Rmu_co8380321.1_g000001

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8380321.1
OG0018175
Physical Location & Seq
Reverse (-)
2 .. 714
713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8380321.1_g000001.1.cds

Sequence Viewer

Length: 713 bp
atgcttcctccgggtcgagcagccgtcgctgcaatcctcatattctgtctcctcctcctcaacaatgtttccgccaccgagtacacctacattgcctcgcagcaggtgacggcgcaggacttcctccaggcccacaacgaggtcagggccatgttcaacgagccgatgctaacgtgggatgataagctggctgactacgcccaccagtgggctgagaagaccagcgccctagggaaatgcgaggagcggtaccactccaacggcccttatggcgagaacctcttttgggggtggtcgtggaacgactactttagccctagagacgccgtcatgatgtggaccgaggagaaaaacgacttcgacgtcaagtcgttgacgtgcatgccggggaaaatgtgcggccacttcacgcaggtcgtgtggaggaactccaagagagtagggtgcagccgcatcaagtgcgcccaaggcggcgtgttgatgatttgtaattatgatcctcctgggaattggattgattcgaaacttggaggtctcatgaatcccttcagcgaggaaaagacgacagtgccgcctgtttcggctgaacaagtgtcgcaagcagtaccgcatgttgttcagccgaaagtaccgcaaccggtgccggttgatggttatccgacagtgctgcccgtttcggctgaacaagtgtcgcaagcagtaccgcatgttgt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

238

Amino Acids

26.58

Weight (kDa)

5.49

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018175)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0468031
rosa_laevigata RLG00000024457
rosa_multiflora Rmu_co8380321.1_g000001
rosa_roxburghii Rroxscaffold_6G00413160
rosa_rugosa Rorug03G0091600 Rorug03G0091700 Rorug03G0091800
rosa_samantha Rh3BG161600 Rh3CG161600 Rh3DG162100
rosa_wichuraiana Rw3G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
AasI GACNNNNNNGTC 1 cut(s) 362
AatII GACGTC 1 cut(s) 366
Acc36I ACCTGC 2 cut(s) 94, 403
Acc65I GGTACC 1 cut(s) 249
AccB1I GGYRCC 2 cut(s) 249, 640
AccBSI CCGCTC 1 cut(s) 247
AciI CCGC 9 cut(s) 72, 247, 399, 451, 471, 572, 608, 632, 704
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 532
AcyI GRCGYC 2 cut(s) 324, 363
AfaI GTAC 5 cut(s) 83, 251, 606, 630, 702
AfiI CCNNNNNNNGG 5 cut(s) 139, 207, 208, 286, 650
AgeI ACCGGT 1 cut(s) 637
AgsI TTSAA 1 cut(s) 157
AhdI GACNNNNNGTC 1 cut(s) 367
AjiI CACGTC 1 cut(s) 378
AjnI CCWGG 2 cut(s) 126, 502
AluBI AGCT 1 cut(s) 187
AluI AGCT 1 cut(s) 187
Alw26I GTCTC 3 cut(s) 53, 315, 539
AlwI GGATC 1 cut(s) 491
AoxI GGCC 4 cut(s) 129, 147, 262, 400
ApeKI GCWGC 5 cut(s) 20, 29, 100, 447, 667
AsiGI ACCGGT 1 cut(s) 637
Asp700I GAANNNNTTC 1 cut(s) 545
Asp718I GGTACC 1 cut(s) 249
AspA2I CCTAGG 1 cut(s) 229
AspLEI GCGC 3 cut(s) 115, 227, 464
AspS9I GGNCC 4 cut(s) 130, 147, 263, 339
AsuC2I CCSGG 2 cut(s) 12, 387
AsuHPI GGTGA 1 cut(s) 118
AsuII TTCGAA 1 cut(s) 521
AvaII GGWCC 1 cut(s) 339
AvrII CCTAGG 1 cut(s) 229
BanI GGYRCC 2 cut(s) 249, 640
BbsI GAAGAC 1 cut(s) 224
BbvI GCAGC 5 cut(s) 16, 32, 112, 459, 654
BccI CCATC 1 cut(s) 644
BceAI ACGGC 4 cut(s) 8, 126, 277, 311
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 2 cut(s) 128, 504
BcnI CCSGG 2 cut(s) 12, 387
BcoDI GTCTC 3 cut(s) 53, 315, 539
BfaI CTAG 2 cut(s) 230, 318
BfoI RGCGCY 1 cut(s) 228
BfuAI ACCTGC 2 cut(s) 94, 403
BglI GCCNNNNNGGC 1 cut(s) 270
BisI GCNGC 9 cut(s) 21, 30, 101, 400, 448, 451, 472, 572, 668
BlnI CCTAGG 1 cut(s) 229
BlsI GCNGC 9 cut(s) 22, 31, 102, 401, 449, 452, 473, 573, 669
Bme1390I CCNGG 4 cut(s) 12, 128, 387, 504
Bme18I GGWCC 1 cut(s) 339
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 378
BmgT120I GGNCC 4 cut(s) 130, 147, 263, 339
BmiI GGNNCC 2 cut(s) 251, 642
BmrFI CCNGG 4 cut(s) 12, 128, 387, 504
BmsI GCATC 2 cut(s) 156, 462
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 521
BpuMI CCSGG 2 cut(s) 12, 387
BsaBI GATNNNNATC 1 cut(s) 654
BsaHI GRCGYC 2 cut(s) 324, 363
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BsaWI WCCGGW 1 cut(s) 637
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 207, 208, 286, 650
Bse118I RCCGGY 2 cut(s) 637, 643
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 90
Bse8I GATNNNNATC 1 cut(s) 654
BseBI CCWGG 2 cut(s) 128, 504
BseDI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BseGI GGATG 1 cut(s) 184
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 5 cut(s) 139, 207, 208, 286, 650
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 5 cut(s) 41, 44, 47, 257, 359
BseXI GCAGC 5 cut(s) 16, 32, 112, 459, 654
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 4 cut(s) 131, 149, 264, 402
BshNI GGYRCC 2 cut(s) 249, 640
BshTI ACCGGT 1 cut(s) 637
BsiSI CCGG 4 cut(s) 11, 386, 638, 644
BslI CCNNNNNNNGG 5 cut(s) 139, 207, 208, 286, 650
BsmAI GTCTC 3 cut(s) 53, 315, 539
BsmBI CGTCTC 1 cut(s) 315
BsnI GGCC 4 cut(s) 131, 149, 264, 402
Bso31I GGTCTC 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 521
Bsp143I GATC 1 cut(s) 496
BspACI CCGC 9 cut(s) 72, 247, 399, 451, 471, 572, 608, 632, 704
BspANI GGCC 4 cut(s) 131, 149, 264, 402
BspCNI CTCAG 1 cut(s) 205
BspHI TCATGA 2 cut(s) 330, 537
BspLI GGNNCC 2 cut(s) 251, 642
BspMI ACCTGC 2 cut(s) 94, 403
BspPI GGATC 1 cut(s) 491
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 2 cut(s) 249, 640
BspTNI GGTCTC 1 cut(s) 539
BsrBI CCGCTC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 637, 643
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 2 cut(s) 637, 643
BssECI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BssMI GATC 1 cut(s) 496
BssNI GRCGYC 2 cut(s) 324, 363
BssT1I CCWWGG 2 cut(s) 229, 466
Bst2UI CCWGG 2 cut(s) 128, 504
Bst4CI ACNGT 2 cut(s) 568, 664
BstACI GRCGYC 2 cut(s) 324, 363
BstAPI GCANNNNNTGC 2 cut(s) 459, 640
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 189, 383, 600, 696
BstDEI CTNAG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 184
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 3 cut(s) 115, 227, 464
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 3 cut(s) 53, 315, 539
BstMBI GATC 1 cut(s) 496
BstMWI GCNNNNNNNGC 7 cut(s) 26, 29, 197, 270, 459, 468, 640
BstNI CCWGG 2 cut(s) 128, 504
BstNSI RCATGY 3 cut(s) 385, 614, 710
BstSCI CCNGG 4 cut(s) 10, 126, 385, 502
BstV1I GCAGC 5 cut(s) 16, 32, 112, 459, 654
BstV2I GAAGAC 1 cut(s) 224
BsuRI GGCC 4 cut(s) 131, 149, 264, 402
BtrI CACGTC 1 cut(s) 378
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 3 cut(s) 212, 573, 669
BveI ACCTGC 2 cut(s) 94, 403
Cac8I GCNNGC 4 cut(s) 189, 383, 600, 696
CciI TCATGA 2 cut(s) 330, 537
CfoI GCGC 3 cut(s) 115, 227, 464
Cfr10I RCCGGY 2 cut(s) 637, 643
Cfr13I GGNCC 4 cut(s) 130, 147, 263, 339
CseI GACGC 1 cut(s) 332
Csp6I GTAC 5 cut(s) 82, 250, 605, 629, 701
CspAI ACCGGT 1 cut(s) 637
CviAII CATG 6 cut(s) 151, 331, 382, 538, 611, 707
CviQI GTAC 5 cut(s) 82, 250, 605, 629, 701
DdeI CTNAG 1 cut(s) 213
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
DrdI GACNNNNNNGTC 1 cut(s) 362
DriI GACNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 400
Eam1105I GACNNNNNGTC 1 cut(s) 367
EciI GGCGGA 1 cut(s) 61
Eco130I CCWWGG 2 cut(s) 229, 466
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 339
Eco57I CTGAAG 1 cut(s) 532
EcoRII CCWGG 2 cut(s) 126, 502
EcoT14I CCWWGG 2 cut(s) 229, 466
ErhI CCWWGG 2 cut(s) 229, 466
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 6 cut(s) 154, 334, 385, 541, 614, 710
FaiI YATR 9 cut(s) 41, 152, 270, 332, 383, 495, 539, 612, 708
FatI CATG 6 cut(s) 150, 330, 381, 537, 610, 706
Fnu4HI GCNGC 9 cut(s) 21, 30, 101, 400, 448, 451, 472, 572, 668
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 9 cut(s) 21, 30, 101, 400, 448, 451, 472, 572, 668
FspBI CTAG 2 cut(s) 230, 318
GlaI GCGC 3 cut(s) 114, 226, 463
GluI GCNGC 9 cut(s) 21, 30, 101, 400, 448, 451, 472, 572, 668
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 228
HaeIII GGCC 4 cut(s) 131, 149, 264, 402
HapII CCGG 4 cut(s) 11, 386, 638, 644
HgaI GACGC 1 cut(s) 332
HhaI GCGC 3 cut(s) 115, 227, 464
Hin1I GRCGYC 2 cut(s) 324, 363
Hin1II CATG 6 cut(s) 154, 334, 385, 541, 614, 710
Hin6I GCGC 3 cut(s) 113, 225, 462
HinP1I GCGC 3 cut(s) 113, 225, 462
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 2 cut(s) 518, 541
HpaII CCGG 4 cut(s) 11, 386, 638, 644
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 3 cut(s) 84, 339, 375
Hpy188I TCNGA 1 cut(s) 660
Hpy188III TCNNGA 2 cut(s) 331, 538
Hpy8I GTNNAC 3 cut(s) 84, 339, 375
Hpy99I CGWCG 2 cut(s) 29, 365
HpyAV CCTTC 1 cut(s) 556
HpyCH4III ACNGT 2 cut(s) 568, 664
HpyCH4IV ACGT 3 cut(s) 173, 363, 377
HpyCH4V TGCA 3 cut(s) 32, 381, 447
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 29, 197, 270, 459, 468, 640
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 3 cut(s) 173, 363, 377
Hsp92I GRCGYC 2 cut(s) 324, 363
Hsp92II CATG 6 cut(s) 154, 334, 385, 541, 614, 710
HspAI GCGC 3 cut(s) 113, 225, 462
KpnI GGTACC 1 cut(s) 253
Kzo9I GATC 1 cut(s) 496
LmnI GCTCC 1 cut(s) 244
Lsp1109I GCAGC 5 cut(s) 16, 32, 112, 459, 654
LweI GCATC 2 cut(s) 156, 462
MaeI CTAG 2 cut(s) 230, 318
MaeII ACGT 3 cut(s) 173, 363, 377
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 498
MbiI CCGCTC 1 cut(s) 247
MboI GATC 1 cut(s) 496
MboII GAAGA 1 cut(s) 229
MluCI AATT 2 cut(s) 490, 508
MmeI TCCRAC 2 cut(s) 282, 683
MroXI GAANNNNTTC 1 cut(s) 545
MspI CCGG 4 cut(s) 11, 386, 638, 644
MspR9I CCNGG 4 cut(s) 12, 128, 387, 504
MvaI CCWGG 2 cut(s) 128, 504
MwoI GCNNNNNNNGC 7 cut(s) 26, 29, 197, 270, 459, 468, 640
NciI CCSGG 2 cut(s) 12, 387
NdeII GATC 1 cut(s) 496
NlaIII CATG 6 cut(s) 154, 334, 385, 541, 614, 710
NlaIV GGNNCC 2 cut(s) 251, 642
NmuCI GTSAC 1 cut(s) 106
NspI RCATGY 3 cut(s) 385, 614, 710
NspV TTCGAA 1 cut(s) 521
PaeI GCATGC 1 cut(s) 385
PagI TCATGA 2 cut(s) 330, 537
PaqCI CACCTGC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 2 cut(s) 518, 541
PflFI GACNNNGTC 1 cut(s) 326
PinAI ACCGGT 1 cut(s) 637
PkrI GCNGC 9 cut(s) 22, 31, 102, 401, 449, 452, 473, 573, 669
Psp6I CCWGG 2 cut(s) 126, 502
PspGI CCWGG 2 cut(s) 126, 502
PspN4I GGNNCC 2 cut(s) 251, 642
PspPI GGNCC 4 cut(s) 130, 147, 263, 339
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 5 cut(s) 83, 251, 606, 630, 702
RsaNI GTAC 5 cut(s) 82, 250, 605, 629, 701
SatI GCNGC 9 cut(s) 21, 30, 101, 400, 448, 451, 472, 572, 668
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 4 cut(s) 130, 147, 263, 339
ScrFI CCNGG 4 cut(s) 12, 128, 387, 504
SfaNI GCATC 2 cut(s) 156, 462
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 1 cut(s) 339
SphI GCATGC 1 cut(s) 385
Sse9I AATT 2 cut(s) 490, 508
SsiI CCGC 9 cut(s) 72, 247, 399, 451, 471, 572, 608, 632, 704
SspMI CTAG 2 cut(s) 230, 318
StyD4I CCNGG 4 cut(s) 10, 126, 385, 502
StyI CCWWGG 2 cut(s) 229, 466
TaaI ACNGT 2 cut(s) 568, 664
TaiI ACGT 3 cut(s) 176, 366, 380
TaqI TCGA 3 cut(s) 16, 360, 521
TaqII GACCGA 1 cut(s) 356
TasI AATT 2 cut(s) 490, 508
TatI WGTACW 1 cut(s) 81
TauI GCSGC 4 cut(s) 402, 453, 474, 574
TfiI GAWTC 2 cut(s) 518, 541
TscAI CASTG 3 cut(s) 212, 573, 669
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 5 cut(s) 20, 29, 100, 447, 667
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 554
TspRI CASTG 3 cut(s) 212, 573, 669
Tth111I GACNNNGTC 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 339
XceI RCATGY 3 cut(s) 385, 614, 710
XmaJI CCTAGG 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 2 cut(s) 230, 318
ZraI GACGTC 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.