Rmu_sc0002194.1_g000020

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002194.1
OG0018802
Physical Location & Seq
Reverse (-)
75004 .. 76873
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002194.1_g000020.1.cds

Sequence Viewer

Length: 981 bp
atggctcttgtaggagagcttcttggaggggctgttctagggacggtagttacagatctctatactcgtataaaggatctgatcagcaggtctaagcagtttgaaccgctcctgagagatatccaatccaagctagaatcactgcagccaatcattgaagagataagtaaacataatatcaaattgaatctcccaaacgtggttatagaaagctgcaaaaagcaaatggaggagggcataaagctggttggcgagttctcgagtgctggtaacaagttcaatttcaagaagcgcaagtataccaaacaactacaggaattggatgcatctcttaaacaattcttggctatacttgatgtacaaggactaaaggatgggaaagagaccttgggtttggttagagaattaacagatgtgcacgtcgagaggatcctcaagcaaatacgaacaaatgatgaagatccacagcttacagttggatcggatgtgaaatcgatgctcattgttgaaggaaacagggaaaccacattgaagaaactagatccatttcacttggagcgagtcaaagggatgaggctgaattggggtgagttgatttggtgggttgagtcgatttggtggagttggttgactcaaagcctcaaacaatcaaactcatctctcgattcaaaaaccagaaactcacctcaaatccccgagggtggtgccgacgtaggcgcactttttgactcccacaacgttgagaatgttgagcaagtcgtcgacgaaggcgtccagaacgatgcgatcgtaaggtttgaagaaaggaagaatgcgaccccaggcggactggtcggtgccgaagccatgggcaaggaccaggtactgctcgcccgacccggttacccggacattcagagcttccaagattctagtgttgctgggacactagaccaggcagtgacgggacagcgacgacgagggcacggggaggcggtgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

326

Amino Acids

36.41

Weight (kDa)

5.72

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018802)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g27710
pyrus_communis pycom08g07010
rosa_chinensis RchiOBHm_Chr7g0203781
rosa_laevigata RLG00000030041 RLG00000030745
rosa_multiflora Rmu_sc0000795.1_g000055 Rmu_sc0001802.1_g000007 Rmu_sc0002194.1_g000020
rosa_samantha Rh7BG294500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 770
Acc36I ACCTGC 1 cut(s) 78
AccB1I GGYRCC 2 cut(s) 704, 836
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 2 cut(s) 299, 762
AciI CCGC 3 cut(s) 107, 825, 974
AclI AACGTT 1 cut(s) 738
AclWI GGATC 6 cut(s) 84, 424, 437, 455, 487, 536
AcyI GRCGYC 1 cut(s) 771
AfaI GTAC 2 cut(s) 360, 864
AfiI CCNNNNNNNGG 2 cut(s) 199, 701
AgsI TTSAA 9 cut(s) 104, 158, 187, 280, 286, 509, 532, 669, 800
AjiI CACGTC 1 cut(s) 421
AjnI CCWGG 3 cut(s) 820, 858, 933
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AloI GAACNNNNNNTCC 2 cut(s) 18, 50
AluBI AGCT 6 cut(s) 19, 133, 213, 244, 469, 900
AluI AGCT 6 cut(s) 19, 133, 213, 244, 469, 900
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 1 cut(s) 377
Alw44I GTGCAC 1 cut(s) 416
AlwI GGATC 6 cut(s) 84, 424, 437, 455, 487, 536
AlwNI CAGNNNCTG 1 cut(s) 865
Ama87I CYCGRG 2 cut(s) 259, 695
ApaLI GTGCAC 1 cut(s) 416
ApeKI GCWGC 2 cut(s) 145, 213
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
AspLEI GCGC 2 cut(s) 294, 719
AspS9I GGNCC 1 cut(s) 856
AsuC2I CCSGG 2 cut(s) 879, 887
AsuHPI GGTGA 2 cut(s) 599, 675
AvaI CYCGRG 2 cut(s) 259, 695
AvaII GGWCC 1 cut(s) 856
BaeGI GKGCMC 2 cut(s) 420, 966
BamHI GGATCC 1 cut(s) 429
BanI GGYRCC 2 cut(s) 704, 836
BarI GAAGNNNNNNTAC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 2 cut(s) 157, 200
BccI CCATC 1 cut(s) 368
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 3 cut(s) 822, 860, 935
BclI TGATCA 1 cut(s) 81
BcnI CCSGG 2 cut(s) 879, 887
BcoDI GTCTC 1 cut(s) 377
BfaI CTAG 5 cut(s) 38, 134, 539, 912, 929
BfmI CTRYAG 2 cut(s) 143, 311
BfuAI ACCTGC 1 cut(s) 78
BglII AGATCT 1 cut(s) 55
BisI GCNGC 2 cut(s) 146, 214
BlsI GCNGC 2 cut(s) 147, 215
Bme1390I CCNGG 5 cut(s) 822, 860, 879, 887, 935
Bme18I GGWCC 1 cut(s) 856
BmeT110I CYCGRG 2 cut(s) 259, 695
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 856
BmiI GGNNCC 3 cut(s) 431, 706, 838
BmrFI CCNGG 5 cut(s) 822, 860, 879, 887, 935
BmsI GCATC 4 cut(s) 313, 335, 486, 772
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 2 cut(s) 879, 887
Bsa29I ATCGAT 1 cut(s) 494
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 771
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 4 cut(s) 387, 696, 820, 846
BsaXI ACNNNNNCTCC 3 cut(s) 18, 48, 962
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 701
Bse1I ACTGG 1 cut(s) 834
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 3 cut(s) 822, 860, 935
BseCI ATCGAT 1 cut(s) 494
BseDI CCNNGG 4 cut(s) 387, 696, 820, 846
BseGI GGATG 4 cut(s) 328, 379, 490, 576
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 199, 701
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 1 cut(s) 834
BseRI GAGGAG 1 cut(s) 245
BseSI GKGCMC 2 cut(s) 420, 966
BseXI GCAGC 2 cut(s) 157, 200
BseYI CCCAGC 1 cut(s) 920
Bsh1285I CGRYCG 1 cut(s) 789
BshNI GGYRCC 2 cut(s) 704, 836
BshVI ATCGAT 1 cut(s) 494
BsiEI CGRYCG 1 cut(s) 789
BsiHKAI GWGCWC 1 cut(s) 420
BsiHKCI CYCGRG 2 cut(s) 259, 695
BsiSI CCGG 2 cut(s) 879, 887
BslFI GGGAC 3 cut(s) 55, 937, 960
BslI CCNNNNNNNGG 2 cut(s) 199, 701
BsmAI GTCTC 1 cut(s) 377
BsmFI GGGAC 3 cut(s) 55, 937, 960
BsmI GAATGC 1 cut(s) 817
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 2 cut(s) 259, 695
Bsp1286I GDGCHC 2 cut(s) 420, 966
Bsp1407I TGTACA 1 cut(s) 358
Bsp143I GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 3 cut(s) 107, 825, 974
BspCNI CTCAG 1 cut(s) 105
BspDI ATCGAT 1 cut(s) 494
BspLI GGNNCC 3 cut(s) 431, 706, 838
BspMAI CTGCAG 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 78
BspPI GGATC 6 cut(s) 84, 424, 437, 455, 487, 536
BspT107I GGYRCC 2 cut(s) 704, 836
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 109
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 1 cut(s) 834
BssECI CCNNGG 4 cut(s) 387, 696, 820, 846
BssMI GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
BssNAI GTATAC 1 cut(s) 300
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 2 cut(s) 387, 846
Bst1107I GTATAC 1 cut(s) 300
Bst2UI CCWGG 3 cut(s) 822, 860, 935
Bst4CI ACNGT 2 cut(s) 46, 475
Bst6I CTCTTC 1 cut(s) 153
BstACI GRCGYC 1 cut(s) 771
BstAUI TGTACA 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 870
BstDEI CTNAG 2 cut(s) 93, 113
BstDSI CCRYGG 1 cut(s) 846
BstEII GGTNACC 1 cut(s) 881
BstF5I GGATG 4 cut(s) 328, 379, 490, 576
BstHHI GCGC 2 cut(s) 294, 719
BstKTI GATC 8 cut(s) 58, 79, 84, 432, 463, 482, 544, 789
BstMAI GTCTC 1 cut(s) 377
BstMBI GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
BstMCI CGRYCG 1 cut(s) 789
BstNI CCWGG 3 cut(s) 822, 860, 935
BstPI GGTNACC 1 cut(s) 881
BstSCI CCNGG 5 cut(s) 820, 858, 877, 885, 933
BstSFI CTRYAG 2 cut(s) 143, 311
BstSLI GKGCMC 2 cut(s) 420, 966
BstV1I GCAGC 2 cut(s) 157, 200
BstX2I RGATCY 5 cut(s) 55, 76, 429, 460, 541
BstYI RGATCY 5 cut(s) 55, 76, 429, 460, 541
BstZ17I GTATAC 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 494
BsuTUI ATCGAT 1 cut(s) 494
BtgI CCRYGG 1 cut(s) 846
BtrI CACGTC 1 cut(s) 421
BtsCI GGATG 4 cut(s) 328, 379, 490, 576
BtsI GCAGTG 2 cut(s) 140, 945
BtsIMutI CAGTG 2 cut(s) 140, 945
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 870
CaiI CAGNNNCTG 1 cut(s) 865
CfoI GCGC 2 cut(s) 294, 719
Cfr13I GGNCC 1 cut(s) 856
ClaI ATCGAT 1 cut(s) 494
CseI GACGC 1 cut(s) 760
CsiI ACCWGGT 1 cut(s) 858
Csp6I GTAC 2 cut(s) 359, 863
CviAII CATG 1 cut(s) 847
CviQI GTAC 2 cut(s) 359, 863
DdeI CTNAG 2 cut(s) 93, 113
DpnI GATC 8 cut(s) 57, 78, 83, 431, 462, 481, 543, 788
DpnII GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
EciI GGCGGA 1 cut(s) 840
Eco130I CCWWGG 2 cut(s) 387, 846
Eco31I GGTCTC 1 cut(s) 377
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 856
Eco88I CYCGRG 2 cut(s) 259, 695
Eco91I GGTNACC 1 cut(s) 881
EcoO65I GGTNACC 1 cut(s) 881
EcoRII CCWGG 3 cut(s) 820, 858, 933
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 2 cut(s) 387, 846
EcoT22I ATGCAT 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 387, 846
FaeI CATG 1 cut(s) 850
FaiI YATR 8 cut(s) 63, 71, 174, 206, 239, 300, 350, 848
FaqI GGGAC 3 cut(s) 55, 937, 960
FatI CATG 1 cut(s) 846
FbaI TGATCA 1 cut(s) 81
FblI GTMKAC 2 cut(s) 299, 762
Fnu4HI GCNGC 2 cut(s) 146, 214
FokI GGATG 4 cut(s) 335, 386, 497, 583
Fsp4HI GCNGC 2 cut(s) 146, 214
FspBI CTAG 5 cut(s) 38, 134, 539, 912, 929
GlaI GCGC 2 cut(s) 293, 718
GluI GCNGC 2 cut(s) 146, 214
GsaI CCCAGC 1 cut(s) 924
HapII CCGG 2 cut(s) 879, 887
HgaI GACGC 1 cut(s) 760
HhaI GCGC 2 cut(s) 294, 719
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 1 cut(s) 850
Hin6I GCGC 2 cut(s) 292, 717
HinP1I GCGC 2 cut(s) 292, 717
HincII GTYRAC 2 cut(s) 630, 763
HindII GTYRAC 2 cut(s) 630, 763
HinfI GANTC 8 cut(s) 137, 187, 561, 608, 631, 665, 728, 908
HpaII CCGG 2 cut(s) 879, 887
HphI GGTGA 2 cut(s) 599, 675
Hpy166II GTNNAC 5 cut(s) 170, 300, 418, 630, 763
Hpy188I TCNGA 3 cut(s) 81, 484, 897
Hpy188III TCNNGA 6 cut(s) 112, 259, 286, 424, 662, 775
Hpy8I GTNNAC 5 cut(s) 170, 300, 418, 630, 763
Hpy99I CGWCG 6 cut(s) 425, 713, 764, 767, 957, 960
HpyAV CCTTC 2 cut(s) 503, 761
HpyCH4III ACNGT 2 cut(s) 46, 475
HpyCH4IV ACGT 4 cut(s) 198, 420, 711, 738
HpyCH4V TGCA 4 cut(s) 145, 216, 326, 418
HpyF3I CTNAG 2 cut(s) 93, 113
HpySE526I ACGT 4 cut(s) 198, 420, 711, 738
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 1 cut(s) 850
HspAI GCGC 2 cut(s) 292, 717
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
LmnI GCTCC 2 cut(s) 114, 556
Lsp1109I GCAGC 2 cut(s) 157, 200
LweI GCATC 4 cut(s) 313, 335, 486, 772
MabI ACCWGGT 1 cut(s) 858
MaeI CTAG 5 cut(s) 38, 134, 539, 912, 929
MaeII ACGT 4 cut(s) 198, 420, 711, 738
MaeIII GTNAC 4 cut(s) 49, 269, 881, 940
MalI GATC 8 cut(s) 57, 78, 83, 431, 462, 481, 543, 788
MbiI CCGCTC 1 cut(s) 109
MboI GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
MboII GAAGA 5 cut(s) 170, 470, 544, 812, 820
MflI RGATCY 5 cut(s) 55, 76, 429, 460, 541
MhlI GDGCHC 2 cut(s) 420, 966
MluCI AATT 6 cut(s) 182, 280, 317, 338, 404, 580
MlyI GAGTC 4 cut(s) 570, 617, 625, 722
MmeI TCCRAC 1 cut(s) 457
Mph1103I ATGCAT 1 cut(s) 328
MseI TTAA 2 cut(s) 333, 407
MspI CCGG 2 cut(s) 879, 887
MspR9I CCNGG 5 cut(s) 822, 860, 879, 887, 935
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 3 cut(s) 822, 860, 935
NciI CCSGG 2 cut(s) 879, 887
NcoI CCATGG 1 cut(s) 846
NdeII GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
NlaIII CATG 1 cut(s) 850
NlaIV GGNNCC 3 cut(s) 431, 706, 838
NmuCI GTSAC 1 cut(s) 940
NsiI ATGCAT 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 259
PcsI WCGNNNNNNNCGW 2 cut(s) 768, 786
PctI GAATGC 1 cut(s) 817
PfeI GAWTC 4 cut(s) 137, 187, 665, 908
PkrI GCNGC 2 cut(s) 147, 215
Ple19I CGATCG 1 cut(s) 789
PleI GAGTC 4 cut(s) 569, 616, 625, 722
PpsI GAGTC 4 cut(s) 569, 616, 625, 722
Psp1406I AACGTT 1 cut(s) 738
Psp6I CCWGG 3 cut(s) 820, 858, 933
PspEI GGTNACC 1 cut(s) 881
PspFI CCCAGC 1 cut(s) 920
PspGI CCWGG 3 cut(s) 820, 858, 933
PspN4I GGNNCC 3 cut(s) 431, 706, 838
PspPI GGNCC 1 cut(s) 856
PstI CTGCAG 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 865
PsuI RGATCY 5 cut(s) 55, 76, 429, 460, 541
PvuI CGATCG 1 cut(s) 789
RsaI GTAC 2 cut(s) 360, 864
RsaNI GTAC 2 cut(s) 359, 863
SalI GTCGAC 1 cut(s) 761
SaqAI TTAA 2 cut(s) 333, 407
SatI GCNGC 2 cut(s) 146, 214
Sau3AI GATC 8 cut(s) 55, 76, 81, 429, 460, 479, 541, 786
Sau96I GGNCC 1 cut(s) 856
SchI GAGTC 4 cut(s) 570, 617, 625, 722
ScrFI CCNGG 5 cut(s) 822, 860, 879, 887, 935
SduI GDGCHC 2 cut(s) 420, 966
SexAI ACCWGGT 1 cut(s) 858
SfaNI GCATC 4 cut(s) 313, 335, 486, 772
SfcI CTRYAG 2 cut(s) 143, 311
Sfr274I CTCGAG 1 cut(s) 259
SgrDI CGTCGACG 1 cut(s) 761
SinI GGWCC 1 cut(s) 856
SlaI CTCGAG 1 cut(s) 259
SmlI CTYRAG 2 cut(s) 259, 434
SmoI CTYRAG 2 cut(s) 259, 434
Sse9I AATT 6 cut(s) 182, 280, 317, 338, 404, 580
SsiI CCGC 3 cut(s) 107, 825, 974
SspMI CTAG 5 cut(s) 38, 134, 539, 912, 929
StyD4I CCNGG 5 cut(s) 820, 858, 877, 885, 933
StyI CCWWGG 2 cut(s) 387, 846
TaaI ACNGT 2 cut(s) 46, 475
TaiI ACGT 4 cut(s) 201, 423, 714, 741
TaqI TCGA 6 cut(s) 260, 423, 494, 611, 663, 762
TasI AATT 6 cut(s) 182, 280, 317, 338, 404, 580
TatI WGTACW 1 cut(s) 358
TfiI GAWTC 4 cut(s) 137, 187, 665, 908
Tru1I TTAA 2 cut(s) 333, 407
Tru9I TTAA 2 cut(s) 333, 407
TscAI CASTG 2 cut(s) 147, 945
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 2 cut(s) 145, 213
Tsp45I GTSAC 1 cut(s) 940
TspDTI ATGAA 1 cut(s) 471
TspRI CASTG 2 cut(s) 147, 945
VneI GTGCAC 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 856
XhoI CTCGAG 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 299, 762
XspI CTAG 5 cut(s) 38, 134, 539, 912, 929
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.