Rroxscaffold_4G00296980

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017387
Physical Location & Seq
Forward (+)
16981780 .. 16983890
2111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296980.1

Sequence Viewer

Length: 738 bp
ATGGTCATGGGGGCTATGAGGATGGAATGGTCTGCAGCATCTGCAACAAAAGCTTACTTTGACACCATCAAACTGTGCAATGATCGCAGAAGACAATGCGACTCAGTGAAGTTAACACAAGAGCCAGGGAGCAATGAATTCGTATCAGCCTTGGCAGCAGGCATGAAAGCCAAACTCATAGTGGAGGTGACATCAAGTGCTTCCCCATCAACAATTGCCTTAGCAGCTGCCGCAAAACACACCGGAGGCAAATTGGTTTGCATCCTTCCGGAGCCAGATCTAGCTGAATCGAAGAAAGTGATCAAAGACTCAGGCCTAAAAGACTTGGTAGAATTCAAGACAGGTGACCCTTTTGAGCTACTGACGAATTACGAGAACATTGATTTCTCTCTGGTTGATTGCAAGAATGATGAGTACACAGGGTTGCTGAAGTTGCTGGATGTTAATCCGAGAAAATCAGTGGTAGTGGCTACTAACTTGGTTGGGGAAAGAAAAGGATTCGAAAGACATGTGAAGGGAGTGAAGGAGAAGAGGGCTGTCGTGAGGTCTATGAAGCATCCCATTGGAAAAGGTATGGAGGTCACCATGATCGGAAAGGAAGATTGGGGCGGTGGAAGAGTTTCGAATGAAGCAGAAAAAAGTGGTGGTTTCAAGAAGAGGAATGGTAATAAGAGCAAATGGCTTGTCAAGGTTGATGAGAAGAGTGGTGAGGAACATATCTTTAGGATTCGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

245

Amino Acids

26.9

Weight (kDa)

9.3

Isoelectric Point (pI)

22.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 6 - 242 5.3e-33 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017387)

Species Orthologous Gene IDs
malus_domestica MD01G1080700.v1.1 MD07G1149600.v1.1 MD07G1149700.v1.1
prunus_persica Prupe.2G188300_v2.0.a1
pyrus_communis pycom07g14830
rosa_chinensis RchiOBHm_Chr1g0359471
rosa_laevigata RLG00000027868
rosa_multiflora Rmu_sc0002556.1_g000003
rosa_roxburghii Rroxscaffold_4G00296980
rosa_samantha Rh1AG284800 Rh1BG250800 Rh1DG279900
rosa_wichuraiana Rw1G025320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 268
AciI CCGC 2 cut(s) 231, 609
AcsI RAATTY 2 cut(s) 137, 332
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 1 cut(s) 416
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 2 cut(s) 337, 652
AjnI CCWGG 1 cut(s) 124
AjuI GAANNNNNNNTTGG 2 cut(s) 586, 618
AluBI AGCT 4 cut(s) 53, 227, 284, 358
AluI AGCT 4 cut(s) 53, 227, 284, 358
AlwNI CAGNNNCTG 1 cut(s) 41
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 4 cut(s) 35, 155, 224, 227
ApoI RAATTY 2 cut(s) 137, 332
ArsI GACNNNNNNTTYG 2 cut(s) 669, 701
Asp700I GAANNNNTTC 1 cut(s) 619
AsuHPI GGTGA 4 cut(s) 199, 356, 574, 719
AsuII TTCGAA 2 cut(s) 501, 623
BbsI GAAGAC 1 cut(s) 97
BbvI GCAGC 4 cut(s) 47, 167, 214, 236
BccI CCATC 3 cut(s) 16, 74, 214
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 1 cut(s) 126
BclI TGATCA 1 cut(s) 300
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 33
BglII AGATCT 1 cut(s) 277
BisI GCNGC 5 cut(s) 36, 156, 225, 228, 231
BlsI GCNGC 5 cut(s) 37, 157, 226, 229, 232
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 3 cut(s) 47, 270, 565
BpiI GAAGAC 1 cut(s) 97
Bpu10I CCTNAGC 1 cut(s) 220
Bpu14I TTCGAA 2 cut(s) 501, 623
BsaBI GATNNNNATC 1 cut(s) 444
BsaJI CCNNGG 2 cut(s) 125, 150
BsaWI WCCGGW 2 cut(s) 242, 268
Bse3DI GCAATG 2 cut(s) 85, 139
Bse8I GATNNNNATC 1 cut(s) 444
BseAI TCCGGA 1 cut(s) 268
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 125, 150
BseGI GGATG 4 cut(s) 27, 261, 445, 556
BseJI GATNNNNATC 1 cut(s) 444
BseMI GCAATG 2 cut(s) 85, 139
BseMII CTCAG 2 cut(s) 117, 324
BseXI GCAGC 4 cut(s) 47, 167, 214, 236
BshFI GGCC 1 cut(s) 315
BsiSI CCGG 2 cut(s) 243, 269
BsnI GGCC 1 cut(s) 315
Bsp119I TTCGAA 2 cut(s) 501, 623
Bsp13I TCCGGA 1 cut(s) 268
Bsp143I GATC 4 cut(s) 82, 277, 300, 588
BspACI CCGC 2 cut(s) 231, 609
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 2 cut(s) 116, 323
BspEI TCCGGA 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 37
BspT104I TTCGAA 2 cut(s) 501, 623
BsrDI GCAATG 2 cut(s) 85, 139
BssECI CCNNGG 2 cut(s) 125, 150
BssMI GATC 4 cut(s) 82, 277, 300, 588
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 75
Bst6I CTCTTC 4 cut(s) 524, 610, 650, 695
BstAPI GCANNNNNTGC 1 cut(s) 41
BstBI TTCGAA 2 cut(s) 501, 623
BstC8I GCNNGC 1 cut(s) 160
BstDEI CTNAG 3 cut(s) 103, 220, 310
BstEII GGTNACC 2 cut(s) 344, 580
BstF5I GGATG 4 cut(s) 27, 261, 445, 556
BstKTI GATC 4 cut(s) 85, 280, 303, 591
BstMBI GATC 4 cut(s) 82, 277, 300, 588
BstMWI GCNNNNNNNGC 7 cut(s) 41, 50, 84, 155, 224, 230, 433
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 512
BstPI GGTNACC 2 cut(s) 344, 580
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 4 cut(s) 47, 167, 214, 236
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 1 cut(s) 315
BtsCI GGATG 4 cut(s) 27, 261, 445, 556
BtsIMutI CAGTG 2 cut(s) 111, 465
Cac8I GCNNGC 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 41
Csp6I GTAC 1 cut(s) 415
CviAII CATG 4 cut(s) 7, 163, 509, 586
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 3 cut(s) 103, 220, 310
DpnI GATC 4 cut(s) 84, 279, 302, 590
DpnII GATC 4 cut(s) 82, 277, 300, 588
Eam1104I CTCTTC 4 cut(s) 524, 610, 650, 695
EarI CTCTTC 4 cut(s) 524, 610, 650, 695
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 315
Eco57I CTGAAG 1 cut(s) 449
Eco91I GGTNACC 2 cut(s) 344, 580
EcoO65I GGTNACC 2 cut(s) 344, 580
EcoRI GAATTC 2 cut(s) 137, 332
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 4 cut(s) 10, 166, 512, 589
FaiI YATR 9 cut(s) 8, 17, 164, 179, 510, 551, 575, 587, 717
FatI CATG 4 cut(s) 6, 162, 508, 585
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 5 cut(s) 36, 156, 225, 228, 231
FokI GGATG 4 cut(s) 34, 248, 452, 543
Fsp4HI GCNGC 5 cut(s) 36, 156, 225, 228, 231
FspBI CTAG 1 cut(s) 281
GluI GCNGC 5 cut(s) 36, 156, 225, 228, 231
HaeIII GGCC 1 cut(s) 315
HapII CCGG 2 cut(s) 243, 269
Hin1II CATG 4 cut(s) 10, 166, 512, 589
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 51
HinfI GANTC 5 cut(s) 101, 287, 308, 498, 727
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 2 cut(s) 243, 269
HphI GGTGA 4 cut(s) 199, 356, 574, 719
Hpy166II GTNNAC 2 cut(s) 114, 417
Hpy188I TCNGA 2 cut(s) 450, 593
Hpy188III TCNNGA 4 cut(s) 269, 337, 541, 652
Hpy8I GTNNAC 2 cut(s) 114, 417
HpyAV CCTTC 3 cut(s) 275, 508, 517
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4V TGCA 5 cut(s) 35, 44, 78, 261, 402
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 50, 84, 155, 224, 230, 433
HpyF3I CTNAG 3 cut(s) 103, 220, 310
Hsp92II CATG 4 cut(s) 10, 166, 512, 589
Kpn2I TCCGGA 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 300
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 4 cut(s) 82, 277, 300, 588
LmnI GCTCC 2 cut(s) 129, 271
Lsp1109I GCAGC 4 cut(s) 47, 167, 214, 236
LweI GCATC 3 cut(s) 47, 270, 565
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 3 cut(s) 187, 344, 580
MalI GATC 4 cut(s) 84, 279, 302, 590
MboI GATC 4 cut(s) 82, 277, 300, 588
MboII GAAGA 7 cut(s) 102, 304, 541, 611, 627, 667, 712
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 277
MluCI AATT 5 cut(s) 137, 213, 251, 332, 367
MlyI GAGTC 2 cut(s) 95, 302
MnlI CCTC 8 cut(s) 12, 178, 239, 525, 537, 571, 651, 703
MroI TCCGGA 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 619
MseI TTAA 2 cut(s) 113, 444
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 2 cut(s) 243, 269
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 7 cut(s) 41, 50, 84, 155, 224, 230, 433
NdeII GATC 4 cut(s) 82, 277, 300, 588
NlaIII CATG 4 cut(s) 10, 166, 512, 589
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 3 cut(s) 187, 344, 580
NspI RCATGY 1 cut(s) 512
NspV TTCGAA 2 cut(s) 501, 623
PceI AGGCCT 1 cut(s) 315
PciI ACATGT 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 619
PfeI GAWTC 3 cut(s) 287, 498, 727
PkrI GCNGC 5 cut(s) 37, 157, 226, 229, 232
PleI GAGTC 2 cut(s) 95, 302
PpsI GAGTC 2 cut(s) 95, 302
PscI ACATGT 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 124
PspEI GGTNACC 2 cut(s) 344, 580
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 273
PstI CTGCAG 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SaqAI TTAA 2 cut(s) 113, 444
SatI GCNGC 5 cut(s) 36, 156, 225, 228, 231
Sau3AI GATC 4 cut(s) 82, 277, 300, 588
SchI GAGTC 2 cut(s) 95, 302
ScrFI CCNGG 1 cut(s) 126
SfaNI GCATC 3 cut(s) 47, 270, 565
SfcI CTRYAG 1 cut(s) 33
SfuI TTCGAA 2 cut(s) 501, 623
Sse9I AATT 5 cut(s) 137, 213, 251, 332, 367
SseBI AGGCCT 1 cut(s) 315
SsiI CCGC 2 cut(s) 231, 609
SspMI CTAG 1 cut(s) 281
StuI AGGCCT 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 75
TaqI TCGA 3 cut(s) 290, 501, 623
TasI AATT 5 cut(s) 137, 213, 251, 332, 367
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 3 cut(s) 287, 498, 727
Tru1I TTAA 2 cut(s) 113, 444
Tru9I TTAA 2 cut(s) 113, 444
TscAI CASTG 2 cut(s) 111, 465
TseFI GTSAC 3 cut(s) 187, 344, 580
TseI GCWGC 4 cut(s) 35, 155, 224, 227
Tsp45I GTSAC 3 cut(s) 187, 344, 580
TspDTI ATGAA 4 cut(s) 150, 179, 566, 642
TspRI CASTG 2 cut(s) 111, 465
XapI RAATTY 2 cut(s) 137, 332
XceI RCATGY 1 cut(s) 512
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 619
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.