Rroxscaffold_1G00061340

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018618
Physical Location & Seq
Reverse (-)
83495462 .. 83497323
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061340.1

Sequence Viewer

Length: 927 bp
ATGTCCTCACCTTCATCTCCAAAACGACCATGCGGAGCCCCGATCCGAAACTGGACGGAGCTCCCGGAGGACGTCACGGCTGCGATCCTCTCCCGTCTCGGAGCCATCGACATCCTCGAGACGGCTCAGAAGGTCTGCAAGACGTGGTACAAAGTCTGCAAGGACCCTCTCATGTGGCGCATCGTCGACTTCCGCGACGATGATCACCACAAAGTTCATGACTTGGGGAAGATGTGCCGCTACGCCGTTGATCGGAGCGATGGGAAGTTGGCTAGTATCAATCTCGATTCCTTCGCCACCGATGACCTCCTCAACTACATCACTGATAGTTCAAGTGAAATCAGATGTCTACGACTTGTGCGTTGCAACATAACAAGTGAGGGATTGAGTGCAGTGTCTTCAAGACTTCCACTGTTGGAGGAACTTGAAATTTCACATTGCTCGCTCTCACATGAAACTCTAGAAGTGGTTGGGGCCTCTTGTCCTCTTTTGAAATCACTGAAACTCAACAGTCGATGGTACCGTTTTGCCCATGAGGAGTGTAACAAGCATGCACTTGCCATAGCAGGAACAATGCATAATTTACGCCACCTCCAGATTTTTGCGAATAAGCTGACTAATGATGGCTTGCAGGCTATACTTGATGGTTGTCCTCCACTTGAGTCACTTGATCTGCGCCAGTGTTACAATCTTTATCTGGAGGAAGATTTTCAGAAAAGATTGGTGGAACGAATTAAACTATTACGGCTTCCTGATGATTCAATTGTTGACTATGAGTTTGATGCTACGTTTCTCGAGTACGGATCAGATAATCCAGAGCCTGAAGTCGAATTTTTTGACGAAATGTTCATGGTAGGTGATGCAGATGGTGAAGTCTTCCCCTGGAATGGTTTCGTTGAGTTTGAGGATTTCTATTACTTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

308

Amino Acids

35.29

Weight (kDa)

4.81

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 64 1.1e-09 F-box-like
F-box PF00646 18 - 60 8.7e-07 F-box domain
LRR_At5g56370 PF24758 110 - 178 3.3e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018618)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0015231
rosa_laevigata RLG00000032178 RLG00000032179
rosa_multiflora Rmu_sc0002209.1_g000023
rosa_roxburghii Rroxscaffold_1G00061340
rosa_rugosa Rorug05G0024800
rosa_samantha Rh5BG117400 Rh5CG128500 Rh5DG116500
rosa_wichuraiana Rw5G010310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
Acc65I GGTACC 1 cut(s) 519
AccB1I GGYRCC 1 cut(s) 519
AccI GTMKAC 2 cut(s) 186, 349
AccII CGCG 1 cut(s) 195
AciI CCGC 3 cut(s) 33, 193, 238
AclWI GGATC 3 cut(s) 37, 79, 811
AcsI RAATTY 2 cut(s) 429, 830
AcuI CTGAAG 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 149, 521, 800
AfiI CCNNNNNNNGG 3 cut(s) 121, 252, 887
AgsI TTSAA 5 cut(s) 333, 402, 428, 493, 762
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 1 cut(s) 881
AluBI AGCT 2 cut(s) 61, 613
AluI AGCT 2 cut(s) 61, 613
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 2 cut(s) 101, 113
AlwI GGATC 3 cut(s) 37, 79, 811
AlwNI CAGNNNCTG 1 cut(s) 821
Ama87I CYCGRG 2 cut(s) 116, 794
AoxI GGCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 2 cut(s) 429, 830
ArsI GACNNNNNNTTYG 2 cut(s) 761, 793
Asp700I GAANNNNTTC 2 cut(s) 708, 890
Asp718I GGTACC 1 cut(s) 519
AspLEI GCGC 2 cut(s) 180, 678
AspS9I GGNCC 2 cut(s) 163, 474
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 3 cut(s) 197, 869, 881
AvaI CYCGRG 2 cut(s) 116, 794
AvaII GGWCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 519
BanII GRGCYC 2 cut(s) 40, 63
BbsI GAAGAC 2 cut(s) 390, 868
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 67
BccI CCATC 6 cut(s) 113, 254, 510, 617, 638, 860
BceAI ACGGC 4 cut(s) 93, 138, 230, 761
BciT130I CCWGG 1 cut(s) 883
BclI TGATCA 1 cut(s) 202
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 101, 113
BfaI CTAG 2 cut(s) 273, 461
BisI GCNGC 2 cut(s) 81, 238
BlsI GCNGC 2 cut(s) 82, 239
Bme1390I CCNGG 2 cut(s) 65, 883
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 2 cut(s) 116, 794
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 163, 474
BmiI GGNNCC 5 cut(s) 37, 103, 165, 475, 521
BmrFI CCNGG 2 cut(s) 65, 883
BmsI GCATC 3 cut(s) 189, 772, 850
BpiI GAAGAC 2 cut(s) 390, 868
BpmI CTGGAG 2 cut(s) 578, 719
BpuEI CTTGAG 1 cut(s) 680
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 881
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 252, 887
Bse1I ACTGG 2 cut(s) 56, 679
Bse3DI GCAATG 1 cut(s) 436
BseBI CCWGG 1 cut(s) 883
BseDI CCNNGG 1 cut(s) 881
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 3 cut(s) 121, 252, 887
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 56, 679
BseRI GAGGAG 2 cut(s) 299, 551
BseXI GCAGC 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 1 cut(s) 476
BshNI GGYRCC 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 63
BsiHKCI CYCGRG 2 cut(s) 116, 794
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 3 cut(s) 121, 252, 887
BsmAI GTCTC 2 cut(s) 101, 113
BsmBI CGTCTC 2 cut(s) 101, 113
BsnI GGCC 1 cut(s) 476
BsoBI CYCGRG 2 cut(s) 116, 794
Bsp1286I GDGCHC 2 cut(s) 40, 63
Bsp143I GATC 6 cut(s) 42, 84, 202, 250, 670, 803
BspACI CCGC 3 cut(s) 33, 193, 238
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 1 cut(s) 139
BspFNI CGCG 1 cut(s) 195
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 5 cut(s) 37, 103, 165, 475, 521
BspPI GGATC 3 cut(s) 37, 79, 811
BspT107I GGYRCC 1 cut(s) 519
BsrDI GCAATG 1 cut(s) 436
BsrI ACTGG 2 cut(s) 56, 679
BssECI CCNNGG 1 cut(s) 881
BssMI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 883
Bst4CI ACNGT 3 cut(s) 414, 512, 524
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 4 cut(s) 443, 552, 629, 633
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 2 cut(s) 180, 678
BstKTI GATC 6 cut(s) 45, 87, 205, 253, 673, 806
BstMAI GTCTC 2 cut(s) 101, 113
BstMBI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
BstNI CCWGG 1 cut(s) 883
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 63, 881
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 2 cut(s) 390, 868
BsuRI GGCC 1 cut(s) 476
BtgZI GCGATG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 5 cut(s) 321, 399, 410, 497, 686
Cac8I GCNNGC 4 cut(s) 443, 552, 629, 633
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 1 cut(s) 217
CfoI GCGC 2 cut(s) 180, 678
Cfr13I GGNCC 2 cut(s) 163, 474
Csp6I GTAC 3 cut(s) 148, 520, 799
CviAII CATG 7 cut(s) 30, 172, 218, 452, 533, 551, 850
CviQI GTAC 3 cut(s) 148, 520, 799
DdeI CTNAG 1 cut(s) 126
DpnI GATC 6 cut(s) 44, 86, 204, 252, 672, 805
DpnII GATC 6 cut(s) 42, 84, 202, 250, 670, 803
Ecl136II GAGCTC 1 cut(s) 61
Eco24I GRGCYC 2 cut(s) 40, 63
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 843
Eco88I CYCGRG 2 cut(s) 116, 794
EcoICRI GAGCTC 1 cut(s) 61
EcoO109I RGGNCCY 2 cut(s) 163, 474
EcoRII CCWGG 1 cut(s) 881
EcoT22I ATGCAT 1 cut(s) 579
EcoT38I GRGCYC 2 cut(s) 40, 63
Esp3I CGTCTC 2 cut(s) 101, 113
FaeI CATG 7 cut(s) 33, 175, 221, 455, 536, 554, 853
FatI CATG 7 cut(s) 29, 171, 217, 451, 532, 550, 849
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 2 cut(s) 186, 349
Fnu4HI GCNGC 2 cut(s) 81, 238
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 2 cut(s) 40, 63
Fsp4HI GCNGC 2 cut(s) 81, 238
FspBI CTAG 2 cut(s) 273, 461
GlaI GCGC 2 cut(s) 179, 677
GluI GCNGC 2 cut(s) 81, 238
GsuI CTGGAG 2 cut(s) 578, 719
HaeIII GGCC 1 cut(s) 476
HapII CCGG 1 cut(s) 65
HhaI GCGC 2 cut(s) 180, 678
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 7 cut(s) 33, 175, 221, 455, 536, 554, 853
Hin6I GCGC 2 cut(s) 178, 676
HinP1I GCGC 2 cut(s) 178, 676
HincII GTYRAC 2 cut(s) 187, 769
HindII GTYRAC 2 cut(s) 187, 769
HinfI GANTC 3 cut(s) 287, 662, 758
HpaII CCGG 1 cut(s) 65
HphI GGTGA 3 cut(s) 197, 869, 881
Hpy166II GTNNAC 3 cut(s) 187, 350, 769
Hpy188I TCNGA 7 cut(s) 47, 101, 129, 255, 344, 714, 808
Hpy8I GTNNAC 3 cut(s) 187, 350, 769
Hpy99I CGWCG 2 cut(s) 188, 200
HpyAV CCTTC 3 cut(s) 21, 124, 301
HpyCH4III ACNGT 3 cut(s) 414, 512, 524
HpyCH4IV ACGT 3 cut(s) 72, 143, 788
HpyCH4V TGCA 8 cut(s) 138, 159, 366, 392, 554, 577, 631, 863
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 3 cut(s) 72, 143, 788
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 7 cut(s) 33, 175, 221, 455, 536, 554, 853
HspAI GCGC 2 cut(s) 178, 676
KpnI GGTACC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 6 cut(s) 42, 84, 202, 250, 670, 803
LmnI GCTCC 5 cut(s) 35, 58, 66, 101, 255
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 3 cut(s) 189, 772, 850
MaeI CTAG 2 cut(s) 273, 461
MaeII ACGT 3 cut(s) 72, 143, 788
MaeIII GTNAC 4 cut(s) 73, 542, 663, 683
MalI GATC 6 cut(s) 44, 86, 204, 252, 672, 805
MboI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
MboII GAAGA 4 cut(s) 241, 390, 716, 868
MfeI CAATTG 1 cut(s) 762
MhlI GDGCHC 2 cut(s) 40, 63
MluCI AATT 5 cut(s) 429, 580, 732, 762, 830
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 1 cut(s) 396
Mph1103I ATGCAT 1 cut(s) 579
MroXI GAANNNNTTC 2 cut(s) 708, 890
MseI TTAA 1 cut(s) 735
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 65, 883
MunI CAATTG 1 cut(s) 762
MvaI CCWGG 1 cut(s) 883
MvnI CGCG 1 cut(s) 195
NciI CCSGG 1 cut(s) 65
NdeII GATC 6 cut(s) 42, 84, 202, 250, 670, 803
NlaIII CATG 7 cut(s) 33, 175, 221, 455, 536, 554, 853
NlaIV GGNNCC 5 cut(s) 37, 103, 165, 475, 521
NmuCI GTSAC 2 cut(s) 73, 663
NsiI ATGCAT 1 cut(s) 579
NspI RCATGY 1 cut(s) 554
PaeI GCATGC 1 cut(s) 554
PaeR7I CTCGAG 2 cut(s) 116, 794
PagI TCATGA 1 cut(s) 217
PcsI WCGNNNNNNNCGW 5 cut(s) 105, 114, 192, 358, 520
PdmI GAANNNNTTC 2 cut(s) 708, 890
PfeI GAWTC 2 cut(s) 287, 758
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 2 cut(s) 82, 239
PleI GAGTC 1 cut(s) 670
PpsI GAGTC 1 cut(s) 670
PpuMI RGGWCCY 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 881
PspGI CCWGG 1 cut(s) 881
PspN4I GGNNCC 5 cut(s) 37, 103, 165, 475, 521
PspPI GGNCC 2 cut(s) 163, 474
PspPPI RGGWCCY 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 821
RsaI GTAC 3 cut(s) 149, 521, 800
RsaNI GTAC 3 cut(s) 148, 520, 799
SacI GAGCTC 1 cut(s) 63
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 2 cut(s) 81, 238
Sau3AI GATC 6 cut(s) 42, 84, 202, 250, 670, 803
Sau96I GGNCC 2 cut(s) 163, 474
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 2 cut(s) 65, 883
SduI GDGCHC 2 cut(s) 40, 63
SfaNI GCATC 3 cut(s) 189, 772, 850
Sfr274I CTCGAG 2 cut(s) 116, 794
SinI GGWCC 1 cut(s) 163
SlaI CTCGAG 2 cut(s) 116, 794
SmlI CTYRAG 3 cut(s) 116, 659, 794
SmoI CTYRAG 3 cut(s) 116, 659, 794
SphI GCATGC 1 cut(s) 554
Sse9I AATT 5 cut(s) 429, 580, 732, 762, 830
SsiI CCGC 3 cut(s) 33, 193, 238
SspMI CTAG 2 cut(s) 273, 461
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 2 cut(s) 63, 881
TaaI ACNGT 3 cut(s) 414, 512, 524
TaiI ACGT 3 cut(s) 75, 146, 791
TaqI TCGA 7 cut(s) 108, 117, 186, 285, 514, 795, 828
TasI AATT 5 cut(s) 429, 580, 732, 762, 830
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 2 cut(s) 287, 758
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 5 cut(s) 328, 399, 417, 504, 686
TseFI GTSAC 2 cut(s) 73, 663
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 2 cut(s) 73, 663
TspDTI ATGAA 3 cut(s) 206, 468, 838
TspGWI ACGGA 2 cut(s) 71, 816
TspRI CASTG 5 cut(s) 328, 399, 417, 504, 686
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 2 cut(s) 429, 830
XbaI TCTAGA 1 cut(s) 460
XceI RCATGY 1 cut(s) 554
XhoI CTCGAG 2 cut(s) 116, 794
XmiI GTMKAC 2 cut(s) 186, 349
XmnI GAANNNNTTC 2 cut(s) 708, 890
XspI CTAG 2 cut(s) 273, 461
ZraI GACGTC 1 cut(s) 73
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.