Rh3BG324800

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0017973
Physical Location & Seq
Forward (+)
33728104 .. 33729342
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG324800.1

Sequence Viewer

Length: 1239 bp
ATGGTTCCCCTCAAGCAATATAGAAGAAGAAGAAGACCCCCCACTGAGATCAAAGACTTGACCCCTGACATATTGTTTGAAATCTTTGCAAGAGTGCCCATGAAATATCTTTTGCAGTTAACAACAGTTTGCAAATCTTACAAGGCTTTAATTAGGGGCCATGAGTTCAGAAGATTCCATCACGAAAAGAATACCATGAAAAATGCGCCTGATTATCTACTTGTTCGCAATAGAATTGGAGGTCAACAAAGGGGCTTCTCCATTTATTCTGCTAGAACATTTGCCTATGATGAGAATTCAGGGCTTCCTAGGTCCATAGATATTCCCATGCAGATGCAGCTCCATGACCTTCCTCTGTACGAATATGGTTTCACTGTTTATGGTTCTTGCAATGGATTGCTTTGCATCTCTTTATTCTCTCTGGACTTGGACTCTCCTTTATACCTGTACAATCCATCACTCAGAAAATTCAAGCAACTTCCTCGAAGTGAATTTGAGCTTCCTCAAGGCACCTCCATTACTTCTCAGATAGCCACAGGTTTAGTTACTCTCGGCTTTGGGTTCCATTCTGGGAAGGATGACTATCAGGTTGTGAGGTTTGTACACCCCAATACAAGTTTCTTTCATGCTGAGGTTTACAGTCTCAAGTTGAATTCTTGGACACCAGTAGAGAATGTTGTAGATCCCCGTGAGGTTGATGCTTGGTTTATGGAAGGATGTACATGCTTGGATGGAGTTATATATTGGCATTTAATGCAGCGTCCTAACATGTCCCTCATTTCTTTTAATATGCACACTTCAGTTCTTGGAAAGAGGACGCTTCCTTATGATCAGCTGCAACCGGTGATGCGTCCAATTTGTTTGCAAGTGTTGCATCCAGAGTGTTTGCGAGCGCTGCATGAAAATAGTGTGCAAGTGTCGAAGAAATCACTCTGTTTGTTCCAAGTAATACAGCGAGGCGGCCATCACTACTGTGACATATGGGTTCTGAAAGAGGATACTGAGGAAAAAATCGGCAGGATTCATCTTCCACCACATGCATCTATAGCATGGCCATTGGGATTTAGCACAAATGGCAATGTTGCTTATATGGTTATCACTGAGAATCAAGACTTACCCCTGTTGGTTCAATATGTTCCTCTGCTGAACCGCATTCAGACTGTTATAACTCAAATAAGTCACAGACCGTGGTACGTTGATGCTTATAGAGAGAGTCTGGTTCTACTCGATGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

412

Amino Acids

47.78

Weight (kDa)

8.47

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 18 - 58 8.5e-07 F-box domain
FBA_1 PF07734 102 - 304 5.9e-14 F-box associated beta propeller domain
FBA_3 PF08268 122 - 266 2.5e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017973)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27470
rosa_chinensis RchiOBHm_Chr3g0488541
rosa_laevigata RLG00000022945
rosa_multiflora Rmu_co8412829.1_g000001
rosa_roxburghii Rroxscaffold_6G00394190
rosa_rugosa Rorug03G0239100
rosa_samantha Rh3AG287900 Rh3BG324800 Rh3CG322000 Rh3DG320900
rosa_wichuraiana Rw3G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1166
AccB1I GGYRCC 1 cut(s) 509
AciI CCGC 2 cut(s) 960, 1150
AclWI GGATC 1 cut(s) 677
AcoI YGGCCR 2 cut(s) 961, 1052
AcsI RAATTY 4 cut(s) 295, 467, 491, 652
AcuI CTGAAG 1 cut(s) 783
AfaI GTAC 5 cut(s) 359, 449, 603, 721, 1193
AfeI AGCGCT 1 cut(s) 894
AflIII ACRYGT 1 cut(s) 768
AgeI ACCGGT 1 cut(s) 841
AgsI TTSAA 4 cut(s) 80, 472, 652, 1130
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AleI CACNNNNGTG 1 cut(s) 972
AluBI AGCT 3 cut(s) 340, 499, 835
AluI AGCT 3 cut(s) 340, 499, 835
Alw26I GTCTC 1 cut(s) 647
AlwI GGATC 1 cut(s) 677
Aor51HI AGCGCT 1 cut(s) 894
AoxI GGCC 3 cut(s) 157, 961, 1052
ApeKI GCWGC 4 cut(s) 337, 757, 835, 895
ApoI RAATTY 4 cut(s) 295, 467, 491, 652
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AsiGI ACCGGT 1 cut(s) 841
AspA2I CCTAGG 1 cut(s) 308
AspLEI GCGC 2 cut(s) 208, 895
AspS9I GGNCC 2 cut(s) 157, 312
AsuHPI GGTGA 1 cut(s) 856
AvaII GGWCC 1 cut(s) 312
AvrII CCTAGG 1 cut(s) 308
BaeGI GKGCMC 1 cut(s) 99
BalI TGGCCA 1 cut(s) 1054
BanI GGYRCC 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 40
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 4 cut(s) 349, 769, 822, 882
BccI CCATC 4 cut(s) 186, 463, 725, 972
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciVI GTATCC 1 cut(s) 991
BclI TGATCA 1 cut(s) 829
BcoDI GTCTC 1 cut(s) 647
BfaI CTAG 2 cut(s) 273, 309
BfmI CTRYAG 1 cut(s) 1044
BfoI RGCGCY 1 cut(s) 896
BfuI GTATCC 1 cut(s) 991
BisI GCNGC 5 cut(s) 338, 758, 836, 896, 961
BlnI CCTAGG 1 cut(s) 308
BlsI GCNGC 5 cut(s) 339, 759, 837, 897, 962
Bme18I GGWCC 1 cut(s) 312
BmgT120I GGNCC 2 cut(s) 157, 312
BmiI GGNNCC 4 cut(s) 6, 158, 511, 563
BmsI GCATC 7 cut(s) 324, 414, 688, 837, 883, 1049, 1189
BpiI GAAGAC 1 cut(s) 40
Bpu10I CCTNAGC 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 489, 629
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 2 cut(s) 308, 1187
BsaWI WCCGGW 1 cut(s) 841
Bse118I RCCGGY 1 cut(s) 841
Bse1I ACTGG 1 cut(s) 665
Bse3DI GCAATG 2 cut(s) 397, 1084
Bse8I GATNNNNATC 1 cut(s) 582
BseDI CCNNGG 2 cut(s) 308, 1187
BseGI GGATG 4 cut(s) 583, 722, 736, 874
BseJI GATNNNNATC 1 cut(s) 582
BseMI GCAATG 2 cut(s) 397, 1084
BseMII CTCAG 6 cut(s) 36, 475, 539, 621, 993, 1092
BseNI ACTGG 1 cut(s) 665
BseSI GKGCMC 1 cut(s) 99
BseXI GCAGC 4 cut(s) 349, 769, 822, 882
BshFI GGCC 3 cut(s) 159, 963, 1054
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 841
BsiSI CCGG 1 cut(s) 842
BslFI GGGAC 1 cut(s) 757
BsmAI GTCTC 1 cut(s) 647
BsmFI GGGAC 1 cut(s) 757
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 3 cut(s) 159, 963, 1054
Bsp1286I GDGCHC 1 cut(s) 99
Bsp1407I TGTACA 3 cut(s) 447, 601, 719
Bsp143I GATC 3 cut(s) 48, 682, 829
BspACI CCGC 2 cut(s) 960, 1150
BspANI GGCC 3 cut(s) 159, 963, 1054
BspCNI CTCAG 6 cut(s) 37, 474, 538, 622, 994, 1093
BspLI GGNNCC 4 cut(s) 6, 158, 511, 563
BspPI GGATC 1 cut(s) 677
BspT107I GGYRCC 1 cut(s) 509
BsrDI GCAATG 2 cut(s) 397, 1084
BsrFI RCCGGY 1 cut(s) 841
BsrGI TGTACA 3 cut(s) 447, 601, 719
BsrI ACTGG 1 cut(s) 665
BssAI RCCGGY 1 cut(s) 841
BssECI CCNNGG 2 cut(s) 308, 1187
BssMI GATC 3 cut(s) 48, 682, 829
BssT1I CCWWGG 1 cut(s) 308
Bst4CI ACNGT 6 cut(s) 127, 376, 641, 974, 1162, 1188
BstAPI GCANNNNNTGC 2 cut(s) 754, 871
BstAUI TGTACA 3 cut(s) 447, 601, 719
BstC8I GCNNGC 1 cut(s) 891
BstDEI CTNAG 6 cut(s) 45, 461, 525, 630, 1002, 1101
BstDSI CCRYGG 1 cut(s) 1187
BstF5I GGATG 4 cut(s) 583, 722, 736, 874
BstH2I RGCGCY 1 cut(s) 896
BstHHI GCGC 2 cut(s) 208, 895
BstKTI GATC 3 cut(s) 51, 685, 832
BstMAI GTCTC 1 cut(s) 647
BstMBI GATC 3 cut(s) 48, 682, 829
BstMWI GCNNNNNNNGC 6 cut(s) 337, 754, 871, 895, 1046, 1074
BstNSI RCATGY 3 cut(s) 726, 772, 1040
BstSFI CTRYAG 1 cut(s) 1044
BstSLI GKGCMC 1 cut(s) 99
BstV1I GCAGC 4 cut(s) 349, 769, 822, 882
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 682
BstYI RGATCY 1 cut(s) 682
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 3 cut(s) 159, 963, 1054
BtgI CCRYGG 1 cut(s) 1187
BtsCI GGATG 4 cut(s) 583, 722, 736, 874
BtsIMutI CAGTG 3 cut(s) 42, 372, 1098
Cac8I GCNNGC 1 cut(s) 891
CfoI GCGC 2 cut(s) 208, 895
Cfr10I RCCGGY 1 cut(s) 841
Cfr13I GGNCC 2 cut(s) 157, 312
CseI GACGC 3 cut(s) 749, 826, 839
Csp6I GTAC 5 cut(s) 358, 448, 602, 720, 1192
CspAI ACCGGT 1 cut(s) 841
CviQI GTAC 5 cut(s) 358, 448, 602, 720, 1192
DdeI CTNAG 6 cut(s) 45, 461, 525, 630, 1002, 1101
DpnI GATC 3 cut(s) 50, 684, 831
DpnII GATC 3 cut(s) 48, 682, 829
EaeI YGGCCR 2 cut(s) 961, 1052
Eco130I CCWWGG 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 312
Eco47III AGCGCT 1 cut(s) 894
Eco57I CTGAAG 1 cut(s) 783
EcoRI GAATTC 2 cut(s) 295, 652
EcoT14I CCWWGG 1 cut(s) 308
EcoT22I ATGCAT 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 308
FaqI GGGAC 1 cut(s) 757
FauNDI CATATG 1 cut(s) 980
FbaI TGATCA 1 cut(s) 829
Fnu4HI GCNGC 5 cut(s) 338, 758, 836, 896, 961
FokI GGATG 4 cut(s) 590, 729, 743, 861
Fsp4HI GCNGC 5 cut(s) 338, 758, 836, 896, 961
FspBI CTAG 2 cut(s) 273, 309
GlaI GCGC 2 cut(s) 207, 894
GluI GCNGC 5 cut(s) 338, 758, 836, 896, 961
HaeII RGCGCY 1 cut(s) 896
HaeIII GGCC 3 cut(s) 159, 963, 1054
HapII CCGG 1 cut(s) 842
HgaI GACGC 3 cut(s) 749, 826, 839
HhaI GCGC 2 cut(s) 208, 895
Hin6I GCGC 2 cut(s) 206, 893
HinP1I GCGC 2 cut(s) 206, 893
HincII GTYRAC 2 cut(s) 120, 245
HindII GTYRAC 2 cut(s) 120, 245
HinfI GANTC 5 cut(s) 174, 431, 1021, 1105, 1213
HpaI GTTAAC 1 cut(s) 120
HpaII CCGG 1 cut(s) 842
HphI GGTGA 1 cut(s) 856
Hpy166II GTNNAC 4 cut(s) 120, 245, 604, 637
Hpy188I TCNGA 6 cut(s) 170, 464, 528, 990, 1158, 1238
Hpy188III TCNNGA 4 cut(s) 182, 422, 878, 1109
Hpy8I GTNNAC 4 cut(s) 120, 245, 604, 637
HpyAV CCTTC 3 cut(s) 359, 568, 707
HpyCH4III ACNGT 6 cut(s) 127, 376, 641, 974, 1162, 1188
HpyCH4IV ACGT 1 cut(s) 1194
HpyF10VI GCNNNNNNNGC 6 cut(s) 337, 754, 871, 895, 1046, 1074
HpyF3I CTNAG 6 cut(s) 45, 461, 525, 630, 1002, 1101
HpySE526I ACGT 1 cut(s) 1194
HspAI GCGC 2 cut(s) 206, 893
Ksp22I TGATCA 1 cut(s) 829
KspAI GTTAAC 1 cut(s) 120
Kzo9I GATC 3 cut(s) 48, 682, 829
LmnI GCTCC 1 cut(s) 345
Lsp1109I GCAGC 4 cut(s) 349, 769, 822, 882
LweI GCATC 7 cut(s) 324, 414, 688, 837, 883, 1049, 1189
MaeI CTAG 2 cut(s) 273, 309
MaeII ACGT 1 cut(s) 1194
MaeIII GTNAC 3 cut(s) 544, 974, 1178
MalI GATC 3 cut(s) 50, 684, 831
MboI GATC 3 cut(s) 48, 682, 829
MboII GAAGA 7 cut(s) 36, 39, 42, 45, 183, 934, 1019
MflI RGATCY 1 cut(s) 682
MhlI GDGCHC 1 cut(s) 99
MlsI TGGCCA 1 cut(s) 1054
MluCI AATT 7 cut(s) 150, 234, 295, 467, 491, 652, 855
MluNI TGGCCA 1 cut(s) 1054
MlyI GAGTC 2 cut(s) 425, 1222
Mox20I TGGCCA 1 cut(s) 1054
Mph1103I ATGCAT 1 cut(s) 1042
MscI TGGCCA 1 cut(s) 1054
MseI TTAA 4 cut(s) 119, 149, 752, 786
MslI CAYNNNNRTG 2 cut(s) 332, 972
Msp20I TGGCCA 1 cut(s) 1054
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 1 cut(s) 842
Mva1269I GAATGC 1 cut(s) 1152
MwoI GCNNNNNNNGC 6 cut(s) 337, 754, 871, 895, 1046, 1074
NdeI CATATG 1 cut(s) 980
NdeII GATC 3 cut(s) 48, 682, 829
NlaIV GGNNCC 4 cut(s) 6, 158, 511, 563
NmeAIII GCCGAG 1 cut(s) 531
NmuCI GTSAC 2 cut(s) 974, 1178
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 3 cut(s) 726, 772, 1040
OliI CACNNNNGTG 1 cut(s) 972
PciI ACATGT 1 cut(s) 768
PctI GAATGC 1 cut(s) 1152
PfeI GAWTC 3 cut(s) 174, 1021, 1105
PinAI ACCGGT 1 cut(s) 841
PkrI GCNGC 5 cut(s) 339, 759, 837, 897, 962
PleI GAGTC 2 cut(s) 425, 1221
PpsI GAGTC 2 cut(s) 425, 1221
PscI ACATGT 1 cut(s) 768
PsiI TTATAA 1 cut(s) 1166
PspN4I GGNNCC 4 cut(s) 6, 158, 511, 563
PspPI GGNCC 2 cut(s) 157, 312
PsuI RGATCY 1 cut(s) 682
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 5 cut(s) 359, 449, 603, 721, 1193
RsaNI GTAC 5 cut(s) 358, 448, 602, 720, 1192
RseI CAYNNNNRTG 2 cut(s) 332, 972
SaqAI TTAA 4 cut(s) 119, 149, 752, 786
SatI GCNGC 5 cut(s) 338, 758, 836, 896, 961
Sau3AI GATC 3 cut(s) 48, 682, 829
Sau96I GGNCC 2 cut(s) 157, 312
SchI GAGTC 2 cut(s) 425, 1222
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 7 cut(s) 324, 414, 688, 837, 883, 1049, 1189
SfcI CTRYAG 1 cut(s) 1044
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 332, 972
SmlI CTYRAG 3 cut(s) 11, 504, 644
SmoI CTYRAG 3 cut(s) 11, 504, 644
Sse9I AATT 7 cut(s) 150, 234, 295, 467, 491, 652, 855
SsiI CCGC 2 cut(s) 960, 1150
SspMI CTAG 2 cut(s) 273, 309
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 6 cut(s) 127, 376, 641, 974, 1162, 1188
TaiI ACGT 1 cut(s) 1197
TaqI TCGA 3 cut(s) 484, 920, 1227
TasI AATT 7 cut(s) 150, 234, 295, 467, 491, 652, 855
TatI WGTACW 3 cut(s) 447, 601, 719
TauI GCSGC 1 cut(s) 963
TfiI GAWTC 3 cut(s) 174, 1021, 1105
Tru1I TTAA 4 cut(s) 119, 149, 752, 786
Tru9I TTAA 4 cut(s) 119, 149, 752, 786
TscAI CASTG 3 cut(s) 49, 379, 1105
TseFI GTSAC 2 cut(s) 974, 1178
TseI GCWGC 4 cut(s) 337, 757, 835, 895
Tsp45I GTSAC 2 cut(s) 974, 1178
TspDTI ATGAA 5 cut(s) 116, 212, 614, 915, 1013
TspRI CASTG 3 cut(s) 49, 379, 1105
VpaK11BI GGWCC 1 cut(s) 312
XapI RAATTY 4 cut(s) 295, 467, 491, 652
XceI RCATGY 3 cut(s) 726, 772, 1040
XmaJI CCTAGG 1 cut(s) 308
XspI CTAG 2 cut(s) 273, 309
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.