RLG00000030041

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018802
Physical Location & Seq
Reverse (-)
53812791 .. 53816930
4140 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030041

Sequence Viewer

Length: 2172 bp
ATGTTAGAATTGCACGGGTTCTCTGCACGGAGGGGAATTGATATTCTCGTCAATAAGTCTCTCATATCAATTTCAGGGGACAATCATCTAGAGATGCATGATTTGCTGCAAGAAATGGGTCAGTCCATCGTTCAAGAACAATCCACACATAACCTTGGAAAGCGCAGCAGGTTGTGGAATGCCAAGGATGTCTATCAAGTATTGAAAAATAGGATGGGAACTACAAAACTCCAAGCCATCATATCTACCATTCCGGAGTTACAACTGAGTCCTGACACCTTCAAAAATATGCACAATCTAAGGTTGCTAAAATTTTCTGCACAGCCAAATGTGGACAACAAAATGCACTTTCCTGAACTTGAGCTTCACATGCCCAATGGCAAAGTTAAGAAGCTTTCGCATAATAATGGCCAGAAATTTGGGAACATGAAAAAGTTGAATCTTAGAGATTGCCGACACCTGACTGAAGTTCCGGATTTCTCTCAGTGTCCAAATATTGAAAAATTAATTCTCACTGGTTGTACAGGTTTGGTTAACCTGTATGTTAAAAAGTTTGACAAGCTTACTACTCTGAAACTAGCAAGCTGCAGGAGTCTTAAAATTTATCCTGAGATACCATGCAATGTGCAACACTTAAATTTGTCTTGCTCTAAGCTGACTGAAGTTCCCGATCTCTCTCGGTGTCCAAATATTGAGAAAATATATCTGTATCGTTGTGCGAAGTTGGTTCAACTTCCTAATGGATATCTTAAACATCTTGCCAAGCTTACTACTCTTAATATGGCAGACTGTTCAAGTCTTAAAATTCCTCCTCAGATACCATGCGGTTTACGAATCTTAGATTTGTCTTCCTGCTCTCAACTGACTGAAGTTCCTGATCTCTCTCAGTGTCCAAATATTGAGGCAATAGATCTCTGTAGGTGTACAAGTTTGGTTCAACTCCCTAGCTCAATTTTTAAAAATCTTGACAAGCTTACTCGTCTGCAGCTGGCAAGGTGCTCGAGTCTTAAAGCTTCTCCTGAGTTACCACGCAATTTGCGACAATTGGATTTGTCTTCTTGCTCTCAGTTGACTGAAGTTCCTGATCTCTCTCGGTGTCCAAATATTGAGGAGATAGATCTCTGTAGGTGTACAAGTTTGGTTCAACTCCCTAACTCAATTTTTAAAAATCTTGACAAGCTTACTCGTCTGCAGCTGGCAAGGTGCTCTAGTCTTAAAGTTTGTCCCGAGTTACCACGCAATACACGATATTTGAACTTGTCCTATTGCTCTCAACTGATCGAAGTTCCCGATCTCTCTGGCTGCAGTCGAAACCTTGAGGAAATAGATCTCGGTGATTGTACAAGTTTGGTGCAGATCCCTGATTCATATGTTAGAAATCTTGATAAGCTTACTTCTCTGTATCTGCAAAACTGCTCGAGTCTTAAAGTCTTCCCGAAAATACCACAAAATGTACGAAACTTGCATGCACCTTCTTGCTCTCAGCTGGCTGAATTTCCTGATATCTCTGGGTGTCCTAATATTGAGAGAATAGATCTTAGTCACTGTATAAGTTTGTTTCATGTCCCTCCATATGTCAAAAATCTTGATTTCTTTCGGTATCCAGATATTAAGATAAATCTTCTTGGTTGCACAAGTTTGGACCAACCCCCTTCAATCATACGCAGAGCTTCTGAGTCATTCCCAGTAACTCCGAGTCACATGCAGGAGATGGAGAACATGATCCAGTTGCAGCAGGAGGCGGACATGATCCAATTGCGTAAGTTTGCTGACTTTGGTTTGGCCAATCAAAGTTCAGAAGGGCACACACGCTTGACTAAAAGAGTTGCTGATCATGAGTGGGAGAAAATGCTGGACACATCGAACTCCAGCACGCATTTGAACTCTGTTGTTTTGATCACAGATTGGGCGTGGATATATAAGAACTCTGGGAGCGTGGAGGAGGTTCTTGATGAGTCATTGAGGGAGGAAGGACCAAAGGCTGTGATGGAGAGGCTTGTAAATGTTGGGATTCTTAGTGCTCATGTTATGGTGGCTTTTAGGCCTACCATTTCAGAAGCACTGAGGATGTTGGAGGATGATATTGATATTCAAAATTACCAGATTGGCCAATTCCACTTGGGAATGAGTCTTATAGATCTTCTTTTGGGTTTTCTAGCTTCACTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

724

Amino Acids

81.83

Weight (kDa)

7.45

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 5 - 48 4.2e-11 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018802)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g27710
pyrus_communis pycom08g07010
rosa_chinensis RchiOBHm_Chr7g0203781
rosa_laevigata RLG00000030041 RLG00000030745
rosa_multiflora Rmu_sc0000795.1_g000055 Rmu_sc0001802.1_g000007 Rmu_sc0002194.1_g000020
rosa_samantha Rh7BG294500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 159
AccIII TCCGGA 2 cut(s) 253, 472
AciI CCGC 2 cut(s) 825, 1742
AclWI GGATC 3 cut(s) 1351, 1717, 1744
AcoI YGGCCR 3 cut(s) 409, 1782, 2107
AcsI RAATTY 6 cut(s) 311, 416, 600, 637, 804, 1493
AcuI CTGAAG 4 cut(s) 486, 681, 888, 1095
AfaI GTAC 5 cut(s) 523, 925, 1132, 1342, 1455
Alw21I GWGCWC 3 cut(s) 1001, 1208, 2023
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 3 cut(s) 1351, 1717, 1744
Ama87I CYCGRG 3 cut(s) 1000, 1226, 1417
Aor13HI TCCGGA 2 cut(s) 253, 472
AoxI GGCC 4 cut(s) 409, 1782, 2042, 2107
ApeKI GCWGC 7 cut(s) 106, 165, 585, 985, 1192, 1302, 1732
ApoI RAATTY 6 cut(s) 311, 416, 600, 637, 804, 1493
AseI ATTAAT 1 cut(s) 506
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 1642, 1973
AsuHPI GGTGA 1 cut(s) 1346
AvaI CYCGRG 3 cut(s) 1000, 1226, 1417
AvaII GGWCC 2 cut(s) 1642, 1973
BaeGI GKGCMC 1 cut(s) 1806
BalI TGGCCA 3 cut(s) 411, 1784, 2109
BbsI GAAGAC 3 cut(s) 840, 1047, 1423
Bbv12I GWGCWC 3 cut(s) 1001, 1208, 2023
BbvI GCAGC 7 cut(s) 93, 177, 572, 997, 1204, 1289, 1744
BccI CCATC 5 cut(s) 134, 208, 245, 1705, 1981
BcgI CGANNNNNNTGC 4 cut(s) 981, 1015, 1684, 1718
BciVI GTATCC 1 cut(s) 1611
BclI TGATCA 2 cut(s) 1831, 1896
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 5 cut(s) 89, 578, 945, 1209, 2156
BfmI CTRYAG 6 cut(s) 586, 916, 983, 1123, 1190, 1303
BfuAI ACCTGC 1 cut(s) 159
BfuI GTATCC 1 cut(s) 1611
BglII AGATCT 5 cut(s) 910, 1117, 1327, 1534, 2137
BisI GCNGC 7 cut(s) 107, 166, 586, 986, 1193, 1303, 1733
BlsI GCNGC 7 cut(s) 108, 167, 587, 987, 1194, 1304, 1734
Bme18I GGWCC 2 cut(s) 1642, 1973
BmeT110I CYCGRG 3 cut(s) 1000, 1226, 1417
BmgT120I GGNCC 2 cut(s) 1642, 1973
BmrI ACTGGG 1 cut(s) 1679
BmsI GCATC 1 cut(s) 84
BmuI ACTGGG 1 cut(s) 1679
BpiI GAAGAC 3 cut(s) 840, 1047, 1423
BpmI CTGGAG 1 cut(s) 1852
BpuEI CTTGAG 2 cut(s) 380, 1337
BsaBI GATNNNNATC 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 154, 183
BsaWI WCCGGW 2 cut(s) 253, 472
Bse1I ACTGG 3 cut(s) 520, 1685, 1726
Bse3DI GCAATG 1 cut(s) 628
Bse8I GATNNNNATC 1 cut(s) 192
BseAI TCCGGA 2 cut(s) 253, 472
BseDI CCNNGG 2 cut(s) 154, 183
BseGI GGATG 4 cut(s) 193, 219, 2073, 2083
BseJI GATNNNNATC 1 cut(s) 192
BseMI GCAATG 1 cut(s) 628
BseNI ACTGG 3 cut(s) 520, 1685, 1726
BseRI GAGGAG 3 cut(s) 801, 1124, 1955
BseSI GKGCMC 1 cut(s) 1806
BseXI GCAGC 7 cut(s) 93, 177, 572, 997, 1204, 1289, 1744
BsgI GTGCAG 3 cut(s) 9, 303, 1373
BshFI GGCC 4 cut(s) 411, 1784, 2044, 2109
BsiHKAI GWGCWC 3 cut(s) 1001, 1208, 2023
BsiHKCI CYCGRG 3 cut(s) 1000, 1226, 1417
BsiSI CCGG 2 cut(s) 254, 473
BslFI GGGAC 3 cut(s) 92, 1209, 1550
BsmAI GTCTC 1 cut(s) 63
BsmFI GGGAC 3 cut(s) 92, 1209, 1550
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 4 cut(s) 411, 1784, 2044, 2109
BsoBI CYCGRG 3 cut(s) 1000, 1226, 1417
Bsp1286I GDGCHC 4 cut(s) 1001, 1208, 1806, 2023
Bsp13I TCCGGA 2 cut(s) 253, 472
Bsp1407I TGTACA 4 cut(s) 521, 923, 1130, 1340
BspACI CCGC 2 cut(s) 825, 1742
BspANI GGCC 4 cut(s) 411, 1784, 2044, 2109
BspEI TCCGGA 2 cut(s) 253, 472
BspHI TCATGA 1 cut(s) 1834
BspMAI CTGCAG 4 cut(s) 590, 987, 1194, 1307
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 3 cut(s) 1351, 1717, 1744
BsrDI GCAATG 1 cut(s) 628
BsrGI TGTACA 4 cut(s) 521, 923, 1130, 1340
BsrI ACTGG 3 cut(s) 520, 1685, 1726
BssECI CCNNGG 2 cut(s) 154, 183
BssT1I CCWWGG 2 cut(s) 154, 183
Bst4CI ACNGT 2 cut(s) 791, 1547
BstAPI GCANNNNNTGC 1 cut(s) 103
BstAUI TGTACA 4 cut(s) 521, 923, 1130, 1340
BstC8I GCNNGC 6 cut(s) 583, 990, 1197, 1467, 1488, 1874
BstF5I GGATG 4 cut(s) 193, 219, 2073, 2083
BstHHI GCGC 1 cut(s) 165
BstMAI GTCTC 1 cut(s) 63
BstMWI GCNNNNNNNGC 2 cut(s) 103, 370
BstNSI RCATGY 3 cut(s) 373, 1469, 1705
BstSFI CTRYAG 6 cut(s) 586, 916, 983, 1123, 1190, 1303
BstSLI GKGCMC 1 cut(s) 1806
BstV1I GCAGC 7 cut(s) 93, 177, 572, 997, 1204, 1289, 1744
BstV2I GAAGAC 3 cut(s) 840, 1047, 1423
BstX2I RGATCY 6 cut(s) 910, 1117, 1327, 1356, 1534, 2137
BstXI CCANNNNNNTGG 1 cut(s) 419
BstYI RGATCY 6 cut(s) 910, 1117, 1327, 1356, 1534, 2137
BsuI GTATCC 1 cut(s) 1611
BsuRI GGCC 4 cut(s) 411, 1784, 2044, 2109
BtsCI GGATG 4 cut(s) 193, 219, 2073, 2083
BtsIMutI CAGTG 5 cut(s) 491, 513, 893, 1543, 2060
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 6 cut(s) 583, 990, 1197, 1467, 1488, 1874
CciI TCATGA 1 cut(s) 1834
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 1642, 1973
Csp6I GTAC 5 cut(s) 522, 924, 1131, 1341, 1454
CspCI CAANNNNNGTGG 2 cut(s) 1017, 1052
CviQI GTAC 5 cut(s) 522, 924, 1131, 1341, 1454
DraI TTTAAA 2 cut(s) 958, 1165
EaeI YGGCCR 3 cut(s) 409, 1782, 2107
EciI GGCGGA 1 cut(s) 1757
Eco130I CCWWGG 2 cut(s) 154, 183
Eco147I AGGCCT 1 cut(s) 2044
Eco32I GATATC 2 cut(s) 746, 1504
Eco47I GGWCC 2 cut(s) 1642, 1973
Eco57I CTGAAG 4 cut(s) 486, 681, 888, 1095
Eco88I CYCGRG 3 cut(s) 1000, 1226, 1417
EcoRV GATATC 2 cut(s) 746, 1504
EcoT14I CCWWGG 2 cut(s) 154, 183
EcoT22I ATGCAT 1 cut(s) 99
ErhI CCWWGG 2 cut(s) 154, 183
FaqI GGGAC 3 cut(s) 92, 1209, 1550
FauNDI CATATG 2 cut(s) 1369, 1573
FbaI TGATCA 2 cut(s) 1831, 1896
Fnu4HI GCNGC 7 cut(s) 107, 166, 586, 986, 1193, 1303, 1733
FokI GGATG 4 cut(s) 200, 226, 2080, 2090
Fsp4HI GCNGC 7 cut(s) 107, 166, 586, 986, 1193, 1303, 1733
FspBI CTAG 5 cut(s) 89, 578, 945, 1209, 2156
GlaI GCGC 1 cut(s) 164
GluI GCNGC 7 cut(s) 107, 166, 586, 986, 1193, 1303, 1733
GsuI CTGGAG 1 cut(s) 1852
HaeIII GGCC 4 cut(s) 411, 1784, 2044, 2109
HapII CCGG 2 cut(s) 254, 473
HhaI GCGC 1 cut(s) 165
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 2 cut(s) 535, 1071
HindII GTYRAC 2 cut(s) 535, 1071
HindIII AAGCTT 7 cut(s) 392, 560, 764, 971, 1011, 1178, 1388
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 2 cut(s) 254, 473
HphI GGTGA 1 cut(s) 1346
Hpy166II GTNNAC 6 cut(s) 334, 535, 830, 924, 1071, 1131
Hpy188I TCNGA 6 cut(s) 573, 816, 1675, 1695, 1798, 2056
Hpy8I GTNNAC 6 cut(s) 334, 535, 830, 924, 1071, 1131
HpyAV CCTTC 5 cut(s) 289, 1482, 1662, 1793, 1964
HpyCH4III ACNGT 2 cut(s) 791, 1547
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 370
HspAI GCGC 1 cut(s) 163
Kpn2I TCCGGA 2 cut(s) 253, 472
Ksp22I TGATCA 2 cut(s) 1831, 1896
KspAI GTTAAC 1 cut(s) 535
LmnI GCTCC 1 cut(s) 1932
Lsp1109I GCAGC 7 cut(s) 93, 177, 572, 997, 1204, 1289, 1744
LweI GCATC 1 cut(s) 84
MaeI CTAG 5 cut(s) 89, 578, 945, 1209, 2156
MaeIII GTNAC 6 cut(s) 258, 1023, 1230, 1541, 1687, 1697
MboII GAAGA 5 cut(s) 840, 1047, 1423, 1613, 2132
MfeI CAATTG 2 cut(s) 1043, 1754
MflI RGATCY 6 cut(s) 910, 1117, 1327, 1356, 1534, 2137
MhlI GDGCHC 4 cut(s) 1001, 1208, 1806, 2023
MlsI TGGCCA 3 cut(s) 411, 1784, 2109
MluNI TGGCCA 3 cut(s) 411, 1784, 2109
MlyI GAGTC 8 cut(s) 277, 601, 1012, 1429, 1685, 1705, 1964, 2137
MmeI TCCRAC 1 cut(s) 2052
Mox20I TGGCCA 3 cut(s) 411, 1784, 2109
Mph1103I ATGCAT 1 cut(s) 99
MroI TCCGGA 2 cut(s) 253, 472
MscI TGGCCA 3 cut(s) 411, 1784, 2109
MslI CAYNNNNRTG 1 cut(s) 405
Msp20I TGGCCA 3 cut(s) 411, 1784, 2109
MspA1I CMGCKG 3 cut(s) 988, 1195, 1486
MspI CCGG 2 cut(s) 254, 473
MunI CAATTG 2 cut(s) 1043, 1754
Mva1269I GAATGC 1 cut(s) 184
MwoI GCNNNNNNNGC 2 cut(s) 103, 370
NdeI CATATG 2 cut(s) 1369, 1573
NmuCI GTSAC 2 cut(s) 1541, 1697
NsiI ATGCAT 1 cut(s) 99
NspI RCATGY 3 cut(s) 373, 1469, 1705
PaeI GCATGC 1 cut(s) 1469
PaeR7I CTCGAG 2 cut(s) 1000, 1417
PagI TCATGA 1 cut(s) 1834
PceI AGGCCT 1 cut(s) 2044
PcsI WCGNNNNNNNCGW 3 cut(s) 1036, 1243, 1287
PctI GAATGC 1 cut(s) 184
PfeI GAWTC 4 cut(s) 439, 834, 1364, 2011
PkrI GCNGC 7 cut(s) 108, 167, 587, 987, 1194, 1304, 1734
PleI GAGTC 8 cut(s) 276, 600, 1011, 1428, 1684, 1704, 1963, 2136
PpsI GAGTC 8 cut(s) 276, 600, 1011, 1428, 1684, 1704, 1963, 2136
PshBI ATTAAT 1 cut(s) 506
PspPI GGNCC 2 cut(s) 1642, 1973
PspXI VCTCGAGB 2 cut(s) 1000, 1417
PstI CTGCAG 4 cut(s) 590, 987, 1194, 1307
PsuI RGATCY 6 cut(s) 910, 1117, 1327, 1356, 1534, 2137
PvuII CAGCTG 3 cut(s) 988, 1195, 1486
RsaI GTAC 5 cut(s) 523, 925, 1132, 1342, 1455
RsaNI GTAC 5 cut(s) 522, 924, 1131, 1341, 1454
RseI CAYNNNNRTG 1 cut(s) 405
SatI GCNGC 7 cut(s) 107, 166, 586, 986, 1193, 1303, 1733
Sau96I GGNCC 2 cut(s) 1642, 1973
SchI GAGTC 8 cut(s) 277, 601, 1012, 1429, 1685, 1705, 1964, 2137
SduI GDGCHC 4 cut(s) 1001, 1208, 1806, 2023
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 6 cut(s) 586, 916, 983, 1123, 1190, 1303
Sfr274I CTCGAG 2 cut(s) 1000, 1417
SinI GGWCC 2 cut(s) 1642, 1973
SlaI CTCGAG 2 cut(s) 1000, 1417
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 4 cut(s) 359, 1000, 1316, 1417
SmoI CTYRAG 4 cut(s) 359, 1000, 1316, 1417
SphI GCATGC 1 cut(s) 1469
SseBI AGGCCT 1 cut(s) 2044
SsiI CCGC 2 cut(s) 825, 1742
SspI AATATT 5 cut(s) 496, 691, 898, 1105, 1522
SspMI CTAG 5 cut(s) 89, 578, 945, 1209, 2156
StuI AGGCCT 1 cut(s) 2044
StyI CCWWGG 2 cut(s) 154, 183
TaaI ACNGT 2 cut(s) 791, 1547
TaqI TCGA 5 cut(s) 1001, 1281, 1309, 1418, 1862
TatI WGTACW 4 cut(s) 521, 923, 1130, 1340
TfiI GAWTC 4 cut(s) 439, 834, 1364, 2011
TscAI CASTG 5 cut(s) 491, 520, 893, 1550, 2067
TseFI GTSAC 2 cut(s) 1541, 1697
TseI GCWGC 7 cut(s) 106, 165, 585, 985, 1192, 1302, 1732
Tsp45I GTSAC 2 cut(s) 1541, 1697
TspDTI ATGAA 3 cut(s) 443, 1356, 1550
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 5 cut(s) 491, 520, 893, 1550, 2067
VpaK11BI GGWCC 2 cut(s) 1642, 1973
VspI ATTAAT 1 cut(s) 506
XapI RAATTY 6 cut(s) 311, 416, 600, 637, 804, 1493
XbaI TCTAGA 1 cut(s) 88
XceI RCATGY 3 cut(s) 373, 1469, 1705
XhoI CTCGAG 2 cut(s) 1000, 1417
XspI CTAG 5 cut(s) 89, 578, 945, 1209, 2156
Zsp2I ATGCAT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.