Rh7CG214700

Mitotic checkpoint regulator, MAD2B-interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0019487
Physical Location & Seq
Forward (+)
19422377 .. 19422694
318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG214700.1

Sequence Viewer

Length: 318 bp
ATGAAAGCTTCAGAGGTCTCATCGGCGCTGCCTTTGTCTGTGACGTCGTTCTTATCAGCTATTCCGGCGCCCAGGAACTCGGGTGTGAGTTCCAGTTTAGGTTCTGGTAGGAGAGCCATTGTGGAAACAGAGTCGTTTGGTTCTAAAGAGGAGACCAATATGGGTTTGGATCAGATGATCCCCAATCAAAATTTGATCCAAGTGAAGGTTGACAGTTACCAATATATTGTTGATCAGAATGTGGATTCTGGGTTTCAATTTCAAGGAGGTATAGTGGTTATGCTGGAAACCATGGGAACGAATGGGCTGATGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 ko05211 map05202 map05211
Pfam Domains
Protein Families
PRCC

Protein Analysis

105

Amino Acids

11.15

Weight (kDa)

4.5

Isoelectric Point (pI)

22.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019487)

Species Orthologous Gene IDs
rosa_laevigata RLG00000028079 RLG00000029455
rosa_multiflora Rmu_sc0000918.1_g000003
rosa_samantha Rh1AG173400 Rh3DG081800 Rh4DG382300 Rh6AG370100 Rh7CG214700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 67
AclWI GGATC 3 cut(s) 172, 177, 190
AcsI RAATTY 1 cut(s) 190
AcyI GRCGYC 2 cut(s) 44, 68
AfiI CCNNNNNNNGG 1 cut(s) 205
AgsI TTSAA 2 cut(s) 257, 263
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 2 cut(s) 8, 59
AluI AGCT 2 cut(s) 8, 59
Alw26I GTCTC 2 cut(s) 22, 146
AlwI GGATC 3 cut(s) 172, 177, 190
Ama87I CYCGRG 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 190
AspLEI GCGC 2 cut(s) 28, 70
AvaI CYCGRG 1 cut(s) 79
BanI GGYRCC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 15
BccI CCATC 1 cut(s) 304
BciT130I CCWGG 1 cut(s) 73
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 22, 146
BfoI RGCGCY 2 cut(s) 29, 71
BisI GCNGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 30
Bme1390I CCNGG 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 73
BsaHI GRCGYC 2 cut(s) 44, 68
BsaI GGTCTC 2 cut(s) 22, 146
BsaJI CCNNGG 2 cut(s) 71, 291
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 2 cut(s) 71, 291
BseLI CCNNNNNNNGG 1 cut(s) 205
BseNI ACTGG 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 1 cut(s) 15
BshNI GGYRCC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 1 cut(s) 205
BsmAI GTCTC 2 cut(s) 22, 146
Bso31I GGTCTC 2 cut(s) 22, 146
BsoBI CYCGRG 1 cut(s) 79
Bsp143I GATC 4 cut(s) 169, 177, 195, 232
Bsp19I CCATGG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 3 cut(s) 172, 177, 190
BspT107I GGYRCC 1 cut(s) 67
BspTNI GGTCTC 2 cut(s) 22, 146
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 71, 291
BssMI GATC 4 cut(s) 169, 177, 195, 232
BssNI GRCGYC 2 cut(s) 44, 68
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 215
BstACI GRCGYC 2 cut(s) 44, 68
BstDSI CCRYGG 1 cut(s) 291
BstH2I RGCGCY 2 cut(s) 29, 71
BstHHI GCGC 2 cut(s) 28, 70
BstKTI GATC 4 cut(s) 172, 180, 198, 235
BstMAI GTCTC 2 cut(s) 22, 146
BstMBI GATC 4 cut(s) 169, 177, 195, 232
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 291
CfoI GCGC 2 cut(s) 28, 70
CviAII CATG 1 cut(s) 292
CviJI RGCY 4 cut(s) 8, 59, 116, 307
CviKI_1 RGCY 4 cut(s) 8, 59, 116, 307
DinI GGCGCC 1 cut(s) 69
DpnI GATC 4 cut(s) 171, 179, 197, 234
DpnII GATC 4 cut(s) 169, 177, 195, 232
Eco130I CCWWGG 1 cut(s) 291
Eco31I GGTCTC 2 cut(s) 22, 146
Eco88I CYCGRG 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 291
EgeI GGCGCC 1 cut(s) 69
EheI GGCGCC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 1 cut(s) 295
FaiI YATR 5 cut(s) 161, 225, 272, 281, 293
FatI CATG 1 cut(s) 291
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 29
GlaI GCGC 2 cut(s) 27, 69
GluI GCNGC 1 cut(s) 29
HaeII RGCGCY 2 cut(s) 29, 71
HapII CCGG 1 cut(s) 65
HhaI GCGC 2 cut(s) 28, 70
Hin1I GRCGYC 2 cut(s) 44, 68
Hin1II CATG 1 cut(s) 295
Hin6I GCGC 2 cut(s) 26, 68
HinP1I GCGC 2 cut(s) 26, 68
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 2 cut(s) 131, 245
HpaII CCGG 1 cut(s) 65
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 13, 174, 237
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 2 cut(s) 44, 68
Hsp92II CATG 1 cut(s) 295
HspAI GCGC 2 cut(s) 26, 68
KasI GGCGCC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 4 cut(s) 169, 177, 195, 232
LpnPI CCDG 7 cut(s) 58, 78, 85, 90, 106, 234, 269
Lsp1109I GCAGC 1 cut(s) 15
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 2 cut(s) 40, 215
MalI GATC 4 cut(s) 171, 179, 197, 234
MboI GATC 4 cut(s) 169, 177, 195, 232
MluCI AATT 2 cut(s) 190, 257
Mly113I GGCGCC 1 cut(s) 68
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 3 cut(s) 7, 142, 260
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 1 cut(s) 65
NarI GGCGCC 1 cut(s) 68
NcoI CCATGG 1 cut(s) 291
NdeII GATC 4 cut(s) 169, 177, 195, 232
NlaIII CATG 1 cut(s) 295
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 1 cut(s) 30
PleI GAGTC 1 cut(s) 139
PluTI GGCGCC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 69
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 4 cut(s) 169, 177, 195, 232
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 73
SetI ASST 7 cut(s) 10, 18, 47, 61, 103, 210, 271
SfoI GGCGCC 1 cut(s) 69
Sse9I AATT 2 cut(s) 190, 257
SspDI GGCGCC 1 cut(s) 67
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 1 cut(s) 47
TasI AATT 2 cut(s) 190, 257
TfiI GAWTC 1 cut(s) 245
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 17
XapI RAATTY 1 cut(s) 190
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
ZraI GACGTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.