, 860
Bme1390I CCNGG 1 cut(s) 1087
Bme18I GGWCC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 4 cut(s) 6, 158, 511, 563
BmrFI CCNGG 1 cut(s) 1087
BmsI GCATC 6 cut(s) 324, 414, 735, 781, 947, 1087
BpiI GAAGAC 2 cut(s) 40, 917
BpuEI CTTGAG 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 2 cut(s) 308, 1085
BsaWI WCCGGW 1 cut(s) 739
Bse118I RCCGGY 1 cut(s) 739
Bse3DI GCAATG 2 cut(s) 397, 982
Bse8I GATNNNNATC 1 cut(s) 582
BseBI CCWGG 1 cut(s) 1087
BseDI CCNNGG 2 cut(s) 308, 1085
BseGI GGATG 3 cut(s) 583, 637, 772
BseJI GATNNNNATC 1 cut(s) 582
BseMI GCAATG 2 cut(s) 397, 982
BseMII CTCAG 5 cut(s) 36, 475, 539, 891, 990
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 5 cut(s) 349, 670, 720, 780, 845
BshFI GGCC 1 cut(s) 952
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 739
BsiSI CCGG 1 cut(s) 740
BslFI GGGAC 1 cut(s) 658
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 1 cut(s) 1050
BsnI GGCC 1 cut(s) 952
Bsp1286I GDGCHC 1 cut(s) 99
Bsp1407I TGTACA 3 cut(s) 601, 620, 808
Bsp143I GATC 2 cut(s) 48, 1003
BspACI CCGC 1 cut(s) 1048
BspANI GGCC 1 cut(s) 952
BspCNI CTCAG 5 cut(s) 37, 474, 538, 892, 991
BspLI GGNNCC 4 cut(s) 6, 158, 511, 563
BspPI GGATC 1 cut(s) 1011
BspT107I GGYRCC 1 cut(s) 509
BsrDI GCAATG 2 cut(s) 397, 982
BsrFI RCCGGY 1 cut(s) 739
BsrGI TGTACA 3 cut(s) 601, 620, 808
BssAI RCCGGY 1 cut(s) 739
BssECI CCNNGG 2 cut(s) 308, 1085
BssMI GATC 2 cut(s) 48, 1003
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 1087
Bst4CI ACNGT 4 cut(s) 127, 376, 872, 1060
BstAPI GCANNNNNTGC 2 cut(s) 655, 769
BstAUI TGTACA 3 cut(s) 601, 620, 808
BstC8I GCNNGC 1 cut(s) 789
BstDEI CTNAG 5 cut(s) 45, 461, 525, 900, 999
BstF5I GGATG 3 cut(s) 583, 637, 772
BstH2I RGCGCY 1 cut(s) 794
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 2 cut(s) 51, 1006
BstMBI GATC 2 cut(s) 48, 1003
BstMWI GCNNNNNNNGC 6 cut(s) 337, 655, 769, 793, 944, 972
BstNI CCWGG 1 cut(s) 1087
BstNSI RCATGY 3 cut(s) 627, 673, 938
BstSCI CCNGG 1 cut(s) 1085
BstSFI CTRYAG 1 cut(s) 942
BstSLI GKGCMC 1 cut(s) 99
BstV1I GCAGC 5 cut(s) 349, 670, 720, 780, 845
BstV2I GAAGAC 2 cut(s) 40, 917
BstX2I RGATCY 1 cut(s) 1003
BstYI RGATCY 1 cut(s) 1003
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 1 cut(s) 952
BtsCI GGATG 3 cut(s) 583, 637, 772
BtsIMutI CAGTG 3 cut(s) 42, 372, 996
Cac8I GCNNGC 1 cut(s) 789
CaiI CAGNNNCTG 1 cut(s) 1086
CfoI GCGC 1 cut(s) 793
Cfr10I RCCGGY 1 cut(s) 739
Cfr13I GGNCC 1 cut(s) 312
CseI GACGC 3 cut(s) 650, 727, 737
Csp6I GTAC 5 cut(s) 358, 602, 621, 809, 1090
CspAI ACCGGT 1 cut(s) 739
CviQI GTAC 5 cut(s) 358, 602, 621, 809, 1090
DdeI CTNAG 5 cut(s) 45, 461, 525, 900, 999
DpnI GATC 2 cut(s) 50, 1005
DpnII GATC 2 cut(s) 48, 1003
EaeI YGGCCR 1 cut(s) 950
Eco130I CCWWGG 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 312
Eco47III AGCGCT 1 cut(s) 792
Eco57I CTGAAG 1 cut(s) 684
EcoRI GAATTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 1085
EcoT14I CCWWGG 1 cut(s) 308
EcoT22I ATGCAT 1 cut(s) 940
ErhI CCWWGG 1 cut(s) 308
FaqI GGGAC 1 cut(s) 658
FauNDI CATATG 1 cut(s) 878
Fnu4HI GCNGC 5 cut(s) 338, 659, 734, 794, 859
FokI GGATG 3 cut(s) 590, 644, 759
Fsp4HI GCNGC 5 cut(s) 338, 659, 734, 794, 859
FspBI CTAG 3 cut(s) 273, 309, 1007
GlaI GCGC 1 cut(s) 792
GluI GCNGC 5 cut(s) 338, 659, 734, 794, 859
HaeII RGCGCY 1 cut(s) 794
HaeIII GGCC 1 cut(s) 952
HapII CCGG 1 cut(s) 740
HgaI GACGC 3 cut(s) 650, 727, 737
HhaI GCGC 1 cut(s) 793
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 2 cut(s) 120, 245
HindII GTYRAC 2 cut(s) 120, 245
HinfI GANTC 4 cut(s) 174, 431, 919, 1111
HpaI GTTAAC 1 cut(s) 120
HpaII CCGG 1 cut(s) 740
HphI GGTGA 1 cut(s) 754
Hpy166II GTNNAC 4 cut(s) 120, 245, 604, 809
Hpy188I TCNGA 5 cut(s) 170, 464, 528, 1056, 1136
Hpy188III TCNNGA 5 cut(s) 182, 209, 422, 776, 1007
Hpy8I GTNNAC 4 cut(s) 120, 245, 604, 809
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 4 cut(s) 127, 376, 872, 1060
HpyCH4IV ACGT 1 cut(s) 1092
HpyF10VI GCNNNNNNNGC 6 cut(s) 337, 655, 769, 793, 944, 972
HpyF3I CTNAG 5 cut(s) 45, 461, 525, 900, 999
HpySE526I ACGT 1 cut(s) 1092
HspAI GCGC 1 cut(s) 791
KspAI GTTAAC 1 cut(s) 120
Kzo9I GATC 2 cut(s) 48, 1003
LmnI GCTCC 3 cut(s) 156, 211, 345
Lsp1109I GCAGC 5 cut(s) 349, 670, 720, 780, 845
LweI GCATC 6 cut(s) 324, 414, 735, 781, 947, 1087
MaeI CTAG 3 cut(s) 273, 309, 1007
MaeII ACGT 1 cut(s) 1092
MaeIII GTNAC 3 cut(s) 544, 872, 1076
MalI GATC 2 cut(s) 50, 1005
MboI GATC 2 cut(s) 48, 1003
MboII GAAGA 7 cut(s) 36, 39, 42, 45, 183, 832, 917
MflI RGATCY 1 cut(s) 1003
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 1 cut(s) 952
MluCI AATT 6 cut(s) 150, 234, 295, 467, 491, 753
MluNI TGGCCA 1 cut(s) 952
MlyI GAGTC 2 cut(s) 425, 1120
Mox20I TGGCCA 1 cut(s) 952
Mph1103I ATGCAT 1 cut(s) 940
MscI TGGCCA 1 cut(s) 952
MseI TTAA 5 cut(s) 59, 119, 149, 653, 687
MslI CAYNNNNRTG 2 cut(s) 332, 870
Msp20I TGGCCA 1 cut(s) 952
MspA1I CMGCKG 1 cut(s) 733
MspI CCGG 1 cut(s) 740
MspR9I CCNGG 1 cut(s) 1087
Mva1269I GAATGC 1 cut(s) 1050
MvaI CCWGG 1 cut(s) 1087
MwoI GCNNNNNNNGC 6 cut(s) 337, 655, 769, 793, 944, 972
NdeI CATATG 1 cut(s) 878
NdeII GATC 2 cut(s) 48, 1003
NlaIV GGNNCC 4 cut(s) 6, 158, 511, 563
NmeAIII GCCGAG 1 cut(s) 531
NmuCI GTSAC 2 cut(s) 872, 1076
NsiI ATGCAT 1 cut(s) 940
NspI RCATGY 3 cut(s) 627, 673, 938
OliI CACNNNNGTG 1 cut(s) 870
PciI ACATGT 1 cut(s) 669
PctI GAATGC 1 cut(s) 1050
PfeI GAWTC 2 cut(s) 174, 919
PinAI ACCGGT 1 cut(s) 739
PkrI GCNGC 5 cut(s) 339, 660, 735, 795, 860
PleI GAGTC 2 cut(s) 425, 1119
PpsI GAGTC 2 cut(s) 425, 1119
PscI ACATGT 1 cut(s) 669
PsiI TTATAA 1 cut(s) 1064
Psp6I CCWGG 1 cut(s) 1085
PspGI CCWGG 1 cut(s) 1085
PspN4I GGNNCC 4 cut(s) 6, 158, 511, 563
PspPI GGNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 1086
PsuI RGATCY 1 cut(s) 1003
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 5 cut(s) 359, 603, 622, 810, 1091
RsaNI GTAC 5 cut(s) 358, 602, 621, 809, 1090
RseI CAYNNNNRTG 2 cut(s) 332, 870
SaqAI TTAA 5 cut(s) 59, 119, 149, 653, 687
SatI GCNGC 5 cut(s) 338, 659, 734, 794, 859
Sau3AI GATC 2 cut(s) 48, 1003
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 2 cut(s) 425, 1120
ScrFI CCNGG 1 cut(s) 1087
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 6 cut(s) 324, 414, 735, 781, 947, 1087
SfcI CTRYAG 1 cut(s) 942
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 332, 870
SmlI CTYRAG 2 cut(s) 11, 504
SmoI CTYRAG 2 cut(s) 11, 504
Sse9I AATT 6 cut(s) 150, 234, 295, 467, 491, 753
SsiI CCGC 1 cut(s) 1048
SspMI CTAG 3 cut(s) 273, 309, 1007
StyD4I CCNGG 1 cut(s) 1085
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 4 cut(s) 127, 376, 872, 1060
TaiI ACGT 1 cut(s) 1095
TaqI TCGA 1 cut(s) 484
TasI AATT 6 cut(s) 150, 234, 295, 467, 491, 753
TatI WGTACW 3 cut(s) 601, 620, 808
TfiI GAWTC 2 cut(s) 174, 919
Tru1I TTAA 5 cut(s) 59, 119, 149, 653, 687
Tru9I TTAA 5 cut(s) 59, 119, 149, 653, 687
TscAI CASTG 3 cut(s) 49, 379, 1003
TseFI GTSAC 2 cut(s) 872, 1076
TseI GCWGC 5 cut(s) 337, 658, 733, 793, 858
Tsp45I GTSAC 2 cut(s) 872, 1076
TspDTI ATGAA 3 cut(s) 116, 212, 813
TspRI CASTG 3 cut(s) 49, 379, 1003
VpaK11BI GGWCC 1 cut(s) 312
XapI RAATTY 3 cut(s) 295, 467, 491
XbaI TCTAGA 1 cut(s) 1006
XceI RCATGY 3 cut(s) 627, 673, 938
XmaJI CCTAGG 1 cut(s) 308
XspI CTAG 3 cut(s) 273, 309, 1007
Zsp2I ATGCAT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.