Rmu_sc0005223.1_g000026

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005223.1
OG0018813
Physical Location & Seq
Forward (+)
90401 .. 91641
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005223.1_g000026.1.cds

Sequence Viewer

Length: 828 bp
atggccaaaatatttactgcagaagaacttgaagagaccacaaacaattatcatgaaatcagagtccttggtgaaggaggctgtggaatagtttacaagggaatattgcagaataacagaacggttgccataaagaagcccaaagttggtgcctcatcccacagaagtactaccccaacccacagcactcccatgccagtcagcaatagcaacagaaactgcaggtgcattggcatacttacactcctccacttcgacgccaatattacatcgagatgtgaagacaatgaacatactactagattttcctacactgcaaaagtcgctgactttggagcttcacaattgattcgacctcttgatgaaaagcaaatgacaacttttgtgcaagggacactcggatacttggaccctgaatattttcattcggatgaatttacagaaaaaagcgacgtctatagctttggagttgtcctagtggaactactgacaagtaaaaaggcattttcttttgctaggccagatgcagagagaaacctttcaaaactgtttgtttcttcggtggaagacggtcgcttgaaggaaattcttgacaatgacatgatcgatgaggcaaatattaatgatacaatgatgttctttgaggtgatgagccccgaaatccttcacatgggggtgttgcacgaggccgagggtgatgcgtttgtttttccaaaatggacttgtgcaattccagaggctattactgctctcagcagccaagatcctggtgttgtaacaattgctgcactgagcagcccatattcttgtgaaggctttgcaagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

275

Amino Acids

30.46

Weight (kDa)

4.99

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018813)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10620
rosa_chinensis RchiOBHm_Chr5g0017331
rosa_laevigata RLG00000032274
rosa_multiflora Rmu_co8169364.1_g000001 Rmu_sc0005223.1_g000006 Rmu_sc0005223.1_g000026 Rmu_sc0005319.1_g000009
rosa_roxburghii Rroxscaffold_1G00060030
rosa_rugosa Rorug05G0034000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 213
AatII GACGTC 1 cut(s) 456
Acc36I ACCTGC 1 cut(s) 213
AccB1I GGYRCC 1 cut(s) 149
AclWI GGATC 1 cut(s) 758
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 434, 585
AcyI GRCGYC 2 cut(s) 258, 453
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 2 cut(s) 146, 670
AgsI TTSAA 3 cut(s) 32, 543, 580
AjnI CCWGG 1 cut(s) 766
AluBI AGCT 2 cut(s) 338, 462
AluI AGCT 2 cut(s) 338, 462
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 758
AlwNI CAGNNNCTG 1 cut(s) 219
AoxI GGCC 3 cut(s) 3, 518, 687
ApeKI GCWGC 3 cut(s) 756, 785, 795
ApoI RAATTY 2 cut(s) 434, 585
AseI ATTAAT 1 cut(s) 621
Asp700I GAANNNNTTC 3 cut(s) 420, 538, 663
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 3 cut(s) 83, 658, 707
AvaII GGWCC 1 cut(s) 409
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 656
BauI CACGAG 1 cut(s) 683
BbsI GAAGAC 2 cut(s) 288, 573
BbvI GCAGC 3 cut(s) 768, 772, 807
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciT130I CCWGG 1 cut(s) 768
BciVI GTATCC 1 cut(s) 395
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 3 cut(s) 300, 476, 516
BfmI CTRYAG 3 cut(s) 18, 220, 457
BfuAI ACCTGC 1 cut(s) 213
BfuI GTATCC 1 cut(s) 395
BisI GCNGC 3 cut(s) 757, 786, 796
BlsI GCNGC 3 cut(s) 758, 787, 797
BmcAI AGTACT 1 cut(s) 169
Bme1390I CCNGG 1 cut(s) 768
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 2 cut(s) 151, 411
BmrFI CCNGG 1 cut(s) 768
BmsI GCATC 2 cut(s) 514, 688
BpiI GAAGAC 2 cut(s) 288, 573
Bsa29I ATCGAT 1 cut(s) 606
BsaHI GRCGYC 2 cut(s) 258, 453
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 67, 690
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 670
Bse1I ACTGG 1 cut(s) 197
BseBI CCWGG 1 cut(s) 768
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 67, 690
BseGI GGATG 2 cut(s) 155, 436
BseLI CCNNNNNNNGG 2 cut(s) 146, 670
BseMII CTCAG 2 cut(s) 766, 782
BseNI ACTGG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 768, 772, 807
BsgI GTGCAG 1 cut(s) 771
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 3 cut(s) 5, 520, 689
BshNI GGYRCC 1 cut(s) 149
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 574
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 2 cut(s) 146, 670
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 406
BsnI GGCC 3 cut(s) 5, 520, 689
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 656
Bsp143I GATC 2 cut(s) 603, 763
BspANI GGCC 3 cut(s) 5, 520, 689
BspCNI CTCAG 2 cut(s) 765, 783
BspDI ATCGAT 1 cut(s) 606
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 151, 411
BspMAI CTGCAG 2 cut(s) 22, 224
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 29
BsrI ACTGG 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 67, 690
BssMI GATC 2 cut(s) 603, 763
BssNI GRCGYC 2 cut(s) 258, 453
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 683
Bst2UI CCWGG 1 cut(s) 768
Bst4CI ACNGT 3 cut(s) 124, 549, 572
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 2 cut(s) 258, 453
BstDEI CTNAG 2 cut(s) 752, 791
BstF5I GGATG 2 cut(s) 155, 436
BstKTI GATC 2 cut(s) 606, 766
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 2 cut(s) 603, 763
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 2 cut(s) 323, 746
BstNI CCWGG 1 cut(s) 768
BstSCI CCNGG 1 cut(s) 766
BstSFI CTRYAG 3 cut(s) 18, 220, 457
BstV1I GCAGC 3 cut(s) 768, 772, 807
BstV2I GAAGAC 2 cut(s) 288, 573
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 1 cut(s) 763
Bsu15I ATCGAT 1 cut(s) 606
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 3 cut(s) 5, 520, 689
BsuTUI ATCGAT 1 cut(s) 606
BtsCI GGATG 2 cut(s) 155, 436
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 312, 788
BveI ACCTGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 219
CciI TCATGA 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 409
ClaI ATCGAT 1 cut(s) 606
CseI GACGC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 168
CviAII CATG 4 cut(s) 53, 193, 601, 670
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 2 cut(s) 752, 791
DpnI GATC 2 cut(s) 605, 765
DpnII GATC 2 cut(s) 603, 763
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 656
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 766
EcoT14I CCWWGG 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 656
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 4 cut(s) 56, 196, 604, 673
FaiI YATR 9 cut(s) 54, 131, 194, 236, 294, 459, 602, 671, 802
FaqI GGGAC 1 cut(s) 406
FatI CATG 4 cut(s) 52, 192, 600, 669
Fnu4HI GCNGC 3 cut(s) 757, 786, 796
FokI GGATG 2 cut(s) 142, 443
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 3 cut(s) 757, 786, 796
FspBI CTAG 3 cut(s) 300, 476, 516
GluI GCNGC 3 cut(s) 757, 786, 796
HaeIII GGCC 3 cut(s) 5, 520, 689
HgaI GACGC 1 cut(s) 266
Hin1I GRCGYC 2 cut(s) 258, 453
Hin1II CATG 4 cut(s) 56, 196, 604, 673
HinfI GANTC 2 cut(s) 63, 349
HphI GGTGA 3 cut(s) 83, 658, 707
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 62, 401, 430
Hpy188III TCNNGA 5 cut(s) 53, 273, 359, 590, 734
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 2 cut(s) 260, 455
HpyAV CCTTC 4 cut(s) 68, 574, 674, 806
HpyCH4III ACNGT 3 cut(s) 124, 549, 572
HpyCH4IV ACGT 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 746
HpyF3I CTNAG 2 cut(s) 752, 791
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 2 cut(s) 258, 453
Hsp92II CATG 4 cut(s) 56, 196, 604, 673
Kzo9I GATC 2 cut(s) 603, 763
LmnI GCTCC 1 cut(s) 335
LpnPI CCDG 7 cut(s) 208, 210, 426, 534, 747, 753, 780
Lsp1109I GCAGC 3 cut(s) 768, 772, 807
LweI GCATC 2 cut(s) 514, 688
MaeI CTAG 3 cut(s) 300, 476, 516
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 775
MalI GATC 2 cut(s) 605, 765
MboI GATC 2 cut(s) 603, 763
MboII GAAGA 5 cut(s) 35, 44, 293, 549, 578
MfeI CAATTG 2 cut(s) 344, 780
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 1 cut(s) 656
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 46, 344, 434, 585, 729, 780
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 72
MnlI CCTC 9 cut(s) 71, 163, 257, 366, 604, 637, 679, 685, 730
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 3 cut(s) 420, 538, 663
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 4 cut(s) 191, 274, 429, 674
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 768
MunI CAATTG 2 cut(s) 344, 780
MvaI CCWGG 1 cut(s) 768
MwoI GCNNNNNNNGC 2 cut(s) 323, 746
NdeII GATC 2 cut(s) 603, 763
NlaIII CATG 4 cut(s) 56, 196, 604, 673
NlaIV GGNNCC 2 cut(s) 151, 411
NmeAIII GCCGAG 1 cut(s) 715
PagI TCATGA 1 cut(s) 52
PaqCI CACCTGC 1 cut(s) 213
PdmI GAANNNNTTC 3 cut(s) 420, 538, 663
PfeI GAWTC 1 cut(s) 349
PkrI GCNGC 3 cut(s) 758, 787, 797
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 766
PspGI CCWGG 1 cut(s) 766
PspN4I GGNNCC 2 cut(s) 151, 411
PspPI GGNCC 1 cut(s) 409
PstI CTGCAG 2 cut(s) 22, 224
PstNI CAGNNNCTG 1 cut(s) 219
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 4 cut(s) 191, 274, 429, 674
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 3 cut(s) 757, 786, 796
Sau3AI GATC 2 cut(s) 603, 763
Sau96I GGNCC 1 cut(s) 409
ScaI AGTACT 1 cut(s) 169
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 1 cut(s) 768
SduI GDGCHC 1 cut(s) 656
SetI ASST 7 cut(s) 227, 340, 358, 456, 464, 540, 648
SfaNI GCATC 2 cut(s) 514, 688
SfcI CTRYAG 3 cut(s) 18, 220, 457
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 4 cut(s) 191, 274, 429, 674
Sse9I AATT 6 cut(s) 46, 344, 434, 585, 729, 780
SspI AATATT 5 cut(s) 12, 105, 265, 419, 619
SspMI CTAG 3 cut(s) 300, 476, 516
StyD4I CCNGG 1 cut(s) 766
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 3 cut(s) 124, 549, 572
TaiI ACGT 1 cut(s) 456
TaqI TCGA 4 cut(s) 255, 272, 352, 606
TasI AATT 6 cut(s) 46, 344, 434, 585, 729, 780
TatI WGTACW 1 cut(s) 167
TfiI GAWTC 1 cut(s) 349
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 2 cut(s) 319, 795
TseI GCWGC 3 cut(s) 756, 785, 795
TspDTI ATGAA 5 cut(s) 69, 303, 378, 413, 447
TspRI CASTG 2 cut(s) 319, 795
VpaK11BI GGWCC 1 cut(s) 409
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 2 cut(s) 434, 585
XmnI GAANNNNTTC 3 cut(s) 420, 538, 663
XspI CTAG 3 cut(s) 300, 476, 516
ZraI GACGTC 1 cut(s) 454
ZrmI AGTACT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.