RLG00000025159

dna binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017503
Physical Location & Seq
Forward (+)
42624982 .. 42625839
858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025159

Sequence Viewer

Length: 858 bp
ATGAAGAACCCTAATTCCGACGACACGGCTCCCAATCCAAAACCGAACAACCACCATGGCGCCGCCAATGTGCCCCTGAGAGTAGTGGCACCGGGATCAGCCTCGGCCGGAGGAGGAGGAGGAGGAGCAGCCGGCGGCGGAGTAGTAGAGGAGTGCAGCAACTGCGGGACCAAGAAGGGGTGGGTCCTACACAATGTCCGTTACCGAGCCACCCTCCGCCGCTTCTGCACCTCCTGCGTCCTCCGCCTCCACCCGGCCAGCTTCTGCCCCATCTGCTTCCTCTTCCAGGACCACAACAATTCCCAAAATGCCCCTCCTCCCAACCTCATCTCCTGCTCCAAGTGCCCCTCCCTCTCCCACTCCGACTGCCTCCCTCCCTACGCCGCCTTCTCCCCCTCCCCGTTCCTCTGCCCGCCCTGCGCCTCCGCCAACCCCAAATTCTCCTTCTTCGAGCTCGACGACCGCAACAAGGTCCTCGACAAGCGCCTCGCCGCCGTGCTGCTCTGCGCCGCCAGCATCGCCGCCTCGTCCATGTCCAAAGCCGCTGCCATCTCACGCGCCGAGGCCGAGCGGCGCGTGAGGGAGGCCGCCTTGGCTCGGAAGCGCGCTCGCGAGGCCTTGGACCGCTTCGTGCTCCTCTCCTCCGGCTCCTCCTTCCAACCCAGACCCGCCGTCAAGGCGGCGGCTCGCGCAACCGAGGTCTCTGGCTCCTCCAATGGCGGCAAAGAACCCTCCAAGAACAAGACCACCACCCCAGTTGCATTGACGAACAAAGCTCATCACTCTAATGGGGGCTTGCATCATAACCAAGAAGAAAAGGGTGAAGAGCAGAAGGTGAAGTTGGAGCTGAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

30.17

Weight (kDa)

9.41

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09520
fragaria_vesca FvH4_6g08440
rosa_chinensis RchiOBHm_Chr3g0458411
rosa_laevigata RLG00000025159
rosa_multiflora Rmu_sc0013809.1_g000002
rosa_roxburghii Rroxscaffold_6G00421090
rosa_rugosa Rorug03G0027700
rosa_samantha Rh3AG087500 Rh3BG090300 Rh3CG090600 Rh3DG091200
rosa_wichuraiana Rw3G007430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 59, 88
AccBSI CCGCTC 1 cut(s) 571
AccII CGCG 5 cut(s) 558, 576, 606, 612, 690
AclWI GGATC 1 cut(s) 103
AcoI YGGCCR 2 cut(s) 105, 255
AcsI RAATTY 1 cut(s) 437
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 5 cut(s) 177, 253, 286, 469, 597
AhdI GACNNNNNGTC 1 cut(s) 671
AjnI CCWGG 1 cut(s) 285
AjuI GAANNNNNNNTTGG 6 cut(s) 422, 428, 454, 460, 824, 856
AloI GAACNNNNNNTCC 1 cut(s) 31
AluBI AGCT 4 cut(s) 261, 454, 776, 847
AluI AGCT 4 cut(s) 261, 454, 776, 847
Alw21I GWGCWC 2 cut(s) 456, 636
Alw26I GTCTC 1 cut(s) 706
AlwI GGATC 1 cut(s) 103
AlwNI CAGNNNCTG 2 cut(s) 162, 264
AoxI GGCC 5 cut(s) 105, 255, 564, 585, 615
ApeKI GCWGC 4 cut(s) 128, 156, 499, 545
ApoI RAATTY 1 cut(s) 437
AspLEI GCGC 9 cut(s) 62, 422, 486, 509, 560, 576, 606, 608, 692
AspS9I GGNCC 5 cut(s) 168, 184, 289, 472, 622
AsuC2I CCSGG 2 cut(s) 93, 254
AsuHPI GGTGA 2 cut(s) 833, 847
AvaII GGWCC 5 cut(s) 168, 184, 289, 472, 622
BaeGI GKGCMC 2 cut(s) 75, 347
BanI GGYRCC 2 cut(s) 59, 88
BanII GRGCYC 1 cut(s) 456
Bbv12I GWGCWC 2 cut(s) 456, 636
BbvI GCAGC 4 cut(s) 140, 168, 486, 532
BccI CCATC 2 cut(s) 278, 557
BceAI ACGGC 3 cut(s) 42, 479, 656
BciT130I CCWGG 1 cut(s) 287
BcnI CCSGG 2 cut(s) 93, 254
BcoDI GTCTC 1 cut(s) 706
BfoI RGCGCY 2 cut(s) 63, 487
BglI GCCNNNNNGGC 2 cut(s) 593, 677
Bme1390I CCNGG 3 cut(s) 93, 254, 287
Bme18I GGWCC 5 cut(s) 168, 184, 289, 472, 622
BmeRI GACNNNNNGTC 1 cut(s) 671
BmgT120I GGNCC 5 cut(s) 168, 184, 289, 472, 622
BmiI GGNNCC 7 cut(s) 30, 61, 90, 169, 185, 649, 709
BmrFI CCNGG 3 cut(s) 93, 254, 287
BmrI ACTGGG 1 cut(s) 749
BmsI GCATC 2 cut(s) 525, 808
BmuI ACTGGG 1 cut(s) 749
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpuMI CCSGG 2 cut(s) 93, 254
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 706
BsaJI CCNNGG 6 cut(s) 55, 102, 561, 591, 618, 696
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 253, 286, 469, 597
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 755
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 6 cut(s) 55, 102, 561, 591, 618, 696
BseLI CCNNNNNNNGG 5 cut(s) 177, 253, 286, 469, 597
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 1 cut(s) 755
BsePI GCGCGC 1 cut(s) 604
BseSI GKGCMC 2 cut(s) 75, 347
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 4 cut(s) 140, 168, 486, 532
BsgI GTGCAG 2 cut(s) 175, 211
Bsh1236I CGCG 5 cut(s) 558, 576, 606, 612, 690
Bsh1285I CGRYCG 2 cut(s) 108, 463
BshFI GGCC 5 cut(s) 107, 257, 566, 587, 617
BshNI GGYRCC 2 cut(s) 59, 88
BsiEI CGRYCG 2 cut(s) 108, 463
BsiHKAI GWGCWC 2 cut(s) 456, 636
BsiSI CCGG 5 cut(s) 92, 108, 132, 254, 645
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 5 cut(s) 177, 253, 286, 469, 597
BsmAI GTCTC 1 cut(s) 706
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 5 cut(s) 107, 257, 566, 587, 617
Bso31I GGTCTC 1 cut(s) 706
Bsp1286I GDGCHC 4 cut(s) 75, 347, 456, 636
Bsp143I GATC 1 cut(s) 95
Bsp19I CCATGG 1 cut(s) 55
Bsp68I TCGCGA 1 cut(s) 612
BspANI GGCC 5 cut(s) 107, 257, 566, 587, 617
BspCNI CTCAG 1 cut(s) 69
BspFNI CGCG 5 cut(s) 558, 576, 606, 612, 690
BspLI GGNNCC 7 cut(s) 30, 61, 90, 169, 185, 649, 709
BspPI GGATC 1 cut(s) 103
BspQI GCTCTTC 1 cut(s) 819
BspT107I GGYRCC 2 cut(s) 59, 88
BspTNI GGTCTC 1 cut(s) 706
BsrBI CCGCTC 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 1 cut(s) 755
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 6 cut(s) 55, 102, 561, 591, 618, 696
BssHII GCGCGC 1 cut(s) 604
BssMI GATC 1 cut(s) 95
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 3 cut(s) 55, 591, 618
Bst2UI CCWGG 1 cut(s) 287
Bst6I CTCTTC 2 cut(s) 287, 819
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 2 cut(s) 162, 234
BstC8I GCNNGC 8 cut(s) 133, 259, 413, 514, 606, 610, 688, 797
BstDEI CTNAG 1 cut(s) 77
BstDSI CCRYGG 1 cut(s) 55
BstENI CCTNNNNNAGG 1 cut(s) 284
BstFNI CGCG 5 cut(s) 558, 576, 606, 612, 690
BstH2I RGCGCY 2 cut(s) 63, 487
BstHHI GCGC 9 cut(s) 62, 422, 486, 509, 560, 576, 606, 608, 692
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 1 cut(s) 706
BstMBI GATC 1 cut(s) 95
BstMCI CGRYCG 2 cut(s) 108, 463
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 3 cut(s) 91, 252, 285
BstSLI GKGCMC 2 cut(s) 75, 347
BstUI CGCG 5 cut(s) 558, 576, 606, 612, 690
BstV1I GCAGC 4 cut(s) 140, 168, 486, 532
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 5 cut(s) 107, 257, 566, 587, 617
BtgI CCRYGG 1 cut(s) 55
BtgZI GCGATG 1 cut(s) 502
BtuMI TCGCGA 1 cut(s) 612
Cac8I GCNNGC 8 cut(s) 133, 259, 413, 514, 606, 610, 688, 797
CaiI CAGNNNCTG 2 cut(s) 162, 264
CfoI GCGC 9 cut(s) 62, 422, 486, 509, 560, 576, 606, 608, 692
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 5 cut(s) 168, 184, 289, 472, 622
CseI GACGC 1 cut(s) 226
CspCI CAANNNNNGTGG 2 cut(s) 739, 774
CviAII CATG 2 cut(s) 56, 532
DdeI CTNAG 1 cut(s) 77
DinI GGCGCC 1 cut(s) 61
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
DriI GACNNNNNGTC 1 cut(s) 671
EaeI YGGCCR 2 cut(s) 105, 255
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 2 cut(s) 287, 819
Eam1105I GACNNNNNGTC 1 cut(s) 671
EarI CTCTTC 2 cut(s) 287, 819
EciI GGCGGA 4 cut(s) 153, 206, 233, 415
Ecl136II GAGCTC 1 cut(s) 454
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 3 cut(s) 55, 591, 618
Eco147I AGGCCT 1 cut(s) 617
Eco24I GRGCYC 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 706
Eco47I GGWCC 5 cut(s) 168, 184, 289, 472, 622
Eco52I CGGCCG 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 454
EcoICRI GAGCTC 1 cut(s) 454
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 2 cut(s) 184, 472
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 3 cut(s) 55, 591, 618
EcoT38I GRGCYC 1 cut(s) 456
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
ErhI CCWWGG 3 cut(s) 55, 591, 618
FaeI CATG 2 cut(s) 59, 535
FaiI YATR 3 cut(s) 57, 533, 804
FaqI GGGAC 1 cut(s) 181
FatI CATG 2 cut(s) 55, 531
FauI CCCGC 3 cut(s) 158, 420, 676
FriOI GRGCYC 1 cut(s) 456
GlaI GCGC 9 cut(s) 61, 421, 485, 508, 559, 575, 605, 607, 691
HaeII RGCGCY 2 cut(s) 63, 487
HaeIII GGCC 5 cut(s) 107, 257, 566, 587, 617
HapII CCGG 5 cut(s) 92, 108, 132, 254, 645
HgaI GACGC 1 cut(s) 226
HhaI GCGC 9 cut(s) 62, 422, 486, 509, 560, 576, 606, 608, 692
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 2 cut(s) 59, 535
Hin6I GCGC 9 cut(s) 60, 420, 484, 507, 558, 574, 604, 606, 690
HinP1I GCGC 9 cut(s) 60, 420, 484, 507, 558, 574, 604, 606, 690
HpaII CCGG 5 cut(s) 92, 108, 132, 254, 645
HphI GGTGA 2 cut(s) 833, 847
Hpy188I TCNGA 3 cut(s) 19, 364, 600
Hpy188III TCNNGA 1 cut(s) 611
Hpy99I CGWCG 2 cut(s) 23, 461
HpyAV CCTTC 5 cut(s) 169, 397, 454, 664, 826
HpyCH4V TGCA 4 cut(s) 156, 228, 761, 799
HpyF3I CTNAG 1 cut(s) 77
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 2 cut(s) 59, 535
HspAI GCGC 9 cut(s) 60, 420, 484, 507, 558, 574, 604, 606, 690
KasI GGCGCC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Kzo9I GATC 1 cut(s) 95
LguI GCTCTTC 1 cut(s) 819
LmnI GCTCC 7 cut(s) 34, 125, 341, 639, 653, 713, 844
Lsp1109I GCAGC 4 cut(s) 140, 168, 486, 532
LweI GCATC 2 cut(s) 525, 808
MaeIII GTNAC 1 cut(s) 200
MalI GATC 1 cut(s) 97
MbiI CCGCTC 1 cut(s) 571
MboI GATC 1 cut(s) 95
MboII GAAGA 5 cut(s) 16, 274, 439, 824, 836
MhlI GDGCHC 4 cut(s) 75, 347, 456, 636
MluCI AATT 3 cut(s) 13, 298, 437
Mly113I GGCGCC 1 cut(s) 60
MmeI TCCRAC 4 cut(s) 42, 387, 682, 822
MroNI GCCGGC 1 cut(s) 131
MslI CAYNNNNRTG 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 5 cut(s) 92, 108, 132, 254, 645
MspR9I CCNGG 3 cut(s) 93, 254, 287
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 5 cut(s) 558, 576, 606, 612, 690
NaeI GCCGGC 1 cut(s) 133
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 2 cut(s) 93, 254
NcoI CCATGG 1 cut(s) 55
NdeII GATC 1 cut(s) 95
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 2 cut(s) 59, 535
NlaIV GGNNCC 7 cut(s) 30, 61, 90, 169, 185, 649, 709
NmeAIII GCCGAG 3 cut(s) 83, 586, 592
NruI TCGCGA 1 cut(s) 612
PauI GCGCGC 1 cut(s) 604
PceI AGGCCT 1 cut(s) 617
PciSI GCTCTTC 1 cut(s) 819
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PdiI GCCGGC 1 cut(s) 133
PfoI TCCNGGA 1 cut(s) 285
PluTI GGCGCC 1 cut(s) 63
PpuMI RGGWCCY 2 cut(s) 184, 472
Psp124BI GAGCTC 1 cut(s) 456
Psp5II RGGWCCY 2 cut(s) 184, 472
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 7 cut(s) 30, 61, 90, 169, 185, 649, 709
PspPI GGNCC 5 cut(s) 168, 184, 289, 472, 622
PspPPI RGGWCCY 2 cut(s) 184, 472
PstNI CAGNNNCTG 2 cut(s) 162, 264
PteI GCGCGC 1 cut(s) 604
RruI TCGCGA 1 cut(s) 612
RseI CAYNNNNRTG 1 cut(s) 786
SacI GAGCTC 1 cut(s) 456
SapI GCTCTTC 1 cut(s) 819
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 5 cut(s) 168, 184, 289, 472, 622
ScrFI CCNGG 3 cut(s) 93, 254, 287
SduI GDGCHC 4 cut(s) 75, 347, 456, 636
SetI ASST 9 cut(s) 233, 263, 327, 456, 474, 702, 778, 837, 849
SfaNI GCATC 2 cut(s) 525, 808
SfoI GGCGCC 1 cut(s) 61
SinI GGWCC 5 cut(s) 168, 184, 289, 472, 622
SmiMI CAYNNNNRTG 1 cut(s) 786
Sse9I AATT 3 cut(s) 13, 298, 437
SseBI AGGCCT 1 cut(s) 617
SspDI GGCGCC 1 cut(s) 59
SstI GAGCTC 1 cut(s) 456
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 3 cut(s) 91, 252, 285
StyI CCWWGG 3 cut(s) 55, 591, 618
TaqI TCGA 3 cut(s) 450, 456, 477
TasI AATT 3 cut(s) 13, 298, 437
TseI GCWGC 4 cut(s) 128, 156, 499, 545
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 188
VpaK11BI GGWCC 5 cut(s) 168, 184, 289, 472, 622
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.