Rorug05G0034000

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018813
Physical Location & Seq
Forward (+)
2353212 .. 2354095
884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0034000.1

Sequence Viewer

Length: 699 bp
ATGGAATTATACATAGATCCAATCAATCACGGGCATTTGGATTGGGACACACGTTACAAAATTATAGGAGGCATTGCTCGAGGGATTCTCTACCTCCATGAAGATTCTCGACTTCGAATCATCCATCGTGACCTTAAACCCAGCAATATTTTATTAGATGGGGATATGAATCCTAAAATTGCAGATTTTGGCATGGCAAGATTATTTCTGATGGATCAAACTCAAGGACACACAAACAGAGTCGTAGGGACCTATGGATACATGGCTCCAGAGTATGCCATCCATGGGCGTTTCTCTGTCAAATCGGATGTCTTCAGTTTTGGTGTGTTAGTTCTTGAGATTGTGAGTGGCAAGAAAGTGAGCACTTTTCGGAATGGTGAGAATGAAGAGGACCTTCTCAGCTATGCATGGCGAAGTTGGAAAGAGGACACAGTTCATAATATCATAGATCCAATGTTGACAAAGAGTTCAAGGATTGAAACAATGAGATGTATTCACATTGGCTTACTTTGCGTCCAAGAAAATCTGAATAGCAGACCAACTATGTCTTCAGTTGTTTCCATGCTTAATAGCGATTCTGTTACACTCTCGCTACCATTGCGACCTGGATATTTTTTGCAATATAATGATGAATCAGACATCAACGAACTGACAGAGTCAAGAAATGAGGCTTCAATTACGAATCCATATCCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

232

Amino Acids

26.53

Weight (kDa)

5.75

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 10 - 186 5.8e-29 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 12 - 189 1.8e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018813)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10620
rosa_chinensis RchiOBHm_Chr5g0017331
rosa_laevigata RLG00000032274
rosa_multiflora Rmu_co8169364.1_g000001 Rmu_sc0005223.1_g000006 Rmu_sc0005223.1_g000026 Rmu_sc0005319.1_g000009
rosa_roxburghii Rroxscaffold_1G00060030
rosa_rugosa Rorug05G0034000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 11, 222, 443
AcuI CTGAAG 2 cut(s) 298, 534
AfiI CCNNNNNNNGG 1 cut(s) 285
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 3 cut(s) 471, 479, 675
AjnI CCWGG 1 cut(s) 604
AjuI GAANNNNNNNTTGG 2 cut(s) 532, 564
AluBI AGCT 1 cut(s) 402
AluI AGCT 1 cut(s) 402
Alw21I GWGCWC 1 cut(s) 365
AlwI GGATC 3 cut(s) 11, 222, 443
Ama87I CYCGRG 1 cut(s) 78
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
AspS9I GGNCC 2 cut(s) 249, 391
AsuHPI GGTGA 1 cut(s) 389
AsuII TTCGAA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 2 cut(s) 249, 391
BbsI GAAGAC 2 cut(s) 304, 540
Bbv12I GWGCWC 1 cut(s) 365
BccI CCATC 4 cut(s) 132, 152, 205, 287
BciT130I CCWGG 1 cut(s) 606
BciVI GTATCC 1 cut(s) 251
BfaI CTAG 1 cut(s) 697
BfuI GTATCC 1 cut(s) 251
Bme1390I CCNGG 1 cut(s) 606
Bme18I GGWCC 2 cut(s) 249, 391
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 2 cut(s) 249, 391
BmiI GGNNCC 2 cut(s) 250, 267
BmrFI CCNGG 1 cut(s) 606
BpiI GAAGAC 2 cut(s) 304, 540
BplI GAGNNNNNCTC 2 cut(s) 72, 104
BpmI CTGGAG 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 115
BpuEI CTTGAG 2 cut(s) 207, 356
BsaBI GATNNNNATC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 1 cut(s) 285
Bse3DI GCAATG 2 cut(s) 72, 596
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 3 cut(s) 120, 279, 313
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 285
BseMI GCAATG 2 cut(s) 72, 596
BseMII CTCAG 1 cut(s) 412
BseYI CCCAGC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 365
BsiHKCI CYCGRG 1 cut(s) 78
BslFI GGGAC 2 cut(s) 59, 262
BslI CCNNNNNNNGG 1 cut(s) 285
BsmFI GGGAC 2 cut(s) 59, 262
BsoBI CYCGRG 1 cut(s) 78
Bsp119I TTCGAA 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 3 cut(s) 16, 214, 448
Bsp19I CCATGG 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 411
BspLI GGNNCC 2 cut(s) 250, 267
BspPI GGATC 3 cut(s) 11, 222, 443
BspT104I TTCGAA 1 cut(s) 115
BsrDI GCAATG 2 cut(s) 72, 596
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 3 cut(s) 16, 214, 448
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 1 cut(s) 381
BstBI TTCGAA 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 398
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 3 cut(s) 120, 279, 313
BstKTI GATC 3 cut(s) 19, 217, 451
BstMBI GATC 3 cut(s) 16, 214, 448
BstMWI GCNNNNNNNGC 2 cut(s) 510, 598
BstNI CCWGG 1 cut(s) 606
BstSCI CCNGG 1 cut(s) 604
BstV2I GAAGAC 2 cut(s) 304, 540
BstX2I RGATCY 2 cut(s) 16, 448
BstYI RGATCY 2 cut(s) 16, 448
BsuI GTATCC 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 3 cut(s) 120, 279, 313
Cfr13I GGNCC 2 cut(s) 249, 391
CseI GACGC 1 cut(s) 502
CviAII CATG 6 cut(s) 98, 193, 262, 284, 408, 562
CviJI RGCY 4 cut(s) 266, 402, 504, 671
CviKI_1 RGCY 4 cut(s) 266, 402, 504, 671
DdeI CTNAG 1 cut(s) 398
DpnI GATC 3 cut(s) 18, 216, 450
DpnII GATC 3 cut(s) 16, 214, 448
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 249, 391
Eco57I CTGAAG 2 cut(s) 298, 534
Eco88I CYCGRG 1 cut(s) 78
EcoO109I RGGNCCY 2 cut(s) 249, 391
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 6 cut(s) 101, 196, 265, 287, 411, 565
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 59, 262
FatI CATG 6 cut(s) 97, 192, 261, 283, 407, 561
FokI GGATG 3 cut(s) 107, 266, 320
FspBI CTAG 1 cut(s) 697
GsaI CCCAGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 252
HgaI GACGC 1 cut(s) 502
Hin1II CATG 6 cut(s) 101, 196, 265, 287, 411, 565
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HinfI GANTC 9 cut(s) 85, 104, 117, 169, 240, 575, 632, 656, 682
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 5 cut(s) 210, 307, 372, 528, 637
Hpy188III TCNNGA 5 cut(s) 108, 128, 269, 335, 660
Hpy8I GTNNAC 1 cut(s) 459
HpyAV CCTTC 1 cut(s) 404
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 3 cut(s) 182, 407, 619
HpyF10VI GCNNNNNNNGC 2 cut(s) 510, 598
HpyF3I CTNAG 1 cut(s) 398
HpySE526I ACGT 1 cut(s) 52
Hsp92II CATG 6 cut(s) 101, 196, 265, 287, 411, 565
Kzo9I GATC 3 cut(s) 16, 214, 448
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 4 cut(s) 154, 282, 591, 618
MaeI CTAG 1 cut(s) 697
MaeII ACGT 1 cut(s) 52
MaeIII GTNAC 3 cut(s) 53, 128, 580
MalI GATC 3 cut(s) 18, 216, 450
MboI GATC 3 cut(s) 16, 214, 448
MboII GAAGA 4 cut(s) 113, 304, 398, 540
MflI RGATCY 2 cut(s) 16, 448
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 4 cut(s) 5, 60, 177, 675
MlyI GAGTC 2 cut(s) 249, 665
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 6 cut(s) 62, 74, 104, 382, 418, 661
Mph1103I ATGCAT 1 cut(s) 409
MseI TTAA 2 cut(s) 135, 567
MspR9I CCNGG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 606
MwoI GCNNNNNNNGC 2 cut(s) 510, 598
NcoI CCATGG 1 cut(s) 283
NdeII GATC 3 cut(s) 16, 214, 448
NlaIII CATG 6 cut(s) 101, 196, 265, 287, 411, 565
NlaIV GGNNCC 2 cut(s) 250, 267
NmuCI GTSAC 1 cut(s) 128
NsiI ATGCAT 1 cut(s) 409
NspV TTCGAA 1 cut(s) 115
PaeR7I CTCGAG 1 cut(s) 78
PfeI GAWTC 7 cut(s) 85, 104, 117, 169, 575, 632, 682
PflFI GACNNNGTC 1 cut(s) 655
PleI GAGTC 2 cut(s) 248, 664
PpsI GAGTC 2 cut(s) 248, 664
PpuMI RGGWCCY 2 cut(s) 249, 391
Psp5II RGGWCCY 2 cut(s) 249, 391
Psp6I CCWGG 1 cut(s) 604
PspFI CCCAGC 1 cut(s) 140
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 2 cut(s) 250, 267
PspPI GGNCC 2 cut(s) 249, 391
PspPPI RGGWCCY 2 cut(s) 249, 391
PspXI VCTCGAGB 1 cut(s) 78
PsuI RGATCY 2 cut(s) 16, 448
PsyI GACNNNGTC 1 cut(s) 655
SaqAI TTAA 2 cut(s) 135, 567
Sau3AI GATC 3 cut(s) 16, 214, 448
Sau96I GGNCC 2 cut(s) 249, 391
SchI GAGTC 2 cut(s) 249, 665
ScrFI CCNGG 1 cut(s) 606
SduI GDGCHC 1 cut(s) 365
SetI ASST 7 cut(s) 55, 96, 135, 254, 396, 404, 607
Sfr274I CTCGAG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 115
SinI GGWCC 2 cut(s) 249, 391
SlaI CTCGAG 1 cut(s) 78
SmlI CTYRAG 3 cut(s) 78, 222, 335
SmoI CTYRAG 3 cut(s) 78, 222, 335
Sse9I AATT 4 cut(s) 5, 60, 177, 675
SspI AATATT 1 cut(s) 148
SspMI CTAG 1 cut(s) 697
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 55
TaqI TCGA 3 cut(s) 79, 109, 115
TasI AATT 4 cut(s) 5, 60, 177, 675
TfiI GAWTC 7 cut(s) 85, 104, 117, 169, 575, 632, 682
Tru1I TTAA 2 cut(s) 135, 567
Tru9I TTAA 2 cut(s) 135, 567
TseFI GTSAC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 5 cut(s) 114, 182, 399, 425, 645
Tth111I GACNNNGTC 1 cut(s) 655
VpaK11BI GGWCC 2 cut(s) 249, 391
XhoI CTCGAG 1 cut(s) 78
XspI CTAG 1 cut(s) 697
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.