RLG00000031794

Peroxisome biogenesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015051
Physical Location & Seq
Forward (+)
8283204 .. 8284166
963 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031794

Sequence Viewer

Length: 963 bp
ATGCCGGTTTTCAAAACCCCATTCAACGGCTACTCCGTCAAGTTCAGCCCCTTCTACGAGTCCCGCCTCGCCGTCGCCACCTCCCAAAACTTCGGCATCCTCGGCAACGGCCGTCTCCACGTCATCGACCTCTCCCCCGCGGCCCCGGTCCCCACCCCCGTCACCGAGCTCGTCTCCTTCGACACCGCCGACGGCGTCTACGACGTCGCCTGGTCCGAGTCTCACGACTCCCTCCTCGTTGCCGCCATCGCCGACGGCTCCGTCAAGCTCTACGACCTCGCCCTCCCCCCGACCTCCAACCCTCTCCGCTCCCTCCACGAGCACACGCGCGAGGTCAGCTCCGCCGACTACAACCCCACGCGCCGCGACTCGTTCCTCACTGCCTCCTGGGACGACACCGTCAAGCTCTGGACCGTCGACCGCCCCGCCTCCGTCCGCACCTTCAAGGAGCACGCCTACTGCGTCTACTCCGCCGTCTGGAACCCCCGCCACGCCGACGTCTTCGCCTCCGCCTCCGGCGACTGCACCGCACGCGTCTGGGACGTGCGTGAGCCCGGATCCACCATGATCATCCCGGCGCACGAGTTCGAGATCCTCTCCTGCGATTGGAACAAGTACGACGACTGCGTCATCGCCACCGCCTCCGTCGACAAATCGATCAAGCTCTGGGATGTCAGGTCCATCCGGGCTCCAATTTCGGTCCTCAACGGCCACGGCTACGCCGTCAGAAAAGTCAAATTCTCGCCGCACCGGCAGAGCTTGATGATGTCGTGCTCGTACGACATGTCGGTTTGCCTTTGGGATTACATGGTGGAGGATGCGCTGGTGGGGCGGTACGACCACCACTCGGAGTTCGCCGTTGGAATCGATATGAGCGTTTTGGTGGAGGGACTTCTTGCCAGCACCGGTTGGGATGAGCTTGTCTATATTTGGCAGCACGGTACTGACCCCAGAGCGCCTTGA

Protein Analysis

321

Amino Acids

35.69

Weight (kDa)

5.3

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 38 - 137 1.7e-07 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 60 - 183 3.1e-11 CDC20/Fizzy WD40 domain
WDR55 PF24796 62 - 227 3.9e-09 WDR55
WD40_Prp19 PF24814 62 - 184 4.3e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 65 - 225 1.3e-17 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 66 - 155 1e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 74 - 181 2.8e-06 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 103 - 311 9.1e-12 G protein beta WD-40 repeat protein
EIF3I PF24805 104 - 212 7.5e-06 EIF3I
Beta-prop_THOC3 PF25174 108 - 312 1.5e-25 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 125 - 312 8.4e-29 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 128 - 282 1.7e-17 CDC20/Fizzy WD40 domain
Beta-prop_EIPR1 PF23609 154 - 267 6e-15 EIPR1 beta-propeller
Beta-prop_EML_2 PF23414 163 - 308 2.6e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 166 - 313 1.7e-10 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 170 - 273 4.3e-10 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd PF25172 194 - 316 2.1e-07 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 197 - 287 1.4e-09 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 207 - 313 1.4e-12 WDR3 first beta-propeller domain
WD40 PF00400 231 - 268 1e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29260
fragaria_vesca FvH4_3g07730
malus_domestica MD05G1300300.v1.1 MD10G1279400.v1.1
prunus_persica Prupe.4G062000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0010881
rosa_laevigata RLG00000031794
rosa_multiflora Rmu_sc0000109.1_g000002
rosa_roxburghii Rroxscaffold_1G00065480
rosa_rugosa Rorug04G0453500
rosa_samantha Rh5AG086200 Rh5BG080400 Rh5CG092900 Rh5DG080600
rosa_wichuraiana Rw0G017460 Rw0G017910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 260, 398, 626
AatII GACGTC 2 cut(s) 207, 501
AccBSI CCGCTC 1 cut(s) 309
AccI GTMKAC 4 cut(s) 198, 417, 465, 648
AccII CGCG 6 cut(s) 140, 328, 330, 361, 366, 534
AclWI GGATC 3 cut(s) 552, 565, 586
AcoI YGGCCR 2 cut(s) 109, 709
AcsI RAATTY 1 cut(s) 737
AcyI GRCGYC 3 cut(s) 195, 204, 498
AfaI GTAC 4 cut(s) 617, 779, 836, 943
AfiI CCNNNNNNNGG 4 cut(s) 26, 477, 606, 847
AflIII ACRYGT 2 cut(s) 532, 783
AgeI ACCGGT 1 cut(s) 905
AgsI TTSAA 3 cut(s) 13, 25, 445
AjiI CACGTC 2 cut(s) 121, 544
AjnI CCWGG 2 cut(s) 209, 386
AluBI AGCT 7 cut(s) 169, 268, 339, 406, 664, 759, 919
AluI AGCT 7 cut(s) 169, 268, 339, 406, 664, 759, 919
Alw21I GWGCWC 4 cut(s) 171, 324, 453, 776
Alw26I GTCTC 3 cut(s) 119, 178, 225
AlwI GGATC 3 cut(s) 552, 565, 586
AoxI GGCC 3 cut(s) 109, 141, 709
ApeKI GCWGC 1 cut(s) 934
ApoI RAATTY 1 cut(s) 737
AsiGI ACCGGT 1 cut(s) 905
AspLEI GCGC 5 cut(s) 330, 363, 580, 823, 958
AspS9I GGNCC 6 cut(s) 142, 148, 213, 411, 678, 700
AsuC2I CCSGG 4 cut(s) 146, 555, 575, 686
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 5 cut(s) 148, 213, 411, 678, 700
BamHI GGATCC 1 cut(s) 557
BanII GRGCYC 3 cut(s) 171, 555, 691
BauI CACGAG 2 cut(s) 317, 581
BbsI GAAGAC 1 cut(s) 493
Bbv12I GWGCWC 4 cut(s) 171, 324, 453, 776
BbvI GCAGC 1 cut(s) 946
BccI CCATC 2 cut(s) 254, 689
BciT130I CCWGG 2 cut(s) 211, 388
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 4 cut(s) 146, 555, 575, 686
BcoDI GTCTC 3 cut(s) 119, 178, 225
BfoI RGCGCY 1 cut(s) 959
BglI GCCNNNNNGGC 1 cut(s) 751
BisI GCNGC 5 cut(s) 141, 243, 364, 746, 935
BlsI GCNGC 5 cut(s) 142, 244, 365, 747, 936
Bme1390I CCNGG 6 cut(s) 146, 211, 388, 555, 575, 686
Bme18I GGWCC 5 cut(s) 148, 213, 411, 678, 700
BmgBI CACGTC 2 cut(s) 121, 544
BmgT120I GGNCC 6 cut(s) 142, 148, 213, 411, 678, 700
BmiI GGNNCC 6 cut(s) 144, 150, 259, 482, 559, 690
BmrFI CCNGG 6 cut(s) 146, 211, 388, 555, 575, 686
BmsI GCATC 2 cut(s) 105, 808
BpiI GAAGAC 1 cut(s) 493
BplI GAGNNNNNCTC 6 cut(s) 158, 190, 323, 355, 581, 613
BpuMI CCSGG 4 cut(s) 146, 555, 575, 686
Bsa29I ATCGAT 2 cut(s) 656, 867
BsaHI GRCGYC 3 cut(s) 195, 204, 498
BsaJI CCNNGG 5 cut(s) 100, 138, 144, 387, 712
BsaWI WCCGGW 1 cut(s) 905
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 4 cut(s) 26, 477, 606, 847
Bse118I RCCGGY 3 cut(s) 4, 750, 905
BseBI CCWGG 2 cut(s) 211, 388
BseCI ATCGAT 2 cut(s) 656, 867
BseDI CCNNGG 5 cut(s) 100, 138, 144, 387, 712
BseGI GGATG 6 cut(s) 96, 570, 676, 681, 823, 919
BseLI CCNNNNNNNGG 4 cut(s) 26, 477, 606, 847
BseRI GAGGAG 1 cut(s) 224
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 946
BsgI GTGCAG 1 cut(s) 508
Bsh1236I CGCG 6 cut(s) 140, 328, 330, 361, 366, 534
Bsh1285I CGRYCG 2 cut(s) 112, 421
BshFI GGCC 3 cut(s) 111, 143, 711
BshTI ACCGGT 1 cut(s) 905
BshVI ATCGAT 2 cut(s) 656, 867
BsiEI CGRYCG 2 cut(s) 112, 421
BsiHKAI GWGCWC 4 cut(s) 171, 324, 453, 776
BsiSI CCGG 8 cut(s) 5, 146, 516, 555, 575, 685, 751, 906
BsiWI CGTACG 1 cut(s) 777
BslFI GGGAC 5 cut(s) 46, 134, 404, 554, 903
BslI CCNNNNNNNGG 4 cut(s) 26, 477, 606, 847
BsmAI GTCTC 3 cut(s) 119, 178, 225
BsmBI CGTCTC 2 cut(s) 119, 178
BsmFI GGGAC 5 cut(s) 46, 134, 404, 554, 903
BsnI GGCC 3 cut(s) 111, 143, 711
Bsp1286I GDGCHC 6 cut(s) 171, 324, 453, 555, 691, 776
Bsp143I GATC 4 cut(s) 557, 567, 591, 657
BspANI GGCC 3 cut(s) 111, 143, 711
BspDI ATCGAT 2 cut(s) 656, 867
BspFNI CGCG 6 cut(s) 140, 328, 330, 361, 366, 534
BspLI GGNNCC 6 cut(s) 144, 150, 259, 482, 559, 690
BspPI GGATC 3 cut(s) 552, 565, 586
BsrBI CCGCTC 1 cut(s) 309
BsrFI RCCGGY 3 cut(s) 4, 750, 905
BssAI RCCGGY 3 cut(s) 4, 750, 905
BssECI CCNNGG 5 cut(s) 100, 138, 144, 387, 712
BssMI GATC 4 cut(s) 557, 567, 591, 657
BssNI GRCGYC 3 cut(s) 195, 204, 498
BssSI CACGAG 2 cut(s) 317, 581
Bst2BI CACGAG 2 cut(s) 317, 581
Bst2UI CCWGG 2 cut(s) 211, 388
Bst4CI ACNGT 3 cut(s) 400, 415, 941
BstACI GRCGYC 3 cut(s) 195, 204, 498
BstC8I GCNNGC 3 cut(s) 453, 532, 901
BstDSI CCRYGG 2 cut(s) 138, 712
BstF5I GGATG 6 cut(s) 96, 570, 676, 681, 823, 919
BstFNI CGCG 6 cut(s) 140, 328, 330, 361, 366, 534
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 5 cut(s) 330, 363, 580, 823, 958
BstKTI GATC 4 cut(s) 560, 570, 594, 660
BstMAI GTCTC 3 cut(s) 119, 178, 225
BstMBI GATC 4 cut(s) 557, 567, 591, 657
BstMCI CGRYCG 2 cut(s) 112, 421
BstMWI GCNNNNNNNGC 6 cut(s) 102, 248, 336, 531, 751, 829
BstNI CCWGG 2 cut(s) 211, 388
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 6 cut(s) 144, 209, 386, 553, 573, 684
BstUI CGCG 6 cut(s) 140, 328, 330, 361, 366, 534
BstV1I GCAGC 1 cut(s) 946
BstV2I GAAGAC 1 cut(s) 493
BstX2I RGATCY 2 cut(s) 557, 591
BstYI RGATCY 2 cut(s) 557, 591
BstZI CGGCCG 1 cut(s) 109
Bsu15I ATCGAT 2 cut(s) 656, 867
BsuRI GGCC 3 cut(s) 111, 143, 711
BsuTUI ATCGAT 2 cut(s) 656, 867
BtgI CCRYGG 2 cut(s) 138, 712
BtgZI GCGATG 2 cut(s) 232, 616
BtrI CACGTC 2 cut(s) 121, 544
BtsCI GGATG 6 cut(s) 96, 570, 676, 681, 823, 919
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 453, 532, 901
CfoI GCGC 5 cut(s) 330, 363, 580, 823, 958
Cfr10I RCCGGY 3 cut(s) 4, 750, 905
Cfr13I GGNCC 6 cut(s) 142, 148, 213, 411, 678, 700
Cfr42I CCGCGG 1 cut(s) 141
ClaI ATCGAT 2 cut(s) 656, 867
CseI GACGC 4 cut(s) 184, 451, 523, 616
Csp6I GTAC 4 cut(s) 616, 778, 835, 942
CspAI ACCGGT 1 cut(s) 905
CviAII CATG 3 cut(s) 565, 784, 808
CviQI GTAC 4 cut(s) 616, 778, 835, 942
DpnI GATC 4 cut(s) 559, 569, 593, 659
DpnII GATC 4 cut(s) 557, 567, 591, 657
DrdI GACNNNNNNGTC 3 cut(s) 260, 398, 626
DseDI GACNNNNNNGTC 3 cut(s) 260, 398, 626
EaeI YGGCCR 2 cut(s) 109, 709
EagI CGGCCG 1 cut(s) 109
EciI GGCGGA 3 cut(s) 331, 460, 499
Ecl136II GAGCTC 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 109
Eco24I GRGCYC 3 cut(s) 171, 555, 691
Eco47I GGWCC 5 cut(s) 148, 213, 411, 678, 700
Eco52I CGGCCG 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 209, 386
EcoT38I GRGCYC 3 cut(s) 171, 555, 691
Esp3I CGTCTC 2 cut(s) 119, 178
FaeI CATG 3 cut(s) 568, 787, 811
FaiI YATR 5 cut(s) 566, 785, 809, 872, 927
FaqI GGGAC 5 cut(s) 46, 134, 404, 554, 903
FatI CATG 3 cut(s) 564, 783, 807
FauI CCCGC 4 cut(s) 71, 145, 433, 494
FbaI TGATCA 1 cut(s) 567
FblI GTMKAC 4 cut(s) 198, 417, 465, 648
Fnu4HI GCNGC 5 cut(s) 141, 243, 364, 746, 935
FokI GGATG 6 cut(s) 83, 557, 668, 683, 830, 926
FriOI GRGCYC 3 cut(s) 171, 555, 691
Fsp4HI GCNGC 5 cut(s) 141, 243, 364, 746, 935
GlaI GCGC 5 cut(s) 329, 362, 579, 822, 957
GluI GCNGC 5 cut(s) 141, 243, 364, 746, 935
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 3 cut(s) 111, 143, 711
HapII CCGG 8 cut(s) 5, 146, 516, 555, 575, 685, 751, 906
HgaI GACGC 4 cut(s) 184, 451, 523, 616
HhaI GCGC 5 cut(s) 330, 363, 580, 823, 958
Hin1I GRCGYC 3 cut(s) 195, 204, 498
Hin1II CATG 3 cut(s) 568, 787, 811
Hin6I GCGC 5 cut(s) 328, 361, 578, 821, 956
HinP1I GCGC 5 cut(s) 328, 361, 578, 821, 956
HincII GTYRAC 2 cut(s) 418, 649
HindII GTYRAC 2 cut(s) 418, 649
HinfI GANTC 5 cut(s) 59, 218, 227, 368, 864
HpaII CCGG 8 cut(s) 5, 146, 516, 555, 575, 685, 751, 906
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 4 cut(s) 199, 418, 466, 649
Hpy188I TCNGA 3 cut(s) 217, 728, 850
Hpy188III TCNNGA 4 cut(s) 224, 409, 478, 589
Hpy8I GTNNAC 4 cut(s) 199, 418, 466, 649
Hpy99I CGWCG 9 cut(s) 77, 194, 206, 209, 257, 419, 500, 623, 650
HpyAV CCTTC 3 cut(s) 61, 187, 451
HpyCH4III ACNGT 3 cut(s) 400, 415, 941
HpyCH4IV ACGT 4 cut(s) 120, 204, 498, 543
HpyCH4V TGCA 1 cut(s) 525
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 248, 336, 531, 751, 829
HpySE526I ACGT 4 cut(s) 120, 204, 498, 543
Hsp92I GRCGYC 3 cut(s) 195, 204, 498
Hsp92II CATG 3 cut(s) 568, 787, 811
HspAI GCGC 5 cut(s) 328, 361, 578, 821, 956
Ksp22I TGATCA 1 cut(s) 567
KspI CCGCGG 1 cut(s) 141
Kzo9I GATC 4 cut(s) 557, 567, 591, 657
LmnI GCTCC 5 cut(s) 263, 314, 344, 448, 694
Lsp1109I GCAGC 1 cut(s) 946
LweI GCATC 2 cut(s) 105, 808
MaeII ACGT 4 cut(s) 120, 204, 498, 543
MaeIII GTNAC 1 cut(s) 160
MalI GATC 4 cut(s) 559, 569, 593, 659
MbiI CCGCTC 1 cut(s) 309
MboI GATC 4 cut(s) 557, 567, 591, 657
MboII GAAGA 1 cut(s) 493
MflI RGATCY 2 cut(s) 557, 591
MhlI GDGCHC 6 cut(s) 171, 324, 453, 555, 691, 776
MluCI AATT 2 cut(s) 693, 737
MluI ACGCGT 1 cut(s) 532
MlyI GAGTC 4 cut(s) 68, 221, 227, 362
MmeI TCCRAC 2 cut(s) 321, 841
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 8 cut(s) 5, 146, 516, 555, 575, 685, 751, 906
MspR9I CCNGG 6 cut(s) 146, 211, 388, 555, 575, 686
MvaI CCWGG 2 cut(s) 211, 388
MvnI CGCG 6 cut(s) 140, 328, 330, 361, 366, 534
MwoI GCNNNNNNNGC 6 cut(s) 102, 248, 336, 531, 751, 829
NciI CCSGG 4 cut(s) 146, 555, 575, 686
NdeII GATC 4 cut(s) 557, 567, 591, 657
NlaIII CATG 3 cut(s) 568, 787, 811
NlaIV GGNNCC 6 cut(s) 144, 150, 259, 482, 559, 690
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 160
NspI RCATGY 1 cut(s) 787
PciI ACATGT 1 cut(s) 783
PcsI WCGNNNNNNNCGW 9 cut(s) 33, 177, 186, 198, 213, 459, 624, 720, 873
PfeI GAWTC 1 cut(s) 864
Pfl23II CGTACG 1 cut(s) 777
PflFI GACNNNGTC 3 cut(s) 194, 398, 626
PinAI ACCGGT 1 cut(s) 905
PkrI GCNGC 5 cut(s) 142, 244, 365, 747, 936
PleI GAGTC 4 cut(s) 67, 221, 226, 362
PpsI GAGTC 4 cut(s) 67, 221, 226, 362
PscI ACATGT 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 209, 386
PspGI CCWGG 2 cut(s) 209, 386
PspLI CGTACG 1 cut(s) 777
PspN4I GGNNCC 6 cut(s) 144, 150, 259, 482, 559, 690
PspPI GGNCC 6 cut(s) 142, 148, 213, 411, 678, 700
PsuI RGATCY 2 cut(s) 557, 591
PsyI GACNNNGTC 3 cut(s) 194, 398, 626
RsaI GTAC 4 cut(s) 617, 779, 836, 943
RsaNI GTAC 4 cut(s) 616, 778, 835, 942
SacI GAGCTC 1 cut(s) 171
SacII CCGCGG 1 cut(s) 141
SalI GTCGAC 2 cut(s) 416, 647
SatI GCNGC 5 cut(s) 141, 243, 364, 746, 935
Sau3AI GATC 4 cut(s) 557, 567, 591, 657
Sau96I GGNCC 6 cut(s) 142, 148, 213, 411, 678, 700
SchI GAGTC 4 cut(s) 68, 221, 227, 362
ScrFI CCNGG 6 cut(s) 146, 211, 388, 555, 575, 686
SduI GDGCHC 6 cut(s) 171, 324, 453, 555, 691, 776
SfaNI GCATC 2 cut(s) 105, 808
Sfr303I CCGCGG 1 cut(s) 141
SgrBI CCGCGG 1 cut(s) 141
SinI GGWCC 5 cut(s) 148, 213, 411, 678, 700
Sse9I AATT 2 cut(s) 693, 737
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 6 cut(s) 144, 209, 386, 553, 573, 684
TaaI ACNGT 3 cut(s) 400, 415, 941
TaiI ACGT 4 cut(s) 123, 207, 501, 546
TaqI TCGA 7 cut(s) 126, 180, 417, 588, 648, 656, 867
TaqII GACCGA 1 cut(s) 688
TasI AATT 2 cut(s) 693, 737
TauI GCSGC 4 cut(s) 143, 245, 366, 748
TfiI GAWTC 1 cut(s) 864
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 934
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 4 cut(s) 25, 250, 421, 634
TspRI CASTG 1 cut(s) 385
Tth111I GACNNNGTC 3 cut(s) 194, 398, 626
VpaK11BI GGWCC 5 cut(s) 148, 213, 411, 678, 700
XapI RAATTY 1 cut(s) 737
XceI RCATGY 1 cut(s) 787
XmiI GTMKAC 4 cut(s) 198, 417, 465, 648
ZraI GACGTC 2 cut(s) 205, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.