RLG00000024535

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0019145
Physical Location & Seq
Forward (+)
36802776 .. 36805715
2940 bp
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UTR
Exon/CDS
Intron
RLM00000024535

Sequence Viewer

Length: 327 bp
ATGCATGCAAGGGAGACGGTGAACAATCAAAGTACGAAACGACTATTGGGAAGTCTATACCACAATGTACAAACAACAGGTAATACGGCGCCGTTTGATCGTCACTCACGCCAGATTTTCTATAAACGTAGACCCATCGCACTCGTTACCCTCCTCGACATACAGTTACACAGAGCTTCGACTTCTGCCATTGACTCAGACTACTCGCCTCCAAAAACTATGTTTGACGATCAGGACCTGGGCTTCTTTGCCAATTTCCTTGGCATCTTCATCTTTGTTTTGGTAATCGCGTATCATTATGTGATGGCTGACCCAAAATATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ost4

Protein Analysis

109

Amino Acids

12.47

Weight (kDa)

8.08

Isoelectric Point (pI)

57.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ost4 PF10215 74 - 106 1.6e-13 Oligosaccaryltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09455 AT3G09455 AT5G02502
malus_domestica MD04G1125200.v1.1
prunus_persica Prupe.6G252200_v2.0.a1
rosa_laevigata RLG00000024535
rosa_samantha Rh3BG153800 Rh3DG154900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 130
AccII CGCG 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 2 cut(s) 34, 69
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 1 cut(s) 176
AluI AGCT 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 8
AlwNI CAGNNNCTG 1 cut(s) 238
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 31
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 88
BccI CCATC 2 cut(s) 143, 298
BceAI ACGGC 2 cut(s) 76, 102
BciT130I CCWGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 8
BfoI RGCGCY 1 cut(s) 92
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 238, 259
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 238, 259
BseMII CTCAG 1 cut(s) 210
BseRI GAGGAG 1 cut(s) 143
Bsh1236I CGCG 1 cut(s) 290
BshNI GGYRCC 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 8
BsmBI CGTCTC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 67
Bsp143I GATC 2 cut(s) 97, 229
BspCNI CTCAG 1 cut(s) 209
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 88
BsrGI TGTACA 1 cut(s) 67
BssECI CCNNGG 2 cut(s) 238, 259
BssMI GATC 2 cut(s) 97, 229
BssNI GRCGYC 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 2 cut(s) 19, 165
BstACI GRCGYC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 2 cut(s) 100, 232
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 2 cut(s) 97, 229
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 237
BstUI CGCG 1 cut(s) 290
BtgZI GCGATG 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 238
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 235
Csp6I GTAC 2 cut(s) 33, 68
CviAII CATG 1 cut(s) 5
CviJI RGCY 3 cut(s) 176, 243, 308
CviKI_1 RGCY 3 cut(s) 176, 243, 308
CviQI GTAC 2 cut(s) 33, 68
DdeI CTNAG 1 cut(s) 196
DinI GGCGCC 1 cut(s) 90
DpnI GATC 2 cut(s) 99, 231
DpnII GATC 2 cut(s) 97, 229
Eco130I CCWWGG 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 259
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 90
EheI GGCGCC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 259
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 1 cut(s) 8
FaiI YATR 7 cut(s) 6, 58, 123, 161, 221, 300, 321
FatI CATG 1 cut(s) 4
FblI GTMKAC 1 cut(s) 130
GlaI GCGC 1 cut(s) 90
HaeII RGCGCY 1 cut(s) 92
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 1 cut(s) 8
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HinfI GANTC 1 cut(s) 194
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 22, 131
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 2 cut(s) 22, 131
HpyCH4III ACNGT 2 cut(s) 19, 165
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 2 cut(s) 4, 8
HpyF3I CTNAG 1 cut(s) 196
HpySE526I ACGT 1 cut(s) 127
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 1 cut(s) 8
HspAI GCGC 1 cut(s) 89
KasI GGCGCC 1 cut(s) 88
Kzo9I GATC 2 cut(s) 97, 229
LpnPI CCDG 5 cut(s) 63, 125, 218, 224, 251
LweI GCATC 1 cut(s) 273
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 3 cut(s) 101, 145, 165
MalI GATC 2 cut(s) 99, 231
MboI GATC 2 cut(s) 97, 229
MboII GAAGA 1 cut(s) 259
MluCI AATT 1 cut(s) 253
Mly113I GGCGCC 1 cut(s) 89
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 3 cut(s) 161, 164, 219
Mph1103I ATGCAT 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 290
NarI GGCGCC 1 cut(s) 89
NdeII GATC 2 cut(s) 97, 229
NlaIII CATG 1 cut(s) 8
NlaIV GGNNCC 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 101
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PleI GAGTC 1 cut(s) 188
PluTI GGCGCC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 188
PpuMI RGGWCCY 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 238
RsaI GTAC 2 cut(s) 34, 69
RsaNI GTAC 2 cut(s) 33, 68
Sau3AI GATC 2 cut(s) 97, 229
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 1 cut(s) 239
SetI ASST 4 cut(s) 82, 130, 178, 240
SfaNI GCATC 1 cut(s) 273
SfoI GGCGCC 1 cut(s) 90
SinI GGWCC 1 cut(s) 235
SphI GCATGC 1 cut(s) 8
Sse9I AATT 1 cut(s) 253
SspDI GGCGCC 1 cut(s) 88
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 19, 165
TaiI ACGT 1 cut(s) 130
TaqI TCGA 2 cut(s) 156, 179
TasI AATT 1 cut(s) 253
TatI WGTACW 1 cut(s) 67
TseFI GTSAC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 235
XceI RCATGY 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 130
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.