Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0016993

Physical Location & Seq

Genomic Coordinates Forward (+)

19718832 .. 19721794

Gene Structure 2963 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023192

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1224 bp

ATGGGTCATAAAATTGTTCGTGATCAAGGTGTTAAGAATCCCGGAAAACGCAGTAGGTTGTGGATTGCTAATGATGTTTGTTATGTATTGCAAAATAATATGGGAACTGGAGCAATTGAATGCTTAGTCATGAGCATGGACCACATTTCAGATCTTGACATAAAACCTTCAGCCTTCAAACAGATGCATAATCTAAGATTACTTAAGTTACAGTTTAGGCGGTACTGGGACTCAATGTCATGCATCAACAAAGTTCACCTTCCTCGAGGTCTCGAGTGTCTTCCTCAAGCCCTTAGAATTATGCAGTGGGATGGGTACCCTTTGAATTCTCTGCCATCAACGTTTTGTGCAGAAAAGCTTGTTGAGCTTCGTATGTGGAACAGCCGACTTCAGCGACTTTGGAATGAAGGCCTGAAGATCAATCTTGGGAGCTTAAAACATATCGATCTTAGTGGCTCCAAGCACCTGGCTGATGTTTCATCTCTGATTGAGAGCCCAAATCTCGAGAGAATTAATCTTCAAGGTTGTACAAGTTTGCTTCAACTTCCTTCTTTTCAGCATCTTGCCAAGCTTACTCATCTCAATCTGTACAGCTGCTTCAATCTCAAAATTCTCCCAGAGATGCCAGGCAATCTGCAAACCTTATACTTGGTTCGTACTGCAATAGAAGAATTGCCTTCATCAATTTGGTGTCTCGAGAAGCTTGGTGAATTGGGTCTTGCAGAATGTCAAGAGCTTAAGAATCTTCCAAGCAGCACTTTAATAAAGTGTCTGGAAGGACCAGTGAGTGCTCATCACAGTTGCAACTTCCAAGTAGAACATTCGAGTACATTTCAGTCCATTGCAGACAACGAGACCATAGGTTCAACTGGTGGAACCTTTGAGCTTGGATTCTTCAGTCCCAGTTCTTCTACTACACGATATGTGGGGATATGGTACAAGAAGATATCGGTTACCACCATTTTATGGGTTGCCAACAGAGACACACCCCTCACTGATTCATTAGGTGTTCTCACGATCACACACCGCGGAATTCTTGTCCTTGTCAGCCAAAACAACAGAACAGTTTGGTCCTCCAACACGTCAAGAACTGCACAAAATCCAGTGGCTCAGGTTTCCAACTCCAGAAATCCTGTTGTGAAAGATGGGAGTGATAATGAGGCAGAGAATTTTCGGTGGCGAACTTATGATATTACCCAGGTGACACATTCCTACAAGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000302 GO:0001101 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009627 GO:0009719 GO:0009725 GO:0009733 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009987 GO:0010033 GO:0010035 GO:0010193 GO:0014070 GO:0023052 GO:0032870 GO:0035690 GO:0036094 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0097159 GO:0098542 GO:1901265 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

408

Amino Acids

45.91

Weight (kDa)

8.7

Isoelectric Point (pI)

55.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	320 - 398	2.4e-17	D-mannose binding lectin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016993)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr3g0484811 RchiOBHm_Chr3g0484841 RchiOBHm_Chr3g0484851 RchiOBHm_Chr5g0026491
rosa_laevigata	RLG00000023192
rosa_multiflora	Rmu_co8515397.1_g000001 Rmu_sc0001689.1_g000012 Rmu_sc0001689.1_g000013 Rmu_sc0006653.1_g000008
rosa_rugosa	Rorug03G0213800
rosa_samantha	Rh3AG264300 Rh3AG264400 Rh3CG297100
rosa_wichuraiana	Rw1G000500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	264
Acc65I	GGTACC	1 cut(s)	315
AccB1I	GGYRCC	1 cut(s)	315
AccB7I	CCANNNNNTGG	1 cut(s)	966
AccII	CGCG	1 cut(s)	1029
AciI	CCGC	3 cut(s)	220, 1027, 1029
AclI	AACGTT	1 cut(s)	341
AcsI	RAATTY	4 cut(s)	325, 609, 1032, 1168
AcuI	CTGAAG	4 cut(s)	153, 374, 434, 880
AfaI	GTAC	7 cut(s)	224, 317, 529, 590, 658, 829, 938
AfiI	CCNNNNNNNGG	1 cut(s)	966
AflII	CTTAAG	2 cut(s)	203, 737
AflIII	ACRYGT	1 cut(s)	1080
AgsI	TTSAA	7 cut(s)	119, 178, 325, 521, 542, 601, 867
AhdI	GACNNNNNGTC	1 cut(s)	235
AjiI	CACGTC	1 cut(s)	1083
AjnI	CCWGG	3 cut(s)	465, 625, 1197
AluBI	AGCT	8 cut(s)	358, 367, 432, 571, 594, 703, 736, 886
AluI	AGCT	8 cut(s)	358, 367, 432, 571, 594, 703, 736, 886
Alw21I	GWGCWC	1 cut(s)	793
Alw26I	GTCTC	4 cut(s)	275, 698, 848, 975
Ama87I	CYCGRG	4 cut(s)	264, 272, 503, 695
AoxI	GGCC	1 cut(s)	409
ApeKI	GCWGC	2 cut(s)	594, 753
ApoI	RAATTY	4 cut(s)	325, 609, 1032, 1168
AseI	ATTAAT	1 cut(s)	513
Asp718I	GGTACC	1 cut(s)	315
AspS9I	GGNCC	3 cut(s)	139, 779, 1071
AsuC2I	CCSGG	1 cut(s)	42
AsuHPI	GGTGA	3 cut(s)	248, 719, 1213
AvaI	CYCGRG	4 cut(s)	264, 272, 503, 695
AvaII	GGWCC	3 cut(s)	139, 779, 1071
BanI	GGYRCC	1 cut(s)	315
BanII	GRGCYC	1 cut(s)	497
BarI	GAAGNNNNNNTAC	2 cut(s)	581, 613
BbsI	GAAGAC	1 cut(s)	272
Bbv12I	GWGCWC	1 cut(s)	793
BbvI	GCAGC	2 cut(s)	581, 765
BccI	CCATC	3 cut(s)	305, 343, 1139
BciT130I	CCWGG	3 cut(s)	467, 627, 1199
BclI	TGATCA	1 cut(s)	22
BcnI	CCSGG	1 cut(s)	42
BcoDI	GTCTC	4 cut(s)	275, 698, 848, 975
BfrI	CTTAAG	2 cut(s)	203, 737
BglII	AGATCT	1 cut(s)	151
BisI	GCNGC	2 cut(s)	595, 754
BlsI	GCNGC	2 cut(s)	596, 755
Bme1390I	CCNGG	4 cut(s)	42, 467, 627, 1199
Bme18I	GGWCC	3 cut(s)	139, 779, 1071
BmeRI	GACNNNNNGTC	1 cut(s)	235
BmeT110I	CYCGRG	4 cut(s)	264, 272, 503, 695
BmgBI	CACGTC	1 cut(s)	1083
BmgT120I	GGNCC	3 cut(s)	139, 779, 1071
BmiI	GGNNCC	3 cut(s)	317, 457, 877
BmrFI	CCNGG	4 cut(s)	42, 467, 627, 1199
BmrI	ACTGGG	2 cut(s)	235, 897
BmsI	GCATC	4 cut(s)	174, 252, 568, 612
BmuI	ACTGGG	2 cut(s)	235, 897
BpiI	GAAGAC	1 cut(s)	272
BpmI	CTGGAG	2 cut(s)	129, 1108
Bpu10I	CCTNAGC	1 cut(s)	1110
BpuEI	CTTGAG	1 cut(s)	270
BpuMI	CCSGG	1 cut(s)	42
Bsa29I	ATCGAT	1 cut(s)	444
BsaI	GGTCTC	2 cut(s)	275, 848
BsaJI	CCNNGG	2 cut(s)	1027, 1197
Bsc4I	CCNNNNNNNGG	1 cut(s)	966
Bse1I	ACTGG	6 cut(s)	112, 230, 782, 874, 903, 1103
Bse3DI	GCAATG	1 cut(s)	840
BseBI	CCWGG	3 cut(s)	467, 627, 1199
BseCI	ATCGAT	1 cut(s)	444
BseDI	CCNNGG	2 cut(s)	1027, 1197
BseGI	GGATG	1 cut(s)	316
BseLI	CCNNNNNNNGG	1 cut(s)	966
BseMI	GCAATG	1 cut(s)	840
BseMII	CTCAG	1 cut(s)	1124
BseNI	ACTGG	6 cut(s)	112, 230, 782, 874, 903, 1103
BseXI	GCAGC	2 cut(s)	581, 765
BsgI	GTGCAG	2 cut(s)	369, 1077
Bsh1236I	CGCG	1 cut(s)	1029
BshFI	GGCC	1 cut(s)	411
BshNI	GGYRCC	1 cut(s)	315
BshVI	ATCGAT	1 cut(s)	444
BsiHKAI	GWGCWC	1 cut(s)	793
BsiHKCI	CYCGRG	4 cut(s)	264, 272, 503, 695
BsiSI	CCGG	1 cut(s)	42
BslFI	GGGAC	2 cut(s)	242, 885
BslI	CCNNNNNNNGG	1 cut(s)	966
BsmAI	GTCTC	4 cut(s)	275, 698, 848, 975
BsmFI	GGGAC	2 cut(s)	242, 885
BsmI	GAATGC	1 cut(s)	125
BsnI	GGCC	1 cut(s)	411
Bso31I	GGTCTC	2 cut(s)	275, 848
BsoBI	CYCGRG	4 cut(s)	264, 272, 503, 695
Bsp1286I	GDGCHC	2 cut(s)	497, 793
Bsp1407I	TGTACA	2 cut(s)	527, 588
Bsp143I	GATC	5 cut(s)	22, 151, 417, 445, 1017
BspACI	CCGC	3 cut(s)	220, 1027, 1029
BspANI	GGCC	1 cut(s)	411
BspCNI	CTCAG	1 cut(s)	1123
BspDI	ATCGAT	1 cut(s)	444
BspFNI	CGCG	1 cut(s)	1029
BspHI	TCATGA	1 cut(s)	129
BspLI	GGNNCC	3 cut(s)	317, 457, 877
BspT107I	GGYRCC	1 cut(s)	315
BspTI	CTTAAG	2 cut(s)	203, 737
BspTNI	GGTCTC	2 cut(s)	275, 848
BsrDI	GCAATG	1 cut(s)	840
BsrGI	TGTACA	2 cut(s)	527, 588
BsrI	ACTGG	6 cut(s)	112, 230, 782, 874, 903, 1103
BssECI	CCNNGG	2 cut(s)	1027, 1197
BssMI	GATC	5 cut(s)	22, 151, 417, 445, 1017
Bst2UI	CCWGG	3 cut(s)	467, 627, 1199
Bst4CI	ACNGT	3 cut(s)	213, 800, 1066
BstAFI	CTTAAG	2 cut(s)	203, 737
BstAUI	TGTACA	2 cut(s)	527, 588
BstDEI	CTNAG	5 cut(s)	124, 194, 293, 449, 1110
BstDSI	CCRYGG	1 cut(s)	1027
BstEII	GGTNACC	1 cut(s)	952
BstF5I	GGATG	1 cut(s)	316
BstFNI	CGCG	1 cut(s)	1029
BstKTI	GATC	5 cut(s)	25, 154, 420, 448, 1020
BstMAI	GTCTC	4 cut(s)	275, 698, 848, 975
BstMBI	GATC	5 cut(s)	22, 151, 417, 445, 1017
BstMWI	GCNNNNNNNGC	1 cut(s)	364
BstNI	CCWGG	3 cut(s)	467, 627, 1199
BstPI	GGTNACC	1 cut(s)	952
BstSCI	CCNGG	4 cut(s)	40, 465, 625, 1197
BstUI	CGCG	1 cut(s)	1029
BstV1I	GCAGC	2 cut(s)	581, 765
BstV2I	GAAGAC	1 cut(s)	272
BstX2I	RGATCY	1 cut(s)	151
BstXI	CCANNNNNNTGG	1 cut(s)	466
BstYI	RGATCY	1 cut(s)	151
Bsu15I	ATCGAT	1 cut(s)	444
BsuRI	GGCC	1 cut(s)	411
BsuTUI	ATCGAT	1 cut(s)	444
BtgI	CCRYGG	1 cut(s)	1027
BtrI	CACGTC	1 cut(s)	1083
BtsCI	GGATG	1 cut(s)	316
BtsI	GCAGTG	1 cut(s)	311
BtsIMutI	CAGTG	4 cut(s)	311, 789, 993, 1110
CciI	TCATGA	1 cut(s)	129
Cfr13I	GGNCC	3 cut(s)	139, 779, 1071
Cfr42I	CCGCGG	1 cut(s)	1030
ClaI	ATCGAT	1 cut(s)	444
Csp6I	GTAC	7 cut(s)	223, 316, 528, 589, 657, 828, 937
CviAII	CATG	3 cut(s)	130, 136, 240
CviQI	GTAC	7 cut(s)	223, 316, 528, 589, 657, 828, 937
DdeI	CTNAG	5 cut(s)	124, 194, 293, 449, 1110
DpnI	GATC	5 cut(s)	24, 153, 419, 447, 1019
DpnII	GATC	5 cut(s)	22, 151, 417, 445, 1017
DriI	GACNNNNNGTC	1 cut(s)	235
Eam1105I	GACNNNNNGTC	1 cut(s)	235
Eco147I	AGGCCT	1 cut(s)	411
Eco24I	GRGCYC	1 cut(s)	497
Eco31I	GGTCTC	2 cut(s)	275, 848
Eco32I	GATATC	1 cut(s)	948
Eco47I	GGWCC	3 cut(s)	139, 779, 1071
Eco57I	CTGAAG	4 cut(s)	153, 374, 434, 880
Eco88I	CYCGRG	4 cut(s)	264, 272, 503, 695
Eco91I	GGTNACC	1 cut(s)	952
EcoO65I	GGTNACC	1 cut(s)	952
EcoRI	GAATTC	2 cut(s)	325, 1032
EcoRII	CCWGG	3 cut(s)	465, 625, 1197
EcoRV	GATATC	1 cut(s)	948
EcoT22I	ATGCAT	2 cut(s)	189, 245
EcoT38I	GRGCYC	1 cut(s)	497
FaeI	CATG	3 cut(s)	133, 139, 243
FalI	AAGNNNNNCTT	4 cut(s)	243, 275, 742, 774
FaqI	GGGAC	2 cut(s)	242, 885
FatI	CATG	3 cut(s)	129, 135, 239
FbaI	TGATCA	1 cut(s)	22
Fnu4HI	GCNGC	2 cut(s)	595, 754
FokI	GGATG	1 cut(s)	323
FriOI	GRGCYC	1 cut(s)	497
Fsp4HI	GCNGC	2 cut(s)	595, 754
GluI	GCNGC	2 cut(s)	595, 754
GsuI	CTGGAG	2 cut(s)	129, 1108
HaeIII	GGCC	1 cut(s)	411
HapII	CCGG	1 cut(s)	42
Hin1II	CATG	3 cut(s)	133, 139, 243
HindIII	AAGCTT	3 cut(s)	356, 569, 701
HinfI	GANTC	5 cut(s)	37, 230, 742, 891, 998
HpaII	CCGG	1 cut(s)	42
HphI	GGTGA	3 cut(s)	248, 719, 1213
Hpy166II	GTNNAC	1 cut(s)	256
Hpy188I	TCNGA	2 cut(s)	151, 486
Hpy8I	GTNNAC	1 cut(s)	256
HpyAV	CCTTC	7 cut(s)	177, 184, 269, 401, 558, 687, 770
HpyCH4III	ACNGT	3 cut(s)	213, 800, 1066
HpyCH4IV	ACGT	2 cut(s)	341, 1082
HpyF10VI	GCNNNNNNNGC	1 cut(s)	364
HpyF3I	CTNAG	5 cut(s)	124, 194, 293, 449, 1110
HpySE526I	ACGT	2 cut(s)	341, 1082
Hsp92II	CATG	3 cut(s)	133, 139, 243
KpnI	GGTACC	1 cut(s)	319
Ksp22I	TGATCA	1 cut(s)	22
KspI	CCGCGG	1 cut(s)	1030
Kzo9I	GATC	5 cut(s)	22, 151, 417, 445, 1017
LmnI	GCTCC	3 cut(s)	110, 429, 461
Lsp1109I	GCAGC	2 cut(s)	581, 765
LweI	GCATC	4 cut(s)	174, 252, 568, 612
MaeII	ACGT	2 cut(s)	341, 1082
MaeIII	GTNAC	3 cut(s)	207, 952, 1201
MalI	GATC	5 cut(s)	24, 153, 419, 447, 1019
MboI	GATC	5 cut(s)	22, 151, 417, 445, 1017
MboII	GAAGA	8 cut(s)	272, 427, 509, 680, 737, 886, 900, 955
MfeI	CAATTG	1 cut(s)	114
MflI	RGATCY	1 cut(s)	151
MhlI	GDGCHC	2 cut(s)	497, 793
MlyI	GAGTC	1 cut(s)	224
MmeI	TCCRAC	2 cut(s)	1101, 1143
MnlI	CCTC	6 cut(s)	260, 273, 294, 1001, 1084, 1153
Mph1103I	ATGCAT	2 cut(s)	189, 245
MseI	TTAA	6 cut(s)	33, 204, 434, 513, 738, 761
MslI	CAYNNNNRTG	1 cut(s)	134
MspA1I	CMGCKG	2 cut(s)	594, 1029
MspCI	CTTAAG	2 cut(s)	203, 737
MspI	CCGG	1 cut(s)	42
MspR9I	CCNGG	4 cut(s)	42, 467, 627, 1199
MunI	CAATTG	1 cut(s)	114
Mva1269I	GAATGC	1 cut(s)	125
MvaI	CCWGG	3 cut(s)	467, 627, 1199
MvnI	CGCG	1 cut(s)	1029
MwoI	GCNNNNNNNGC	1 cut(s)	364
NciI	CCSGG	1 cut(s)	42
NdeII	GATC	5 cut(s)	22, 151, 417, 445, 1017
NlaIII	CATG	3 cut(s)	133, 139, 243
NlaIV	GGNNCC	3 cut(s)	317, 457, 877
NmuCI	GTSAC	1 cut(s)	1201
NsiI	ATGCAT	2 cut(s)	189, 245
PaeR7I	CTCGAG	4 cut(s)	264, 272, 503, 695
PagI	TCATGA	1 cut(s)	129
PceI	AGGCCT	1 cut(s)	411
PctI	GAATGC	1 cut(s)	125
PfeI	GAWTC	4 cut(s)	37, 742, 891, 998
PflMI	CCANNNNNTGG	1 cut(s)	966
PfoI	TCCNGGA	1 cut(s)	40
PkrI	GCNGC	2 cut(s)	596, 755
PleI	GAGTC	1 cut(s)	224
PpsI	GAGTC	1 cut(s)	224
PshBI	ATTAAT	1 cut(s)	513
Psp1406I	AACGTT	1 cut(s)	341
Psp6I	CCWGG	3 cut(s)	465, 625, 1197
PspEI	GGTNACC	1 cut(s)	952
PspGI	CCWGG	3 cut(s)	465, 625, 1197
PspN4I	GGNNCC	3 cut(s)	317, 457, 877
PspPI	GGNCC	3 cut(s)	139, 779, 1071
PspXI	VCTCGAGB	1 cut(s)	264
PsuI	RGATCY	1 cut(s)	151
PvuII	CAGCTG	1 cut(s)	594
RsaI	GTAC	7 cut(s)	224, 317, 529, 590, 658, 829, 938
RsaNI	GTAC	7 cut(s)	223, 316, 528, 589, 657, 828, 937
RseI	CAYNNNNRTG	1 cut(s)	134
SacII	CCGCGG	1 cut(s)	1030
SaqAI	TTAA	6 cut(s)	33, 204, 434, 513, 738, 761
SatI	GCNGC	2 cut(s)	595, 754
Sau3AI	GATC	5 cut(s)	22, 151, 417, 445, 1017
Sau96I	GGNCC	3 cut(s)	139, 779, 1071
SchI	GAGTC	1 cut(s)	224
ScrFI	CCNGG	4 cut(s)	42, 467, 627, 1199
SduI	GDGCHC	2 cut(s)	497, 793
SfaNI	GCATC	4 cut(s)	174, 252, 568, 612
Sfr274I	CTCGAG	4 cut(s)	264, 272, 503, 695
Sfr303I	CCGCGG	1 cut(s)	1030
SgrBI	CCGCGG	1 cut(s)	1030
SinI	GGWCC	3 cut(s)	139, 779, 1071
SlaI	CTCGAG	4 cut(s)	264, 272, 503, 695
SmiMI	CAYNNNNRTG	1 cut(s)	134
SmlI	CTYRAG	7 cut(s)	203, 264, 272, 285, 503, 695, 737
SmoI	CTYRAG	7 cut(s)	203, 264, 272, 285, 503, 695, 737
SseBI	AGGCCT	1 cut(s)	411
SsiI	CCGC	3 cut(s)	220, 1027, 1029
StuI	AGGCCT	1 cut(s)	411
StyD4I	CCNGG	4 cut(s)	40, 465, 625, 1197
TaaI	ACNGT	3 cut(s)	213, 800, 1066
TaiI	ACGT	2 cut(s)	344, 1085
TaqI	TCGA	6 cut(s)	265, 273, 444, 504, 696, 824
TatI	WGTACW	3 cut(s)	527, 588, 827
TfiI	GAWTC	4 cut(s)	37, 742, 891, 998
Tru1I	TTAA	6 cut(s)	33, 204, 434, 513, 738, 761
Tru9I	TTAA	6 cut(s)	33, 204, 434, 513, 738, 761
TscAI	CASTG	4 cut(s)	311, 789, 1000, 1110
TseFI	GTSAC	1 cut(s)	1201
TseI	GCWGC	2 cut(s)	594, 753
Tsp45I	GTSAC	1 cut(s)	1201
TspDTI	ATGAA	4 cut(s)	420, 468, 669, 990
TspRI	CASTG	4 cut(s)	311, 789, 1000, 1110
Van91I	CCANNNNNTGG	1 cut(s)	966
Vha464I	CTTAAG	2 cut(s)	203, 737
VpaK11BI	GGWCC	3 cut(s)	139, 779, 1071
VspI	ATTAAT	1 cut(s)	513
XapI	RAATTY	4 cut(s)	325, 609, 1032, 1168
XhoI	CTCGAG	4 cut(s)	264, 272, 503, 695
Zsp2I	ATGCAT	2 cut(s)	189, 245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.