Rw3G013250

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0018175
Physical Location & Seq
Forward (+)
13147081 .. 13148903
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013250.1

Sequence Viewer

Length: 1494 bp
ATGCCTACTCCGGGTCGAGCAGCCGTCGATGCAATCCTCATATTCTGTCTCCTCCTCCTCAACAATGTTTCCGCCACCGAGTACACCTACATTGCCTCGCAGCAGGTGACGGCGCAGGACTTCCTCCAGGCCCACAACGAGGTCAGGGCCATGTTCAACGAGCCGATGCTAACGTGGGATGATAAGCTGGCTGACTACGCCCACCAGTGGGCTGAGAAGACCAGCGCCCTAGGGAAATGCGAGGAGCGGTACCACTCCAACGGCCCCTATGGCGAGAACCTCTTTTGGGGGTGGTCGTGGAACGACTACTTTAGCCCTAGAGACGCCGTCATGATGTGGACCGAGGAGAAAAACGACTTCGACGTCAAGTCGTTGACGTGCATGCCGGGGAAAATGTGCGGCCACTTCACGCAGGTCGTGTGGAGGAACTCCAAGAGAGTAGGGTGCAGCCGCATCAAGTGCGCCCAAGGCGGAGTGTTGATGATTTGTAATTATGATCCTCCTGGGAATTGGATTGATTCGAAACTTGGAGGTCTCATGAATCCCTTCAGCGAGGATAAGACGACAGTGCCGCCTGATTCGGCTGAACAAGTGTCGCAAGCAGTACCGCATGTTGTTCAGCCGAAAGTGCCGCAACCGGTGCCGGTTGATGGTCATCCGACAGTGCTGCCCGTTTCGGCTGAACAAGTGTCACAAGCAGTACCGGATGTTGTTCAGCCGAAAGTGCCGCAACCGGTGCCGGTTGATGGTCATCCGACAGTGCCGCCTGTTTCGGCTGAACAAGTGTCACAAGCAGTACAGCATGTTGTTCAGCCGAAAGTGCCGCAACCGGTGCCGGTTGATGGTCATCCTATAGTGCAGCACCCGGTGCCGGCTGACGGTCATCTGAAAGTGCCGCACCCAGTGCCGGCTGACGGTCATCCGACAGTGCCGCACCCGGTGCCGGCTGACGGTCATCGGACAGTGCCGCACCCGGTGCCGGTTGACGGTCATCGGACAGTGCCGCACCCGGTGCCGGTTGACGGTCATCCGACAGTGCCGCACCCGGTGCCTGTTGACGGTCATCCGACAGTGCCGCACCCGGTGCCGGTTGATGGTCATCGAACAGCGCCGCACCCTGTGCCGGCTGACGGGACAGTGCCGCACCCGGTGCCTGTTGACGGTCATCGGACAATGCCGCACCATCTGCCGGCTGAAGGTCATCCGACAGTGCCTCACCCTGTGCCGGTTGACGGTCATCCGACAGTGCCTCACCCAGCTGAACATCAACCGGCATTCTCCCTACCGCCGCTGTCTGGCGGAGAGTTGCCAAAGAAAGGAAGAGGAAGAAACAGAAGGGGTCAAGGGAGAGGAGGTCATCATCCTGGTCGTGCTAAACCTAACCAGAACCGTAGTCATCGTAACCGTAACCGTAACCATCACCACCTTAGCCCCCGCCAGCGCTATCTTAATCGCATTAGTCGACGTCGTCAACGTGATAATCGTAAACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

497

Amino Acids

54.63

Weight (kDa)

8.43

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 42 - 165 2e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018175)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0468031
rosa_laevigata RLG00000024457
rosa_multiflora Rmu_co8380321.1_g000001
rosa_roxburghii Rroxscaffold_6G00413160
rosa_rugosa Rorug03G0091600 Rorug03G0091700 Rorug03G0091800
rosa_samantha Rh3BG161600 Rh3CG161600 Rh3DG162100
rosa_wichuraiana Rw3G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
AasI GACNNNNNNGTC 1 cut(s) 362
AatII GACGTC 2 cut(s) 366, 1468
Acc36I ACCTGC 2 cut(s) 94, 403
Acc65I GGTACC 1 cut(s) 249
AccBSI CCGCTC 1 cut(s) 247
AccI GTMKAC 1 cut(s) 1462
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 2 cut(s) 532, 1215
AcyI GRCGYC 3 cut(s) 324, 363, 1465
AfaI GTAC 5 cut(s) 83, 251, 606, 702, 798
AfeI AGCGCT 1 cut(s) 1442
AgeI ACCGGT 3 cut(s) 637, 733, 829
AgsI TTSAA 1 cut(s) 157
AhdI GACNNNNNGTC 1 cut(s) 367
AjiI CACGTC 1 cut(s) 378
AjnI CCWGG 3 cut(s) 126, 502, 1363
AluBI AGCT 2 cut(s) 187, 1259
AluI AGCT 2 cut(s) 187, 1259
Alw26I GTCTC 3 cut(s) 53, 315, 539
AlwI GGATC 1 cut(s) 491
Aor51HI AGCGCT 1 cut(s) 1442
AoxI GGCC 4 cut(s) 129, 147, 262, 400
ApeKI GCWGC 5 cut(s) 20, 100, 447, 667, 859
AsiGI ACCGGT 3 cut(s) 637, 733, 829
Asp700I GAANNNNTTC 1 cut(s) 545
Asp718I GGTACC 1 cut(s) 249
AspA2I CCTAGG 1 cut(s) 229
AspLEI GCGC 5 cut(s) 115, 227, 464, 1111, 1443
AspS9I GGNCC 4 cut(s) 130, 147, 263, 339
AsuC2I CCSGG 9 cut(s) 12, 387, 866, 938, 974, 1010, 1046, 1082, 1148
AsuHPI GGTGA 4 cut(s) 118, 1208, 1244, 1412
AsuII TTCGAA 1 cut(s) 521
AvaII GGWCC 1 cut(s) 339
AvrII CCTAGG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 224
BbvI GCAGC 5 cut(s) 32, 112, 459, 654, 871
BccI CCATC 6 cut(s) 644, 740, 836, 1088, 1191, 1425
BceAI ACGGC 4 cut(s) 8, 126, 277, 311
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 3 cut(s) 128, 504, 1365
BcnI CCSGG 9 cut(s) 12, 387, 866, 938, 974, 1010, 1046, 1082, 1148
BcoDI GTCTC 3 cut(s) 53, 315, 539
BfaI CTAG 2 cut(s) 230, 318
BfmI CTRYAG 1 cut(s) 852
BfoI RGCGCY 3 cut(s) 228, 1112, 1444
BfuAI ACCTGC 2 cut(s) 94, 403
BglI GCCNNNNNGGC 1 cut(s) 270
BlnI CCTAGG 1 cut(s) 229
Bme18I GGWCC 1 cut(s) 339
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 378
BmgT120I GGNCC 4 cut(s) 130, 147, 263, 339
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 3 cut(s) 19, 156, 462
BmuI ACTGGG 1 cut(s) 896
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 1427
Bpu14I TTCGAA 1 cut(s) 521
BpuMI CCSGG 9 cut(s) 12, 387, 866, 938, 974, 1010, 1046, 1082, 1148
BsaBI GATNNNNATC 4 cut(s) 654, 750, 846, 1098
BsaHI GRCGYC 3 cut(s) 324, 363, 1465
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BsaWI WCCGGW 4 cut(s) 637, 703, 733, 829
BsaXI ACNNNNNCTCC 2 cut(s) 1339, 1369
Bse1I ACTGG 2 cut(s) 205, 902
Bse3DI GCAATG 1 cut(s) 90
Bse8I GATNNNNATC 4 cut(s) 654, 750, 846, 1098
BseBI CCWGG 3 cut(s) 128, 504, 1365
BseDI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BseJI GATNNNNATC 4 cut(s) 654, 750, 846, 1098
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 205, 902
BseRI GAGGAG 6 cut(s) 41, 44, 47, 257, 359, 1365
BseXI GCAGC 5 cut(s) 32, 112, 459, 654, 871
BseYI CCCAGC 1 cut(s) 1255
BsgI GTGCAG 2 cut(s) 466, 878
BshFI GGCC 4 cut(s) 131, 149, 264, 402
BshTI ACCGGT 3 cut(s) 637, 733, 829
BslFI GGGAC 1 cut(s) 1147
BsmAI GTCTC 3 cut(s) 53, 315, 539
BsmBI CGTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 1147
BsmI GAATGC 1 cut(s) 1274
BsnI GGCC 4 cut(s) 131, 149, 264, 402
Bso31I GGTCTC 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 521
Bsp143I GATC 1 cut(s) 496
BspANI GGCC 4 cut(s) 131, 149, 264, 402
BspCNI CTCAG 1 cut(s) 205
BspHI TCATGA 2 cut(s) 330, 537
BspMI ACCTGC 2 cut(s) 94, 403
BspPI GGATC 1 cut(s) 491
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 539
BsrBI CCGCTC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 2 cut(s) 205, 902
BssECI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BssMI GATC 1 cut(s) 496
BssNI GRCGYC 3 cut(s) 324, 363, 1465
BssT1I CCWWGG 2 cut(s) 229, 466
Bst2UI CCWGG 3 cut(s) 128, 504, 1365
Bst6I CTCTTC 1 cut(s) 1315
BstACI GRCGYC 3 cut(s) 324, 363, 1465
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 9 cut(s) 189, 383, 600, 873, 909, 945, 1125, 1191, 1439
BstDEI CTNAG 2 cut(s) 213, 1427
BstH2I RGCGCY 3 cut(s) 228, 1112, 1444
BstHHI GCGC 5 cut(s) 115, 227, 464, 1111, 1443
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 3 cut(s) 53, 315, 539
BstMBI GATC 1 cut(s) 496
BstNI CCWGG 3 cut(s) 128, 504, 1365
BstNSI RCATGY 3 cut(s) 385, 614, 806
BstSFI CTRYAG 1 cut(s) 852
BstV1I GCAGC 5 cut(s) 32, 112, 459, 654, 871
BstV2I GAAGAC 1 cut(s) 224
BsuRI GGCC 4 cut(s) 131, 149, 264, 402
BtrI CACGTC 1 cut(s) 378
BveI ACCTGC 2 cut(s) 94, 403
Cac8I GCNNGC 9 cut(s) 189, 383, 600, 873, 909, 945, 1125, 1191, 1439
CciI TCATGA 2 cut(s) 330, 537
CfoI GCGC 5 cut(s) 115, 227, 464, 1111, 1443
Cfr13I GGNCC 4 cut(s) 130, 147, 263, 339
CseI GACGC 1 cut(s) 332
Csp6I GTAC 5 cut(s) 82, 250, 605, 701, 797
CspAI ACCGGT 3 cut(s) 637, 733, 829
CviAII CATG 6 cut(s) 151, 331, 382, 538, 611, 803
CviQI GTAC 5 cut(s) 82, 250, 605, 701, 797
DdeI CTNAG 2 cut(s) 213, 1427
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
DrdI GACNNNNNNGTC 1 cut(s) 362
DriI GACNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 1315
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 1315
EciI GGCGGA 3 cut(s) 61, 486, 1314
Eco130I CCWWGG 2 cut(s) 229, 466
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 339
Eco47III AGCGCT 1 cut(s) 1442
Eco57I CTGAAG 2 cut(s) 532, 1215
EcoRII CCWGG 3 cut(s) 126, 502, 1363
EcoT14I CCWWGG 2 cut(s) 229, 466
ErhI CCWWGG 2 cut(s) 229, 466
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 6 cut(s) 154, 334, 385, 541, 614, 806
FaqI GGGAC 1 cut(s) 1147
FatI CATG 6 cut(s) 150, 330, 381, 537, 610, 802
FauI CCCGC 1 cut(s) 1442
FblI GTMKAC 1 cut(s) 1462
FspBI CTAG 2 cut(s) 230, 318
GlaI GCGC 5 cut(s) 114, 226, 463, 1110, 1442
GsaI CCCAGC 1 cut(s) 1259
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 3 cut(s) 228, 1112, 1444
HaeIII GGCC 4 cut(s) 131, 149, 264, 402
HgaI GACGC 1 cut(s) 332
HhaI GCGC 5 cut(s) 115, 227, 464, 1111, 1443
Hin1I GRCGYC 3 cut(s) 324, 363, 1465
Hin1II CATG 6 cut(s) 154, 334, 385, 541, 614, 806
Hin6I GCGC 5 cut(s) 113, 225, 462, 1109, 1441
HinP1I GCGC 5 cut(s) 113, 225, 462, 1109, 1441
HincII GTYRAC 8 cut(s) 375, 985, 1021, 1057, 1159, 1231, 1463, 1472
HindII GTYRAC 8 cut(s) 375, 985, 1021, 1057, 1159, 1231, 1463, 1472
HinfI GANTC 3 cut(s) 518, 541, 578
HphI GGTGA 4 cut(s) 118, 1208, 1244, 1412
Hpy188III TCNNGA 2 cut(s) 331, 538
Hpy99I CGWCG 4 cut(s) 29, 365, 1467, 1470
HpyAV CCTTC 3 cut(s) 556, 1190, 1329
HpyCH4IV ACGT 5 cut(s) 173, 363, 377, 1465, 1474
HpyCH4V TGCA 4 cut(s) 32, 381, 447, 859
HpyF3I CTNAG 2 cut(s) 213, 1427
HpySE526I ACGT 5 cut(s) 173, 363, 377, 1465, 1474
Hsp92I GRCGYC 3 cut(s) 324, 363, 1465
Hsp92II CATG 6 cut(s) 154, 334, 385, 541, 614, 806
HspAI GCGC 5 cut(s) 113, 225, 462, 1109, 1441
KpnI GGTACC 1 cut(s) 253
KroI GCCGGC 5 cut(s) 871, 907, 943, 1123, 1189
KroNI GCCGGC 5 cut(s) 873, 909, 945, 1125, 1191
Kzo9I GATC 1 cut(s) 496
LmnI GCTCC 1 cut(s) 244
Lsp1109I GCAGC 5 cut(s) 32, 112, 459, 654, 871
LweI GCATC 3 cut(s) 19, 156, 462
MaeI CTAG 2 cut(s) 230, 318
MaeII ACGT 5 cut(s) 173, 363, 377, 1465, 1474
MaeIII GTNAC 6 cut(s) 106, 690, 786, 1400, 1406, 1412
MalI GATC 1 cut(s) 498
MbiI CCGCTC 1 cut(s) 247
MboI GATC 1 cut(s) 496
MboII GAAGA 3 cut(s) 229, 1332, 1338
MluCI AATT 2 cut(s) 490, 508
MmeI TCCRAC 8 cut(s) 282, 683, 779, 947, 1055, 1091, 1229, 1265
MroNI GCCGGC 5 cut(s) 871, 907, 943, 1123, 1189
MroXI GAANNNNTTC 1 cut(s) 545
MseI TTAA 1 cut(s) 1449
MspA1I CMGCKG 2 cut(s) 1259, 1291
Mva1269I GAATGC 1 cut(s) 1274
MvaI CCWGG 3 cut(s) 128, 504, 1365
NaeI GCCGGC 5 cut(s) 873, 909, 945, 1125, 1191
NciI CCSGG 9 cut(s) 12, 387, 866, 938, 974, 1010, 1046, 1082, 1148
NdeII GATC 1 cut(s) 496
NgoMIV GCCGGC 5 cut(s) 871, 907, 943, 1123, 1189
NlaIII CATG 6 cut(s) 154, 334, 385, 541, 614, 806
NmuCI GTSAC 3 cut(s) 106, 690, 786
NspI RCATGY 3 cut(s) 385, 614, 806
NspV TTCGAA 1 cut(s) 521
PaeI GCATGC 1 cut(s) 385
PagI TCATGA 2 cut(s) 330, 537
PaqCI CACCTGC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 4 cut(s) 360, 1459, 1471, 1480
PctI GAATGC 1 cut(s) 1274
PdiI GCCGGC 5 cut(s) 873, 909, 945, 1125, 1191
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 3 cut(s) 518, 541, 578
PflFI GACNNNGTC 2 cut(s) 326, 1467
PinAI ACCGGT 3 cut(s) 637, 733, 829
Psp6I CCWGG 3 cut(s) 126, 502, 1363
PspFI CCCAGC 1 cut(s) 1255
PspGI CCWGG 3 cut(s) 126, 502, 1363
PspPI GGNCC 4 cut(s) 130, 147, 263, 339
PsyI GACNNNGTC 2 cut(s) 326, 1467
PvuII CAGCTG 1 cut(s) 1259
RsaI GTAC 5 cut(s) 83, 251, 606, 702, 798
RsaNI GTAC 5 cut(s) 82, 250, 605, 701, 797
SalI GTCGAC 1 cut(s) 1461
SaqAI TTAA 1 cut(s) 1449
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 4 cut(s) 130, 147, 263, 339
SfaNI GCATC 3 cut(s) 19, 156, 462
SfcI CTRYAG 1 cut(s) 852
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 1 cut(s) 339
SphI GCATGC 1 cut(s) 385
Sse9I AATT 2 cut(s) 490, 508
SspMI CTAG 2 cut(s) 230, 318
StyI CCWWGG 2 cut(s) 229, 466
TaiI ACGT 5 cut(s) 176, 366, 380, 1468, 1477
TaqI TCGA 6 cut(s) 16, 27, 360, 521, 1102, 1462
TaqII GACCGA 1 cut(s) 356
TasI AATT 2 cut(s) 490, 508
TatI WGTACW 2 cut(s) 81, 796
TfiI GAWTC 3 cut(s) 518, 541, 578
Tru1I TTAA 1 cut(s) 1449
Tru9I TTAA 1 cut(s) 1449
TseFI GTSAC 3 cut(s) 106, 690, 786
TseI GCWGC 5 cut(s) 20, 100, 447, 667, 859
Tsp45I GTSAC 3 cut(s) 106, 690, 786
TspDTI ATGAA 1 cut(s) 554
Tth111I GACNNNGTC 2 cut(s) 326, 1467
VpaK11BI GGWCC 1 cut(s) 339
XceI RCATGY 3 cut(s) 385, 614, 806
XmaJI CCTAGG 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 1462
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 2 cut(s) 230, 318
ZraI GACGTC 2 cut(s) 364, 1466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.