RLG00000028079

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0019487
Physical Location & Seq
Reverse (-)
18368148 .. 18369710
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028079

Sequence Viewer

Length: 585 bp
ATGTCGAGTTCACCACCATCTCCGCCGGACGAGGAATGGACGCTGATCAATGAGGCGGCCGATTGGGGATGGAGATCTTGGCATTATGGAGATCTCCGTGATGTAGGAGATCGCTGCTCCCTTCTCCACTGTGTGGTCAGTCGGTCGGGTCCCATCAACCCTTACTTCACCAACTTTTCCCATCTCGATGAAGACGACGACGATGATGAAGAAGAAGAGAGGCAGCGACGAAAAGCTTCAGAGGTCTCAGCGACGCTGCCTTTATCGGTGACGTCGTTCTTATCAGCTATTCCGGCGCCTAGGAACTCGAGTGTGAGTTCCGGTTTAGGTTCCGGTAGGAGAGCCATTGTGGAAACAGAGTCATTTGGTTCTAAAGAGGAGACCAGTGTGGGTTTGGATAAGATGATCCCCAATCAAAATTTGATCCAAGTGAAGGTTGATAGTTACCAATATATTGTTGATCAGAATGTGGATTCTGGGTTTCAATTTCAAGGAGGTTCAGCTGGAAATAGGGATGATGATAAGGATTTAGCCTTCATAGGCTTGCTCATCCACTTTCGGTTGCATTTGTTGCTGCCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

195

Amino Acids

21.59

Weight (kDa)

4.56

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019487)

Species Orthologous Gene IDs
rosa_laevigata RLG00000028079 RLG00000029455
rosa_multiflora Rmu_sc0000918.1_g000003
rosa_samantha Rh1AG173400 Rh3DG081800 Rh4DG382300 Rh6AG370100 Rh7CG214700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 583
AatII GACGTC 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 295
AccB7I CCANNNNNTGG 1 cut(s) 133
AciI CCGC 2 cut(s) 23, 56
AclWI GGATC 2 cut(s) 400, 418
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 418
AcuI CTGAAG 1 cut(s) 222
AcyI GRCGYC 2 cut(s) 272, 296
AdeI CACNNNGTG 1 cut(s) 133
AfiI CCNNNNNNNGG 2 cut(s) 133, 433
AgsI TTSAA 2 cut(s) 485, 491
AluBI AGCT 3 cut(s) 236, 287, 503
AluI AGCT 3 cut(s) 236, 287, 503
Alw26I GTCTC 2 cut(s) 250, 374
AlwI GGATC 2 cut(s) 400, 418
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 4 cut(s) 114, 223, 256, 574
ApoI RAATTY 1 cut(s) 418
Asp700I GAANNNNTTC 1 cut(s) 235
AspA2I CCTAGG 1 cut(s) 299
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 3, 160, 280
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 149
AvrII CCTAGG 1 cut(s) 299
BanI GGYRCC 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 4 cut(s) 101, 235, 243, 561
BccI CCATC 4 cut(s) 25, 63, 161, 189
BclI TGATCA 2 cut(s) 45, 460
BcoDI GTCTC 2 cut(s) 250, 374
BfaI CTAG 1 cut(s) 300
BfoI RGCGCY 1 cut(s) 299
BglII AGATCT 2 cut(s) 74, 91
BisI GCNGC 5 cut(s) 57, 115, 224, 257, 575
BlnI CCTAGG 1 cut(s) 299
BlsI GCNGC 5 cut(s) 58, 116, 225, 258, 576
Bme18I GGWCC 1 cut(s) 149
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 4 cut(s) 150, 151, 297, 331
BpiI GAAGAC 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 73
BsaHI GRCGYC 2 cut(s) 272, 296
BsaI GGTCTC 2 cut(s) 250, 374
BsaJI CCNNGG 1 cut(s) 299
BsaWI WCCGGW 2 cut(s) 320, 332
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 433
Bse1I ACTGG 1 cut(s) 384
Bse8I GATNNNNATC 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 299
BseGI GGATG 3 cut(s) 74, 520, 549
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 2 cut(s) 133, 433
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 392
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 4 cut(s) 101, 235, 243, 561
Bsh1285I CGRYCG 2 cut(s) 60, 146
BshFI GGCC 1 cut(s) 59
BshNI GGYRCC 1 cut(s) 295
BsiEI CGRYCG 2 cut(s) 60, 146
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 4 cut(s) 26, 293, 321, 333
BslFI GGGAC 1 cut(s) 135
BslI CCNNNNNNNGG 2 cut(s) 133, 433
BsmAI GTCTC 2 cut(s) 250, 374
BsmFI GGGAC 1 cut(s) 135
BsnI GGCC 1 cut(s) 59
Bso31I GGTCTC 2 cut(s) 250, 374
BsoBI CYCGRG 1 cut(s) 307
Bsp143I GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
BspACI CCGC 2 cut(s) 23, 56
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 1 cut(s) 260
BspLI GGNNCC 4 cut(s) 150, 151, 297, 331
BspPI GGATC 2 cut(s) 400, 418
BspT107I GGYRCC 1 cut(s) 295
BspTNI GGTCTC 2 cut(s) 250, 374
BsrI ACTGG 1 cut(s) 384
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
BssNI GRCGYC 2 cut(s) 272, 296
BssT1I CCWWGG 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 2 cut(s) 272, 296
BstAPI GCANNNNNTGC 1 cut(s) 571
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 1 cut(s) 247
BstF5I GGATG 3 cut(s) 74, 520, 549
BstH2I RGCGCY 1 cut(s) 299
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 7 cut(s) 48, 77, 94, 112, 408, 426, 463
BstMAI GTCTC 2 cut(s) 250, 374
BstMBI GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
BstMCI CGRYCG 2 cut(s) 60, 146
BstMWI GCNNNNNNNGC 2 cut(s) 293, 571
BstV1I GCAGC 4 cut(s) 101, 235, 243, 561
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 2 cut(s) 74, 91
BstYI RGATCY 2 cut(s) 74, 91
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 1 cut(s) 59
BtsCI GGATG 3 cut(s) 74, 520, 549
BtsIMutI CAGTG 2 cut(s) 127, 391
Cac8I GCNNGC 1 cut(s) 545
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 149
CseI GACGC 2 cut(s) 49, 262
CviJI RGCY 7 cut(s) 59, 236, 287, 344, 503, 533, 543
CviKI_1 RGCY 7 cut(s) 59, 236, 287, 344, 503, 533, 543
DdeI CTNAG 1 cut(s) 247
DinI GGCGCC 1 cut(s) 297
DpnI GATC 7 cut(s) 47, 76, 93, 111, 407, 425, 462
DpnII GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
DraIII CACNNNGTG 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 12
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 299
Eco31I GGTCTC 2 cut(s) 250, 374
Eco47I GGWCC 1 cut(s) 149
Eco52I CGGCCG 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 307
EcoO109I RGGNCCY 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 299
EgeI GGCGCC 1 cut(s) 297
EheI GGCGCC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 299
FaiI YATR 4 cut(s) 87, 453, 539, 583
FaqI GGGAC 1 cut(s) 135
FbaI TGATCA 2 cut(s) 45, 460
Fnu4HI GCNGC 5 cut(s) 57, 115, 224, 257, 575
FokI GGATG 3 cut(s) 81, 527, 536
Fsp4HI GCNGC 5 cut(s) 57, 115, 224, 257, 575
FspBI CTAG 1 cut(s) 300
GlaI GCGC 1 cut(s) 297
GluI GCNGC 5 cut(s) 57, 115, 224, 257, 575
HaeII RGCGCY 1 cut(s) 299
HaeIII GGCC 1 cut(s) 59
HapII CCGG 4 cut(s) 26, 293, 321, 333
HgaI GACGC 2 cut(s) 49, 262
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 2 cut(s) 272, 296
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 234
HinfI GANTC 2 cut(s) 359, 473
HpaII CCGG 4 cut(s) 26, 293, 321, 333
HphI GGTGA 3 cut(s) 3, 160, 280
Hpy166II GTNNAC 1 cut(s) 11
Hpy188I TCNGA 2 cut(s) 241, 465
Hpy188III TCNNGA 1 cut(s) 185
Hpy8I GTNNAC 1 cut(s) 11
Hpy99I CGWCG 5 cut(s) 200, 203, 231, 256, 277
HpyAV CCTTC 3 cut(s) 131, 427, 544
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 1 cut(s) 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 571
HpyF3I CTNAG 1 cut(s) 247
HpySE526I ACGT 1 cut(s) 272
Hsp92I GRCGYC 2 cut(s) 272, 296
HspAI GCGC 1 cut(s) 296
KasI GGCGCC 1 cut(s) 295
KflI GGGWCCC 1 cut(s) 149
Ksp22I TGATCA 2 cut(s) 45, 460
Kzo9I GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 7 cut(s) 39, 306, 334, 346, 397, 462, 489
Lsp1109I GCAGC 4 cut(s) 101, 235, 243, 561
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 268, 443
MalI GATC 7 cut(s) 47, 76, 93, 111, 407, 425, 462
MboI GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
MboII GAAGA 4 cut(s) 203, 221, 224, 227
MflI RGATCY 2 cut(s) 74, 91
MluCI AATT 2 cut(s) 418, 485
Mly113I GGCGCC 1 cut(s) 296
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 6 cut(s) 25, 46, 213, 235, 370, 488
MroXI GAANNNNTTC 1 cut(s) 235
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 4 cut(s) 26, 293, 321, 333
MwoI GCNNNNNNNGC 2 cut(s) 293, 571
NarI GGCGCC 1 cut(s) 296
NdeII GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
NlaIV GGNNCC 4 cut(s) 150, 151, 297, 331
NmuCI GTSAC 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 307
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 272
PdmI GAANNNNTTC 1 cut(s) 235
PfeI GAWTC 1 cut(s) 473
PflMI CCANNNNNTGG 1 cut(s) 133
PkrI GCNGC 5 cut(s) 58, 116, 225, 258, 576
PleI GAGTC 1 cut(s) 367
PluTI GGCGCC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 367
PpuMI RGGWCCY 1 cut(s) 149
PsiI TTATAA 1 cut(s) 583
Psp5II RGGWCCY 1 cut(s) 149
PspN4I GGNNCC 4 cut(s) 150, 151, 297, 331
PspPI GGNCC 1 cut(s) 149
PspPPI RGGWCCY 1 cut(s) 149
PspXI VCTCGAGB 1 cut(s) 307
PsuI RGATCY 2 cut(s) 74, 91
PvuII CAGCTG 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 5 cut(s) 57, 115, 224, 257, 575
Sau3AI GATC 7 cut(s) 45, 74, 91, 109, 405, 423, 460
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 1 cut(s) 368
SetI ASST 8 cut(s) 238, 246, 275, 289, 331, 438, 499, 505
SfoI GGCGCC 1 cut(s) 297
Sfr274I CTCGAG 1 cut(s) 307
SinI GGWCC 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 307
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 2 cut(s) 418, 485
SsiI CCGC 2 cut(s) 23, 56
SspDI GGCGCC 1 cut(s) 295
SspMI CTAG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 1 cut(s) 275
TaqI TCGA 3 cut(s) 5, 186, 308
TaqII GACCGA 1 cut(s) 132
TasI AATT 2 cut(s) 418, 485
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 473
TscAI CASTG 2 cut(s) 134, 391
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 4 cut(s) 114, 223, 256, 574
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 204, 222, 526
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 2 cut(s) 134, 391
Van91I CCANNNNNTGG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 149
XapI RAATTY 1 cut(s) 418
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XhoI CTCGAG 1 cut(s) 307
XmaJI CCTAGG 1 cut(s) 299
XmnI GAANNNNTTC 1 cut(s) 235
XspI CTAG 1 cut(s) 300
ZraI GACGTC 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.