RLG00000029050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018561
Physical Location & Seq
Reverse (-)
31993763 .. 31996530
2768 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029050

Sequence Viewer

Length: 687 bp
ATGTGTAGTAAACGAAAACGAATCTGGGAACATGACCTGGTTGGCGGTGGACCAGTGGGTGGACCAGTGGGGTCCTCCAACCGACATGACAATGACAAGCCGAACAGCAAGGCTGCTGCCAGCCGTGGACTAGTGGATGGACCAGTGGGAAAAGAAACAAATGGAGAAGATGGCCTTGATAGTGGTGGGCCCGTGGGATTTGGAGAAGATGGATCCGTGGGATTTTGGCAAAGTAATTTTGAAAAGTCAAACAAGGAAGATGCAACATGCCATGACGTCAGAACATCTTCCCCAAGTGGTCTTGAAAAATCAAACAAGGATGGTCCTACGCGCCCGCAACATGGCCTGCATCATGAGAGTGACAATGATGCCTCAATGCTTCCTCGACTATCCGTGAAATTTGACTACTTTTTCCTTCAGCGAACACAGCTGCAACCCGCTCCGCCAGTCCGCCTCTCCGGTCTCCGAGCTCCGCCTTTAGGCTCTAGATTGCAGGAGGGACTGGTCAGATTGTCAGAGGAATCTTCATGTGTAGCAATGCTTATGCTAATCAGATTGCTTCTTGCTTACTGCCTTGGAGTTGGAGATCTAATTCCATGTCTGAAGCATCTGCTAGCTCCTCTCACCCATCCGCTTCCAATTTTGAACCAGCCGCTTCCAGCAGCTGCATTGTTTAAATTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

24.5

Weight (kDa)

6.71

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018561)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0347231 RchiOBHm_Chr2g0148441
rosa_laevigata RLG00000029050
rosa_roxburghii Rroxscaffold_6G00419110
rosa_samantha Rh2AG478400 Rh2CG464600 Rh2DG555200 Rh3CG254100 Rh5DG281200 Rh7DG158900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB7I CCANNNNNTGG 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 440
AccII CGCG 1 cut(s) 331
AciI CCGC 8 cut(s) 45, 335, 438, 443, 451, 473, 632, 653
AclWI GGATC 2 cut(s) 207, 220
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 2 cut(s) 401, 623
AcyI GRCGYC 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 59, 341, 479
AgsI TTSAA 3 cut(s) 242, 305, 646
AhlI ACTAGT 1 cut(s) 130
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 4 cut(s) 430, 470, 617, 665
AluI AGCT 4 cut(s) 430, 470, 617, 665
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 2 cut(s) 207, 220
AlwNI CAGNNNCTG 1 cut(s) 665
AoxI GGCC 3 cut(s) 172, 188, 343
ApaI GGGCCC 1 cut(s) 192
ApeKI GCWGC 5 cut(s) 113, 116, 430, 662, 665
ApoI RAATTY 1 cut(s) 398
Asp700I GAANNNNTTC 1 cut(s) 286
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 7 cut(s) 50, 62, 72, 140, 188, 189, 323
AsuHPI GGTGA 1 cut(s) 616
AsuNHI GCTAGC 1 cut(s) 613
AvaII GGWCC 5 cut(s) 50, 62, 72, 140, 323
BaeGI GKGCMC 1 cut(s) 192
BamHI GGATCC 1 cut(s) 212
BanII GRGCYC 2 cut(s) 192, 472
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 5 cut(s) 100, 103, 417, 652, 674
BccI CCATC 5 cut(s) 131, 164, 203, 314, 636
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 467
BcuI ACTAGT 1 cut(s) 130
BfaI CTAG 3 cut(s) 131, 486, 614
BglII AGATCT 1 cut(s) 586
BisI GCNGC 6 cut(s) 114, 117, 431, 653, 663, 666
BlsI GCNGC 6 cut(s) 115, 118, 432, 654, 664, 667
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 5 cut(s) 50, 62, 72, 140, 323
BmgT120I GGNCC 7 cut(s) 50, 62, 72, 140, 188, 189, 323
BmiI GGNNCC 3 cut(s) 73, 190, 214
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 4 cut(s) 250, 358, 358, 616
BmtI GCTAGC 1 cut(s) 617
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 124, 192, 216, 574
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 341, 479
Bse1I ACTGG 5 cut(s) 53, 65, 143, 446, 507
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 4 cut(s) 124, 192, 216, 574
BseGI GGATG 3 cut(s) 142, 325, 628
BseLI CCNNNNNNNGG 3 cut(s) 59, 341, 479
BseMI GCAATG 1 cut(s) 543
BseNI ACTGG 5 cut(s) 53, 65, 143, 446, 507
BseRI GAGGAG 1 cut(s) 609
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 5 cut(s) 100, 103, 417, 652, 674
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 3 cut(s) 174, 190, 345
BsiHKAI GWGCWC 1 cut(s) 472
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 3 cut(s) 59, 341, 479
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 513
BsnI GGCC 3 cut(s) 174, 190, 345
Bso31I GGTCTC 1 cut(s) 467
Bsp120I GGGCCC 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 192, 472
Bsp143I GATC 2 cut(s) 212, 586
BspACI CCGC 8 cut(s) 45, 335, 438, 443, 451, 473, 632, 653
BspANI GGCC 3 cut(s) 174, 190, 345
BspFNI CGCG 1 cut(s) 331
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 3 cut(s) 73, 190, 214
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 2 cut(s) 207, 220
BspTNI GGTCTC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 543
BsrI ACTGG 5 cut(s) 53, 65, 143, 446, 507
BssECI CCNNGG 4 cut(s) 124, 192, 216, 574
BssMI GATC 2 cut(s) 212, 586
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 574
Bst2UI CCWGG 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 276
BstC8I GCNNGC 4 cut(s) 121, 335, 347, 615
BstDSI CCRYGG 3 cut(s) 124, 192, 216
BstF5I GGATG 3 cut(s) 142, 325, 628
BstFNI CGCG 1 cut(s) 331
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 2 cut(s) 215, 589
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 2 cut(s) 212, 586
BstMWI GCNNNNNNNGC 1 cut(s) 427
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 5 cut(s) 100, 103, 417, 652, 674
BstX2I RGATCY 2 cut(s) 212, 586
BstYI RGATCY 2 cut(s) 212, 586
BsuRI GGCC 3 cut(s) 174, 190, 345
BtgI CCRYGG 3 cut(s) 124, 192, 216
BtsCI GGATG 3 cut(s) 142, 325, 628
BtsIMutI CAGTG 3 cut(s) 60, 72, 150
Cac8I GCNNGC 4 cut(s) 121, 335, 347, 615
CaiI CAGNNNCTG 1 cut(s) 665
CciI TCATGA 1 cut(s) 352
CfoI GCGC 1 cut(s) 333
Cfr13I GGNCC 7 cut(s) 50, 62, 72, 140, 188, 189, 323
CsiI ACCWGGT 1 cut(s) 36
CviAII CATG 8 cut(s) 32, 86, 267, 272, 341, 353, 528, 597
DpnI GATC 2 cut(s) 214, 588
DpnII GATC 2 cut(s) 212, 586
DraI TTTAAA 1 cut(s) 676
EciI GGCGGA 3 cut(s) 432, 440, 462
Ecl136II GAGCTC 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 574
Eco24I GRGCYC 2 cut(s) 192, 472
Eco31I GGTCTC 1 cut(s) 467
Eco47I GGWCC 5 cut(s) 50, 62, 72, 140, 323
Eco53kI GAGCTC 1 cut(s) 470
Eco57I CTGAAG 2 cut(s) 401, 623
EcoICRI GAGCTC 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 574
EcoT38I GRGCYC 2 cut(s) 192, 472
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 8 cut(s) 35, 89, 270, 275, 344, 356, 531, 600
FaiI YATR 9 cut(s) 33, 87, 268, 273, 342, 354, 529, 545, 598
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 513
FatI CATG 8 cut(s) 31, 85, 266, 271, 340, 352, 527, 596
FauI CCCGC 2 cut(s) 342, 445
Fnu4HI GCNGC 6 cut(s) 114, 117, 431, 653, 663, 666
FokI GGATG 3 cut(s) 149, 332, 615
FriOI GRGCYC 2 cut(s) 192, 472
Fsp4HI GCNGC 6 cut(s) 114, 117, 431, 653, 663, 666
FspBI CTAG 3 cut(s) 131, 486, 614
GlaI GCGC 1 cut(s) 332
GluI GCNGC 6 cut(s) 114, 117, 431, 653, 663, 666
HaeIII GGCC 3 cut(s) 174, 190, 345
HapII CCGG 1 cut(s) 459
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 8 cut(s) 35, 89, 270, 275, 344, 356, 531, 600
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HinfI GANTC 2 cut(s) 21, 521
HpaII CCGG 1 cut(s) 459
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 4 cut(s) 11, 50, 62, 128
Hpy188I TCNGA 6 cut(s) 281, 467, 509, 517, 554, 603
Hpy188III TCNNGA 3 cut(s) 302, 353, 486
Hpy8I GTNNAC 4 cut(s) 11, 50, 62, 128
HpyAV CCTTC 1 cut(s) 425
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 5 cut(s) 263, 349, 433, 493, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 8 cut(s) 35, 89, 270, 275, 344, 356, 531, 600
HspAI GCGC 1 cut(s) 331
Kzo9I GATC 2 cut(s) 212, 586
LmnI GCTCC 3 cut(s) 445, 475, 622
Lsp1109I GCAGC 5 cut(s) 100, 103, 417, 652, 674
LweI GCATC 4 cut(s) 250, 358, 358, 616
MabI ACCWGGT 1 cut(s) 36
MaeI CTAG 3 cut(s) 131, 486, 614
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 359
MalI GATC 2 cut(s) 214, 588
MbiI CCGCTC 1 cut(s) 440
MboI GATC 2 cut(s) 212, 586
MboII GAAGA 5 cut(s) 179, 218, 269, 279, 516
MflI RGATCY 2 cut(s) 212, 586
MhlI GDGCHC 2 cut(s) 192, 472
MluCI AATT 5 cut(s) 235, 398, 591, 639, 677
MmeI TCCRAC 2 cut(s) 102, 562
MnlI CCTC 7 cut(s) 85, 382, 393, 464, 490, 511, 630
MroXI GAANNNNTTC 1 cut(s) 286
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 2 cut(s) 90, 357
MspA1I CMGCKG 2 cut(s) 430, 665
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 1 cut(s) 427
NdeII GATC 2 cut(s) 212, 586
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 8 cut(s) 35, 89, 270, 275, 344, 356, 531, 600
NlaIV GGNNCC 3 cut(s) 73, 190, 214
NmuCI GTSAC 1 cut(s) 359
NspI RCATGY 1 cut(s) 270
PagI TCATGA 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 286
PfeI GAWTC 2 cut(s) 21, 521
PflMI CCANNNNNTGG 1 cut(s) 59
PkrI GCNGC 6 cut(s) 115, 118, 432, 654, 664, 667
PpuMI RGGWCCY 1 cut(s) 72
Psp124BI GAGCTC 1 cut(s) 472
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 3 cut(s) 73, 190, 214
PspOMI GGGCCC 1 cut(s) 188
PspPI GGNCC 7 cut(s) 50, 62, 72, 140, 188, 189, 323
PspPPI RGGWCCY 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 2 cut(s) 212, 586
PvuII CAGCTG 2 cut(s) 430, 665
RseI CAYNNNNRTG 2 cut(s) 90, 357
SacI GAGCTC 1 cut(s) 472
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 6 cut(s) 114, 117, 431, 653, 663, 666
Sau3AI GATC 2 cut(s) 212, 586
Sau96I GGNCC 7 cut(s) 50, 62, 72, 140, 188, 189, 323
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 192, 472
SetI ASST 6 cut(s) 39, 279, 432, 472, 619, 667
SexAI ACCWGGT 1 cut(s) 36
SfaNI GCATC 4 cut(s) 250, 358, 358, 616
SinI GGWCC 5 cut(s) 50, 62, 72, 140, 323
SmiMI CAYNNNNRTG 2 cut(s) 90, 357
SpeI ACTAGT 1 cut(s) 130
Sse9I AATT 5 cut(s) 235, 398, 591, 639, 677
SsiI CCGC 8 cut(s) 45, 335, 438, 443, 451, 473, 632, 653
SspMI CTAG 3 cut(s) 131, 486, 614
SstI GAGCTC 1 cut(s) 472
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 574
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 385
TasI AATT 5 cut(s) 235, 398, 591, 639, 677
TauI GCSGC 1 cut(s) 655
TfiI GAWTC 2 cut(s) 21, 521
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TscAI CASTG 3 cut(s) 60, 72, 150
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 5 cut(s) 113, 116, 430, 662, 665
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 1 cut(s) 516
TspGWI ACGGA 2 cut(s) 205, 382
TspRI CASTG 3 cut(s) 60, 72, 150
Van91I CCANNNNNTGG 1 cut(s) 59
VpaK11BI GGWCC 5 cut(s) 50, 62, 72, 140, 323
XapI RAATTY 1 cut(s) 398
XbaI TCTAGA 1 cut(s) 485
XceI RCATGY 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 286
XspI CTAG 3 cut(s) 131, 486, 614
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.