RLG00000027868

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017387
Physical Location & Seq
Forward (+)
16256833 .. 16258053
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027868

Sequence Viewer

Length: 732 bp
ATGGGAGCTATGAGGATGGAATGGTCTGCAGCATCTGCAACAAAAGCTTACTTTGACACCATCAAACTGTGCAATGATCGCAGAAGACAATGTGACTCAGTGAAGTTAACACAAGAGCCAGGGAGCAATGAATTCGTGTCAGCCTTGGCAGCAGGCATGAAAGCCAAGCTCATAGTGGAGGTGACATCAAGTGCTTCCCCATCAACAATTGCCTTGGCAGCTGCTGCAAAACACACCGGAGGCAAATTGGTTTGCATCCTTCCGGAGCCAGATCTAGCTGAATCGAAGAAAGTGATCAATGACTCAGGCCTAAAAGACTTGGTAGAATTCAAGACAGGTGACCCTTTTGAGCTGTTGAGGAATTACGAGAACATTGATTTCTCTCTCGTTGATTGCAAGAATGATGAGTACACAGGGTTGCTGAAGTTGCTGGATATTAATCCGAGAAAATCAGTGGTTGTGGCTACTAACTTGGTTGGGGAAAGAAAAGGATTCGAAGGACATGTGAAGGGAGTGAAGGAGGAGAGGGTTGTCGTGAGGTCTATGAAGCATCCCATTGGAAAAGGTATGGAGGTCACCATGATCGGAAAGGAAGATTGGGGCGGTGGAAGAATTTCGAATGAAGCAGAAAAAAGGGGTGGTTTCAAGAAGAGGAATGGTAATAAGAGCAAATGGCTTGTCAAGGTTGATGAGAAGAGTGGTGAGGAACATATCTTTAGGATTCGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

244

Amino Acids

26.74

Weight (kDa)

9.14

Isoelectric Point (pI)

25.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 4 - 240 6.9e-33 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017387)

Species Orthologous Gene IDs
malus_domestica MD01G1080700.v1.1 MD07G1149600.v1.1 MD07G1149700.v1.1
prunus_persica Prupe.2G188300_v2.0.a1
pyrus_communis pycom07g14830
rosa_chinensis RchiOBHm_Chr1g0359471
rosa_laevigata RLG00000027868
rosa_multiflora Rmu_sc0002556.1_g000003
rosa_roxburghii Rroxscaffold_4G00296980
rosa_samantha Rh1AG284800 Rh1BG250800 Rh1DG279900
rosa_wichuraiana Rw1G025320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 262
AciI CCGC 1 cut(s) 603
AcsI RAATTY 3 cut(s) 131, 326, 612
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 2 cut(s) 331, 646
AjnI CCWGG 1 cut(s) 118
AjuI GAANNNNNNNTTGG 2 cut(s) 580, 612
AluBI AGCT 6 cut(s) 8, 47, 169, 221, 278, 352
AluI AGCT 6 cut(s) 8, 47, 169, 221, 278, 352
AlwNI CAGNNNCTG 2 cut(s) 35, 224
Aor13HI TCCGGA 1 cut(s) 262
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 5 cut(s) 29, 149, 218, 221, 224
ApoI RAATTY 3 cut(s) 131, 326, 612
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
AseI ATTAAT 1 cut(s) 438
Asp700I GAANNNNTTC 1 cut(s) 613
AsuHPI GGTGA 4 cut(s) 193, 350, 568, 713
AsuII TTCGAA 2 cut(s) 495, 617
BbsI GAAGAC 1 cut(s) 91
BbvI GCAGC 5 cut(s) 41, 161, 208, 211, 230
BccI CCATC 3 cut(s) 10, 68, 208
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 120
BclI TGATCA 1 cut(s) 294
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 27
BglII AGATCT 1 cut(s) 271
BisI GCNGC 5 cut(s) 30, 150, 219, 222, 225
BlsI GCNGC 5 cut(s) 31, 151, 220, 223, 226
Bme1390I CCNGG 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 3 cut(s) 41, 264, 559
BpiI GAAGAC 1 cut(s) 91
Bpu14I TTCGAA 2 cut(s) 495, 617
BsaBI GATNNNNATC 1 cut(s) 438
BsaJI CCNNGG 3 cut(s) 119, 144, 213
BsaWI WCCGGW 2 cut(s) 236, 262
Bse3DI GCAATG 2 cut(s) 79, 133
Bse8I GATNNNNATC 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 262
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 3 cut(s) 119, 144, 213
BseGI GGATG 3 cut(s) 21, 255, 550
BseJI GATNNNNATC 1 cut(s) 438
BseMI GCAATG 2 cut(s) 79, 133
BseMII CTCAG 2 cut(s) 111, 318
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 5 cut(s) 41, 161, 208, 211, 230
BshFI GGCC 1 cut(s) 309
BsiSI CCGG 2 cut(s) 237, 263
BsnI GGCC 1 cut(s) 309
Bsp119I TTCGAA 2 cut(s) 495, 617
Bsp13I TCCGGA 1 cut(s) 262
Bsp143I GATC 4 cut(s) 76, 271, 294, 582
BspACI CCGC 1 cut(s) 603
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 2 cut(s) 110, 317
BspEI TCCGGA 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 31
BspT104I TTCGAA 2 cut(s) 495, 617
BsrDI GCAATG 2 cut(s) 79, 133
BssECI CCNNGG 3 cut(s) 119, 144, 213
BssMI GATC 4 cut(s) 76, 271, 294, 582
BssT1I CCWWGG 2 cut(s) 144, 213
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 2 cut(s) 644, 689
BstAPI GCANNNNNTGC 2 cut(s) 35, 224
BstBI TTCGAA 2 cut(s) 495, 617
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 2 cut(s) 97, 304
BstEII GGTNACC 2 cut(s) 338, 574
BstF5I GGATG 3 cut(s) 21, 255, 550
BstKTI GATC 4 cut(s) 79, 274, 297, 585
BstMBI GATC 4 cut(s) 76, 271, 294, 582
BstMWI GCNNNNNNNGC 7 cut(s) 35, 44, 78, 149, 218, 224, 427
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 1 cut(s) 506
BstPI GGTNACC 2 cut(s) 338, 574
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 5 cut(s) 41, 161, 208, 211, 230
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 1 cut(s) 309
BtsCI GGATG 3 cut(s) 21, 255, 550
BtsIMutI CAGTG 2 cut(s) 105, 459
Cac8I GCNNGC 1 cut(s) 154
CaiI CAGNNNCTG 2 cut(s) 35, 224
Csp6I GTAC 1 cut(s) 409
CviAII CATG 3 cut(s) 157, 503, 580
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 2 cut(s) 97, 304
DpnI GATC 4 cut(s) 78, 273, 296, 584
DpnII GATC 4 cut(s) 76, 271, 294, 582
Eam1104I CTCTTC 2 cut(s) 644, 689
EarI CTCTTC 2 cut(s) 644, 689
Eco130I CCWWGG 2 cut(s) 144, 213
Eco147I AGGCCT 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 443
Eco91I GGTNACC 2 cut(s) 338, 574
EcoO65I GGTNACC 2 cut(s) 338, 574
EcoRI GAATTC 2 cut(s) 131, 326
EcoRII CCWGG 1 cut(s) 118
EcoT14I CCWWGG 2 cut(s) 144, 213
ErhI CCWWGG 2 cut(s) 144, 213
FaeI CATG 3 cut(s) 160, 506, 583
FaiI YATR 8 cut(s) 11, 158, 173, 504, 545, 569, 581, 711
FatI CATG 3 cut(s) 156, 502, 579
FbaI TGATCA 1 cut(s) 294
Fnu4HI GCNGC 5 cut(s) 30, 150, 219, 222, 225
FokI GGATG 3 cut(s) 28, 242, 537
Fsp4HI GCNGC 5 cut(s) 30, 150, 219, 222, 225
FspBI CTAG 1 cut(s) 275
GluI GCNGC 5 cut(s) 30, 150, 219, 222, 225
HaeIII GGCC 1 cut(s) 309
HapII CCGG 2 cut(s) 237, 263
Hin1II CATG 3 cut(s) 160, 506, 583
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 1 cut(s) 45
HinfI GANTC 5 cut(s) 95, 281, 302, 492, 721
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 2 cut(s) 237, 263
HphI GGTGA 4 cut(s) 193, 350, 568, 713
Hpy166II GTNNAC 2 cut(s) 108, 411
Hpy188I TCNGA 2 cut(s) 444, 587
Hpy188III TCNNGA 4 cut(s) 263, 331, 535, 646
Hpy8I GTNNAC 2 cut(s) 108, 411
HpyAV CCTTC 4 cut(s) 269, 491, 502, 511
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4V TGCA 6 cut(s) 29, 38, 72, 227, 255, 396
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 44, 78, 149, 218, 224, 427
HpyF3I CTNAG 2 cut(s) 97, 304
Hsp92II CATG 3 cut(s) 160, 506, 583
Kpn2I TCCGGA 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 294
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 4 cut(s) 76, 271, 294, 582
LmnI GCTCC 3 cut(s) 5, 123, 265
Lsp1109I GCAGC 5 cut(s) 41, 161, 208, 211, 230
LweI GCATC 3 cut(s) 41, 264, 559
MaeI CTAG 1 cut(s) 275
MaeIII GTNAC 4 cut(s) 92, 181, 338, 574
MalI GATC 4 cut(s) 78, 273, 296, 584
MboI GATC 4 cut(s) 76, 271, 294, 582
MboII GAAGA 6 cut(s) 96, 298, 605, 621, 661, 706
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 1 cut(s) 271
MluCI AATT 6 cut(s) 131, 207, 245, 326, 361, 612
MlyI GAGTC 2 cut(s) 89, 296
MroI TCCGGA 1 cut(s) 262
MroXI GAANNNNTTC 1 cut(s) 613
MseI TTAA 2 cut(s) 107, 438
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 2 cut(s) 237, 263
MspR9I CCNGG 1 cut(s) 120
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 7 cut(s) 35, 44, 78, 149, 218, 224, 427
NdeII GATC 4 cut(s) 76, 271, 294, 582
NlaIII CATG 3 cut(s) 160, 506, 583
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 4 cut(s) 92, 181, 338, 574
NspI RCATGY 1 cut(s) 506
NspV TTCGAA 2 cut(s) 495, 617
PceI AGGCCT 1 cut(s) 309
PciI ACATGT 1 cut(s) 502
PdmI GAANNNNTTC 1 cut(s) 613
PfeI GAWTC 3 cut(s) 281, 492, 721
PkrI GCNGC 5 cut(s) 31, 151, 220, 223, 226
PleI GAGTC 2 cut(s) 89, 296
PpsI GAGTC 2 cut(s) 89, 296
PscI ACATGT 1 cut(s) 502
PshBI ATTAAT 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 118
PspEI GGTNACC 2 cut(s) 338, 574
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 267
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 2 cut(s) 35, 224
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
SaqAI TTAA 2 cut(s) 107, 438
SatI GCNGC 5 cut(s) 30, 150, 219, 222, 225
Sau3AI GATC 4 cut(s) 76, 271, 294, 582
SchI GAGTC 2 cut(s) 89, 296
ScrFI CCNGG 1 cut(s) 120
SfaNI GCATC 3 cut(s) 41, 264, 559
SfcI CTRYAG 1 cut(s) 27
SfuI TTCGAA 2 cut(s) 495, 617
Sse9I AATT 6 cut(s) 131, 207, 245, 326, 361, 612
SseBI AGGCCT 1 cut(s) 309
SsiI CCGC 1 cut(s) 603
SspMI CTAG 1 cut(s) 275
StuI AGGCCT 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 2 cut(s) 144, 213
TaaI ACNGT 1 cut(s) 69
TaqI TCGA 3 cut(s) 284, 495, 617
TasI AATT 6 cut(s) 131, 207, 245, 326, 361, 612
TatI WGTACW 1 cut(s) 408
TfiI GAWTC 3 cut(s) 281, 492, 721
Tru1I TTAA 2 cut(s) 107, 438
Tru9I TTAA 2 cut(s) 107, 438
TscAI CASTG 2 cut(s) 105, 459
TseFI GTSAC 4 cut(s) 92, 181, 338, 574
TseI GCWGC 5 cut(s) 29, 149, 218, 221, 224
Tsp45I GTSAC 4 cut(s) 92, 181, 338, 574
TspDTI ATGAA 4 cut(s) 144, 173, 560, 636
TspRI CASTG 2 cut(s) 105, 459
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 3 cut(s) 131, 326, 612
XceI RCATGY 1 cut(s) 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmnI GAANNNNTTC 1 cut(s) 613
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.