RLG00000031557

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019414
Physical Location & Seq
Forward (+)
6181602 .. 6185202
3601 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031557

Sequence Viewer

Length: 768 bp
ATGGGTGTATTTGGGTCTAGACGCTTTGCCCGCTGTTTCTCTTCTAATGAGAGTATTGTCAACCGATTAGGGTTTTATCAGGAATTAGATGGGCATAATATTGAGAGTGCAGTCTCAGCAGAATTTAACTCCACAGGCAACCTTCTTGTGTCAGGTGGTGATCAGAAACAAATTATTGTTTGGGATTGGGAATCTAAAACTAGAAAATTCTCTTATGATTCTGGTCGCTTCAGATTAAATGTTACCAAGATGATGCCTCTCAGTGATGACAAGAGAATAGCAGCTGATTGTTGGGATGGTGAAGTGAGGCTAGGTGAAATCTTCTGTGGTGAGGATGGCTTCGTTCGACAGTTTGATCTGCGAAGCAGTTCTTCTAGAGAACTAGTGCATTGCTCCCGGCTGTCAAAGGGAAAACAAAACCCCCGGCAAATGGCGTTGAGTGATATAGTAATCGACCCAAGGAATCCATATTATTTTTGCATAGGAGGTACCGATGCATGTGCACGTGTCTATGACATCCGAAAAAATCAGCTCCAGGGAGATGAGCCAGTGGACATATTTTGCCCAGACCACCTGGTTGAGACGAGCAGGAGTTGGGTCCCTGGGTTGGCTTACTCCAAGTCAGGTGAACTAGCTATGAAATACTATAATGATCTCATTTATATGTTCAACAAGAATATGGAAGACATTGGCAAGCCGCAAAGCCAAATTTTGAGCGGGCGTTACTCTCACTCAAAGACCTTCTTCGACCTCCAAACCAAACGCTAG

Protein Analysis

256

Amino Acids

29.16

Weight (kDa)

7.56

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 28 - 125 6.2e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019414)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0008271 RchiOBHm_Chr5g0008341
rosa_laevigata RLG00000031557 RLG00000031562
rosa_roxburghii Rroxscaffold_1G00066830 Rroxscaffold_1G00067530 Rroxscaffold_1G00068190
rosa_samantha Rh5BG059700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 488
AccBSI CCGCTC 1 cut(s) 717
AciI CCGC 3 cut(s) 31, 698, 717
AcsI RAATTY 3 cut(s) 122, 206, 708
AcuI CTGAAG 1 cut(s) 214
AcvI CACGTG 1 cut(s) 506
AfaI GTAC 1 cut(s) 490
AfiI CCNNNNNNNGG 2 cut(s) 430, 607
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 1 cut(s) 670
AhlI ACTAGT 1 cut(s) 382
AjnI CCWGG 3 cut(s) 534, 573, 601
AluBI AGCT 3 cut(s) 284, 532, 635
AluI AGCT 3 cut(s) 284, 532, 635
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 2 cut(s) 118, 575
Alw44I GTGCAC 1 cut(s) 501
ApaLI GTGCAC 1 cut(s) 501
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 3 cut(s) 122, 206, 708
Asp700I GAANNNNTTC 1 cut(s) 367
Asp718I GGTACC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 598
AsuC2I CCSGG 2 cut(s) 397, 424
AsuHPI GGTGA 5 cut(s) 170, 311, 326, 341, 638
AvaII GGWCC 1 cut(s) 598
BaeGI GKGCMC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 488
BbrPI CACGTG 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 690
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 1 cut(s) 293
BccI CCATC 3 cut(s) 83, 290, 329
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 3 cut(s) 536, 575, 603
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 2 cut(s) 397, 424
BcoDI GTCTC 2 cut(s) 118, 575
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 7 cut(s) 18, 201, 311, 375, 383, 632, 766
BisI GCNGC 2 cut(s) 282, 698
BlsI GCNGC 2 cut(s) 283, 699
Bme1390I CCNGG 5 cut(s) 397, 424, 536, 575, 603
Bme18I GGWCC 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 3 cut(s) 490, 599, 600
BmrFI CCNGG 5 cut(s) 397, 424, 536, 575, 603
BmsI GCATC 2 cut(s) 243, 484
BpiI GAAGAC 1 cut(s) 690
BpmI CTGGAG 1 cut(s) 518
BpuMI CCSGG 2 cut(s) 397, 424
BsaAI YACGTR 1 cut(s) 506
BsaJI CCNNGG 5 cut(s) 422, 458, 535, 601, 602
Bsc4I CCNNNNNNNGG 2 cut(s) 430, 607
Bse1I ACTGG 1 cut(s) 548
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 3 cut(s) 536, 575, 603
BseDI CCNNGG 5 cut(s) 422, 458, 535, 601, 602
BseGI GGATG 3 cut(s) 301, 340, 516
BseLI CCNNNNNNNGG 2 cut(s) 430, 607
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 129, 274
BseNI ACTGG 1 cut(s) 548
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 488
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 2 cut(s) 397, 424
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 2 cut(s) 430, 607
BsmAI GTCTC 2 cut(s) 118, 575
BsmBI CGTCTC 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 3 cut(s) 160, 355, 652
BspACI CCGC 3 cut(s) 31, 698, 717
BspCNI CTCAG 2 cut(s) 128, 273
BspLI GGNNCC 3 cut(s) 490, 599, 600
BspT107I GGYRCC 1 cut(s) 488
BsrBI CCGCTC 1 cut(s) 717
BsrDI GCAATG 1 cut(s) 388
BsrI ACTGG 1 cut(s) 548
BssECI CCNNGG 5 cut(s) 422, 458, 535, 601, 602
BssMI GATC 3 cut(s) 160, 355, 652
BssT1I CCWWGG 1 cut(s) 458
Bst2UI CCWGG 3 cut(s) 536, 575, 603
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 1 cut(s) 46
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 31, 695, 719
BstDEI CTNAG 2 cut(s) 115, 260
BstF5I GGATG 3 cut(s) 301, 340, 516
BstKTI GATC 3 cut(s) 163, 358, 655
BstMAI GTCTC 2 cut(s) 118, 575
BstMBI GATC 3 cut(s) 160, 355, 652
BstMWI GCNNNNNNNGC 2 cut(s) 30, 116
BstNI CCWGG 3 cut(s) 536, 575, 603
BstNSI RCATGY 1 cut(s) 501
BstSCI CCNGG 5 cut(s) 395, 422, 534, 573, 601
BstSLI GKGCMC 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 293
BstV2I GAAGAC 1 cut(s) 690
BtsCI GGATG 3 cut(s) 301, 340, 516
BtsIMutI CAGTG 2 cut(s) 268, 555
Cac8I GCNNGC 3 cut(s) 31, 695, 719
Cfr13I GGNCC 1 cut(s) 598
CseI GACGC 1 cut(s) 30
CsiI ACCWGGT 1 cut(s) 573
Csp6I GTAC 1 cut(s) 489
CviAII CATG 1 cut(s) 498
CviQI GTAC 1 cut(s) 489
DdeI CTNAG 2 cut(s) 115, 260
DpnI GATC 3 cut(s) 162, 357, 654
DpnII GATC 3 cut(s) 160, 355, 652
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 214
Eco72I CACGTG 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 3 cut(s) 534, 573, 601
EcoT14I CCWWGG 1 cut(s) 458
EcoT22I ATGCAT 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 458
Esp3I CGTCTC 1 cut(s) 575
FaeI CATG 1 cut(s) 501
FalI AAGNNNNNCTT 4 cut(s) 355, 387, 728, 760
FaqI GGGAC 1 cut(s) 584
FatI CATG 1 cut(s) 497
FauI CCCGC 2 cut(s) 38, 710
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 282, 698
FokI GGATG 3 cut(s) 308, 347, 503
Fsp4HI GCNGC 2 cut(s) 282, 698
FspBI CTAG 7 cut(s) 18, 201, 311, 375, 383, 632, 766
GluI GCNGC 2 cut(s) 282, 698
GsuI CTGGAG 1 cut(s) 518
HapII CCGG 2 cut(s) 397, 424
HgaI GACGC 1 cut(s) 30
Hin1II CATG 1 cut(s) 501
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 3 cut(s) 191, 218, 463
HpaII CCGG 2 cut(s) 397, 424
HphI GGTGA 5 cut(s) 170, 311, 326, 341, 638
Hpy166II GTNNAC 4 cut(s) 61, 503, 553, 629
Hpy188I TCNGA 3 cut(s) 165, 233, 521
Hpy188III TCNNGA 3 cut(s) 18, 80, 375
Hpy8I GTNNAC 4 cut(s) 61, 503, 553, 629
HpyAV CCTTC 2 cut(s) 152, 751
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 1 cut(s) 505
HpyCH4V TGCA 5 cut(s) 110, 388, 480, 497, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 116
HpyF3I CTNAG 2 cut(s) 115, 260
HpySE526I ACGT 1 cut(s) 505
Hsp92II CATG 1 cut(s) 501
KflI GGGWCCC 1 cut(s) 598
KpnI GGTACC 1 cut(s) 492
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 3 cut(s) 160, 355, 652
LmnI GCTCC 2 cut(s) 398, 537
Lsp1109I GCAGC 1 cut(s) 293
LweI GCATC 2 cut(s) 243, 484
MabI ACCWGGT 1 cut(s) 573
MaeI CTAG 7 cut(s) 18, 201, 311, 375, 383, 632, 766
MaeII ACGT 1 cut(s) 505
MaeIII GTNAC 2 cut(s) 241, 722
MalI GATC 3 cut(s) 162, 357, 654
MbiI CCGCTC 1 cut(s) 717
MboI GATC 3 cut(s) 160, 355, 652
MboII GAAGA 5 cut(s) 33, 313, 363, 695, 736
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 5 cut(s) 83, 122, 171, 206, 708
MnlI CCTC 5 cut(s) 267, 300, 325, 479, 761
Mph1103I ATGCAT 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 367
MseI TTAA 2 cut(s) 126, 236
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 2 cut(s) 33, 284
MspI CCGG 2 cut(s) 397, 424
MspR9I CCNGG 5 cut(s) 397, 424, 536, 575, 603
MvaI CCWGG 3 cut(s) 536, 575, 603
MwoI GCNNNNNNNGC 2 cut(s) 30, 116
NciI CCSGG 2 cut(s) 397, 424
NdeII GATC 3 cut(s) 160, 355, 652
NlaIII CATG 1 cut(s) 501
NlaIV GGNNCC 3 cut(s) 490, 599, 600
NsiI ATGCAT 1 cut(s) 499
NspI RCATGY 1 cut(s) 501
PasI CCCWGGG 1 cut(s) 602
PdmI GAANNNNTTC 1 cut(s) 367
PfeI GAWTC 3 cut(s) 191, 218, 463
PkrI GCNGC 2 cut(s) 283, 699
PmaCI CACGTG 1 cut(s) 506
PmlI CACGTG 1 cut(s) 506
Ppu21I YACGTR 1 cut(s) 506
PpuMI RGGWCCY 1 cut(s) 598
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 3 cut(s) 534, 573, 601
PspCI CACGTG 1 cut(s) 506
PspGI CCWGG 3 cut(s) 534, 573, 601
PspN4I GGNNCC 3 cut(s) 490, 599, 600
PspPI GGNCC 1 cut(s) 598
PspPPI RGGWCCY 1 cut(s) 598
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 1 cut(s) 490
RsaNI GTAC 1 cut(s) 489
RseI CAYNNNNRTG 1 cut(s) 662
SaqAI TTAA 2 cut(s) 126, 236
SatI GCNGC 2 cut(s) 282, 698
Sau3AI GATC 3 cut(s) 160, 355, 652
Sau96I GGNCC 1 cut(s) 598
ScrFI CCNGG 5 cut(s) 397, 424, 536, 575, 603
SduI GDGCHC 1 cut(s) 505
SexAI ACCWGGT 1 cut(s) 573
SfaNI GCATC 2 cut(s) 243, 484
SinI GGWCC 1 cut(s) 598
SmiMI CAYNNNNRTG 1 cut(s) 662
SpeI ACTAGT 1 cut(s) 382
Sse9I AATT 5 cut(s) 83, 122, 171, 206, 708
SsiI CCGC 3 cut(s) 31, 698, 717
SspI AATATT 1 cut(s) 100
SspMI CTAG 7 cut(s) 18, 201, 311, 375, 383, 632, 766
StyD4I CCNGG 5 cut(s) 395, 422, 534, 573, 601
StyI CCWWGG 1 cut(s) 458
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 1 cut(s) 508
TaqI TCGA 3 cut(s) 346, 453, 747
TasI AATT 5 cut(s) 83, 122, 171, 206, 708
TauI GCSGC 1 cut(s) 700
TfiI GAWTC 3 cut(s) 191, 218, 463
Tru1I TTAA 2 cut(s) 126, 236
Tru9I TTAA 2 cut(s) 126, 236
TscAI CASTG 2 cut(s) 268, 555
TseI GCWGC 1 cut(s) 281
TspDTI ATGAA 1 cut(s) 653
TspRI CASTG 2 cut(s) 268, 555
VneI GTGCAC 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 598
XapI RAATTY 3 cut(s) 122, 206, 708
XbaI TCTAGA 2 cut(s) 17, 374
XceI RCATGY 1 cut(s) 501
XmnI GAANNNNTTC 1 cut(s) 367
XspI CTAG 7 cut(s) 18, 201, 311, 375, 383, 632, 766
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.