RLG00000032274

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018813
Physical Location & Seq
Reverse (-)
12978501 .. 12981191
2691 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032274

Sequence Viewer

Length: 2319 bp
ATGTTAGGAGTAGTAGTACCAATATTAGTGGTGGTTGTATTAGCGGCTGCAACAAGAAGTAGTACAACATCTGCACAAGCCTTGCCTAACTGTCCCGATCGCTGCGGTGATGTGAAAATCCCATATCCATTTGGCACGGTTAAAGGTTGTTACCTGCGAGATGAGTTTTTTATCAACTGTACTAGTACTGAAGGCAATGCCTCAGTAGCAAAACTCACGGGTCCTCCCTCGTCGTCACCAACATCACCATTGACTGATCTCATTGTTACTGACATAAGCCTTGACAACGCTGAGTTGCGTATCTTGTCCTCTGTATACCAAGACTGCGGCGACCGCAAAGAGACCCTGAACCAGTGGCTTCGGCTGCCCTCTCCTTTTTCATTTTCCACCAAAAACATCTTCTTTGCCGTCGGCTGTGCCACTTTTGCAAAATATCGAGGTTACCAATATCGTCCCTTCGGCAGGATCCTGGGCCCAGATGAAGATCAAGCCTACAACAAAAGCGGCTTTGCCGTAACCCAATGTGATGATAGTCTTGGCACCACTTGCTCTGGCAATGGCTGTTCCCAATCTTCAATCCCGAGTGGACTGCAAAATTTCACTGTTGAGGCTTTGTTAAGCATCAGCATAAATGGCTATATCCCTAATCAGGGAAAGTGGTACAGTCCATACATAGGGTGCAACTACGGCTTCCTTGCGGAAGAAGGCAACTTCTCATTCTCCCCCAATATAAGTTTTGCCCAACTCCAAAAGTACAGAAGGCTGCTGGTCCCAACCACAACGGTGGTTAATTGGCAAATTGGGGATGAGCCATGTGAAGAAGCCCAAAAGAGCAAGGGAAGTTATGCTTGCAAGGGAAATAGCACGTGCGTCAATTGGTCCAATGTCAATGAGGGTGGATATGGTGGATGGCATCTAGGCTACATTTGCCGGTGCTTGCCAGGTTACCAGGGGAATCCATACCTTGCAGATGGTTGTCAAGATATCAGTGAGTGTCAGAGTTCAAACCGCTGCAGCACAGAAGAACTCTGCGTAAATCTACCAGGACAGTATAAATGTGATCCCAATCAATTGAAGACATCTCTGAGGTTTTTAATTTCATTTGGTATCTTTGCATCAGTCTTTGTGATAGTGGTTGGAGGTTTAGGGTCGATATATTGGGAAATGAAGAAACGAAAGTACATGATTTTGAAAGAAAAGTACTTCAAAAAAAATGGTGGTTTATTGTTACAACAGCTTGCTAATCATCATGGAGTGGAGACTGCAAAAATATTTACTGCAGAAGAACTTGAGAAGGCCACAAACAATTATCATGAAAGCAGAGTCCTTGGTGAAGGAGGTTGTGGAATAGTTTACAAGGGAATATTGCAGAATGACAGAACGGTTGCCATAAAGAAGCCCAAAGTTGGTGACTCATCCCACAGAAGTTCTACCCCAACCCACAGCACTCCCAGTCAGCAGTTTGTTAATGAAGTGATTATTCTTTCACAGATCAACCACATAAATGTGGTGAGGTTATTGGGTTGTTGCTTGGAGACATCGGTGCCTACACTCGTTTATGAGTTCATCACTAATGGCACCCTTTTTAAGCACCTTCATGGAGATCGAGATAAAGAGGGAAAAAGATCATCACCACCACTTTTTTGGGATTTACGATTAAAGATAGCAACAGAAACTGCAGGTGCATTGGCATACTTACACTCCTCCACTTCGACGCCAATATTACATCGAGATGTGAAGACAATGAACATACTACTAGATGAAAACTACACTGCAAAAGTCGCTGACTTTGGAGCTTCACAATTGATTCGACCTATTGATGAAAAGCAAATGACAACTTTTGTGCAAGGGACACTCGGATACTTAGACCCTGAATATTTTCACTCGGATGAATTTACAGAAAAAAGCGACGTCTATAGCTTTGGAGTTGTCCTAGTGGAGCTACTGACAAGTAAAAAGGCATTTTCTTTTGCTAGGCCAGATGCAGAGAGAAACCTTTCAAAATTGTTTGTTTCTTCGGTGGAAGACGGTCGCTTGAAGGAAATTCTTGACAATGACATGGTCGACGAGGCAAATGTTAATGATACAATGGTACTAGAAAATGTGGCTTATCTAGCAAGGAGATGTTTGAGAGTAAAAGGGGACGAAAGGCCTACTATGAAAGAAGTAGCCATGGAGCTAGAGTGTTTATATAAAGGATTGAGTCCTATTTATGATATGACACATGAGCATTACTTTATTTGTCAAGACGAGACTGATGAGGACTTGCTGGGATCATCAACTTCAAATACTGCTGACGTTGCTGCAGATGTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

773

Amino Acids

85.44

Weight (kDa)

5.6

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 101 8.7e-07 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 437 - 723 4.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 440 - 727 2.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018813)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10620
rosa_chinensis RchiOBHm_Chr5g0017331
rosa_laevigata RLG00000032274
rosa_multiflora Rmu_co8169364.1_g000001 Rmu_sc0005223.1_g000006 Rmu_sc0005223.1_g000026 Rmu_sc0005319.1_g000009
rosa_roxburghii Rroxscaffold_1G00060030
rosa_rugosa Rorug05G0034000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1670
AatII GACGTC 1 cut(s) 1914
Acc36I ACCTGC 2 cut(s) 162, 1670
AccB1I GGYRCC 3 cut(s) 539, 1543, 1577
AccI GTMKAC 2 cut(s) 315, 2064
AciI CCGC 7 cut(s) 44, 105, 327, 334, 504, 698, 1009
AclWI GGATC 4 cut(s) 460, 473, 1055, 2281
AcsI RAATTY 3 cut(s) 595, 1892, 2043
AcuI CTGAAG 1 cut(s) 210
AcvI CACGTG 1 cut(s) 867
AcyI GRCGYC 2 cut(s) 1715, 1911
AfaI GTAC 9 cut(s) 18, 64, 181, 187, 662, 755, 1181, 1202, 2094
AfiI CCNNNNNNNGG 5 cut(s) 462, 649, 650, 674, 1406
AgsI TTSAA 8 cut(s) 576, 1005, 1075, 1192, 1207, 2001, 2038, 2286
AhlI ACTAGT 1 cut(s) 182
AjnI CCWGG 4 cut(s) 468, 940, 948, 1042
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AleI CACNNNNGTG 1 cut(s) 782
AluBI AGCT 5 cut(s) 1237, 1796, 1920, 1942, 2179
AluI AGCT 5 cut(s) 1237, 1796, 1920, 1942, 2179
Alw26I GTCTC 4 cut(s) 335, 1253, 1529, 2246
AlwI GGATC 4 cut(s) 460, 473, 1055, 2281
AlwNI CAGNNNCTG 1 cut(s) 1676
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 4 cut(s) 472, 1296, 1976, 2150
ApaI GGGCCC 1 cut(s) 476
ApeKI GCWGC 7 cut(s) 47, 102, 364, 763, 1011, 1014, 2303
ApoI RAATTY 3 cut(s) 595, 1892, 2043
Asp700I GAANNNNTTC 2 cut(s) 1878, 1996
AspS9I GGNCC 5 cut(s) 221, 472, 473, 769, 879
AsuHPI GGTGA 7 cut(s) 119, 228, 237, 1343, 1421, 1522, 1623
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 3 cut(s) 221, 769, 879
BaeGI GKGCMC 1 cut(s) 476
BaeI ACNNNNGTAYC 2 cut(s) 2076, 2109
BamHI GGATCC 1 cut(s) 465
BanI GGYRCC 3 cut(s) 539, 1543, 1577
BanII GRGCYC 1 cut(s) 476
BbrPI CACGTG 1 cut(s) 867
BbsI GAAGAC 3 cut(s) 1082, 1745, 2031
BbvI GCAGC 7 cut(s) 34, 89, 351, 750, 998, 1026, 2290
BccI CCATC 2 cut(s) 903, 965
BceAI ACGGC 3 cut(s) 392, 497, 703
BcgI CGANNNNNNTGC 2 cut(s) 571, 605
BciT130I CCWGG 4 cut(s) 470, 942, 950, 1044
BciVI GTATCC 1 cut(s) 1853
BcoDI GTCTC 4 cut(s) 335, 1253, 1529, 2246
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 8 cut(s) 183, 917, 1757, 1934, 1974, 2096, 2114, 2180
BfmI CTRYAG 5 cut(s) 1012, 1278, 1677, 1915, 2304
BfuAI ACCTGC 2 cut(s) 162, 1670
BfuI GTATCC 1 cut(s) 1853
BmcAI AGTACT 2 cut(s) 187, 1202
Bme1390I CCNGG 4 cut(s) 470, 942, 950, 1044
Bme18I GGWCC 3 cut(s) 221, 769, 879
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 5 cut(s) 221, 472, 473, 769, 879
BmiI GGNNCC 7 cut(s) 222, 467, 474, 541, 771, 1545, 1579
BmrFI CCNGG 4 cut(s) 470, 942, 950, 1044
BmrI ACTGGG 1 cut(s) 1446
BmsI GCATC 4 cut(s) 630, 922, 1124, 1972
BmuI ACTGGG 1 cut(s) 1446
BpiI GAAGAC 3 cut(s) 1082, 1745, 2031
BpuEI CTTGAG 1 cut(s) 1310
BsaAI YACGTR 1 cut(s) 867
BsaBI GATNNNNATC 2 cut(s) 483, 1065
BsaHI GRCGYC 2 cut(s) 1715, 1911
BsaI GGTCTC 1 cut(s) 335
BsaJI CCNNGG 4 cut(s) 469, 949, 1327, 2172
BsaXI ACNNNNNCTCC 7 cut(s) 30, 208, 238, 1685, 1715, 2168, 2198
Bsc4I CCNNNNNNNGG 5 cut(s) 462, 649, 650, 674, 1406
Bse118I RCCGGY 1 cut(s) 930
Bse1I ACTGG 2 cut(s) 352, 1452
Bse3DI GCAATG 2 cut(s) 202, 562
Bse8I GATNNNNATC 2 cut(s) 483, 1065
BseBI CCWGG 4 cut(s) 470, 942, 950, 1044
BseDI CCNNGG 4 cut(s) 469, 949, 1327, 2172
BseGI GGATG 4 cut(s) 811, 914, 1415, 1894
BseJI GATNNNNATC 2 cut(s) 483, 1065
BseLI CCNNNNNNNGG 5 cut(s) 462, 649, 650, 674, 1406
BseMI GCAATG 2 cut(s) 202, 562
BseMII CTCAG 3 cut(s) 216, 282, 1076
BseNI ACTGG 2 cut(s) 352, 1452
BseRI GAGGAG 1 cut(s) 1693
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 7 cut(s) 34, 89, 351, 750, 998, 1026, 2290
BseYI CCCAGC 1 cut(s) 2269
BsgI GTGCAG 1 cut(s) 57
Bsh1285I CGRYCG 3 cut(s) 100, 334, 2032
BshFI GGCC 4 cut(s) 474, 1298, 1978, 2152
BshNI GGYRCC 3 cut(s) 539, 1543, 1577
BsiEI CGRYCG 3 cut(s) 100, 334, 2032
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 1 cut(s) 931
BslFI GGGAC 5 cut(s) 78, 438, 755, 1864, 2156
BslI CCNNNNNNNGG 5 cut(s) 462, 649, 650, 674, 1406
BsmAI GTCTC 4 cut(s) 335, 1253, 1529, 2246
BsmFI GGGAC 5 cut(s) 78, 438, 755, 1864, 2156
BsnI GGCC 4 cut(s) 474, 1298, 1978, 2152
Bso31I GGTCTC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 580
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
Bsp19I CCATGG 1 cut(s) 2172
BspACI CCGC 7 cut(s) 44, 105, 327, 334, 504, 698, 1009
BspANI GGCC 4 cut(s) 474, 1298, 1978, 2152
BspCNI CTCAG 3 cut(s) 215, 283, 1077
BspHI TCATGA 1 cut(s) 1312
BspLI GGNNCC 7 cut(s) 222, 467, 474, 541, 771, 1545, 1579
BspMAI CTGCAG 4 cut(s) 1016, 1282, 1681, 2308
BspMI ACCTGC 2 cut(s) 162, 1670
BspPI GGATC 4 cut(s) 460, 473, 1055, 2281
BspT107I GGYRCC 3 cut(s) 539, 1543, 1577
BspTNI GGTCTC 1 cut(s) 335
BsrDI GCAATG 2 cut(s) 202, 562
BsrFI RCCGGY 1 cut(s) 930
BsrI ACTGG 2 cut(s) 352, 1452
BssAI RCCGGY 1 cut(s) 930
BssECI CCNNGG 4 cut(s) 469, 949, 1327, 2172
BssMI GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
BssNAI GTATAC 1 cut(s) 316
BssNI GRCGYC 2 cut(s) 1715, 1911
BssT1I CCWWGG 2 cut(s) 1327, 2172
Bst1107I GTATAC 1 cut(s) 316
Bst2UI CCWGG 4 cut(s) 470, 942, 950, 1044
Bst4CI ACNGT 9 cut(s) 92, 139, 179, 604, 665, 784, 1050, 1384, 2030
BstACI GRCGYC 2 cut(s) 1715, 1911
BstAPI GCANNNNNTGC 1 cut(s) 546
BstBAI YACGTR 1 cut(s) 867
BstC8I GCNNGC 3 cut(s) 850, 938, 1239
BstDEI CTNAG 4 cut(s) 202, 291, 1085, 1864
BstDSI CCRYGG 1 cut(s) 2172
BstEII GGTNACC 2 cut(s) 440, 944
BstENI CCTNNNNNAGG 1 cut(s) 460
BstF5I GGATG 4 cut(s) 811, 914, 1415, 1894
BstKTI GATC 9 cut(s) 100, 259, 468, 487, 1063, 1494, 1606, 1628, 2276
BstMAI GTCTC 4 cut(s) 335, 1253, 1529, 2246
BstMBI GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
BstMCI CGRYCG 3 cut(s) 100, 334, 2032
BstNI CCWGG 4 cut(s) 470, 942, 950, 1044
BstPI GGTNACC 2 cut(s) 440, 944
BstSCI CCNGG 4 cut(s) 468, 940, 948, 1042
BstSFI CTRYAG 5 cut(s) 1012, 1278, 1677, 1915, 2304
BstSLI GKGCMC 1 cut(s) 476
BstV1I GCAGC 7 cut(s) 34, 89, 351, 750, 998, 1026, 2290
BstV2I GAAGAC 3 cut(s) 1082, 1745, 2031
BstX2I RGATCY 1 cut(s) 465
BstXI CCANNNNNNTGG 2 cut(s) 784, 1644
BstYI RGATCY 1 cut(s) 465
BstZ17I GTATAC 1 cut(s) 316
BsuI GTATCC 1 cut(s) 1853
BsuRI GGCC 4 cut(s) 474, 1298, 1978, 2152
BtgI CCRYGG 1 cut(s) 2172
BtsCI GGATG 4 cut(s) 811, 914, 1415, 1894
BtsI GCAGTG 1 cut(s) 1770
BtsIMutI CAGTG 4 cut(s) 359, 600, 994, 1770
BveI ACCTGC 2 cut(s) 162, 1670
Cac8I GCNNGC 3 cut(s) 850, 938, 1239
CaiI CAGNNNCTG 1 cut(s) 1676
CciI TCATGA 1 cut(s) 1312
Cfr10I RCCGGY 1 cut(s) 930
Cfr13I GGNCC 5 cut(s) 221, 472, 473, 769, 879
CseI GACGC 2 cut(s) 859, 1723
Csp6I GTAC 9 cut(s) 17, 63, 180, 186, 661, 754, 1180, 1201, 2093
CspCI CAANNNNNGTGG 4 cut(s) 9, 44, 877, 912
CviAII CATG 8 cut(s) 813, 1183, 1250, 1313, 1598, 2059, 2173, 2225
CviQI GTAC 9 cut(s) 17, 63, 180, 186, 661, 754, 1180, 1201, 2093
DdeI CTNAG 4 cut(s) 202, 291, 1085, 1864
DpnI GATC 9 cut(s) 99, 258, 467, 486, 1062, 1493, 1605, 1627, 2275
DpnII GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
Eco130I CCWWGG 2 cut(s) 1327, 2172
Eco147I AGGCCT 1 cut(s) 2152
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 1 cut(s) 335
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 3 cut(s) 221, 769, 879
Eco57I CTGAAG 1 cut(s) 210
Eco72I CACGTG 1 cut(s) 867
Eco88I CYCGRG 1 cut(s) 580
Eco91I GGTNACC 2 cut(s) 440, 944
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 221
EcoO65I GGTNACC 2 cut(s) 440, 944
EcoRII CCWGG 4 cut(s) 468, 940, 948, 1042
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 2 cut(s) 1327, 2172
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 2 cut(s) 1327, 2172
FaeI CATG 8 cut(s) 816, 1186, 1253, 1316, 1601, 2062, 2176, 2228
FalI AAGNNNNNCTT 2 cut(s) 832, 864
FaqI GGGAC 5 cut(s) 78, 438, 755, 1864, 2156
FatI CATG 8 cut(s) 812, 1182, 1249, 1312, 1597, 2058, 2172, 2224
FblI GTMKAC 2 cut(s) 315, 2064
FokI GGATG 4 cut(s) 818, 921, 1402, 1901
FriOI GRGCYC 1 cut(s) 476
FspBI CTAG 8 cut(s) 183, 917, 1757, 1934, 1974, 2096, 2114, 2180
GsaI CCCAGC 1 cut(s) 2273
HaeIII GGCC 4 cut(s) 474, 1298, 1978, 2152
HapII CCGG 1 cut(s) 931
HgaI GACGC 2 cut(s) 859, 1723
Hin1I GRCGYC 2 cut(s) 1715, 1911
Hin1II CATG 8 cut(s) 816, 1186, 1253, 1316, 1601, 2062, 2176, 2228
HincII GTYRAC 1 cut(s) 2065
HindII GTYRAC 1 cut(s) 2065
HinfI GANTC 5 cut(s) 955, 1323, 1412, 1807, 2203
HpaII CCGG 1 cut(s) 931
HphI GGTGA 7 cut(s) 119, 228, 237, 1343, 1421, 1522, 1623
Hpy166II GTNNAC 4 cut(s) 316, 587, 1354, 2065
Hpy188I TCNGA 4 cut(s) 999, 1086, 1859, 1888
Hpy188III TCNNGA 8 cut(s) 95, 580, 980, 1313, 1607, 1730, 2048, 2246
Hpy8I GTNNAC 4 cut(s) 316, 587, 1354, 2065
Hpy99I CGWCG 5 cut(s) 235, 413, 1717, 1913, 2069
HpyAV CCTTC 8 cut(s) 185, 466, 698, 753, 1288, 1328, 1604, 2032
HpyCH4III ACNGT 9 cut(s) 92, 139, 179, 604, 665, 784, 1050, 1384, 2030
HpyCH4IV ACGT 3 cut(s) 866, 1911, 2298
HpyF3I CTNAG 4 cut(s) 202, 291, 1085, 1864
HpySE526I ACGT 3 cut(s) 866, 1911, 2298
Hsp92I GRCGYC 2 cut(s) 1715, 1911
Hsp92II CATG 8 cut(s) 816, 1186, 1253, 1316, 1601, 2062, 2176, 2228
Kzo9I GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
LmnI GCTCC 3 cut(s) 1793, 1939, 2176
Lsp1109I GCAGC 7 cut(s) 34, 89, 351, 750, 998, 1026, 2290
LweI GCATC 4 cut(s) 630, 922, 1124, 1972
MaeI CTAG 8 cut(s) 183, 917, 1757, 1934, 1974, 2096, 2114, 2180
MaeII ACGT 3 cut(s) 866, 1911, 2298
MaeIII GTNAC 8 cut(s) 149, 234, 265, 440, 514, 944, 1227, 1409
MalI GATC 9 cut(s) 99, 258, 467, 486, 1062, 1493, 1605, 1627, 2275
MboI GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
MfeI CAATTG 3 cut(s) 874, 1070, 1802
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 476
MlyI GAGTC 3 cut(s) 1332, 1406, 2212
MmeI TCCRAC 1 cut(s) 1117
MroXI GAANNNNTTC 2 cut(s) 1878, 1996
MseI TTAA 8 cut(s) 141, 617, 789, 1094, 1467, 1587, 1658, 2079
MslI CAYNNNNRTG 6 cut(s) 782, 1503, 1505, 1596, 1731, 1887
MspA1I CMGCKG 1 cut(s) 1011
MspI CCGG 1 cut(s) 931
MspR9I CCNGG 4 cut(s) 470, 942, 950, 1044
MunI CAATTG 3 cut(s) 874, 1070, 1802
MvaI CCWGG 4 cut(s) 470, 942, 950, 1044
NcoI CCATGG 1 cut(s) 2172
NdeII GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
NlaIII CATG 8 cut(s) 816, 1186, 1253, 1316, 1601, 2062, 2176, 2228
NlaIV GGNNCC 7 cut(s) 222, 467, 474, 541, 771, 1545, 1579
NmuCI GTSAC 2 cut(s) 234, 1409
OliI CACNNNNGTG 1 cut(s) 782
PagI TCATGA 1 cut(s) 1312
PaqCI CACCTGC 1 cut(s) 1670
PceI AGGCCT 1 cut(s) 2152
PdmI GAANNNNTTC 2 cut(s) 1878, 1996
PfeI GAWTC 2 cut(s) 955, 1807
PflFI GACNNNGTC 1 cut(s) 2060
Ple19I CGATCG 1 cut(s) 100
PleI GAGTC 3 cut(s) 1331, 1406, 2211
PmaCI CACGTG 1 cut(s) 867
PmlI CACGTG 1 cut(s) 867
PpsI GAGTC 3 cut(s) 1331, 1406, 2211
Ppu21I YACGTR 1 cut(s) 867
PpuMI RGGWCCY 1 cut(s) 221
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 4 cut(s) 468, 940, 948, 1042
PspCI CACGTG 1 cut(s) 867
PspEI GGTNACC 2 cut(s) 440, 944
PspFI CCCAGC 1 cut(s) 2269
PspGI CCWGG 4 cut(s) 468, 940, 948, 1042
PspN4I GGNNCC 7 cut(s) 222, 467, 474, 541, 771, 1545, 1579
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 5 cut(s) 221, 472, 473, 769, 879
PspPPI RGGWCCY 1 cut(s) 221
PstI CTGCAG 4 cut(s) 1016, 1282, 1681, 2308
PstNI CAGNNNCTG 1 cut(s) 1676
PsuI RGATCY 1 cut(s) 465
PsyI GACNNNGTC 1 cut(s) 2060
PvuI CGATCG 1 cut(s) 100
RsaI GTAC 9 cut(s) 18, 64, 181, 187, 662, 755, 1181, 1202, 2094
RsaNI GTAC 9 cut(s) 17, 63, 180, 186, 661, 754, 1180, 1201, 2093
RseI CAYNNNNRTG 6 cut(s) 782, 1503, 1505, 1596, 1731, 1887
SalI GTCGAC 1 cut(s) 2063
SaqAI TTAA 8 cut(s) 141, 617, 789, 1094, 1467, 1587, 1658, 2079
Sau3AI GATC 9 cut(s) 97, 256, 465, 484, 1060, 1491, 1603, 1625, 2273
Sau96I GGNCC 5 cut(s) 221, 472, 473, 769, 879
ScaI AGTACT 2 cut(s) 187, 1202
SchI GAGTC 3 cut(s) 1332, 1406, 2212
ScrFI CCNGG 4 cut(s) 470, 942, 950, 1044
SduI GDGCHC 1 cut(s) 476
SfaNI GCATC 4 cut(s) 630, 922, 1124, 1972
SfcI CTRYAG 5 cut(s) 1012, 1278, 1677, 1915, 2304
SinI GGWCC 3 cut(s) 221, 769, 879
SmiMI CAYNNNNRTG 6 cut(s) 782, 1503, 1505, 1596, 1731, 1887
SmlI CTYRAG 1 cut(s) 1289
SmoI CTYRAG 1 cut(s) 1289
SpeI ACTAGT 1 cut(s) 182
SseBI AGGCCT 1 cut(s) 2152
SsiI CCGC 7 cut(s) 44, 105, 327, 334, 504, 698, 1009
SspI AATATT 5 cut(s) 24, 1272, 1365, 1722, 1877
SspMI CTAG 8 cut(s) 183, 917, 1757, 1934, 1974, 2096, 2114, 2180
StuI AGGCCT 1 cut(s) 2152
StyD4I CCNGG 4 cut(s) 468, 940, 948, 1042
StyI CCWWGG 2 cut(s) 1327, 2172
TaaI ACNGT 9 cut(s) 92, 139, 179, 604, 665, 784, 1050, 1384, 2030
TaiI ACGT 3 cut(s) 869, 1914, 2301
TaqI TCGA 7 cut(s) 436, 1151, 1606, 1712, 1729, 1810, 2064
TatI WGTACW 6 cut(s) 62, 179, 185, 753, 1179, 1200
TauI GCSGC 3 cut(s) 47, 330, 507
TfiI GAWTC 2 cut(s) 955, 1807
Tru1I TTAA 8 cut(s) 141, 617, 789, 1094, 1467, 1587, 1658, 2079
Tru9I TTAA 8 cut(s) 141, 617, 789, 1094, 1467, 1587, 1658, 2079
TscAI CASTG 4 cut(s) 359, 607, 994, 1777
TseFI GTSAC 2 cut(s) 234, 1409
TseI GCWGC 7 cut(s) 47, 102, 364, 763, 1011, 1014, 2303
Tsp45I GTSAC 2 cut(s) 234, 1409
TspRI CASTG 4 cut(s) 359, 607, 994, 1777
Tth111I GACNNNGTC 1 cut(s) 2060
VpaK11BI GGWCC 3 cut(s) 221, 769, 879
XagI CCTNNNNNAGG 1 cut(s) 460
XapI RAATTY 3 cut(s) 595, 1892, 2043
XcmI CCANNNNNNNNNTGG 1 cut(s) 1641
XmiI GTMKAC 2 cut(s) 315, 2064
XmnI GAANNNNTTC 2 cut(s) 1878, 1996
XspI CTAG 8 cut(s) 183, 917, 1757, 1934, 1974, 2096, 2114, 2180
ZraI GACGTC 1 cut(s) 1912
ZrmI AGTACT 2 cut(s) 187, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.