RLG00000024457

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018175
Physical Location & Seq
Reverse (-)
35997843 .. 35999354
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024457

Sequence Viewer

Length: 1218 bp
ATGCCTCCTCCGGGTCGAGCAGCCGTCGCTACAATCCTCGCATTCTGTCTCCTCCTCCTCAACAATGTCTCCGCCACCGAGCACACCTACATTGCCTCGCAGCAGGTGACGGCGCAGGACTTCCTCCAGGCCCACAACGAGGTCAGGGCCATGTTCAACGAACCGATGCTAACTTGGGACGATAAGCTGGCCGACTACGCCCACCAGTGGGCCGAGAAGACCAGCTCCCTTGGGAAATGCGAGGAGCGTTACCACTCCAACGGCCCCTACGGCGAGAACCTCTTTTGGGGGTGGTCGTGGAACGACTACTTTAGCCCTAGAGACGCCGTCATGATGTGGACCGAGGAGAAAAACGACTTCGACGTCAAGACGTTGATGTGCACGCCGGGGAAAATGTGCGGCCACTTCACGCAGGTCGTGTGGAGGAACTCCAAGAGAGTAGGGTGCAGCCGCATCAAGTGCGCCCAAGGCGGAGTGTTGATGATTTGTAATTATGATCCTCCTGGGAATTGGATTGATTCGAAACTTGGAGGTCTCATGAATCCCTTCGGCGAGGATAAGACGACAGTGTCGCCTGTTTTGGCTGAACAAGTGTCGCAAGCAGTACCGCATGTTGTTCAGCAGAAAGTGCCGCAACCGGTGCCCGTGGATGGTCATCCGACAGTGCCGCACCCGGTGCCGGCTGACGGTCATCCGACAGTGCCGCACCCTGTGCCAGCTGATGGTCATCAGAAAGTGCCGCAACCGGTGCCGGCTGACGGTCATCCGACAGTGCCGCACCACCCAATGCCGACTGACGGTCATCCGACAGTGCTGCACCCGGTGCGGGCTGACGGTCATCCGACAGTGCCGCACCACCCGGTGCCGGCTGACGGTCGTCCGACAGTGCCGCACCCTGTGCCGGCTGATGGTCATCTGATAGTGCCGCACCCGGTGCCGGCTGAAGGTCATCCGACAGTGCCGCACCCGGTGCAGGCTGAACATCAGCCGGCATTCTCCCGACCGCCGCAGTCTGGCGGAGAGTTGCCAAAGAAAGGAAGAGGAAGAAACAGAAGGGGTCAAGGGAGAGGAGGTCATCATCCTGGTCGTCCTAAACCTAACCAGAACCGTAGTCATCGTAACCGTAACCGTAACCGTAACCATCACCACCTTAGCCCCCGTCAGCGCTATCTTAATCGCTTCGGTCAACGTCGTCAACTTGATAATCGTATACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

406

Amino Acids

45.02

Weight (kDa)

9.17

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 42 - 165 1e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018175)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0468031
rosa_laevigata RLG00000024457
rosa_multiflora Rmu_co8380321.1_g000001
rosa_roxburghii Rroxscaffold_6G00413160
rosa_rugosa Rorug03G0091600 Rorug03G0091700 Rorug03G0091800
rosa_samantha Rh3BG161600 Rh3CG161600 Rh3DG162100
rosa_wichuraiana Rw3G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
AasI GACNNNNNNGTC 2 cut(s) 362, 568
AatII GACGTC 1 cut(s) 366
Acc36I ACCTGC 2 cut(s) 94, 403
AccB1I GGYRCC 5 cut(s) 640, 676, 748, 862, 934
AccB7I CCANNNNNTGG 1 cut(s) 722
AccI GTMKAC 1 cut(s) 1210
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 2 cut(s) 189, 400
AcuI CTGAAG 1 cut(s) 963
AcyI GRCGYC 2 cut(s) 324, 363
AdeI CACNNNGTG 7 cut(s) 676, 712, 823, 862, 898, 934, 970
AfaI GTAC 1 cut(s) 606
AfeI AGCGCT 1 cut(s) 1166
AgeI ACCGGT 2 cut(s) 637, 745
AgsI TTSAA 1 cut(s) 157
AhdI GACNNNNNGTC 1 cut(s) 798
AjnI CCWGG 3 cut(s) 126, 502, 1081
AluBI AGCT 3 cut(s) 187, 225, 719
AluI AGCT 3 cut(s) 187, 225, 719
Alw21I GWGCWC 2 cut(s) 84, 383
Alw26I GTCTC 4 cut(s) 53, 73, 315, 539
Alw44I GTGCAC 1 cut(s) 379
AlwI GGATC 1 cut(s) 491
Aor51HI AGCGCT 1 cut(s) 1166
AoxI GGCC 6 cut(s) 129, 147, 189, 210, 262, 400
ApaLI GTGCAC 1 cut(s) 379
ApeKI GCWGC 4 cut(s) 20, 100, 447, 814
AsiGI ACCGGT 2 cut(s) 637, 745
Asp700I GAANNNNTTC 1 cut(s) 545
AspLEI GCGC 3 cut(s) 115, 464, 1167
AspS9I GGNCC 5 cut(s) 130, 147, 210, 263, 339
AsuC2I CCSGG 7 cut(s) 12, 387, 674, 821, 860, 932, 968
AsuHPI GGTGA 2 cut(s) 118, 1136
AsuII TTCGAA 1 cut(s) 521
AvaII GGWCC 1 cut(s) 339
BaeGI GKGCMC 2 cut(s) 383, 645
BanI GGYRCC 5 cut(s) 640, 676, 748, 862, 934
BbsI GAAGAC 1 cut(s) 224
Bbv12I GWGCWC 2 cut(s) 84, 383
BbvI GCAGC 4 cut(s) 32, 112, 459, 801
BccI CCATC 4 cut(s) 644, 716, 902, 1149
BceAI ACGGC 5 cut(s) 8, 126, 277, 286, 311
BcgI CGANNNNNNTGC 2 cut(s) 796, 830
BciT130I CCWGG 3 cut(s) 128, 504, 1083
BcnI CCSGG 7 cut(s) 12, 387, 674, 821, 860, 932, 968
BcoDI GTCTC 4 cut(s) 53, 73, 315, 539
BfaI CTAG 1 cut(s) 318
BfoI RGCGCY 1 cut(s) 1168
BfuAI ACCTGC 2 cut(s) 94, 403
BglI GCCNNNNNGGC 1 cut(s) 270
Bme18I GGWCC 1 cut(s) 339
BmeRI GACNNNNNGTC 1 cut(s) 798
BmgT120I GGNCC 5 cut(s) 130, 147, 210, 263, 339
BmiI GGNNCC 6 cut(s) 265, 642, 678, 750, 864, 936
BmsI GCATC 2 cut(s) 156, 462
BoxI GACNNNNGTC 1 cut(s) 876
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 1151
Bpu14I TTCGAA 1 cut(s) 521
BpuMI CCSGG 7 cut(s) 12, 387, 674, 821, 860, 932, 968
BsaBI GATNNNNATC 3 cut(s) 654, 726, 912
BsaHI GRCGYC 2 cut(s) 324, 363
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 6 cut(s) 229, 342, 386, 466, 503, 645
BsaWI WCCGGW 2 cut(s) 637, 745
BsaXI ACNNNNNCTCC 4 cut(s) 53, 83, 1057, 1087
Bse118I RCCGGY 8 cut(s) 637, 679, 745, 751, 865, 901, 937, 988
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 90
Bse8I GATNNNNATC 3 cut(s) 654, 726, 912
BseBI CCWGG 3 cut(s) 128, 504, 1083
BseDI CCNNGG 6 cut(s) 229, 342, 386, 466, 503, 645
BseGI GGATG 8 cut(s) 655, 655, 691, 763, 802, 838, 949, 1078
BseJI GATNNNNATC 3 cut(s) 654, 726, 912
BseMI GCAATG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 6 cut(s) 41, 44, 47, 257, 359, 1083
BseSI GKGCMC 2 cut(s) 383, 645
BseXI GCAGC 4 cut(s) 32, 112, 459, 801
BsgI GTGCAG 3 cut(s) 466, 800, 992
Bsh1285I CGRYCG 2 cut(s) 877, 1004
BshFI GGCC 6 cut(s) 131, 149, 191, 212, 264, 402
BshNI GGYRCC 5 cut(s) 640, 676, 748, 862, 934
BshTI ACCGGT 2 cut(s) 637, 745
BsiEI CGRYCG 2 cut(s) 877, 1004
BsiHKAI GWGCWC 2 cut(s) 84, 383
BslFI GGGAC 1 cut(s) 191
BsmAI GTCTC 4 cut(s) 53, 73, 315, 539
BsmBI CGTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 2 cut(s) 41, 992
BsnI GGCC 6 cut(s) 131, 149, 191, 212, 264, 402
Bso31I GGTCTC 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 3 cut(s) 84, 383, 645
Bsp143I GATC 1 cut(s) 496
BspANI GGCC 6 cut(s) 131, 149, 191, 212, 264, 402
BspHI TCATGA 2 cut(s) 330, 537
BspLI GGNNCC 6 cut(s) 265, 642, 678, 750, 864, 936
BspMI ACCTGC 2 cut(s) 94, 403
BspPI GGATC 1 cut(s) 491
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 5 cut(s) 640, 676, 748, 862, 934
BspTNI GGTCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 8 cut(s) 637, 679, 745, 751, 865, 901, 937, 988
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 8 cut(s) 637, 679, 745, 751, 865, 901, 937, 988
BssECI CCNNGG 6 cut(s) 229, 342, 386, 466, 503, 645
BssMI GATC 1 cut(s) 496
BssNAI GTATAC 1 cut(s) 1211
BssNI GRCGYC 2 cut(s) 324, 363
BssT1I CCWWGG 2 cut(s) 229, 466
Bst1107I GTATAC 1 cut(s) 1211
Bst2UI CCWGG 3 cut(s) 128, 504, 1083
Bst6I CTCTTC 1 cut(s) 1033
BstACI GRCGYC 2 cut(s) 324, 363
BstBI TTCGAA 1 cut(s) 521
BstDEI CTNAG 1 cut(s) 1151
BstDSI CCRYGG 1 cut(s) 645
BstF5I GGATG 8 cut(s) 655, 655, 691, 763, 802, 838, 949, 1078
BstH2I RGCGCY 1 cut(s) 1168
BstHHI GCGC 3 cut(s) 115, 464, 1167
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 4 cut(s) 53, 73, 315, 539
BstMBI GATC 1 cut(s) 496
BstMCI CGRYCG 2 cut(s) 877, 1004
BstNI CCWGG 3 cut(s) 128, 504, 1083
BstNSI RCATGY 1 cut(s) 614
BstPAI GACNNNNGTC 1 cut(s) 876
BstSLI GKGCMC 2 cut(s) 383, 645
BstV1I GCAGC 4 cut(s) 32, 112, 459, 801
BstV2I GAAGAC 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 1211
BsuRI GGCC 6 cut(s) 131, 149, 191, 212, 264, 402
BtgI CCRYGG 1 cut(s) 645
BtsCI GGATG 8 cut(s) 655, 655, 691, 763, 802, 838, 949, 1078
BtsIMutI CAGTG 9 cut(s) 212, 573, 669, 705, 777, 816, 852, 891, 963
BveI ACCTGC 2 cut(s) 94, 403
CciI TCATGA 2 cut(s) 330, 537
CfoI GCGC 3 cut(s) 115, 464, 1167
Cfr10I RCCGGY 8 cut(s) 637, 679, 745, 751, 865, 901, 937, 988
Cfr13I GGNCC 5 cut(s) 130, 147, 210, 263, 339
CseI GACGC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 605
CspAI ACCGGT 2 cut(s) 637, 745
CviAII CATG 4 cut(s) 151, 331, 538, 611
CviQI GTAC 1 cut(s) 605
DdeI CTNAG 1 cut(s) 1151
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
DraIII CACNNNGTG 7 cut(s) 676, 712, 823, 862, 898, 934, 970
DrdI GACNNNNNNGTC 2 cut(s) 362, 568
DriI GACNNNNNGTC 1 cut(s) 798
DseDI GACNNNNNNGTC 2 cut(s) 362, 568
EaeI YGGCCR 2 cut(s) 189, 400
Eam1104I CTCTTC 1 cut(s) 1033
Eam1105I GACNNNNNGTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 1033
EciI GGCGGA 3 cut(s) 61, 486, 1032
Eco130I CCWWGG 2 cut(s) 229, 466
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 339
Eco47III AGCGCT 1 cut(s) 1166
Eco57I CTGAAG 1 cut(s) 963
EcoRII CCWGG 3 cut(s) 126, 502, 1081
EcoT14I CCWWGG 2 cut(s) 229, 466
ErhI CCWWGG 2 cut(s) 229, 466
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 4 cut(s) 154, 334, 541, 614
FaiI YATR 6 cut(s) 152, 332, 495, 539, 612, 1211
FaqI GGGAC 1 cut(s) 191
FatI CATG 4 cut(s) 150, 330, 537, 610
FauI CCCGC 1 cut(s) 819
FblI GTMKAC 1 cut(s) 1210
FokI GGATG 8 cut(s) 642, 662, 678, 750, 789, 825, 936, 1065
FspBI CTAG 1 cut(s) 318
GlaI GCGC 3 cut(s) 114, 463, 1166
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 1168
HaeIII GGCC 6 cut(s) 131, 149, 191, 212, 264, 402
HgaI GACGC 1 cut(s) 332
HhaI GCGC 3 cut(s) 115, 464, 1167
Hin1I GRCGYC 2 cut(s) 324, 363
Hin1II CATG 4 cut(s) 154, 334, 541, 614
Hin6I GCGC 3 cut(s) 113, 462, 1165
HinP1I GCGC 3 cut(s) 113, 462, 1165
HincII GTYRAC 2 cut(s) 1187, 1196
HindII GTYRAC 2 cut(s) 1187, 1196
HinfI GANTC 2 cut(s) 518, 541
HphI GGTGA 2 cut(s) 118, 1136
Hpy166II GTNNAC 5 cut(s) 339, 381, 1187, 1196, 1211
Hpy188I TCNGA 9 cut(s) 660, 696, 732, 768, 807, 843, 882, 918, 954
Hpy188III TCNNGA 4 cut(s) 331, 367, 538, 999
Hpy8I GTNNAC 5 cut(s) 339, 381, 1187, 1196, 1211
Hpy99I CGWCG 3 cut(s) 29, 365, 1194
HpyAV CCTTC 3 cut(s) 556, 938, 1047
HpyCH4IV ACGT 3 cut(s) 363, 371, 1189
HpyCH4V TGCA 4 cut(s) 381, 447, 817, 973
HpyF3I CTNAG 1 cut(s) 1151
HpySE526I ACGT 3 cut(s) 363, 371, 1189
Hsp92I GRCGYC 2 cut(s) 324, 363
Hsp92II CATG 4 cut(s) 154, 334, 541, 614
HspAI GCGC 3 cut(s) 113, 462, 1165
KroI GCCGGC 6 cut(s) 679, 751, 865, 901, 937, 988
KroNI GCCGGC 6 cut(s) 681, 753, 867, 903, 939, 990
Kzo9I GATC 1 cut(s) 496
LmnI GCTCC 2 cut(s) 230, 244
Lsp1109I GCAGC 4 cut(s) 32, 112, 459, 801
LweI GCATC 2 cut(s) 156, 462
MaeI CTAG 1 cut(s) 318
MaeII ACGT 3 cut(s) 363, 371, 1189
MaeIII GTNAC 6 cut(s) 106, 248, 1118, 1124, 1130, 1136
MalI GATC 1 cut(s) 498
MboI GATC 1 cut(s) 496
MboII GAAGA 3 cut(s) 229, 1050, 1056
MhlI GDGCHC 3 cut(s) 84, 383, 645
MluCI AATT 2 cut(s) 490, 508
MmeI TCCRAC 8 cut(s) 282, 683, 719, 791, 830, 866, 905, 977
MroNI GCCGGC 6 cut(s) 679, 751, 865, 901, 937, 988
MroXI GAANNNNTTC 1 cut(s) 545
MseI TTAA 1 cut(s) 1173
MspA1I CMGCKG 1 cut(s) 719
Mva1269I GAATGC 2 cut(s) 41, 992
MvaI CCWGG 3 cut(s) 128, 504, 1083
NaeI GCCGGC 6 cut(s) 681, 753, 867, 903, 939, 990
NciI CCSGG 7 cut(s) 12, 387, 674, 821, 860, 932, 968
NdeII GATC 1 cut(s) 496
NgoMIV GCCGGC 6 cut(s) 679, 751, 865, 901, 937, 988
NlaIII CATG 4 cut(s) 154, 334, 541, 614
NlaIV GGNNCC 6 cut(s) 265, 642, 678, 750, 864, 936
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 106
NspI RCATGY 1 cut(s) 614
NspV TTCGAA 1 cut(s) 521
PagI TCATGA 2 cut(s) 330, 537
PaqCI CACCTGC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 2 cut(s) 41, 992
PdiI GCCGGC 6 cut(s) 681, 753, 867, 903, 939, 990
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 2 cut(s) 518, 541
PflFI GACNNNGTC 2 cut(s) 326, 568
PflMI CCANNNNNTGG 1 cut(s) 722
PinAI ACCGGT 2 cut(s) 637, 745
PshAI GACNNNNGTC 1 cut(s) 876
Psp6I CCWGG 3 cut(s) 126, 502, 1081
PspGI CCWGG 3 cut(s) 126, 502, 1081
PspN4I GGNNCC 6 cut(s) 265, 642, 678, 750, 864, 936
PspPI GGNCC 5 cut(s) 130, 147, 210, 263, 339
PsyI GACNNNGTC 2 cut(s) 326, 568
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 1 cut(s) 606
RsaNI GTAC 1 cut(s) 605
SaqAI TTAA 1 cut(s) 1173
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 5 cut(s) 130, 147, 210, 263, 339
SduI GDGCHC 3 cut(s) 84, 383, 645
SfaNI GCATC 2 cut(s) 156, 462
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 1 cut(s) 339
Sse9I AATT 2 cut(s) 490, 508
SspMI CTAG 1 cut(s) 318
StyI CCWWGG 2 cut(s) 229, 466
TaiI ACGT 3 cut(s) 366, 374, 1192
TaqI TCGA 3 cut(s) 16, 360, 521
TaqII GACCGA 2 cut(s) 356, 1172
TasI AATT 2 cut(s) 490, 508
TfiI GAWTC 2 cut(s) 518, 541
Tru1I TTAA 1 cut(s) 1173
Tru9I TTAA 1 cut(s) 1173
TscAI CASTG 9 cut(s) 212, 573, 669, 705, 777, 816, 852, 891, 963
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 4 cut(s) 20, 100, 447, 814
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 554
TspRI CASTG 9 cut(s) 212, 573, 669, 705, 777, 816, 852, 891, 963
Tth111I GACNNNGTC 2 cut(s) 326, 568
Van91I CCANNNNNTGG 1 cut(s) 722
VneI GTGCAC 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 339
XceI RCATGY 1 cut(s) 614
XmiI GTMKAC 1 cut(s) 1210
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 1 cut(s) 318
ZraI GACGTC 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.