RLG00000032178

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018618
Physical Location & Seq
Forward (+)
11836215 .. 11837912
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032178

Sequence Viewer

Length: 741 bp
ATGTCCTCACCTTCATCTCCAAAACGACCATGCGGAGCCCCGATCCGAAACTGGACGGAGCTCCCGGAGGACGTCACGGCTGCGATCCTCTCCCGTCTCGGAGCCATCGACATCCTCGAGACGGCTCAGAAGGTTTGCAAGACGTGGTACAAAGTCTGCAAGGACCCTCTTATGTGGCGCATCCTCGACTTCCGCAACGATGATCTCCCCAAAGTTCATGACTTGGGGAAGATGTGCCGCTACGCCGTTGATCGGAGCGATGGGAAATTGGCCAGTATCAATCTCGATTCCTTCGGCACCGATGAGCTCCTCAAGTACATCACTGATAGTCAATGGTACAGTTTCGCCCATGAGGAGTGTAACAAGCATGCACTTGCCATAGCAGGAACAATGCATAATTTATGCCACCTCCAGATATTTGCAAATAAGCTGACTAATGATGGCTTGCGGGCTATACTTGATGGTTGTCCTCCACTTGAGTCACTTGATCTGCGCCAGTGTTACAATCTTAATCTGGATGAAGATTTGGAGAAAAGATTGGTTGAATGTATTAAAATATTACGGCTTCCTGATGATTCAACTGATGACTATGAGTTTGATGCTACAGTTTTCGAGTACGGATCAGATGATGCAGAAAATGAAGTTGGAATTTTTGACGAAATGTTTATGGTAGATCATGAAGATGGTGACTTCCCCTGGAATGGTTTCGTTGAGTTTGATGATTTCTATTACTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

247

Amino Acids

28.24

Weight (kDa)

4.45

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 64 7.4e-10 F-box-like
F-box PF00646 18 - 60 6.2e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018618)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0015231
rosa_laevigata RLG00000032178 RLG00000032179
rosa_multiflora Rmu_sc0002209.1_g000023
rosa_roxburghii Rroxscaffold_1G00061340
rosa_rugosa Rorug05G0024800
rosa_samantha Rh5BG117400 Rh5CG128500 Rh5DG116500
rosa_wichuraiana Rw5G010310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 296
AciI CCGC 4 cut(s) 33, 193, 238, 448
AclWI GGATC 3 cut(s) 37, 79, 628
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 4 cut(s) 149, 317, 338, 617
AfiI CCNNNNNNNGG 3 cut(s) 121, 252, 701
AgsI TTSAA 2 cut(s) 545, 579
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 1 cut(s) 695
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AluBI AGCT 3 cut(s) 61, 307, 430
AluI AGCT 3 cut(s) 61, 307, 430
Alw21I GWGCWC 2 cut(s) 63, 309
Alw26I GTCTC 2 cut(s) 101, 113
AlwI GGATC 3 cut(s) 37, 79, 628
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 1 cut(s) 648
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
Asp700I GAANNNNTTC 1 cut(s) 704
AspLEI GCGC 2 cut(s) 180, 495
AspS9I GGNCC 1 cut(s) 163
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 698
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 163
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 3 cut(s) 40, 63, 309
Bbv12I GWGCWC 2 cut(s) 63, 309
BbvI GCAGC 1 cut(s) 67
BccI CCATC 5 cut(s) 113, 254, 434, 455, 677
BceAI ACGGC 4 cut(s) 93, 138, 230, 578
BciT130I CCWGG 1 cut(s) 697
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 101, 113
BfmI CTRYAG 1 cut(s) 603
BisI GCNGC 2 cut(s) 81, 238
BlsI GCNGC 2 cut(s) 82, 239
Bme1390I CCNGG 2 cut(s) 65, 697
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 4 cut(s) 37, 103, 165, 298
BmrFI CCNGG 2 cut(s) 65, 697
BmsI GCATC 3 cut(s) 189, 589, 619
BpmI CTGGAG 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 296, 497
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 695
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 252, 701
Bse1I ACTGG 3 cut(s) 56, 273, 496
BseBI CCWGG 1 cut(s) 697
BseDI CCNNGG 1 cut(s) 695
BseGI GGATG 3 cut(s) 111, 180, 523
BseLI CCNNNNNNNGG 3 cut(s) 121, 252, 701
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 3 cut(s) 56, 273, 496
BseRI GAGGAG 2 cut(s) 299, 368
BseXI GCAGC 1 cut(s) 67
BshFI GGCC 1 cut(s) 272
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 63, 309
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 3 cut(s) 121, 252, 701
BsmAI GTCTC 2 cut(s) 101, 113
BsmBI CGTCTC 2 cut(s) 101, 113
BsnI GGCC 1 cut(s) 272
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 40, 63, 309
Bsp143I GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
BspACI CCGC 4 cut(s) 33, 193, 238, 448
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 1 cut(s) 139
BspHI TCATGA 2 cut(s) 217, 676
BspLI GGNNCC 4 cut(s) 37, 103, 165, 298
BspPI GGATC 3 cut(s) 37, 79, 628
BspT107I GGYRCC 1 cut(s) 296
BsrI ACTGG 3 cut(s) 56, 273, 496
BssECI CCNNGG 1 cut(s) 695
BssMI GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 697
Bst4CI ACNGT 2 cut(s) 341, 607
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 3 cut(s) 369, 446, 450
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 3 cut(s) 111, 180, 523
BstHHI GCGC 2 cut(s) 180, 495
BstKTI GATC 7 cut(s) 45, 87, 205, 253, 490, 623, 676
BstMAI GTCTC 2 cut(s) 101, 113
BstMBI GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
BstNI CCWGG 1 cut(s) 697
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 63, 695
BstSFI CTRYAG 1 cut(s) 603
BstV1I GCAGC 1 cut(s) 67
BsuRI GGCC 1 cut(s) 272
BtgZI GCGATG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 3 cut(s) 111, 180, 523
BtsIMutI CAGTG 2 cut(s) 321, 503
Cac8I GCNNGC 3 cut(s) 369, 446, 450
CciI TCATGA 2 cut(s) 217, 676
CfoI GCGC 2 cut(s) 180, 495
Cfr13I GGNCC 1 cut(s) 163
Csp6I GTAC 4 cut(s) 148, 316, 337, 616
CviAII CATG 5 cut(s) 30, 218, 350, 368, 677
CviQI GTAC 4 cut(s) 148, 316, 337, 616
DdeI CTNAG 1 cut(s) 126
DpnI GATC 7 cut(s) 44, 86, 204, 252, 489, 622, 675
DpnII GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
EaeI YGGCCR 1 cut(s) 270
Ecl136II GAGCTC 2 cut(s) 61, 307
Eco24I GRGCYC 3 cut(s) 40, 63, 309
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 2 cut(s) 61, 307
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 2 cut(s) 61, 307
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 695
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 3 cut(s) 40, 63, 309
Esp3I CGTCTC 2 cut(s) 101, 113
FaeI CATG 5 cut(s) 33, 221, 353, 371, 680
FatI CATG 5 cut(s) 29, 217, 349, 367, 676
FauI CCCGC 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 81, 238
FokI GGATG 3 cut(s) 98, 167, 530
FriOI GRGCYC 3 cut(s) 40, 63, 309
Fsp4HI GCNGC 2 cut(s) 81, 238
GlaI GCGC 2 cut(s) 179, 494
GluI GCNGC 2 cut(s) 81, 238
GsuI CTGGAG 1 cut(s) 395
HaeIII GGCC 1 cut(s) 272
HapII CCGG 1 cut(s) 65
HhaI GCGC 2 cut(s) 180, 495
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 5 cut(s) 33, 221, 353, 371, 680
Hin6I GCGC 2 cut(s) 178, 493
HinP1I GCGC 2 cut(s) 178, 493
HinfI GANTC 3 cut(s) 287, 479, 575
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 698
Hpy188I TCNGA 5 cut(s) 47, 101, 129, 255, 625
Hpy188III TCNNGA 8 cut(s) 118, 218, 284, 412, 515, 569, 677, 738
HpyAV CCTTC 3 cut(s) 21, 124, 301
HpyCH4III ACNGT 2 cut(s) 341, 607
HpyCH4IV ACGT 2 cut(s) 72, 143
HpyCH4V TGCA 6 cut(s) 138, 159, 371, 394, 422, 632
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 2 cut(s) 72, 143
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 5 cut(s) 33, 221, 353, 371, 680
HspAI GCGC 2 cut(s) 178, 493
Kzo9I GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
LmnI GCTCC 6 cut(s) 35, 58, 66, 101, 255, 312
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 3 cut(s) 189, 589, 619
MaeII ACGT 2 cut(s) 72, 143
MaeIII GTNAC 5 cut(s) 73, 359, 480, 500, 686
MalI GATC 7 cut(s) 44, 86, 204, 252, 489, 622, 675
MboI GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
MboII GAAGA 3 cut(s) 241, 533, 692
MhlI GDGCHC 3 cut(s) 40, 63, 309
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 3 cut(s) 266, 397, 648
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 625
Mox20I TGGCCA 1 cut(s) 272
Mph1103I ATGCAT 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 704
MscI TGGCCA 1 cut(s) 272
MseI TTAA 2 cut(s) 510, 552
MslI CAYNNNNRTG 1 cut(s) 681
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 65, 697
MvaI CCWGG 1 cut(s) 697
NciI CCSGG 1 cut(s) 65
NdeII GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
NlaIII CATG 5 cut(s) 33, 221, 353, 371, 680
NlaIV GGNNCC 4 cut(s) 37, 103, 165, 298
NmuCI GTSAC 3 cut(s) 73, 480, 686
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 1 cut(s) 371
PaeI GCATGC 1 cut(s) 371
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 2 cut(s) 217, 676
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 114
PdmI GAANNNNTTC 1 cut(s) 704
PfeI GAWTC 2 cut(s) 287, 575
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 2 cut(s) 82, 239
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 163
Psp124BI GAGCTC 2 cut(s) 63, 309
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 695
PspGI CCWGG 1 cut(s) 695
PspN4I GGNNCC 4 cut(s) 37, 103, 165, 298
PspPI GGNCC 1 cut(s) 163
PspPPI RGGWCCY 1 cut(s) 163
RsaI GTAC 4 cut(s) 149, 317, 338, 617
RsaNI GTAC 4 cut(s) 148, 316, 337, 616
RseI CAYNNNNRTG 1 cut(s) 681
SacI GAGCTC 2 cut(s) 63, 309
SaqAI TTAA 2 cut(s) 510, 552
SatI GCNGC 2 cut(s) 81, 238
Sau3AI GATC 7 cut(s) 42, 84, 202, 250, 487, 620, 673
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 2 cut(s) 65, 697
SduI GDGCHC 3 cut(s) 40, 63, 309
SetI ASST 8 cut(s) 13, 63, 75, 135, 146, 309, 411, 432
SfaNI GCATC 3 cut(s) 189, 589, 619
SfcI CTRYAG 1 cut(s) 603
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 1 cut(s) 163
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 3 cut(s) 116, 311, 476
SmoI CTYRAG 3 cut(s) 116, 311, 476
SphI GCATGC 1 cut(s) 371
Sse9I AATT 3 cut(s) 266, 397, 648
SsiI CCGC 4 cut(s) 33, 193, 238, 448
SspI AATATT 1 cut(s) 558
SstI GAGCTC 2 cut(s) 63, 309
StyD4I CCNGG 2 cut(s) 63, 695
TaaI ACNGT 2 cut(s) 341, 607
TaiI ACGT 2 cut(s) 75, 146
TaqI TCGA 5 cut(s) 108, 117, 186, 285, 612
TasI AATT 3 cut(s) 266, 397, 648
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 2 cut(s) 287, 575
Tru1I TTAA 2 cut(s) 510, 552
Tru9I TTAA 2 cut(s) 510, 552
TscAI CASTG 2 cut(s) 328, 503
TseFI GTSAC 3 cut(s) 73, 480, 686
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 3 cut(s) 73, 480, 686
TspDTI ATGAA 4 cut(s) 206, 534, 654, 693
TspGWI ACGGA 2 cut(s) 71, 633
TspRI CASTG 2 cut(s) 328, 503
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 648
XceI RCATGY 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 704
ZraI GACGTC 1 cut(s) 73
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.