Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0015862

Physical Location & Seq

Genomic Coordinates Reverse (-)

3287868 .. 3290992

Gene Structure 3125 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026480

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGCACCTCCTCGAGGCCGTGGTGGGTTCAGGGGTGGAAGGAGTGATGGAGGGAGAGGCCGAGGTGGAGGCCGTGGATTCGGTGACAGAAGTAACAGTGGTTTTAAGCCCAGTGGAGGCCGAGGTGACCGTGGTGGTCGGGGCCGTGGTGACCGCGGCCGTGGAAGAGGTGGCCGTGGGGGTAGAGGAGGAATGAAGGGCGGAACTAAGGTTGTGGTTGAGCCCCATAGACATGCTGGGGTTTTCCTCGCTAAGGGTAAAGAAGATGCTCTTGTGACCAAGAACATGGTCCCTGGAGAAGCTGTCTACAATGAGAAGAGGATTTCCGTTCAGCAAGAGGATGGAACAAAAGTTGAGTACAGGATTTGGAACCCTTTCCGTTCCAAGTTGGCAGCTGCTATTCTTGGTGGTGTTGATGAGATCTGGATTAAACCTGGTGCCAAGGTTCTCTACCTGGGGGCTGCTTCTGGTACCACAGTCTCTCATGTCTCCGACATTGTTGGCCCTACTGGATGTGTCTATGCAGTGGAGTTTTCTCATAGAAGTGGCAGAGACTTGGTAAACATGGCTAAGAAGAGAACTAATGTCATACCAATTATTGAAGATGCTAGGCATCCAGCAAAATACCGTATGCTTGTTGGCATGGTTGACGTGATATTTTCTGATGTTGCACAACCCGATCAGGCCAGGATTCTAGCTTTGAATGCATCATATTTTCTTAAAGCCGGAGGGCACTTCGTCATCTCTATCAAGGCTAATTGTATCGACTGCACTGTTCCTGCTGAGGCAGTGTTCCAATCTGAGGTGAAGAAGCTGCAGGCAGAACAGTTTAAGCCTTTTGAACAAGTGACTCTTGAACCTTTTGAGCGTGATCATGCTTGTGTGATTGGTGGTTACCGCATGCCAAAGAAGTCAAAAGGCTACTGCCTAGAACGTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000494 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005732 GO:0006139 GO:0006325 GO:0006364 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008213 GO:0008276 GO:0008649 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0015030 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0016074 GO:0016569 GO:0016570 GO:0016571 GO:0016592 GO:0016604 GO:0016740 GO:0016741 GO:0016787 GO:0016788 GO:0018364 GO:0019538 GO:0022613 GO:0030515 GO:0030684 GO:0031123 GO:0031126 GO:0031167 GO:0031428 GO:0031974 GO:0031981 GO:0032040 GO:0032259 GO:0032991 GO:0033967 GO:0034470 GO:0034641 GO:0034660 GO:0034963 GO:0036009 GO:0036211 GO:0042054 GO:0042254 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0046483 GO:0051276 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0140096 GO:0140098 GO:0140102 GO:1901360 GO:1901363 GO:1901564 GO:1990258 GO:1990259 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Fibrillarin

Protein Analysis

314

Amino Acids

33.74

Weight (kDa)

10.01

Isoelectric Point (pI)

30.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fibrillarin	PF01269	73 - 300	2.8e-109	Fibrillarin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52470
fragaria_vesca	FvH4_7g30020
rosa_chinensis	RchiOBHm_Chr1g0377981
rosa_laevigata	RLG00000026480
rosa_multiflora	Rmu_sc0002068.1_g000004
rosa_roxburghii	Rroxscaffold_156G00440430 Rroxscaffold_4G00280860
rosa_rugosa	Rorug01G0406000 Rorug01G0406000 Rorug02G0371900
rosa_samantha	Rh1AG425300 Rh1BG384000 Rh1CG397800 Rh1DG415300
rosa_wichuraiana	Rw1G037300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	12
Acc65I	GGTACC	1 cut(s)	470
AccB1I	GGYRCC	3 cut(s)	4, 437, 470
AccI	GTMKAC	1 cut(s)	306
AccII	CGCG	1 cut(s)	156
AciI	CCGC	4 cut(s)	154, 156, 201, 898
AcoI	YGGCCR	2 cut(s)	157, 172
AfaI	GTAC	2 cut(s)	359, 472
AfiI	CCNNNNNNNGG	4 cut(s)	14, 116, 253, 802
AgsI	TTSAA	4 cut(s)	602, 703, 842, 857
AjiI	CACGTC	1 cut(s)	652
AjnI	CCWGG	4 cut(s)	292, 433, 453, 686
AluBI	AGCT	4 cut(s)	302, 395, 698, 814
AluI	AGCT	4 cut(s)	302, 395, 698, 814
Alw26I	GTCTC	3 cut(s)	484, 493, 546
Ama87I	CYCGRG	1 cut(s)	12
AoxI	GGCC	9 cut(s)	16, 58, 70, 118, 142, 157, 172, 502, 684
ApeKI	GCWGC	4 cut(s)	392, 395, 461, 814
Asp700I	GAANNNNTTC	1 cut(s)	374
Asp718I	GGTACC	1 cut(s)	470
AspS9I	GGNCC	3 cut(s)	142, 289, 503
AsuHPI	GGTGA	4 cut(s)	95, 137, 161, 817
AvaI	CYCGRG	1 cut(s)	12
AvaII	GGWCC	1 cut(s)	289
BaeGI	GKGCMC	1 cut(s)	735
BaeI	ACNNNNGTAYC	2 cut(s)	462, 495
BanI	GGYRCC	3 cut(s)	4, 437, 470
BanII	GRGCYC	1 cut(s)	225
BbvCI	CCTCAGC	1 cut(s)	783
BbvI	GCAGC	4 cut(s)	382, 404, 448, 801
BccI	CCATC	2 cut(s)	41, 335
BceAI	ACGGC	5 cut(s)	3, 57, 129, 144, 159
BciT130I	CCWGG	4 cut(s)	294, 435, 455, 688
BclI	TGATCA	1 cut(s)	871
BcoDI	GTCTC	3 cut(s)	484, 493, 546
BfaI	CTAG	3 cut(s)	609, 695, 929
BfmI	CTRYAG	1 cut(s)	815
BglII	AGATCT	1 cut(s)	420
BisI	GCNGC	5 cut(s)	157, 393, 396, 462, 815
BlsI	GCNGC	5 cut(s)	158, 394, 397, 463, 816
Bme1390I	CCNGG	4 cut(s)	294, 435, 455, 688
Bme18I	GGWCC	1 cut(s)	289
BmeT110I	CYCGRG	1 cut(s)	12
BmgBI	CACGTC	1 cut(s)	652
BmgT120I	GGNCC	3 cut(s)	142, 289, 503
BmiI	GGNNCC	6 cut(s)	6, 143, 291, 371, 439, 472
BmrFI	CCNGG	4 cut(s)	294, 435, 455, 688
BmrI	ACTGGG	1 cut(s)	105
BmsI	GCATC	4 cut(s)	256, 595, 622, 716
BmuI	ACTGGG	1 cut(s)	105
BpmI	CTGGAG	1 cut(s)	315
Bpu10I	CCTNAGC	2 cut(s)	252, 783
BsaXI	ACNNNNNCTCC	2 cut(s)	288, 318
Bsc4I	CCNNNNNNNGG	4 cut(s)	14, 116, 253, 802
Bse1I	ACTGG	2 cut(s)	111, 514
BseBI	CCWGG	4 cut(s)	294, 435, 455, 688
BseGI	GGATG	3 cut(s)	346, 518, 613
BseLI	CCNNNNNNNGG	4 cut(s)	14, 116, 253, 802
BseMII	CTCAG	2 cut(s)	774, 792
BseNI	ACTGG	2 cut(s)	111, 514
BseRI	GAGGAG	1 cut(s)	201
BseSI	GKGCMC	1 cut(s)	735
BseX3I	CGGCCG	1 cut(s)	157
BseXI	GCAGC	4 cut(s)	382, 404, 448, 801
BseYI	CCCAGC	1 cut(s)	236
BsgI	GTGCAG	1 cut(s)	754
Bsh1236I	CGCG	1 cut(s)	156
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	9 cut(s)	18, 60, 72, 120, 144, 159, 174, 504, 686
BshNI	GGYRCC	3 cut(s)	4, 437, 470
BsiEI	CGRYCG	1 cut(s)	160
BsiHKCI	CYCGRG	1 cut(s)	12
BsiSI	CCGG	1 cut(s)	726
BslFI	GGGAC	1 cut(s)	275
BslI	CCNNNNNNNGG	4 cut(s)	14, 116, 253, 802
BsmAI	GTCTC	3 cut(s)	484, 493, 546
BsmFI	GGGAC	1 cut(s)	275
BsmI	GAATGC	1 cut(s)	709
BsnI	GGCC	9 cut(s)	18, 60, 72, 120, 144, 159, 174, 504, 686
BsoBI	CYCGRG	1 cut(s)	12
Bsp1286I	GDGCHC	2 cut(s)	225, 735
Bsp143I	GATC	3 cut(s)	420, 679, 871
BspACI	CCGC	4 cut(s)	154, 156, 201, 898
BspANI	GGCC	9 cut(s)	18, 60, 72, 120, 144, 159, 174, 504, 686
BspCNI	CTCAG	2 cut(s)	775, 793
BspFNI	CGCG	1 cut(s)	156
BspLI	GGNNCC	6 cut(s)	6, 143, 291, 371, 439, 472
BspMAI	CTGCAG	1 cut(s)	819
BspT107I	GGYRCC	3 cut(s)	4, 437, 470
BsrI	ACTGG	2 cut(s)	111, 514
BssMI	GATC	3 cut(s)	420, 679, 871
BssT1I	CCWWGG	1 cut(s)	441
Bst2UI	CCWGG	4 cut(s)	294, 435, 455, 688
Bst4CI	ACNGT	6 cut(s)	98, 131, 478, 629, 775, 828
Bst6I	CTCTTC	3 cut(s)	160, 311, 569
BstC8I	GCNNGC	2 cut(s)	819, 902
BstDEI	CTNAG	5 cut(s)	207, 252, 570, 783, 801
BstDSI	CCRYGG	7 cut(s)	19, 73, 130, 145, 154, 160, 175
BstEII	GGTNACC	3 cut(s)	125, 149, 893
BstENI	CCTNNNNNAGG	2 cut(s)	12, 251
BstF5I	GGATG	3 cut(s)	346, 518, 613
BstFNI	CGCG	1 cut(s)	156
BstKTI	GATC	3 cut(s)	423, 682, 874
BstMAI	GTCTC	3 cut(s)	484, 493, 546
BstMBI	GATC	3 cut(s)	420, 679, 871
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	1 cut(s)	704
BstNI	CCWGG	4 cut(s)	294, 435, 455, 688
BstNSI	RCATGY	2 cut(s)	236, 904
BstPI	GGTNACC	3 cut(s)	125, 149, 893
BstSCI	CCNGG	4 cut(s)	292, 433, 453, 686
BstSFI	CTRYAG	1 cut(s)	815
BstSLI	GKGCMC	1 cut(s)	735
BstUI	CGCG	1 cut(s)	156
BstV1I	GCAGC	4 cut(s)	382, 404, 448, 801
BstX2I	RGATCY	1 cut(s)	420
BstXI	CCANNNNNNTGG	1 cut(s)	286
BstYI	RGATCY	1 cut(s)	420
BstZI	CGGCCG	1 cut(s)	157
BsuRI	GGCC	9 cut(s)	18, 60, 72, 120, 144, 159, 174, 504, 686
BtgI	CCRYGG	7 cut(s)	19, 73, 130, 145, 154, 160, 175
BtrI	CACGTC	1 cut(s)	652
BtsCI	GGATG	3 cut(s)	346, 518, 613
BtsI	GCAGTG	2 cut(s)	531, 795
BtsIMutI	CAGTG	5 cut(s)	103, 118, 531, 771, 795
Cac8I	GCNNGC	2 cut(s)	819, 902
Cfr13I	GGNCC	3 cut(s)	142, 289, 503
Cfr42I	CCGCGG	1 cut(s)	157
CsiI	ACCWGGT	1 cut(s)	433
Csp6I	GTAC	2 cut(s)	358, 471
CviAII	CATG	7 cut(s)	233, 286, 485, 565, 643, 875, 901
CviQI	GTAC	2 cut(s)	358, 471
DdeI	CTNAG	5 cut(s)	207, 252, 570, 783, 801
DpnI	GATC	3 cut(s)	422, 681, 873
DpnII	GATC	3 cut(s)	420, 679, 871
EaeI	YGGCCR	2 cut(s)	157, 172
EagI	CGGCCG	1 cut(s)	157
Eam1104I	CTCTTC	3 cut(s)	160, 311, 569
EarI	CTCTTC	3 cut(s)	160, 311, 569
EciI	GGCGGA	1 cut(s)	216
EclXI	CGGCCG	1 cut(s)	157
Eco130I	CCWWGG	1 cut(s)	441
Eco24I	GRGCYC	1 cut(s)	225
Eco47I	GGWCC	1 cut(s)	289
Eco52I	CGGCCG	1 cut(s)	157
Eco88I	CYCGRG	1 cut(s)	12
Eco91I	GGTNACC	3 cut(s)	125, 149, 893
EcoNI	CCTNNNNNAGG	2 cut(s)	12, 251
EcoO65I	GGTNACC	3 cut(s)	125, 149, 893
EcoRII	CCWGG	4 cut(s)	292, 433, 453, 686
EcoT14I	CCWWGG	1 cut(s)	441
EcoT22I	ATGCAT	1 cut(s)	709
EcoT38I	GRGCYC	1 cut(s)	225
ErhI	CCWWGG	1 cut(s)	441
FaeI	CATG	7 cut(s)	236, 289, 488, 568, 646, 878, 904
FalI	AAGNNNNNCTT	4 cut(s)	255, 287, 837, 869
FaqI	GGGAC	1 cut(s)	275
FatI	CATG	7 cut(s)	232, 285, 484, 564, 642, 874, 900
FbaI	TGATCA	1 cut(s)	871
FblI	GTMKAC	1 cut(s)	306
Fnu4HI	GCNGC	5 cut(s)	157, 393, 396, 462, 815
FokI	GGATG	3 cut(s)	353, 525, 600
FriOI	GRGCYC	1 cut(s)	225
Fsp4HI	GCNGC	5 cut(s)	157, 393, 396, 462, 815
FspBI	CTAG	3 cut(s)	609, 695, 929
GluI	GCNGC	5 cut(s)	157, 393, 396, 462, 815
GsaI	CCCAGC	1 cut(s)	240
GsuI	CTGGAG	1 cut(s)	315
HaeIII	GGCC	9 cut(s)	18, 60, 72, 120, 144, 159, 174, 504, 686
HapII	CCGG	1 cut(s)	726
Hin1II	CATG	7 cut(s)	236, 289, 488, 568, 646, 878, 904
HincII	GTYRAC	1 cut(s)	649
HindII	GTYRAC	1 cut(s)	649
HinfI	GANTC	3 cut(s)	78, 691, 850
HpaII	CCGG	1 cut(s)	726
HphI	GGTGA	4 cut(s)	95, 137, 161, 817
Hpy166II	GTNNAC	3 cut(s)	307, 562, 649
Hpy188I	TCNGA	3 cut(s)	493, 664, 802
Hpy188III	TCNNGA	2 cut(s)	424, 854
Hpy8I	GTNNAC	3 cut(s)	307, 562, 649
HpyAV	CCTTC	2 cut(s)	33, 190
HpyCH4III	ACNGT	6 cut(s)	98, 131, 478, 629, 775, 828
HpyCH4IV	ACGT	2 cut(s)	651, 934
HpyCH4V	TGCA	5 cut(s)	524, 671, 707, 771, 817
HpyF10VI	GCNNNNNNNGC	1 cut(s)	704
HpyF3I	CTNAG	5 cut(s)	207, 252, 570, 783, 801
HpySE526I	ACGT	2 cut(s)	651, 934
Hsp92II	CATG	7 cut(s)	236, 289, 488, 568, 646, 878, 904
KpnI	GGTACC	1 cut(s)	474
Ksp22I	TGATCA	1 cut(s)	871
KspI	CCGCGG	1 cut(s)	157
Kzo9I	GATC	3 cut(s)	420, 679, 871
Lsp1109I	GCAGC	4 cut(s)	382, 404, 448, 801
LweI	GCATC	4 cut(s)	256, 595, 622, 716
MabI	ACCWGGT	1 cut(s)	433
MaeI	CTAG	3 cut(s)	609, 695, 929
MaeII	ACGT	2 cut(s)	651, 934
MaeIII	GTNAC	7 cut(s)	83, 92, 125, 149, 274, 847, 893
MalI	GATC	3 cut(s)	422, 681, 873
MboI	GATC	3 cut(s)	420, 679, 871
MboII	GAAGA	6 cut(s)	177, 275, 328, 586, 614, 820
MflI	RGATCY	1 cut(s)	420
MhlI	GDGCHC	2 cut(s)	225, 735
MluCI	AATT	2 cut(s)	594, 757
MlyI	GAGTC	1 cut(s)	844
MmeI	TCCRAC	1 cut(s)	516
Mph1103I	ATGCAT	1 cut(s)	709
MroXI	GAANNNNTTC	1 cut(s)	374
MseI	TTAA	4 cut(s)	105, 429, 720, 831
MslI	CAYNNNNRTG	3 cut(s)	231, 543, 879
MspA1I	CMGCKG	2 cut(s)	156, 395
MspI	CCGG	1 cut(s)	726
MspR9I	CCNGG	4 cut(s)	294, 435, 455, 688
Mva1269I	GAATGC	1 cut(s)	709
MvaI	CCWGG	4 cut(s)	294, 435, 455, 688
MvnI	CGCG	1 cut(s)	156
MwoI	GCNNNNNNNGC	1 cut(s)	704
NdeII	GATC	3 cut(s)	420, 679, 871
NlaIII	CATG	7 cut(s)	236, 289, 488, 568, 646, 878, 904
NlaIV	GGNNCC	6 cut(s)	6, 143, 291, 371, 439, 472
NmeAIII	GCCGAG	2 cut(s)	86, 146
NmuCI	GTSAC	5 cut(s)	83, 125, 149, 274, 847
NsiI	ATGCAT	1 cut(s)	709
NspI	RCATGY	2 cut(s)	236, 904
PaeI	GCATGC	1 cut(s)	904
PaeR7I	CTCGAG	1 cut(s)	12
PctI	GAATGC	1 cut(s)	709
PdmI	GAANNNNTTC	1 cut(s)	374
PfeI	GAWTC	2 cut(s)	78, 691
PkrI	GCNGC	5 cut(s)	158, 394, 397, 463, 816
PleI	GAGTC	1 cut(s)	844
PpsI	GAGTC	1 cut(s)	844
Psp6I	CCWGG	4 cut(s)	292, 433, 453, 686
PspEI	GGTNACC	3 cut(s)	125, 149, 893
PspFI	CCCAGC	1 cut(s)	236
PspGI	CCWGG	4 cut(s)	292, 433, 453, 686
PspN4I	GGNNCC	6 cut(s)	6, 143, 291, 371, 439, 472
PspPI	GGNCC	3 cut(s)	142, 289, 503
PspXI	VCTCGAGB	1 cut(s)	12
PstI	CTGCAG	1 cut(s)	819
PsuI	RGATCY	1 cut(s)	420
PvuII	CAGCTG	1 cut(s)	395
RsaI	GTAC	2 cut(s)	359, 472
RsaNI	GTAC	2 cut(s)	358, 471
RseI	CAYNNNNRTG	3 cut(s)	231, 543, 879
SacII	CCGCGG	1 cut(s)	157
SaqAI	TTAA	4 cut(s)	105, 429, 720, 831
SatI	GCNGC	5 cut(s)	157, 393, 396, 462, 815
Sau3AI	GATC	3 cut(s)	420, 679, 871
Sau96I	GGNCC	3 cut(s)	142, 289, 503
SchI	GAGTC	1 cut(s)	844
ScrFI	CCNGG	4 cut(s)	294, 435, 455, 688
SduI	GDGCHC	2 cut(s)	225, 735
SexAI	ACCWGGT	1 cut(s)	433
SfaNI	GCATC	4 cut(s)	256, 595, 622, 716
SfcI	CTRYAG	1 cut(s)	815
Sfr274I	CTCGAG	1 cut(s)	12
Sfr303I	CCGCGG	1 cut(s)	157
SgrBI	CCGCGG	1 cut(s)	157
SinI	GGWCC	1 cut(s)	289
SlaI	CTCGAG	1 cut(s)	12
SmiMI	CAYNNNNRTG	3 cut(s)	231, 543, 879
SmlI	CTYRAG	1 cut(s)	12
SmoI	CTYRAG	1 cut(s)	12
SphI	GCATGC	1 cut(s)	904
Sse9I	AATT	2 cut(s)	594, 757
SsiI	CCGC	4 cut(s)	154, 156, 201, 898
SspMI	CTAG	3 cut(s)	609, 695, 929
StyD4I	CCNGG	4 cut(s)	292, 433, 453, 686
StyI	CCWWGG	1 cut(s)	441
TaaI	ACNGT	6 cut(s)	98, 131, 478, 629, 775, 828
TaiI	ACGT	2 cut(s)	654, 937
TaqI	TCGA	2 cut(s)	13, 765
TasI	AATT	2 cut(s)	594, 757
TatI	WGTACW	1 cut(s)	357
TauI	GCSGC	1 cut(s)	159
TfiI	GAWTC	2 cut(s)	78, 691
Tru1I	TTAA	4 cut(s)	105, 429, 720, 831
Tru9I	TTAA	4 cut(s)	105, 429, 720, 831
TscAI	CASTG	5 cut(s)	103, 118, 531, 778, 795
TseFI	GTSAC	5 cut(s)	83, 125, 149, 274, 847
TseI	GCWGC	4 cut(s)	392, 395, 461, 814
Tsp45I	GTSAC	5 cut(s)	83, 125, 149, 274, 847
TspDTI	ATGAA	1 cut(s)	209
TspGWI	ACGGA	2 cut(s)	316, 368
TspRI	CASTG	5 cut(s)	103, 118, 531, 778, 795
VpaK11BI	GGWCC	1 cut(s)	289
XagI	CCTNNNNNAGG	2 cut(s)	12, 251
XceI	RCATGY	2 cut(s)	236, 904
XcmI	CCANNNNNNNNNTGG	1 cut(s)	233
XhoI	CTCGAG	1 cut(s)	12
XmiI	GTMKAC	1 cut(s)	306
XmnI	GAANNNNTTC	1 cut(s)	374
XspI	CTAG	3 cut(s)	609, 695, 929
Zsp2I	ATGCAT	1 cut(s)	709

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.