Rroxscaffold_6G00394190

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017973
Physical Location & Seq
Reverse (-)
14538248 .. 14541724
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394190.1

Sequence Viewer

Length: 1326 bp
ATGATAGTTTCTTGGAGGAAATATATAGCTTTTTCTCTTGAAAAGAGGGAACTTCAGTACAAGGGAGGAACCAAGAATACAAACGAGCAAGAGGTGATGCTTCTTAAACTTAAGAAGAAGAAGACCCCCACTGAGATCAAAGACTTAACCCCTGACATATTGTTTGAAATCTTTGCAAGAGTGCCCATGAAATATCTTTTGCAGTTAACAACAGTTTGCAAATCTTACAAGGCTTTAATTAGGAGCCATGAGTTCAGAAGATTCCATCACGAAAAGAATACCATGAAAAATGCTCCTGATTCTCTACTTGTTCGCAATAGAATTGGAGGTCAACAAAGGGGCTTCTCCATTTATTCTGCTAGAACATTTGCCTATGATGAGAATTCAGGGCTTCCTAGGTCCATAGATATTCCCATGCAGATGCAGCTCCATGGCCTTCCTCTGTACGAATATGGTTTCACTGTTTATGGTTCTTGCAATGGATTGCTTTGCATCTCTTTATTCTCTCTGGACTTGGACTCTCCTTTATATCTGTACAATCCATCACTCCGAAAATTCAAGCAACTTCCTCCAAGTGAATTTGAGCTTCCTCAAGGCACCTCCACTACTTCTCAGATAGCCACAGGTTTAGTTACTCTCGGCTTTGGGTTCCATTCTGGGAAGGATGACTATCAGGTTGTGAGGTTTGTACACCCCAATACAAGTATCTTTTATGCTGAGGTTTACAGTCTCAAGTTGAATTCTTGGACACCAGTAGAGAATGTTGTAGATCCCCGTGAGGTTGATGCTTGGTTTATGGAAGGATGTACATGCTTGGATGGAGTTATATATTGGCATTTAATGCAGCGTCCTAACATGTCCCTCGTTTCTTTTAATATGCACACTTCAGTTCTTGGAAAGAGGACGCTTCCTTATCATCAGCTGCAACCGGTGATGCGTCCAATTTGTTTGCAAGTGTTGCATCCAGAGTGTTTGCGAGCGCTGCATGAAAATAGTGTGCAAGTGTCGAAGAAATCACTCTGTTTGTTCCAAGTAATACAGCGAGGCTGCCATCACTACTGTGACATATGGGTTCTGAAAGAGGATACTGAGGAAAAAATCGGCAGGATTCGTCTTCCACCACATGCATCTATAGCATGGCCATTGGGATTTAGCACAAATGGCAATGTTGCTTATATGGTTATCACTGAGAATCAAGACTTACCCCTGTTGGTTCAATATGTTCCTCTGCTGAACCGCATTCAGACTGTTATAACTCAAATAAGTCACAGACCCTGGTACGTTGATGCTTATAGAGAGAGTCTGGTTCTACTCGATGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

441

Amino Acids

50.97

Weight (kDa)

8.75

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 47 - 87 1.7e-07 F-box domain
FBA_3 PF08268 151 - 295 2.1e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017973)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27470
rosa_chinensis RchiOBHm_Chr3g0488541
rosa_laevigata RLG00000022945
rosa_multiflora Rmu_co8412829.1_g000001
rosa_roxburghii Rroxscaffold_6G00394190
rosa_rugosa Rorug03G0239100
rosa_samantha Rh3AG287900 Rh3BG324800 Rh3CG322000 Rh3DG320900
rosa_wichuraiana Rw3G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1253
AccB1I GGYRCC 1 cut(s) 596
AciI CCGC 1 cut(s) 1237
AclWI GGATC 1 cut(s) 764
AcoI YGGCCR 1 cut(s) 1139
AcsI RAATTY 4 cut(s) 382, 554, 578, 739
AcuI CTGAAG 2 cut(s) 38, 870
AfaI GTAC 6 cut(s) 59, 446, 536, 690, 808, 1280
AfeI AGCGCT 1 cut(s) 981
AflII CTTAAG 1 cut(s) 110
AflIII ACRYGT 1 cut(s) 855
AgeI ACCGGT 1 cut(s) 928
AgsI TTSAA 5 cut(s) 41, 167, 559, 739, 1217
AjnI CCWGG 1 cut(s) 1274
AleI CACNNNNGTG 1 cut(s) 1059
AluBI AGCT 4 cut(s) 29, 427, 586, 922
AluI AGCT 4 cut(s) 29, 427, 586, 922
Alw26I GTCTC 1 cut(s) 734
AlwI GGATC 1 cut(s) 764
AlwNI CAGNNNCTG 1 cut(s) 1275
Aor51HI AGCGCT 1 cut(s) 981
AoxI GGCC 2 cut(s) 433, 1139
ApeKI GCWGC 5 cut(s) 424, 844, 922, 982, 1047
ApoI RAATTY 4 cut(s) 382, 554, 578, 739
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AsiGI ACCGGT 1 cut(s) 928
AspA2I CCTAGG 1 cut(s) 395
AspLEI GCGC 1 cut(s) 982
AspS9I GGNCC 1 cut(s) 399
AsuHPI GGTGA 2 cut(s) 106, 943
AvaII GGWCC 1 cut(s) 399
AvrII CCTAGG 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 186
BalI TGGCCA 1 cut(s) 1141
BanI GGYRCC 1 cut(s) 596
BbsI GAAGAC 2 cut(s) 128, 1106
BbvCI CCTCAGC 1 cut(s) 717
BbvI GCAGC 5 cut(s) 436, 856, 909, 969, 1034
BccI CCATC 4 cut(s) 273, 550, 812, 1059
BciT130I CCWGG 1 cut(s) 1276
BciVI GTATCC 1 cut(s) 1078
BcoDI GTCTC 1 cut(s) 734
BfaI CTAG 2 cut(s) 360, 396
BfmI CTRYAG 1 cut(s) 1131
BfoI RGCGCY 1 cut(s) 983
BfrI CTTAAG 1 cut(s) 110
BfuI GTATCC 1 cut(s) 1078
BisI GCNGC 5 cut(s) 425, 845, 923, 983, 1048
BlnI CCTAGG 1 cut(s) 395
BlsI GCNGC 5 cut(s) 426, 846, 924, 984, 1049
Bme1390I CCNGG 1 cut(s) 1276
Bme18I GGWCC 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 4 cut(s) 70, 245, 598, 650
BmrFI CCNGG 1 cut(s) 1276
BmsI GCATC 8 cut(s) 87, 411, 501, 775, 924, 970, 1136, 1276
BpiI GAAGAC 2 cut(s) 128, 1106
Bpu10I CCTNAGC 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 576, 716
BsaBI GATNNNNATC 1 cut(s) 669
BsaJI CCNNGG 3 cut(s) 395, 430, 1274
BsaWI WCCGGW 1 cut(s) 928
Bse118I RCCGGY 1 cut(s) 928
Bse1I ACTGG 1 cut(s) 752
Bse3DI GCAATG 2 cut(s) 484, 1171
Bse8I GATNNNNATC 1 cut(s) 669
BseBI CCWGG 1 cut(s) 1276
BseDI CCNNGG 3 cut(s) 395, 430, 1274
BseGI GGATG 4 cut(s) 670, 809, 823, 961
BseJI GATNNNNATC 1 cut(s) 669
BseMI GCAATG 2 cut(s) 484, 1171
BseMII CTCAG 5 cut(s) 123, 626, 708, 1080, 1179
BseNI ACTGG 1 cut(s) 752
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 5 cut(s) 436, 856, 909, 969, 1034
BshFI GGCC 2 cut(s) 435, 1141
BshNI GGYRCC 1 cut(s) 596
BshTI ACCGGT 1 cut(s) 928
BsiSI CCGG 1 cut(s) 929
BslFI GGGAC 1 cut(s) 844
BsmAI GTCTC 1 cut(s) 734
BsmFI GGGAC 1 cut(s) 844
BsmI GAATGC 1 cut(s) 1239
BsnI GGCC 2 cut(s) 435, 1141
Bsp1286I GDGCHC 1 cut(s) 186
Bsp1407I TGTACA 3 cut(s) 534, 688, 806
Bsp143I GATC 2 cut(s) 135, 769
Bsp19I CCATGG 1 cut(s) 430
BspACI CCGC 1 cut(s) 1237
BspANI GGCC 2 cut(s) 435, 1141
BspCNI CTCAG 5 cut(s) 124, 625, 709, 1081, 1180
BspLI GGNNCC 4 cut(s) 70, 245, 598, 650
BspPI GGATC 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 596
BspTI CTTAAG 1 cut(s) 110
BsrDI GCAATG 2 cut(s) 484, 1171
BsrFI RCCGGY 1 cut(s) 928
BsrGI TGTACA 3 cut(s) 534, 688, 806
BsrI ACTGG 1 cut(s) 752
BssAI RCCGGY 1 cut(s) 928
BssECI CCNNGG 3 cut(s) 395, 430, 1274
BssMI GATC 2 cut(s) 135, 769
BssT1I CCWWGG 2 cut(s) 395, 430
Bst2UI CCWGG 1 cut(s) 1276
Bst4CI ACNGT 5 cut(s) 214, 463, 728, 1061, 1249
BstAFI CTTAAG 1 cut(s) 110
BstAPI GCANNNNNTGC 2 cut(s) 841, 958
BstAUI TGTACA 3 cut(s) 534, 688, 806
BstC8I GCNNGC 1 cut(s) 978
BstDEI CTNAG 5 cut(s) 132, 612, 717, 1089, 1188
BstDSI CCRYGG 1 cut(s) 430
BstF5I GGATG 4 cut(s) 670, 809, 823, 961
BstH2I RGCGCY 1 cut(s) 983
BstHHI GCGC 1 cut(s) 982
BstKTI GATC 2 cut(s) 138, 772
BstMAI GTCTC 1 cut(s) 734
BstMBI GATC 2 cut(s) 135, 769
BstMWI GCNNNNNNNGC 6 cut(s) 424, 841, 958, 982, 1133, 1161
BstNI CCWGG 1 cut(s) 1276
BstNSI RCATGY 3 cut(s) 813, 859, 1127
BstSCI CCNGG 1 cut(s) 1274
BstSFI CTRYAG 1 cut(s) 1131
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 5 cut(s) 436, 856, 909, 969, 1034
BstV2I GAAGAC 2 cut(s) 128, 1106
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BsuI GTATCC 1 cut(s) 1078
BsuRI GGCC 2 cut(s) 435, 1141
BtgI CCRYGG 1 cut(s) 430
BtsCI GGATG 4 cut(s) 670, 809, 823, 961
BtsIMutI CAGTG 3 cut(s) 129, 459, 1185
Cac8I GCNNGC 1 cut(s) 978
CaiI CAGNNNCTG 1 cut(s) 1275
CfoI GCGC 1 cut(s) 982
Cfr10I RCCGGY 1 cut(s) 928
Cfr13I GGNCC 1 cut(s) 399
CseI GACGC 3 cut(s) 836, 913, 926
Csp6I GTAC 6 cut(s) 58, 445, 535, 689, 807, 1279
CspAI ACCGGT 1 cut(s) 928
CviQI GTAC 6 cut(s) 58, 445, 535, 689, 807, 1279
DdeI CTNAG 5 cut(s) 132, 612, 717, 1089, 1188
DpnI GATC 2 cut(s) 137, 771
DpnII GATC 2 cut(s) 135, 769
EaeI YGGCCR 1 cut(s) 1139
Eco130I CCWWGG 2 cut(s) 395, 430
Eco47I GGWCC 1 cut(s) 399
Eco47III AGCGCT 1 cut(s) 981
Eco57I CTGAAG 2 cut(s) 38, 870
EcoRI GAATTC 2 cut(s) 382, 739
EcoRII CCWGG 1 cut(s) 1274
EcoT14I CCWWGG 2 cut(s) 395, 430
EcoT22I ATGCAT 1 cut(s) 1129
ErhI CCWWGG 2 cut(s) 395, 430
FaqI GGGAC 1 cut(s) 844
FauNDI CATATG 1 cut(s) 1067
Fnu4HI GCNGC 5 cut(s) 425, 845, 923, 983, 1048
FokI GGATG 4 cut(s) 677, 816, 830, 948
Fsp4HI GCNGC 5 cut(s) 425, 845, 923, 983, 1048
FspBI CTAG 2 cut(s) 360, 396
GlaI GCGC 1 cut(s) 981
GluI GCNGC 5 cut(s) 425, 845, 923, 983, 1048
HaeII RGCGCY 1 cut(s) 983
HaeIII GGCC 2 cut(s) 435, 1141
HapII CCGG 1 cut(s) 929
HgaI GACGC 3 cut(s) 836, 913, 926
HhaI GCGC 1 cut(s) 982
Hin6I GCGC 1 cut(s) 980
HinP1I GCGC 1 cut(s) 980
HincII GTYRAC 2 cut(s) 207, 332
HindII GTYRAC 2 cut(s) 207, 332
HinfI GANTC 6 cut(s) 261, 299, 518, 1108, 1192, 1300
HpaI GTTAAC 1 cut(s) 207
HpaII CCGG 1 cut(s) 929
HphI GGTGA 2 cut(s) 106, 943
Hpy166II GTNNAC 4 cut(s) 207, 332, 691, 724
Hpy188I TCNGA 6 cut(s) 257, 551, 615, 1077, 1245, 1325
Hpy188III TCNNGA 6 cut(s) 38, 269, 296, 509, 965, 1196
Hpy8I GTNNAC 4 cut(s) 207, 332, 691, 724
HpyAV CCTTC 3 cut(s) 446, 655, 794
HpyCH4III ACNGT 5 cut(s) 214, 463, 728, 1061, 1249
HpyCH4IV ACGT 1 cut(s) 1281
HpyF10VI GCNNNNNNNGC 6 cut(s) 424, 841, 958, 982, 1133, 1161
HpyF3I CTNAG 5 cut(s) 132, 612, 717, 1089, 1188
HpySE526I ACGT 1 cut(s) 1281
HspAI GCGC 1 cut(s) 980
KspAI GTTAAC 1 cut(s) 207
Kzo9I GATC 2 cut(s) 135, 769
LmnI GCTCC 3 cut(s) 243, 298, 432
Lsp1109I GCAGC 5 cut(s) 436, 856, 909, 969, 1034
LweI GCATC 8 cut(s) 87, 411, 501, 775, 924, 970, 1136, 1276
MaeI CTAG 2 cut(s) 360, 396
MaeII ACGT 1 cut(s) 1281
MaeIII GTNAC 3 cut(s) 631, 1061, 1265
MalI GATC 2 cut(s) 137, 771
MboI GATC 2 cut(s) 135, 769
MboII GAAGA 6 cut(s) 127, 130, 133, 270, 1021, 1106
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 1 cut(s) 186
MlsI TGGCCA 1 cut(s) 1141
MluCI AATT 7 cut(s) 237, 321, 382, 554, 578, 739, 942
MluNI TGGCCA 1 cut(s) 1141
MlyI GAGTC 2 cut(s) 512, 1309
Mox20I TGGCCA 1 cut(s) 1141
Mph1103I ATGCAT 1 cut(s) 1129
MscI TGGCCA 1 cut(s) 1141
MseI TTAA 7 cut(s) 105, 111, 146, 206, 236, 839, 873
MslI CAYNNNNRTG 2 cut(s) 419, 1059
Msp20I TGGCCA 1 cut(s) 1141
MspA1I CMGCKG 1 cut(s) 922
MspCI CTTAAG 1 cut(s) 110
MspI CCGG 1 cut(s) 929
MspR9I CCNGG 1 cut(s) 1276
Mva1269I GAATGC 1 cut(s) 1239
MvaI CCWGG 1 cut(s) 1276
MwoI GCNNNNNNNGC 6 cut(s) 424, 841, 958, 982, 1133, 1161
NcoI CCATGG 1 cut(s) 430
NdeI CATATG 1 cut(s) 1067
NdeII GATC 2 cut(s) 135, 769
NlaIV GGNNCC 4 cut(s) 70, 245, 598, 650
NmeAIII GCCGAG 1 cut(s) 618
NmuCI GTSAC 2 cut(s) 1061, 1265
NsiI ATGCAT 1 cut(s) 1129
NspI RCATGY 3 cut(s) 813, 859, 1127
OliI CACNNNNGTG 1 cut(s) 1059
PciI ACATGT 1 cut(s) 855
PctI GAATGC 1 cut(s) 1239
PfeI GAWTC 4 cut(s) 261, 299, 1108, 1192
PinAI ACCGGT 1 cut(s) 928
PkrI GCNGC 5 cut(s) 426, 846, 924, 984, 1049
PleI GAGTC 2 cut(s) 512, 1308
PpsI GAGTC 2 cut(s) 512, 1308
PscI ACATGT 1 cut(s) 855
PsiI TTATAA 1 cut(s) 1253
Psp6I CCWGG 1 cut(s) 1274
PspGI CCWGG 1 cut(s) 1274
PspN4I GGNNCC 4 cut(s) 70, 245, 598, 650
PspPI GGNCC 1 cut(s) 399
PsrI GAACNNNNNNTAC 2 cut(s) 61, 93
PstNI CAGNNNCTG 1 cut(s) 1275
PsuI RGATCY 1 cut(s) 769
PvuII CAGCTG 1 cut(s) 922
RsaI GTAC 6 cut(s) 59, 446, 536, 690, 808, 1280
RsaNI GTAC 6 cut(s) 58, 445, 535, 689, 807, 1279
RseI CAYNNNNRTG 2 cut(s) 419, 1059
SaqAI TTAA 7 cut(s) 105, 111, 146, 206, 236, 839, 873
SatI GCNGC 5 cut(s) 425, 845, 923, 983, 1048
Sau3AI GATC 2 cut(s) 135, 769
Sau96I GGNCC 1 cut(s) 399
SchI GAGTC 2 cut(s) 512, 1309
ScrFI CCNGG 1 cut(s) 1276
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 8 cut(s) 87, 411, 501, 775, 924, 970, 1136, 1276
SfcI CTRYAG 1 cut(s) 1131
SinI GGWCC 1 cut(s) 399
SmiMI CAYNNNNRTG 2 cut(s) 419, 1059
SmlI CTYRAG 3 cut(s) 110, 591, 731
SmoI CTYRAG 3 cut(s) 110, 591, 731
Sse9I AATT 7 cut(s) 237, 321, 382, 554, 578, 739, 942
SsiI CCGC 1 cut(s) 1237
SspMI CTAG 2 cut(s) 360, 396
StyD4I CCNGG 1 cut(s) 1274
StyI CCWWGG 2 cut(s) 395, 430
TaaI ACNGT 5 cut(s) 214, 463, 728, 1061, 1249
TaiI ACGT 1 cut(s) 1284
TaqI TCGA 2 cut(s) 1007, 1314
TasI AATT 7 cut(s) 237, 321, 382, 554, 578, 739, 942
TatI WGTACW 4 cut(s) 57, 534, 688, 806
TfiI GAWTC 4 cut(s) 261, 299, 1108, 1192
Tru1I TTAA 7 cut(s) 105, 111, 146, 206, 236, 839, 873
Tru9I TTAA 7 cut(s) 105, 111, 146, 206, 236, 839, 873
TscAI CASTG 3 cut(s) 136, 466, 1192
TseFI GTSAC 2 cut(s) 1061, 1265
TseI GCWGC 5 cut(s) 424, 844, 922, 982, 1047
Tsp45I GTSAC 2 cut(s) 1061, 1265
TspDTI ATGAA 3 cut(s) 203, 299, 1002
TspRI CASTG 3 cut(s) 136, 466, 1192
Vha464I CTTAAG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 399
XapI RAATTY 4 cut(s) 382, 554, 578, 739
XceI RCATGY 3 cut(s) 813, 859, 1127
XmaJI CCTAGG 1 cut(s) 395
XspI CTAG 2 cut(s) 360, 396
Zsp2I ATGCAT 1 cut(s) 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.