Rh3BG161600

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0018175
Physical Location & Seq
Forward (+)
13956536 .. 13958205
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG161600.1

Sequence Viewer

Length: 1566 bp
ATGCCTACTCCGGGTCGAGCAGCCGTCGATGCAATCCTCATATTCTGTCTCCTCCTCCTCAACAATGTTTCCGCCACCGAGTACACCTACATTGCCTCGCAGCAGGTGACGGCGCAGGACTTCCTCCAGGCCCACAACGAGGTCAGGGCCATGTTCAACGAGCCGATGCTAACGTGGGATGATAAGCTGGCTGACTACGCCCACCAGTGGGCTGAGAAGACCAGCGCCCTAGGGAAATGCGAGGAGCGGTACCACTCGAACGGCCCCTATGGCGAGAACCTCTTTTGGGGGTGGTCGTGGAACGACTACTTTAGCCCTAGAGACGCCGTCATGATGTGGACCGAGGAGAAAAACGACTTCGACGTCAAGTCGTTGACGTGCATGCCGGGGAAAATGTGCGGCCACTTCACGCAGGTCGTGTGGAGGAACTCCAAGAGAGTAGGGTGCAGCCGCATCAAGTGCGCCCAAGGCGGAGTGTTGATGATTTGTAATTATGATCCTCCTGGGAATTGGATTGATTCGAAACTTGGAGGTCTCATGAATCCCTTCAGCGAGGATAAGACGACAGTGCCGCCTGATTCGGCTGAACAAGTGTCGCAAGCAGTACCGCATGTTGTTCAGCCGAAAGTGCCGCAACCGGTGCCGGTTGATGGTCATCTGACAGTGCTGCCCGTTTCGGCTGAACAAGTGTCACAAGCAGTACCGGATGTTGTTCAGCCGAAAGTGCCGCAACCGGTGCCGGTTGATGGTCATCCGACAGTGCTGCCCGTTTCGGCTGAACAAGTGTCACAAGCAGTACCGGATGTTGTTCAGCCGAAAGTGCCGCAACCGGTGCCGGTTGATGGTCATCCGACAGTGCCGCCTGTTTCGGCTGAACAAGTGTCACAAGCAGTACCGCATGTTGTTCAGCCGAAAGTGCCGCAACCGGTGCCGGTTGATGGTCATCCTATAGTGCAGCACCCGGTGCCGGCTGACGGTCATCTGAAAGTGCCGCACCCAGTGCAGGCTGACGGTCATCCGACAGTGCCGCACCCGGTGCCTATTGACGGTCATCCGACAGTGCCGCACCCGGTGCCGGTTGACGGTCATCCGACAGTGCCGCACCCGGTGCCGGTTGACGGTCATCGGACAGTGCCGCACCCGGTGCCTGTTGACGGTCATCCGACAGTGCCGCACCCGGTGCCGGTTGACGGTCATCCGACAGTGCCGCACCCGGTGCCGGTTGACGGTCATCGGACAGTGCCGCACCTGGTGCCTGTTGACGGTCATCCGACAGTGCAGCACCCGGTGCCAGTTGATGGTCATCGAACAGCGCCGCACCCTGTGCCGGCTGACAGGACAGTGCCGCTCCCGGTGCCTGTTGACGGTTATCGGACAATGCCGCACCATGTGCCGGCTGAAGGTCATCCGACAGTGCCTCACCCTGTGCCGGTTGACGGTCATCCGACAGTGCCTCACCCAGCTGAACATCAACCGGCATTCTCCCTACCGCCGCTGTCTGGCGGAGAGTTGCCAAAGAAAGGAAGAGGAAGAAACAGAAGGGTACATCCTGCACACCACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

521

Amino Acids

56.46

Weight (kDa)

6.23

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 42 - 165 2.2e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018175)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0468031
rosa_laevigata RLG00000024457
rosa_multiflora Rmu_co8380321.1_g000001
rosa_roxburghii Rroxscaffold_6G00413160
rosa_rugosa Rorug03G0091600 Rorug03G0091700 Rorug03G0091800
rosa_samantha Rh3BG161600 Rh3CG161600 Rh3DG162100
rosa_wichuraiana Rw3G013250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 94
AasI GACNNNNNNGTC 1 cut(s) 362
AatII GACGTC 1 cut(s) 366
Acc36I ACCTGC 2 cut(s) 94, 403
Acc65I GGTACC 1 cut(s) 249
AccB7I CCANNNNNTGG 1 cut(s) 1298
AccBSI CCGCTC 2 cut(s) 247, 1348
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 2 cut(s) 532, 1419
AcyI GRCGYC 2 cut(s) 324, 363
AfaI GTAC 7 cut(s) 83, 251, 606, 702, 798, 894, 1545
AgeI ACCGGT 4 cut(s) 637, 733, 829, 925
AgsI TTSAA 1 cut(s) 157
AhdI GACNNNNNGTC 1 cut(s) 367
AjiI CACGTC 1 cut(s) 378
AjnI CCWGG 3 cut(s) 126, 502, 1248
AluBI AGCT 2 cut(s) 187, 1463
AluI AGCT 2 cut(s) 187, 1463
Alw26I GTCTC 3 cut(s) 53, 315, 539
AlwI GGATC 1 cut(s) 491
AoxI GGCC 4 cut(s) 129, 147, 262, 400
ApeKI GCWGC 7 cut(s) 20, 100, 447, 667, 763, 955, 1279
AsiGI ACCGGT 4 cut(s) 637, 733, 829, 925
Asp700I GAANNNNTTC 1 cut(s) 545
Asp718I GGTACC 1 cut(s) 249
AspA2I CCTAGG 1 cut(s) 229
AspLEI GCGC 4 cut(s) 115, 227, 464, 1315
AspS9I GGNCC 4 cut(s) 130, 147, 263, 339
AsuHPI GGTGA 3 cut(s) 118, 1412, 1448
AsuII TTCGAA 1 cut(s) 521
AvaII GGWCC 1 cut(s) 339
AvrII CCTAGG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 224
BbvI GCAGC 7 cut(s) 32, 112, 459, 654, 750, 967, 1291
BccI CCATC 5 cut(s) 644, 740, 836, 932, 1292
BceAI ACGGC 4 cut(s) 8, 126, 277, 311
BcgI CGANNNNNNTGC 2 cut(s) 745, 779
BciT130I CCWGG 3 cut(s) 128, 504, 1250
BcoDI GTCTC 3 cut(s) 53, 315, 539
BfaI CTAG 2 cut(s) 230, 318
BfmI CTRYAG 1 cut(s) 948
BfoI RGCGCY 2 cut(s) 228, 1316
BfuAI ACCTGC 2 cut(s) 94, 403
BglI GCCNNNNNGGC 1 cut(s) 270
BlnI CCTAGG 1 cut(s) 229
Bme18I GGWCC 1 cut(s) 339
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 378
BmgT120I GGNCC 4 cut(s) 130, 147, 263, 339
BmrI ACTGGG 1 cut(s) 992
BmsI GCATC 3 cut(s) 19, 156, 462
BmuI ACTGGG 1 cut(s) 992
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 521
BsaBI GATNNNNATC 5 cut(s) 654, 750, 846, 942, 1302
BsaHI GRCGYC 2 cut(s) 324, 363
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BsaWI WCCGGW 6 cut(s) 637, 703, 733, 799, 829, 925
Bse1I ACTGG 3 cut(s) 205, 998, 1292
Bse3DI GCAATG 1 cut(s) 90
Bse8I GATNNNNATC 5 cut(s) 654, 750, 846, 942, 1302
BseBI CCWGG 3 cut(s) 128, 504, 1250
BseDI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BseJI GATNNNNATC 5 cut(s) 654, 750, 846, 942, 1302
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 3 cut(s) 205, 998, 1292
BseRI GAGGAG 5 cut(s) 41, 44, 47, 257, 359
BseXI GCAGC 7 cut(s) 32, 112, 459, 654, 750, 967, 1291
BseYI CCCAGC 1 cut(s) 1459
BsgI GTGCAG 5 cut(s) 466, 974, 1022, 1298, 1536
BshFI GGCC 4 cut(s) 131, 149, 264, 402
BshTI ACCGGT 4 cut(s) 637, 733, 829, 925
BsmAI GTCTC 3 cut(s) 53, 315, 539
BsmBI CGTCTC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 1478
BsnI GGCC 4 cut(s) 131, 149, 264, 402
Bso31I GGTCTC 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 521
Bsp143I GATC 1 cut(s) 496
BspANI GGCC 4 cut(s) 131, 149, 264, 402
BspCNI CTCAG 1 cut(s) 205
BspHI TCATGA 2 cut(s) 330, 537
BspMI ACCTGC 2 cut(s) 94, 403
BspPI GGATC 1 cut(s) 491
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 539
BsrBI CCGCTC 2 cut(s) 247, 1348
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 3 cut(s) 205, 998, 1292
BssECI CCNNGG 5 cut(s) 229, 342, 386, 466, 503
BssMI GATC 1 cut(s) 496
BssNI GRCGYC 2 cut(s) 324, 363
BssT1I CCWWGG 2 cut(s) 229, 466
Bst2UI CCWGG 3 cut(s) 128, 504, 1250
Bst6I CTCTTC 1 cut(s) 1519
BstACI GRCGYC 2 cut(s) 324, 363
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 7 cut(s) 189, 383, 600, 969, 1005, 1329, 1395
BstDEI CTNAG 1 cut(s) 213
BstH2I RGCGCY 2 cut(s) 228, 1316
BstHHI GCGC 4 cut(s) 115, 227, 464, 1315
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 3 cut(s) 53, 315, 539
BstMBI GATC 1 cut(s) 496
BstNI CCWGG 3 cut(s) 128, 504, 1250
BstNSI RCATGY 3 cut(s) 385, 614, 902
BstSFI CTRYAG 1 cut(s) 948
BstV1I GCAGC 7 cut(s) 32, 112, 459, 654, 750, 967, 1291
BstV2I GAAGAC 1 cut(s) 224
BsuRI GGCC 4 cut(s) 131, 149, 264, 402
BtrI CACGTC 1 cut(s) 378
BveI ACCTGC 2 cut(s) 94, 403
Cac8I GCNNGC 7 cut(s) 189, 383, 600, 969, 1005, 1329, 1395
CciI TCATGA 2 cut(s) 330, 537
CfoI GCGC 4 cut(s) 115, 227, 464, 1315
Cfr13I GGNCC 4 cut(s) 130, 147, 263, 339
CseI GACGC 1 cut(s) 332
CsiI ACCWGGT 1 cut(s) 1248
Csp6I GTAC 7 cut(s) 82, 250, 605, 701, 797, 893, 1544
CspAI ACCGGT 4 cut(s) 637, 733, 829, 925
CviAII CATG 7 cut(s) 151, 331, 382, 538, 611, 899, 1388
CviQI GTAC 7 cut(s) 82, 250, 605, 701, 797, 893, 1544
DdeI CTNAG 1 cut(s) 213
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
DrdI GACNNNNNNGTC 1 cut(s) 362
DriI GACNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 1519
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 1519
EciI GGCGGA 3 cut(s) 61, 486, 1518
Eco130I CCWWGG 2 cut(s) 229, 466
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 339
Eco57I CTGAAG 2 cut(s) 532, 1419
EcoRII CCWGG 3 cut(s) 126, 502, 1248
EcoT14I CCWWGG 2 cut(s) 229, 466
ErhI CCWWGG 2 cut(s) 229, 466
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 7 cut(s) 154, 334, 385, 541, 614, 902, 1391
FatI CATG 7 cut(s) 150, 330, 381, 537, 610, 898, 1387
FspBI CTAG 2 cut(s) 230, 318
GlaI GCGC 4 cut(s) 114, 226, 463, 1314
GsaI CCCAGC 1 cut(s) 1463
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 2 cut(s) 228, 1316
HaeIII GGCC 4 cut(s) 131, 149, 264, 402
HgaI GACGC 1 cut(s) 332
HhaI GCGC 4 cut(s) 115, 227, 464, 1315
Hin1I GRCGYC 2 cut(s) 324, 363
Hin1II CATG 7 cut(s) 154, 334, 385, 541, 614, 902, 1391
Hin6I GCGC 4 cut(s) 113, 225, 462, 1313
HinP1I GCGC 4 cut(s) 113, 225, 462, 1313
HincII GTYRAC 9 cut(s) 375, 1081, 1117, 1153, 1189, 1225, 1261, 1363, 1435
HindII GTYRAC 9 cut(s) 375, 1081, 1117, 1153, 1189, 1225, 1261, 1363, 1435
HinfI GANTC 3 cut(s) 518, 541, 578
HphI GGTGA 3 cut(s) 118, 1412, 1448
Hpy188III TCNNGA 2 cut(s) 331, 538
Hpy99I CGWCG 2 cut(s) 29, 365
HpyAV CCTTC 3 cut(s) 556, 1394, 1533
HpyCH4IV ACGT 3 cut(s) 173, 363, 377
HpyCH4V TGCA 7 cut(s) 32, 381, 447, 955, 1003, 1279, 1553
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 3 cut(s) 173, 363, 377
Hsp92I GRCGYC 2 cut(s) 324, 363
Hsp92II CATG 7 cut(s) 154, 334, 385, 541, 614, 902, 1391
HspAI GCGC 4 cut(s) 113, 225, 462, 1313
KpnI GGTACC 1 cut(s) 253
KroI GCCGGC 3 cut(s) 967, 1327, 1393
KroNI GCCGGC 3 cut(s) 969, 1329, 1395
Kzo9I GATC 1 cut(s) 496
LmnI GCTCC 2 cut(s) 244, 1353
Lsp1109I GCAGC 7 cut(s) 32, 112, 459, 654, 750, 967, 1291
LweI GCATC 3 cut(s) 19, 156, 462
MabI ACCWGGT 1 cut(s) 1248
MaeI CTAG 2 cut(s) 230, 318
MaeII ACGT 3 cut(s) 173, 363, 377
MaeIII GTNAC 4 cut(s) 106, 690, 786, 882
MalI GATC 1 cut(s) 498
MbiI CCGCTC 2 cut(s) 247, 1348
MboI GATC 1 cut(s) 496
MboII GAAGA 3 cut(s) 229, 1536, 1542
MluCI AATT 2 cut(s) 490, 508
MroNI GCCGGC 3 cut(s) 967, 1327, 1393
MroXI GAANNNNTTC 1 cut(s) 545
MspA1I CMGCKG 2 cut(s) 1463, 1495
Mva1269I GAATGC 1 cut(s) 1478
MvaI CCWGG 3 cut(s) 128, 504, 1250
NaeI GCCGGC 3 cut(s) 969, 1329, 1395
NdeII GATC 1 cut(s) 496
NgoMIV GCCGGC 3 cut(s) 967, 1327, 1393
NlaIII CATG 7 cut(s) 154, 334, 385, 541, 614, 902, 1391
NmuCI GTSAC 4 cut(s) 106, 690, 786, 882
NspI RCATGY 3 cut(s) 385, 614, 902
NspV TTCGAA 1 cut(s) 521
PaeI GCATGC 1 cut(s) 385
PagI TCATGA 2 cut(s) 330, 537
PaqCI CACCTGC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 1478
PdiI GCCGGC 3 cut(s) 969, 1329, 1395
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 3 cut(s) 518, 541, 578
PflFI GACNNNGTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 1298
PinAI ACCGGT 4 cut(s) 637, 733, 829, 925
Psp6I CCWGG 3 cut(s) 126, 502, 1248
PspFI CCCAGC 1 cut(s) 1459
PspGI CCWGG 3 cut(s) 126, 502, 1248
PspPI GGNCC 4 cut(s) 130, 147, 263, 339
PsyI GACNNNGTC 1 cut(s) 326
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 7 cut(s) 83, 251, 606, 702, 798, 894, 1545
RsaNI GTAC 7 cut(s) 82, 250, 605, 701, 797, 893, 1544
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 4 cut(s) 130, 147, 263, 339
SexAI ACCWGGT 1 cut(s) 1248
SfaNI GCATC 3 cut(s) 19, 156, 462
SfcI CTRYAG 1 cut(s) 948
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 1 cut(s) 339
SphI GCATGC 1 cut(s) 385
Sse9I AATT 2 cut(s) 490, 508
SspMI CTAG 2 cut(s) 230, 318
StyI CCWWGG 2 cut(s) 229, 466
TaiI ACGT 3 cut(s) 176, 366, 380
TaqI TCGA 6 cut(s) 16, 27, 257, 360, 521, 1306
TaqII GACCGA 1 cut(s) 356
TasI AATT 2 cut(s) 490, 508
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 3 cut(s) 518, 541, 578
TseFI GTSAC 4 cut(s) 106, 690, 786, 882
TseI GCWGC 7 cut(s) 20, 100, 447, 667, 763, 955, 1279
Tsp45I GTSAC 4 cut(s) 106, 690, 786, 882
TspDTI ATGAA 1 cut(s) 554
Tth111I GACNNNGTC 1 cut(s) 326
Van91I CCANNNNNTGG 1 cut(s) 1298
VpaK11BI GGWCC 1 cut(s) 339
XceI RCATGY 3 cut(s) 385, 614, 902
XmaJI CCTAGG 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 2 cut(s) 230, 318
ZraI GACGTC 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.