RLG00000032179

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018618
Physical Location & Seq
Forward (+)
11838629 .. 11840853
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032179

Sequence Viewer

Length: 837 bp
ATGTCCTCATCTTCATCTCCAAAACGACCATGCGGAGCCCGGATCGGAAACTGGACGGAGCTCCCGGAGGACGTCACGGCGGCGATCCTCTCCCGTCTCGGAGCCATCGACATCCTCGAGACGGCTCAGAAGGTCTGCAAGACGTGGTACAAAGTCTGCAAGGACCCTCTCATGTGGCGCATCGTCGACTTTCGCGACGATGATCTCCCCAAAGTTCATGACTTGGGGAAGATGTGCCGCTACGCCGTTGATCGGAGCGATGGGAAATTGGCCAGTATCAATCTCGATTCCTTCGCCACCGATGAGCTCCTCAAGTACATCACTGATAGTCGATGGTACAGTTTCGCCCATGAGGAGTGTAACAAGCATGCACTTGCCATAGCAGGAACAATGCATAATTTACGCGACCTCCAGATTTTTGCGAATAAGCTGACTAATGATGGCTTGCAGGCTATACTTGATGGTTGTCCTCCACTTGAGTCACTTGATCTGCGTCAGTGTTACAATCTTTATCTGGAGGAAGATTTTGAGAAAAGATTAGTGGAACGAATTAAACTATTACGGCTTCCTGATGATTCAACTGATGACTATGAGTTTGATGCTACGTTTCTCGAGTACGGATCAGATAATCCAGAGCCTGAAGTTGAATTTTTTGATGAAATGTTCATGATAGATGATGCAGATGGTGAACTCTTCCCCTGGAATGGTTTCGTTGAAGTGTTAGTAATTGATCAAGAAGCTGCCTTAACTGAAGGAGCATTCCGTTGTCGCGGATCTGTTCAAAGATGGGCTGCTGCACCAGACCTTTACTTCTTCGACTCATCAGGTGCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

279

Amino Acids

31.66

Weight (kDa)

4.46

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 64 9.4e-10 F-box-like
F-box PF00646 18 - 60 7.5e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018618)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0015231
rosa_laevigata RLG00000032178 RLG00000032179
rosa_multiflora Rmu_sc0002209.1_g000023
rosa_roxburghii Rroxscaffold_1G00061340
rosa_rugosa Rorug05G0024800
rosa_samantha Rh5BG117400 Rh5CG128500 Rh5DG116500
rosa_wichuraiana Rw5G010310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 186
AccII CGCG 3 cut(s) 195, 405, 771
AciI CCGC 4 cut(s) 33, 80, 238, 771
AclWI GGATC 4 cut(s) 50, 79, 628, 781
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 1 cut(s) 647
AcuI CTGAAG 2 cut(s) 660, 771
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 4 cut(s) 149, 317, 338, 617
AfiI CCNNNNNNNGG 3 cut(s) 121, 252, 704
AgsI TTSAA 4 cut(s) 579, 647, 716, 782
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 1 cut(s) 698
AluBI AGCT 4 cut(s) 61, 307, 430, 740
AluI AGCT 4 cut(s) 61, 307, 430, 740
Alw21I GWGCWC 2 cut(s) 63, 309
Alw26I GTCTC 2 cut(s) 101, 113
AlwI GGATC 4 cut(s) 50, 79, 628, 781
AlwNI CAGNNNCTG 1 cut(s) 638
Ama87I CYCGRG 2 cut(s) 116, 611
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 3 cut(s) 740, 791, 794
ApoI RAATTY 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
Asp700I GAANNNNTTC 1 cut(s) 707
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 163
AsuC2I CCSGG 2 cut(s) 40, 65
AsuHPI GGTGA 1 cut(s) 698
AvaI CYCGRG 2 cut(s) 116, 611
AvaII GGWCC 1 cut(s) 163
BalI TGGCCA 1 cut(s) 272
BanII GRGCYC 3 cut(s) 40, 63, 309
Bbv12I GWGCWC 2 cut(s) 63, 309
BbvI GCAGC 3 cut(s) 727, 778, 781
BccI CCATC 7 cut(s) 113, 254, 327, 434, 455, 677, 780
BceAI ACGGC 4 cut(s) 93, 138, 230, 578
BciT130I CCWGG 1 cut(s) 700
BclI TGATCA 1 cut(s) 730
BcnI CCSGG 2 cut(s) 40, 65
BcoDI GTCTC 2 cut(s) 101, 113
BisI GCNGC 5 cut(s) 81, 238, 741, 792, 795
BlsI GCNGC 5 cut(s) 82, 239, 742, 793, 796
Bme1390I CCNGG 3 cut(s) 40, 65, 700
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 2 cut(s) 116, 611
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 3 cut(s) 37, 103, 165
BmrFI CCNGG 3 cut(s) 40, 65, 700
BmsI GCATC 3 cut(s) 189, 589, 667
BpmI CTGGAG 2 cut(s) 395, 536
BpuEI CTTGAG 2 cut(s) 296, 497
BpuMI CCSGG 2 cut(s) 40, 65
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 698
BsaXI ACNNNNNCTCC 2 cut(s) 393, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 252, 704
Bse1I ACTGG 2 cut(s) 56, 273
BseBI CCWGG 1 cut(s) 700
BseDI CCNNGG 1 cut(s) 698
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 3 cut(s) 121, 252, 704
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 56, 273
BseRI GAGGAG 2 cut(s) 299, 368
BseXI GCAGC 3 cut(s) 727, 778, 781
BsgI GTGCAG 1 cut(s) 780
Bsh1236I CGCG 3 cut(s) 195, 405, 771
BshFI GGCC 1 cut(s) 272
BsiHKAI GWGCWC 2 cut(s) 63, 309
BsiHKCI CYCGRG 2 cut(s) 116, 611
BsiSI CCGG 2 cut(s) 40, 65
BslI CCNNNNNNNGG 3 cut(s) 121, 252, 704
BsmAI GTCTC 2 cut(s) 101, 113
BsmBI CGTCTC 2 cut(s) 101, 113
BsmI GAATGC 1 cut(s) 758
BsnI GGCC 1 cut(s) 272
BsoBI CYCGRG 2 cut(s) 116, 611
Bsp1286I GDGCHC 3 cut(s) 40, 63, 309
Bsp143I GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
Bsp68I TCGCGA 1 cut(s) 195
BspACI CCGC 4 cut(s) 33, 80, 238, 771
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 1 cut(s) 139
BspFNI CGCG 3 cut(s) 195, 405, 771
BspHI TCATGA 2 cut(s) 217, 666
BspLI GGNNCC 3 cut(s) 37, 103, 165
BspPI GGATC 4 cut(s) 50, 79, 628, 781
BsrI ACTGG 2 cut(s) 56, 273
BssECI CCNNGG 1 cut(s) 698
BssMI GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 700
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 1 cut(s) 698
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 3 cut(s) 369, 446, 450
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 3 cut(s) 195, 405, 771
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 8 cut(s) 45, 87, 205, 253, 490, 623, 733, 776
BstMAI GTCTC 2 cut(s) 101, 113
BstMBI GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
BstNI CCWGG 1 cut(s) 700
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 3 cut(s) 38, 63, 698
BstUI CGCG 3 cut(s) 195, 405, 771
BstV1I GCAGC 3 cut(s) 727, 778, 781
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
BsuRI GGCC 1 cut(s) 272
BtgZI GCGATG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 321, 503
BtuMI TCGCGA 1 cut(s) 195
Cac8I GCNNGC 3 cut(s) 369, 446, 450
CaiI CAGNNNCTG 1 cut(s) 638
CciI TCATGA 2 cut(s) 217, 666
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 1 cut(s) 163
CseI GACGC 1 cut(s) 482
Csp6I GTAC 4 cut(s) 148, 316, 337, 616
CviAII CATG 6 cut(s) 30, 172, 218, 350, 368, 667
CviQI GTAC 4 cut(s) 148, 316, 337, 616
DdeI CTNAG 1 cut(s) 126
DpnI GATC 8 cut(s) 44, 86, 204, 252, 489, 622, 732, 775
DpnII GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 698
Ecl136II GAGCTC 2 cut(s) 61, 307
Eco24I GRGCYC 3 cut(s) 40, 63, 309
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 2 cut(s) 61, 307
Eco57I CTGAAG 2 cut(s) 660, 771
Eco88I CYCGRG 2 cut(s) 116, 611
EcoICRI GAGCTC 2 cut(s) 61, 307
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 698
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 3 cut(s) 40, 63, 309
Esp3I CGTCTC 2 cut(s) 101, 113
FaeI CATG 6 cut(s) 33, 175, 221, 353, 371, 670
FatI CATG 6 cut(s) 29, 171, 217, 349, 367, 666
FbaI TGATCA 1 cut(s) 730
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 81, 238, 741, 792, 795
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 3 cut(s) 40, 63, 309
Fsp4HI GCNGC 5 cut(s) 81, 238, 741, 792, 795
GlaI GCGC 1 cut(s) 179
GluI GCNGC 5 cut(s) 81, 238, 741, 792, 795
GsuI CTGGAG 2 cut(s) 395, 536
HaeIII GGCC 1 cut(s) 272
HapII CCGG 2 cut(s) 40, 65
HgaI GACGC 1 cut(s) 482
HhaI GCGC 1 cut(s) 180
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 6 cut(s) 33, 175, 221, 353, 371, 670
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 4 cut(s) 287, 479, 575, 818
HpaII CCGG 2 cut(s) 40, 65
HphI GGTGA 1 cut(s) 698
Hpy166II GTNNAC 2 cut(s) 187, 689
Hpy188I TCNGA 5 cut(s) 47, 101, 129, 255, 625
Hpy8I GTNNAC 2 cut(s) 187, 689
Hpy99I CGWCG 2 cut(s) 188, 200
HpyAV CCTTC 3 cut(s) 124, 301, 746
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 3 cut(s) 72, 143, 605
HpyCH4V TGCA 8 cut(s) 138, 159, 371, 394, 448, 680, 797, 830
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 3 cut(s) 72, 143, 605
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 6 cut(s) 33, 175, 221, 353, 371, 670
HspAI GCGC 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
LmnI GCTCC 7 cut(s) 35, 58, 66, 101, 255, 312, 755
Lsp1109I GCAGC 3 cut(s) 727, 778, 781
LweI GCATC 3 cut(s) 189, 589, 667
MaeII ACGT 3 cut(s) 72, 143, 605
MaeIII GTNAC 4 cut(s) 73, 359, 480, 500
MalI GATC 8 cut(s) 44, 86, 204, 252, 489, 622, 732, 775
MboI GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
MboII GAAGA 5 cut(s) 3, 241, 533, 685, 805
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 3 cut(s) 40, 63, 309
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 5 cut(s) 266, 397, 549, 647, 726
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 2 cut(s) 488, 812
Mox20I TGGCCA 1 cut(s) 272
Mph1103I ATGCAT 1 cut(s) 396
MroXI GAANNNNTTC 1 cut(s) 707
MscI TGGCCA 1 cut(s) 272
MseI TTAA 2 cut(s) 552, 746
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 2 cut(s) 40, 65
MspR9I CCNGG 3 cut(s) 40, 65, 700
Mva1269I GAATGC 1 cut(s) 758
MvaI CCWGG 1 cut(s) 700
MvnI CGCG 3 cut(s) 195, 405, 771
NciI CCSGG 2 cut(s) 40, 65
NdeII GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
NlaIII CATG 6 cut(s) 33, 175, 221, 353, 371, 670
NlaIV GGNNCC 3 cut(s) 37, 103, 165
NmuCI GTSAC 2 cut(s) 73, 480
NruI TCGCGA 1 cut(s) 195
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 1 cut(s) 371
PaeI GCATGC 1 cut(s) 371
PaeR7I CTCGAG 2 cut(s) 116, 611
PagI TCATGA 2 cut(s) 217, 666
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 114, 192
PctI GAATGC 1 cut(s) 758
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 2 cut(s) 287, 575
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 5 cut(s) 82, 239, 742, 793, 796
PleI GAGTC 2 cut(s) 487, 812
PpsI GAGTC 2 cut(s) 487, 812
PpuMI RGGWCCY 1 cut(s) 163
Psp124BI GAGCTC 2 cut(s) 63, 309
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 698
PspGI CCWGG 1 cut(s) 698
PspN4I GGNNCC 3 cut(s) 37, 103, 165
PspPI GGNCC 1 cut(s) 163
PspPPI RGGWCCY 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 773
RruI TCGCGA 1 cut(s) 195
RsaI GTAC 4 cut(s) 149, 317, 338, 617
RsaNI GTAC 4 cut(s) 148, 316, 337, 616
SacI GAGCTC 2 cut(s) 63, 309
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 2 cut(s) 552, 746
SatI GCNGC 5 cut(s) 81, 238, 741, 792, 795
Sau3AI GATC 8 cut(s) 42, 84, 202, 250, 487, 620, 730, 773
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 2 cut(s) 488, 812
ScrFI CCNGG 3 cut(s) 40, 65, 700
SduI GDGCHC 3 cut(s) 40, 63, 309
SfaNI GCATC 3 cut(s) 189, 589, 667
Sfr274I CTCGAG 2 cut(s) 116, 611
SinI GGWCC 1 cut(s) 163
SlaI CTCGAG 2 cut(s) 116, 611
SmlI CTYRAG 4 cut(s) 116, 311, 476, 611
SmoI CTYRAG 4 cut(s) 116, 311, 476, 611
SphI GCATGC 1 cut(s) 371
Sse9I AATT 5 cut(s) 266, 397, 549, 647, 726
SsiI CCGC 4 cut(s) 33, 80, 238, 771
SstI GAGCTC 2 cut(s) 63, 309
StyD4I CCNGG 3 cut(s) 38, 63, 698
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 3 cut(s) 75, 146, 608
TaqI TCGA 7 cut(s) 108, 117, 186, 285, 331, 612, 816
TasI AATT 5 cut(s) 266, 397, 549, 647, 726
TatI WGTACW 1 cut(s) 315
TauI GCSGC 2 cut(s) 83, 240
TfiI GAWTC 2 cut(s) 287, 575
Tru1I TTAA 2 cut(s) 552, 746
Tru9I TTAA 2 cut(s) 552, 746
TscAI CASTG 2 cut(s) 328, 503
TseFI GTSAC 2 cut(s) 73, 480
TseI GCWGC 3 cut(s) 740, 791, 794
Tsp45I GTSAC 2 cut(s) 73, 480
TspDTI ATGAA 3 cut(s) 206, 655, 672
TspGWI ACGGA 3 cut(s) 71, 633, 752
TspRI CASTG 2 cut(s) 328, 503
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 647
XceI RCATGY 1 cut(s) 371
XhoI CTCGAG 2 cut(s) 116, 611
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 707
ZraI GACGTC 1 cut(s) 73
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.