Rw5G010310

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018618
Physical Location & Seq
Forward (+)
11304949 .. 11306661
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010310.1

Sequence Viewer

Length: 927 bp
ATGTCCTCACCTTCATCTCCAAAACGACCACGCGGAGCCCCGATCCGAAACTGGACGGAGCTCCCGGAGGACTTCACGGCGGCGATCCTCTCCCGTCTCGGAGCCATCGACATCCTCGAGACGGCTCAGAAGGTCTGCAAGACGTGGTACAGAGTCTGCAAGGACCCTCTCATGTGGCGCACCGTCGACTTCCGCGACGATGATCTCCACAAAGTTCATGACTTGGGGAAGATGTGCCGCTACGCCGTTGATCGGAGCGATGGGAAATTGGCCAGTATCAATCTCGATTCCTTCGCCACCGATGAGCTCCTCAAGTACATCACTGATAGTTCAAGTGAAATCAGATGTCTACGACTTGTGCGTTGCAACATAACAAGTGAGGGATTGAGTGCAGTGTCTTCAAGACTTCCACTGTTGGAGGAACTTGAAATTTCACATTGCCCGCTCTCACATGAAACTCTAGAAGTGGTTGGGGCCTCTTGTCCTCTTTTGAAATCACTGAAACTCAACAGTCGATGGTACAGTTTCACCCATGAGGAGTGTAACAAGCATGCACTTGCCATAGCAGGAACAATGCATAATTTATGCCACCTCCAGATTTTTGCGAATAAGCTGACTAATGATGGCTTGCAGGCTATACTTGATGGTTGTCCTCCACTTGAGTCACTTGATCTGCGCCAGTGTTACAATCTTAATCTGGAGGAAAATTTTGAGAAAAGATTGGTGGAACGAATTAAACTTTTACGGCTTCCGGATGATTCAACTGATGACTATGAGTTTGATGCTACGGTTCTCGAGTACGGATCAGATAATCCAGAGCCTGGAGTCGAATTTTTTGACGAAATTTTCATGGTAGATGATGCAGATGGTGAAGTCTTCCCCTGGAATGGTTTCGTTGAGTTTGAGGATTTCTATTACTTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

308

Amino Acids

35.2

Weight (kDa)

4.74

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 65 6.1e-10 F-box-like
F-box PF00646 18 - 60 5.1e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018618)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0015231
rosa_laevigata RLG00000032178 RLG00000032179
rosa_multiflora Rmu_sc0002209.1_g000023
rosa_roxburghii Rroxscaffold_1G00061340
rosa_rugosa Rorug05G0024800
rosa_samantha Rh5BG117400 Rh5CG128500 Rh5DG116500
rosa_wichuraiana Rw5G010310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 821
AccBSI CCGCTC 1 cut(s) 445
AccI GTMKAC 2 cut(s) 186, 349
AccII CGCG 2 cut(s) 33, 195
AccIII TCCGGA 1 cut(s) 751
AciI CCGC 5 cut(s) 33, 80, 193, 238, 443
AclWI GGATC 3 cut(s) 37, 79, 811
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 4 cut(s) 429, 706, 830, 843
AfaI GTAC 4 cut(s) 149, 317, 521, 800
AfiI CCNNNNNNNGG 4 cut(s) 121, 252, 821, 887
AgsI TTSAA 5 cut(s) 333, 402, 428, 493, 762
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 2 cut(s) 820, 881
AluBI AGCT 3 cut(s) 61, 307, 613
AluI AGCT 3 cut(s) 61, 307, 613
Alw21I GWGCWC 2 cut(s) 63, 309
Alw26I GTCTC 2 cut(s) 101, 113
AlwI GGATC 3 cut(s) 37, 79, 811
AlwNI CAGNNNCTG 2 cut(s) 156, 821
Ama87I CYCGRG 2 cut(s) 116, 794
Aor13HI TCCGGA 1 cut(s) 751
AoxI GGCC 2 cut(s) 270, 474
ApoI RAATTY 4 cut(s) 429, 706, 830, 843
ArsI GACNNNNNNTTYG 2 cut(s) 761, 793
Asp700I GAANNNNTTC 1 cut(s) 890
AspLEI GCGC 2 cut(s) 180, 678
AspS9I GGNCC 2 cut(s) 163, 474
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 2 cut(s) 520, 881
AvaI CYCGRG 2 cut(s) 116, 794
AvaII GGWCC 1 cut(s) 163
BalI TGGCCA 1 cut(s) 272
BanII GRGCYC 3 cut(s) 40, 63, 309
BbsI GAAGAC 2 cut(s) 390, 868
Bbv12I GWGCWC 2 cut(s) 63, 309
BccI CCATC 6 cut(s) 113, 254, 510, 617, 638, 860
BceAI ACGGC 4 cut(s) 93, 138, 230, 761
BciT130I CCWGG 2 cut(s) 822, 883
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 101, 113
BfaI CTAG 1 cut(s) 461
BisI GCNGC 2 cut(s) 81, 238
BlsI GCNGC 2 cut(s) 82, 239
Bme1390I CCNGG 3 cut(s) 65, 822, 883
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 2 cut(s) 116, 794
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 163, 474
BmiI GGNNCC 4 cut(s) 37, 103, 165, 475
BmrFI CCNGG 3 cut(s) 65, 822, 883
BmsI GCATC 2 cut(s) 772, 850
BpiI GAAGAC 2 cut(s) 390, 868
BpmI CTGGAG 3 cut(s) 578, 719, 843
BpuEI CTTGAG 2 cut(s) 296, 680
BpuMI CCSGG 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 881
BsaWI WCCGGW 1 cut(s) 751
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 252, 821, 887
Bse1I ACTGG 3 cut(s) 56, 273, 679
Bse3DI GCAATG 1 cut(s) 436
BseAI TCCGGA 1 cut(s) 751
BseBI CCWGG 2 cut(s) 822, 883
BseDI CCNNGG 1 cut(s) 881
BseGI GGATG 2 cut(s) 111, 760
BseLI CCNNNNNNNGG 4 cut(s) 121, 252, 821, 887
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 3 cut(s) 56, 273, 679
BseRI GAGGAG 2 cut(s) 299, 551
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 2 cut(s) 33, 195
BshFI GGCC 2 cut(s) 272, 476
BsiHKAI GWGCWC 2 cut(s) 63, 309
BsiHKCI CYCGRG 2 cut(s) 116, 794
BsiSI CCGG 2 cut(s) 65, 752
BslI CCNNNNNNNGG 4 cut(s) 121, 252, 821, 887
BsmAI GTCTC 2 cut(s) 101, 113
BsmBI CGTCTC 2 cut(s) 101, 113
BsnI GGCC 2 cut(s) 272, 476
BsoBI CYCGRG 2 cut(s) 116, 794
Bsp1286I GDGCHC 3 cut(s) 40, 63, 309
Bsp13I TCCGGA 1 cut(s) 751
Bsp143I GATC 6 cut(s) 42, 84, 202, 250, 670, 803
BspACI CCGC 5 cut(s) 33, 80, 193, 238, 443
BspANI GGCC 2 cut(s) 272, 476
BspCNI CTCAG 1 cut(s) 139
BspEI TCCGGA 1 cut(s) 751
BspFNI CGCG 2 cut(s) 33, 195
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 4 cut(s) 37, 103, 165, 475
BspPI GGATC 3 cut(s) 37, 79, 811
BsrBI CCGCTC 1 cut(s) 445
BsrDI GCAATG 1 cut(s) 436
BsrI ACTGG 3 cut(s) 56, 273, 679
BssECI CCNNGG 1 cut(s) 881
BssMI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
Bst2UI CCWGG 2 cut(s) 822, 883
Bst4CI ACNGT 5 cut(s) 184, 414, 512, 524, 790
BstC8I GCNNGC 4 cut(s) 443, 552, 629, 633
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 111, 760
BstFNI CGCG 2 cut(s) 33, 195
BstHHI GCGC 2 cut(s) 180, 678
BstKTI GATC 6 cut(s) 45, 87, 205, 253, 673, 806
BstMAI GTCTC 2 cut(s) 101, 113
BstMBI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
BstNI CCWGG 2 cut(s) 822, 883
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 3 cut(s) 63, 820, 881
BstUI CGCG 2 cut(s) 33, 195
BstV2I GAAGAC 2 cut(s) 390, 868
BsuRI GGCC 2 cut(s) 272, 476
BtgZI GCGATG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 2 cut(s) 111, 760
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 5 cut(s) 321, 399, 410, 497, 686
Cac8I GCNNGC 4 cut(s) 443, 552, 629, 633
CaiI CAGNNNCTG 2 cut(s) 156, 821
CciI TCATGA 1 cut(s) 217
CfoI GCGC 2 cut(s) 180, 678
Cfr13I GGNCC 2 cut(s) 163, 474
Csp6I GTAC 4 cut(s) 148, 316, 520, 799
CviAII CATG 6 cut(s) 172, 218, 452, 533, 551, 850
CviQI GTAC 4 cut(s) 148, 316, 520, 799
DdeI CTNAG 1 cut(s) 126
DpnI GATC 6 cut(s) 44, 86, 204, 252, 672, 805
DpnII GATC 6 cut(s) 42, 84, 202, 250, 670, 803
EaeI YGGCCR 1 cut(s) 270
Ecl136II GAGCTC 2 cut(s) 61, 307
Eco24I GRGCYC 3 cut(s) 40, 63, 309
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 2 cut(s) 61, 307
Eco88I CYCGRG 2 cut(s) 116, 794
EcoICRI GAGCTC 2 cut(s) 61, 307
EcoO109I RGGNCCY 2 cut(s) 163, 474
EcoRII CCWGG 2 cut(s) 820, 881
EcoT22I ATGCAT 1 cut(s) 579
EcoT38I GRGCYC 3 cut(s) 40, 63, 309
Esp3I CGTCTC 2 cut(s) 101, 113
FaeI CATG 6 cut(s) 175, 221, 455, 536, 554, 853
FatI CATG 6 cut(s) 171, 217, 451, 532, 550, 849
FauI CCCGC 1 cut(s) 450
FblI GTMKAC 2 cut(s) 186, 349
Fnu4HI GCNGC 2 cut(s) 81, 238
FokI GGATG 2 cut(s) 98, 767
FriOI GRGCYC 3 cut(s) 40, 63, 309
Fsp4HI GCNGC 2 cut(s) 81, 238
FspBI CTAG 1 cut(s) 461
GlaI GCGC 2 cut(s) 179, 677
GluI GCNGC 2 cut(s) 81, 238
GsuI CTGGAG 3 cut(s) 578, 719, 843
HaeIII GGCC 2 cut(s) 272, 476
HapII CCGG 2 cut(s) 65, 752
HhaI GCGC 2 cut(s) 180, 678
Hin1II CATG 6 cut(s) 175, 221, 455, 536, 554, 853
Hin6I GCGC 2 cut(s) 178, 676
HinP1I GCGC 2 cut(s) 178, 676
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 5 cut(s) 153, 287, 662, 758, 825
HpaII CCGG 2 cut(s) 65, 752
HphI GGTGA 2 cut(s) 520, 881
Hpy166II GTNNAC 2 cut(s) 187, 350
Hpy188I TCNGA 6 cut(s) 47, 101, 129, 255, 344, 808
Hpy8I GTNNAC 2 cut(s) 187, 350
Hpy99I CGWCG 2 cut(s) 188, 200
HpyAV CCTTC 3 cut(s) 21, 124, 301
HpyCH4III ACNGT 5 cut(s) 184, 414, 512, 524, 790
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 8 cut(s) 138, 159, 366, 392, 554, 577, 631, 863
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 6 cut(s) 175, 221, 455, 536, 554, 853
HspAI GCGC 2 cut(s) 178, 676
Kpn2I TCCGGA 1 cut(s) 751
Kzo9I GATC 6 cut(s) 42, 84, 202, 250, 670, 803
LmnI GCTCC 6 cut(s) 35, 58, 66, 101, 255, 312
LweI GCATC 2 cut(s) 772, 850
MaeI CTAG 1 cut(s) 461
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 3 cut(s) 542, 663, 683
MalI GATC 6 cut(s) 44, 86, 204, 252, 672, 805
MbiI CCGCTC 1 cut(s) 445
MboI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
MboII GAAGA 3 cut(s) 241, 390, 868
MhlI GDGCHC 3 cut(s) 40, 63, 309
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 7 cut(s) 266, 429, 580, 706, 732, 830, 843
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 3 cut(s) 162, 671, 834
MmeI TCCRAC 1 cut(s) 396
Mox20I TGGCCA 1 cut(s) 272
Mph1103I ATGCAT 1 cut(s) 579
MroI TCCGGA 1 cut(s) 751
MroXI GAANNNNTTC 1 cut(s) 890
MscI TGGCCA 1 cut(s) 272
MseI TTAA 2 cut(s) 693, 735
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 2 cut(s) 65, 752
MspR9I CCNGG 3 cut(s) 65, 822, 883
MvaI CCWGG 2 cut(s) 822, 883
MvnI CGCG 2 cut(s) 33, 195
NciI CCSGG 1 cut(s) 65
NdeII GATC 6 cut(s) 42, 84, 202, 250, 670, 803
NlaIII CATG 6 cut(s) 175, 221, 455, 536, 554, 853
NlaIV GGNNCC 4 cut(s) 37, 103, 165, 475
NmuCI GTSAC 1 cut(s) 663
NsiI ATGCAT 1 cut(s) 579
NspI RCATGY 1 cut(s) 554
PaeI GCATGC 1 cut(s) 554
PaeR7I CTCGAG 2 cut(s) 116, 794
PagI TCATGA 1 cut(s) 217
PcsI WCGNNNNNNNCGW 4 cut(s) 105, 114, 192, 358
PdmI GAANNNNTTC 1 cut(s) 890
PfeI GAWTC 2 cut(s) 287, 758
PflMI CCANNNNNTGG 1 cut(s) 821
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 2 cut(s) 82, 239
PleI GAGTC 3 cut(s) 161, 670, 833
PpsI GAGTC 3 cut(s) 161, 670, 833
PpuMI RGGWCCY 1 cut(s) 163
Psp124BI GAGCTC 2 cut(s) 63, 309
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 2 cut(s) 820, 881
PspGI CCWGG 2 cut(s) 820, 881
PspN4I GGNNCC 4 cut(s) 37, 103, 165, 475
PspPI GGNCC 2 cut(s) 163, 474
PspPPI RGGWCCY 1 cut(s) 163
PstNI CAGNNNCTG 2 cut(s) 156, 821
RsaI GTAC 4 cut(s) 149, 317, 521, 800
RsaNI GTAC 4 cut(s) 148, 316, 520, 799
SacI GAGCTC 2 cut(s) 63, 309
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 693, 735
SatI GCNGC 2 cut(s) 81, 238
Sau3AI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
Sau96I GGNCC 2 cut(s) 163, 474
SchI GAGTC 3 cut(s) 162, 671, 834
ScrFI CCNGG 3 cut(s) 65, 822, 883
SduI GDGCHC 3 cut(s) 40, 63, 309
SetI ASST 7 cut(s) 13, 63, 135, 146, 309, 594, 615
SfaNI GCATC 2 cut(s) 772, 850
Sfr274I CTCGAG 2 cut(s) 116, 794
SinI GGWCC 1 cut(s) 163
SlaI CTCGAG 2 cut(s) 116, 794
SmlI CTYRAG 4 cut(s) 116, 311, 659, 794
SmoI CTYRAG 4 cut(s) 116, 311, 659, 794
SphI GCATGC 1 cut(s) 554
Sse9I AATT 7 cut(s) 266, 429, 580, 706, 732, 830, 843
SsiI CCGC 5 cut(s) 33, 80, 193, 238, 443
SspMI CTAG 1 cut(s) 461
SstI GAGCTC 2 cut(s) 63, 309
StyD4I CCNGG 3 cut(s) 63, 820, 881
TaaI ACNGT 5 cut(s) 184, 414, 512, 524, 790
TaiI ACGT 1 cut(s) 146
TaqI TCGA 7 cut(s) 108, 117, 186, 285, 514, 795, 828
TasI AATT 7 cut(s) 266, 429, 580, 706, 732, 830, 843
TatI WGTACW 1 cut(s) 315
TauI GCSGC 2 cut(s) 83, 240
TfiI GAWTC 2 cut(s) 287, 758
Tru1I TTAA 2 cut(s) 693, 735
Tru9I TTAA 2 cut(s) 693, 735
TscAI CASTG 5 cut(s) 328, 399, 417, 504, 686
TseFI GTSAC 1 cut(s) 663
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 3 cut(s) 206, 468, 838
TspGWI ACGGA 2 cut(s) 71, 816
TspRI CASTG 5 cut(s) 328, 399, 417, 504, 686
Van91I CCANNNNNTGG 1 cut(s) 821
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 4 cut(s) 429, 706, 830, 843
XbaI TCTAGA 1 cut(s) 460
XceI RCATGY 1 cut(s) 554
XhoI CTCGAG 2 cut(s) 116, 794
XmiI GTMKAC 2 cut(s) 186, 349
XmnI GAANNNNTTC 1 cut(s) 890
XspI CTAG 1 cut(s) 461
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.