RLG00000032295

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010060
Physical Location & Seq
Forward (+)
13233653 .. 13242605
8953 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032295

Sequence Viewer

Length: 4965 bp
ATGGTCGCTTCAACAGCTTCGTCCAACTCCAATAAACCCACAATGGCGGCGAAAGTGCCGGTGGAGCCTGAGAAGGCCAATCCCTGCTGCGCTGCGTGGCAAAAGAAGTATTTGAAGAGTGAGAAGCTACGGAATCATCTGCGGGAAGCTGTGAATATCCTTCAACAGGGGACAGAAGAAGTTCAAGCACAGAATTCCAATCTCAAGAAAGAATATGAGCAGTTGCGAGTGCTGGCAGATATTGAGAAAGAGGAGAAAGTGAAAGAATATGCGGCAAGGGTCTCTCTGGAGAACGAGGTTTCTGAGTTGAAATCTGAGATACTTTCTCTCAAGCAGCGAGAAGGTGCAGATTCTCAGGATAAGAATGAAGTAAAGCTTCTTAAAGGTCAGGTTTCTGATCGGGAACAGGAAATTAGTCGTCTCAAGGAGCTGATTGAGAGAGAGAAGAAAAGAGCAGAATCAGAAAGTAAGAAAGCAGAAGTGGAGAAGAAAAAGGCTGCCGAAGCACAAAAAAGTATGAAAATTGAAAAAAAGAAGGCTGATGAAGAAAGGAAGCGTGCCAATATTGAAGGAGAGAAGGCGGAGAATTATAGGCTTCAATTGGAGGTATTGGAGAAAGACTCTGCTAAAGCAAAATCTCATTCTGAAGGGGAAAAAGAGATTAGTTGTCTCAAGGAGCTTATTGAGAGAGAGAAGAAAAGAGCAGAATCAGAAAGTAAGAAAGCAGAAGCGGAGAAGAAAAAGGCTGCTGAAGCACTGAAAATTATGAAAATTGAAAAAAGTAAGGCTGACGAAGAAAGGAAGCGTGCCATTATTGAAGGAGAGAAGGCTGAGAATTATAGGCTTCGGTTGGAGGTATTGCAAATAGAAGCTGATAAAGCGAAATCTAATTTGGCTTCAGAAACATTGAAACTCGAAGTAGCAAGCAAAAAGCTTGAAGCAGAAAAACTGAAGGTCGTCAAGGAGAGAGAATGTGCAAATTCAGGGATGGCGAAAGCTGAGGAACAGAGGAAGCTTGCAGAAGCCAACAGGAAAAAGACCATTGAAGAAAAATCTCGTGCTGAAAGTTTGTCTCTGCAATTGGTAGAGAGCAGAAAGAGGGTTGATGAACTACAAAAAGAGCTAAATGAACTTAGGTGTTCAAAAGAATTGCATGTGGATCCTGGTGGCCAACCTGACAGTAATATAATTGCTGTCGCTTCCAAAGAGATTTCAAATTTTGATGAAGCAAACAAAAGATACGAAGTTGAAAAGCAGAAGGTGGTGAAGGAGAAAAAGCAACCAGACTCAGAGATGGTGAGAACTCAAAAGCAGAAACAGCTTGCTGAAGTAAATTGGAAGAGGGCCATGGCAGAAAAATCTCGTGCTGATTATCTCTCTCGGCAGTTAGAGGAAGCTAAAACAAAGATTGAGGAATTATCTACTAGAAATTTAATTGAGGCATCTGCTGTTCAACAACTTGCCAAAGGTATGAGTGATGAAAGTGCAAAGGTGAAACTTTTAAAGAAGCAGCTGAAGTTTGAGAAAATGCAAAGAAAGCATGCTAAGGAAGTGGTCAAGGTCAAGCTGGAAAGAAATCGCAACAGTATCCTACAACAAGAAATTGGTCGCTTGAAGTTGGACTTTGATCAAATTTCACAGCGCATGGATATGCTTAATAACTCTTTCTCCTATAATGCCGAAGGTATAGATGACCATGAAAAGACCTGGAACATCATGAAAAAGCAACGGTTAATCACCAAGAATCACCAAGGGTTAGAATCTTTGCAGACGAATCTTCAAAGTGGAACTGACTTTCTGAAATCTGGCTTCCCAGTTTTGGATGAATCAGATCCTTTCAGGGAAGCCATGCAACATACTGGACCTTATCCTCCTTTATCTGGAGGAAACTGCACTGGATCTATTTCAGGTATTGATTCTAAATTAGAGTCTCCTAGAGGCTCTAAGAGAAACATGTTACACAGTTCTGCAATAAATTCAAGTACAGCATCTTTTTCTGATGGACAGTTGGTGGGCTCACAGGACAAAGGTGCCTTTTCTGTTACTGTGCCAACAAAATTAGTTGAGGAAAATGTGCAACCAACAATATCCAACTTGTCTACTGAGGTTATTAAAAGAAAGTGCAACGAAACTATTGCTGTGGTGGCTGAAAACACTGTCAAAAGTCCTGTACGAACTGATGGTATTGGACAAGTCAATGAGTGTAGCAGGAAGAGAAAGAGGATAATAGATGCAGTTGAGTCAATTGAAAATCTTTACTGTGAGGGTAAGAAGTTCCACCAGCAGGTAGATGAAAAGCTATCTGATTTGCATTGTTTGTTAAGCAAGAAAATAAAGAAACCGGTTGAAGAAAGACTGCATGAGAAGAGAAAGGCAGCTTGTGAAGGGACATTGGTATTGGCAAGTTGTGTAGTCAGGAATGAGCAAAAGAAGGCAGATAGATTCAAAAATGAATGTGCGAATGTGTGCAGACAAGTTCCGAAAATTGCGGGTGAGCTGATAGGGACTGCCCAAGCGAGCAGAGAAGGCATAAGTGATCTCGATACTAGTGATATAGCAAGTTTTGATGAAGTAACCAATGGGAACTACCTTAAACTGTTAGAATTGGACAATGCTGCTGATGAAGAGTGCTACAGGATGGCAATGGAAATGCCATTGTCTCCTACACTTCCCGAGATTGAGGTCCTAGATGTTGAAACTTCTATTGCCAATAAGGTCAATCCTTTAATAAAGGAAATTATCTGTGACAGATTTTCAAATAAGAATGGAGGTCATGGTGATTGTTGTGACACACAAGACCTGAATGGGAATGGTTCTGGTAATTCTGTAGCAATGGGTAAACCTTGGGATATCCAGGTGCAGGATGCTGGGGCTGAAGTGGTGTCTAATGCACCTACTTTAAAAAGTAAGGCTATGTTCCCATTTGGAAGTGATGGTGCTGCGGATGACAATATTCTCAATTATCATGTTGTGTTTTCAAACACTGAAGACAGCAGTAGCATTTCTAGGATATGCAACGCATCCAGAACTTGTATTGCACGGTGCTCTTTGGCTATTCATACAGAGTGGATGGTGCGAGATATTCTGTTTGCTGTTAAGAGTGAAGAAAAACTTCTCCCCAAAGAGAAGGTTTGTGTATTCTTCTCGTTGTTGCTGCTTAACTTCTCCGTTGCTTCTTCAAGTAAATTTGGGAGCATGTATTGGGAGTCCAACCCCTGCTTGGATTCTTTTGCTCGACACATGTGCTCAGTGATGTCTGATGGTGATGGCAGAAGGATATTTTCAGAATTTGGTTGTTTGGATGAATCCCTTGGTCTCATAGAGGATTTCATAATACATGGAAGAGTTTTTTATTGTAAGGATGCACCTTCCAAGACGATGGTTGGGTGTCACTCTAGCAGCTTCCTACTTGAAGATATATCCCCTCGTCCATCATCAGCGGATGAGTTGGTGGCGGGGAGCATTGTATTGGCATCACTATGTGCAGCATTTGATCACATTGGTTTTATTTGTGAAACTTCCTACACCATTTTGCATATTAGGAGATTAGATAGTTCCTTGGTTCTAAAGGTGCTGCATGTTTTTGCTTACTTGGGTGGGGAGAAGTTTTTCAACTTTAGCAACTATAATTTAGTGACTGTCATGAAATCTATTGTTACATTTCTCGAGGGAATAAGATCATCAAATTCTTCTGGTTCTTGTATTACATCAGTAAGCAATTCAGGAACTGAATTCTGTCCGTGTGTCAAATGCCCATTTTCGGAGGATGCTGTTTCTGTAGATACTGCTATATCATTGCTTTTAGAAATGCTCCAAAGTGATGCTTTCTTGGGACCTATGAGTCAGGATGTGGTGCAATCAGTCAATTCAAATACAAATATCACACTGTGCAACCTCTGTGATCTCCTATCGTTGGTGGAGCTGGTTGCGTCCAACATGAGCTGGACATGGACCATTGTCGAAGTCGTTCCTCAGCTGATGAAAATTTTGGAATCATGCAATGCTGAGAATGTTATTGCTGGAATTGTTGTCCTCCTTGGTCAACTTGGGAGGCTTGGAGTTGATTCTGTTGGCTATGAAGATAAAGGAGTGGAATTCTTGAGGCATGAGTTGTCTGCTTTTTTAGGTCGTGACATTGGGACCAATGCAGGTCTTCCTACTCAAATTGCCACCGTCACATCTATGCTGGGTCTGATGTCTTTTGATTTCAAAACAATTATCCAGAGTAACGTGAATCTTTCCGTCATTTCAAGTCAATCTGATGCGGCCCAGCACAAAGCAGGACATGGATTTTCTAGGGAAGATGAAGATGATGATAAGAAGCTCAGCAGTCGTAATGTCAGCCATGGATCCCATTTGGTGAGTGGACAAATGGACCATGGAATGGAAGATTATCTTGTGGCAGAAACTAGAAAAGTAAACGGCCATCAGTTGGGTTTATATGCAATCTTTGATGGCCATTCAGGTCAACAAGTTGCAGAATACTTGCAAGCTCATCTATTTGATAAAATACTAAGTCAGAGAGCTTATAAAGCTACAGATGATGAGATTTTGGAAAAGGTGGTTGGTTCAAGAGGCGGATCTACAGCTGATACATTGACTGTAGATCACGACCCGCAGAAGGAGAAGGATCTTGTTGAAAGCAGAGGTGGTTGTGTTGTTAAGCTGCCAGGAAATGTTCCACGTGTGGATGTACAACTCGCAATGTCGCGGGCATTTGGCGATGGTGACTTACACATAACTTCTGAACTAGATATCAGCATCGAGAAAATCGACACTGAAGTAGACTTCATTATATTGGCGAGTGATGTTTATCTGATCATGAAGGTGATGTTGAACCAAGATGCTTATGATTGCATCAGAGGTATAGATGATGCTCAAGAAGCAGCAGAGAAGTTGACTCAAAAAGCATTATCCAGGAAAAGCTACGACGATATCTTTTGCATAGTTGTGGTCTTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1655

Amino Acids

184.08

Weight (kDa)

5.83

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 1524 - 1646 9.5e-14 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34780 AT2G34780 AT2G34780 AT2G34780
fragaria_vesca FvH4_3g10781 FvH4_3g10781
malus_domestica MD05G1268800.v1.1 MD10G1247500.v1.1
prunus_persica Prupe.4G095800_v2.0.a1
pyrus_communis pycom05g25180 pycom10g20530
rosa_chinensis RchiOBHm_Chr5g0017511
rosa_laevigata RLG00000032295
rosa_multiflora Rmu_sc0008764.1_g000003
rosa_roxburghii Rroxscaffold_1G00059920
rosa_rugosa Rorug05G0036700
rosa_samantha Rh5AG130800 Rh5BG128700 Rh5CG141000 Rh5DG130900
rosa_wichuraiana Rw5G011280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4530
AasI GACNNNNNNGTC 1 cut(s) 3840
Acc36I ACCTGC 2 cut(s) 2275, 4139
AccB1I GGYRCC 1 cut(s) 2030
AccB7I CCANNNNNTGG 2 cut(s) 4384, 4432
AccI GTMKAC 2 cut(s) 2099, 4785
AccII CGCG 1 cut(s) 4711
AclWI GGATC 8 cut(s) 1154, 1167, 1826, 1906, 4344, 4357, 4588, 4638
AcoI YGGCCR 3 cut(s) 1168, 4423, 4456
AcuI CTGAAG 9 cut(s) 666, 771, 882, 971, 1347, 1535, 2895, 3006, 4800
AcvI CACGTG 1 cut(s) 4685
AdeI CACNNNGTG 2 cut(s) 3482, 3888
AfaI GTAC 3 cut(s) 1984, 2172, 4695
AflIII ACRYGT 3 cut(s) 1953, 3240, 4684
AgeI ACCGGT 1 cut(s) 2341
AhlI ACTAGT 1 cut(s) 2546
AjnI CCWGG 5 cut(s) 1162, 1706, 2853, 4669, 4916
AjuI GAANNNNNNNTTGG 4 cut(s) 875, 907, 4548, 4580
Alw21I GWGCWC 2 cut(s) 3047, 3248
Alw26I GTCTC 7 cut(s) 286, 425, 674, 1077, 1935, 2664, 3320
AlwI GGATC 8 cut(s) 1154, 1167, 1826, 1906, 4344, 4357, 4588, 4638
AlwNI CAGNNNCTG 1 cut(s) 3728
Ama87I CYCGRG 2 cut(s) 2672, 3665
AoxI GGCC 6 cut(s) 75, 1168, 1344, 4266, 4423, 4456
AsiGI ACCGGT 1 cut(s) 2341
Asp700I GAANNNNTTC 4 cut(s) 180, 3111, 3608, 3966
AspLEI GCGC 2 cut(s) 92, 1644
AspS9I GGNCC 8 cut(s) 1344, 1862, 2683, 3833, 3951, 4140, 4267, 4375
AvaI CYCGRG 2 cut(s) 2672, 3665
AvaII GGWCC 6 cut(s) 1862, 2683, 3833, 3951, 4140, 4375
BalI TGGCCA 2 cut(s) 1170, 4458
BamHI GGATCC 2 cut(s) 1159, 4349
BanI GGYRCC 1 cut(s) 2030
BanII GRGCYC 1 cut(s) 2018
BauI CACGAG 2 cut(s) 1056, 1362
BbrPI CACGTG 1 cut(s) 4685
BbsI GAAGAC 2 cut(s) 2994, 4145
Bbv12I GWGCWC 2 cut(s) 3047, 3248
BbvCI CCTCAGC 2 cut(s) 999, 3972
BceAI ACGGC 1 cut(s) 4438
BcgI CGANNNNNNTGC 4 cut(s) 2117, 2151, 3225, 3259
BciT130I CCWGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
BciVI GTATCC 1 cut(s) 1598
BclI TGATCA 3 cut(s) 1627, 3493, 4818
BcoDI GTCTC 7 cut(s) 286, 425, 674, 1077, 1935, 2664, 3320
BcuI ACTAGT 1 cut(s) 2546
BfmI CTRYAG 6 cut(s) 2632, 2826, 3777, 4536, 4584, 4602
BfuAI ACCTGC 2 cut(s) 2275, 4139
BfuI GTATCC 1 cut(s) 1598
BlpI GCTNAGC 1 cut(s) 4325
Bme1390I CCNGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
Bme18I GGWCC 6 cut(s) 1862, 2683, 3833, 3951, 4140, 4375
BmeT110I CYCGRG 2 cut(s) 2672, 3665
BmgT120I GGNCC 8 cut(s) 1344, 1862, 2683, 3833, 3951, 4140, 4267, 4375
BmiI GGNNCC 6 cut(s) 66, 1161, 2032, 3834, 4141, 4351
BmrFI CCNGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
BmrI ACTGGG 1 cut(s) 1808
BmuI ACTGGG 1 cut(s) 1808
BoxI GACNNNNGTC 1 cut(s) 3956
BpiI GAAGAC 2 cut(s) 2994, 4145
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpmI CTGGAG 2 cut(s) 308, 1902
Bpu10I CCTNAGC 3 cut(s) 999, 1545, 3972
Bpu1102I GCTNAGC 1 cut(s) 4325
BpuEI CTTGAG 6 cut(s) 188, 314, 407, 656, 4120, 4863
BsaAI YACGTR 1 cut(s) 4685
BsaBI GATNNNNATC 1 cut(s) 4812
BsaI GGTCTC 2 cut(s) 286, 3320
BsaJI CCNNGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
BsaWI WCCGGW 1 cut(s) 2341
BsaXI ACNNNNNCTCC 2 cut(s) 1652, 1682
Bse118I RCCGGY 2 cut(s) 58, 2341
Bse1I ACTGG 3 cut(s) 1814, 1864, 1900
Bse3DI GCAATG 5 cut(s) 2649, 2838, 3794, 4006, 4710
Bse8I GATNNNNATC 1 cut(s) 4812
BseBI CCWGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
BseDI CCNNGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
BseJI GATNNNNATC 1 cut(s) 4812
BseMI GCAATG 5 cut(s) 2649, 2838, 3794, 4006, 4710
BseNI ACTGG 3 cut(s) 1814, 1864, 1900
BseRI GAGGAG 1 cut(s) 266
BseYI CCCAGC 3 cut(s) 2867, 4186, 4269
BsgI GTGCAG 5 cut(s) 366, 1876, 2488, 2879, 3504
Bsh1236I CGCG 1 cut(s) 4711
BshFI GGCC 6 cut(s) 77, 1170, 1346, 4268, 4425, 4458
BshNI GGYRCC 1 cut(s) 2030
BshTI ACCGGT 1 cut(s) 2341
BsiHKAI GWGCWC 2 cut(s) 3047, 3248
BsiHKCI CYCGRG 2 cut(s) 2672, 3665
BsiSI CCGG 2 cut(s) 59, 2342
BslFI GGGAC 5 cut(s) 184, 2401, 2518, 3846, 4153
BsmAI GTCTC 7 cut(s) 286, 425, 674, 1077, 1935, 2664, 3320
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 5 cut(s) 184, 2401, 2518, 3846, 4153
BsnI GGCC 6 cut(s) 77, 1170, 1346, 4268, 4425, 4458
Bso31I GGTCTC 2 cut(s) 286, 3320
BsoBI CYCGRG 2 cut(s) 2672, 3665
Bsp1286I GDGCHC 3 cut(s) 2018, 3047, 3248
Bsp1407I TGTACA 1 cut(s) 4693
Bsp1720I GCTNAGC 1 cut(s) 4325
Bsp19I CCATGG 3 cut(s) 1347, 4345, 4378
BspANI GGCC 6 cut(s) 77, 1170, 1346, 4268, 4425, 4458
BspFNI CGCG 1 cut(s) 4711
BspHI TCATGA 3 cut(s) 1716, 3642, 4821
BspLI GGNNCC 6 cut(s) 66, 1161, 2032, 3834, 4141, 4351
BspMI ACCTGC 2 cut(s) 2275, 4139
BspPI GGATC 8 cut(s) 1154, 1167, 1826, 1906, 4344, 4357, 4588, 4638
BspT107I GGYRCC 1 cut(s) 2030
BspTNI GGTCTC 2 cut(s) 286, 3320
BsrDI GCAATG 5 cut(s) 2649, 2838, 3794, 4006, 4710
BsrFI RCCGGY 2 cut(s) 58, 2341
BsrGI TGTACA 1 cut(s) 4693
BsrI ACTGG 3 cut(s) 1814, 1864, 1900
BssAI RCCGGY 2 cut(s) 58, 2341
BssECI CCNNGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
BssSI CACGAG 2 cut(s) 1056, 1362
BssT1I CCWWGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
Bst2BI CACGAG 2 cut(s) 1056, 1362
Bst2UI CCWGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
Bst6I CTCTTC 6 cut(s) 110, 1334, 2207, 2360, 2619, 3337
BstAUI TGTACA 1 cut(s) 4693
BstBAI YACGTR 1 cut(s) 4685
BstDSI CCRYGG 3 cut(s) 1347, 4345, 4378
BstENI CCTNNNNNAGG 1 cut(s) 164
BstFNI CGCG 1 cut(s) 4711
BstHHI GCGC 2 cut(s) 92, 1644
BstMAI GTCTC 7 cut(s) 286, 425, 674, 1077, 1935, 2664, 3320
BstNI CCWGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
BstNSI RCATGY 6 cut(s) 1157, 1544, 1957, 3199, 3244, 3581
BstPAI GACNNNNGTC 1 cut(s) 3956
BstSCI CCNGG 5 cut(s) 1162, 1706, 2853, 4669, 4916
BstSFI CTRYAG 6 cut(s) 2632, 2826, 3777, 4536, 4584, 4602
BstUI CGCG 1 cut(s) 4711
BstV2I GAAGAC 2 cut(s) 2994, 4145
BstX2I RGATCY 6 cut(s) 1159, 1831, 1898, 4349, 4580, 4630
BstXI CCANNNNNNTGG 1 cut(s) 3379
BstYI RGATCY 6 cut(s) 1159, 1831, 1898, 4349, 4580, 4630
BsuI GTATCC 1 cut(s) 1598
BsuRI GGCC 6 cut(s) 77, 1170, 1346, 4268, 4425, 4458
BtgI CCRYGG 3 cut(s) 1347, 4345, 4378
BtgZI GCGATG 1 cut(s) 4737
BtsIMutI CAGTG 7 cut(s) 755, 1893, 2154, 2982, 3255, 3884, 4776
BveI ACCTGC 2 cut(s) 2275, 4139
CaiI CAGNNNCTG 1 cut(s) 3728
CciI TCATGA 3 cut(s) 1716, 3642, 4821
CfoI GCGC 2 cut(s) 92, 1644
Cfr10I RCCGGY 2 cut(s) 58, 2341
Cfr13I GGNCC 8 cut(s) 1344, 1862, 2683, 3833, 3951, 4140, 4267, 4375
CseI GACGC 1 cut(s) 3918
Csp6I GTAC 3 cut(s) 1983, 2171, 4694
CspAI ACCGGT 1 cut(s) 2341
CviQI GTAC 3 cut(s) 1983, 2171, 4694
DraI TTTAAA 2 cut(s) 1503, 2901
DraIII CACNNNGTG 2 cut(s) 3482, 3888
DrdI GACNNNNNNGTC 1 cut(s) 3840
DseDI GACNNNNNNGTC 1 cut(s) 3840
EaeI YGGCCR 3 cut(s) 1168, 4423, 4456
Eam1104I CTCTTC 6 cut(s) 110, 1334, 2207, 2360, 2619, 3337
EarI CTCTTC 6 cut(s) 110, 1334, 2207, 2360, 2619, 3337
EciI GGCGGA 2 cut(s) 596, 4593
Eco130I CCWWGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
Eco24I GRGCYC 1 cut(s) 2018
Eco31I GGTCTC 2 cut(s) 286, 3320
Eco32I GATATC 3 cut(s) 2851, 4756, 4936
Eco47I GGWCC 6 cut(s) 1862, 2683, 3833, 3951, 4140, 4375
Eco57I CTGAAG 9 cut(s) 666, 771, 882, 971, 1347, 1535, 2895, 3006, 4800
Eco72I CACGTG 1 cut(s) 4685
Eco88I CYCGRG 2 cut(s) 2672, 3665
EcoNI CCTNNNNNAGG 1 cut(s) 164
EcoO109I RGGNCCY 2 cut(s) 2683, 3833
EcoRI GAATTC 3 cut(s) 193, 3731, 4094
EcoRII CCWGG 5 cut(s) 1162, 1706, 2853, 4669, 4916
EcoRV GATATC 3 cut(s) 2851, 4756, 4936
EcoT14I CCWWGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
EcoT38I GRGCYC 1 cut(s) 2018
ErhI CCWWGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
Esp3I CGTCTC 1 cut(s) 425
FaqI GGGAC 5 cut(s) 184, 2401, 2518, 3846, 4153
FauI CCCGC 5 cut(s) 135, 2482, 3448, 4623, 4704
FbaI TGATCA 3 cut(s) 1627, 3493, 4818
FblI GTMKAC 2 cut(s) 2099, 4785
FriOI GRGCYC 1 cut(s) 2018
GlaI GCGC 2 cut(s) 91, 1643
GsaI CCCAGC 3 cut(s) 2871, 4190, 4273
GsuI CTGGAG 2 cut(s) 308, 1902
HaeIII GGCC 6 cut(s) 77, 1170, 1346, 4268, 4425, 4458
HapII CCGG 2 cut(s) 59, 2342
HgaI GACGC 1 cut(s) 3918
HhaI GCGC 2 cut(s) 92, 1644
Hin6I GCGC 2 cut(s) 90, 1642
HinP1I GCGC 2 cut(s) 90, 1642
HincII GTYRAC 3 cut(s) 4043, 4469, 4899
HindII GTYRAC 3 cut(s) 4043, 4469, 4899
HindIII AAGCTT 3 cut(s) 374, 932, 1013
HpaII CCGG 2 cut(s) 59, 2342
Hpy166II GTNNAC 8 cut(s) 2100, 2840, 4043, 4367, 4420, 4469, 4786, 4899
Hpy8I GTNNAC 8 cut(s) 2100, 2840, 4043, 4367, 4420, 4469, 4786, 4899
Hpy99I CGWCG 1 cut(s) 4934
HpyCH4IV ACGT 2 cut(s) 4230, 4684
HpySE526I ACGT 2 cut(s) 4230, 4684
HspAI GCGC 2 cut(s) 90, 1642
Ksp22I TGATCA 3 cut(s) 1627, 3493, 4818
LmnI GCTCC 7 cut(s) 64, 427, 676, 3192, 3459, 3816, 3919
MaeII ACGT 2 cut(s) 4230, 4684
MfeI CAATTG 3 cut(s) 599, 1079, 2244
MflI RGATCY 6 cut(s) 1159, 1831, 1898, 4349, 4580, 4630
MhlI GDGCHC 3 cut(s) 2018, 3047, 3248
MlsI TGGCCA 2 cut(s) 1170, 4458
MluNI TGGCCA 2 cut(s) 1170, 4458
MlyI GAGTC 7 cut(s) 614, 1280, 1937, 2249, 3215, 3850, 4894
MmeI TCCRAC 6 cut(s) 48, 831, 1599, 2115, 3234, 3957
Mox20I TGGCCA 2 cut(s) 1170, 4458
MroXI GAANNNNTTC 4 cut(s) 180, 3111, 3608, 3966
MscI TGGCCA 2 cut(s) 1170, 4458
MslI CAYNNNNRTG 5 cut(s) 1649, 3478, 4181, 4826, 4949
Msp20I TGGCCA 2 cut(s) 1170, 4458
MspA1I CMGCKG 4 cut(s) 1513, 3440, 3976, 4589
MspI CCGG 2 cut(s) 59, 2342
MspR9I CCNGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
MunI CAATTG 3 cut(s) 599, 1079, 2244
MvaI CCWGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
MvnI CGCG 1 cut(s) 4711
NcoI CCATGG 3 cut(s) 1347, 4345, 4378
NlaIV GGNNCC 6 cut(s) 66, 1161, 2032, 3834, 4141, 4351
NmeAIII GCCGAG 1 cut(s) 1360
NmuCI GTSAC 7 cut(s) 2744, 2786, 3389, 3634, 4130, 4174, 4727
NspI RCATGY 6 cut(s) 1157, 1544, 1957, 3199, 3244, 3581
PaeI GCATGC 1 cut(s) 1544
PaeR7I CTCGAG 1 cut(s) 3665
PagI TCATGA 3 cut(s) 1716, 3642, 4821
PciI ACATGT 2 cut(s) 1953, 3240
PcsI WCGNNNNNNNCGW 1 cut(s) 4770
PdmI GAANNNNTTC 4 cut(s) 180, 3111, 3608, 3966
PflMI CCANNNNNTGG 2 cut(s) 4384, 4432
PfoI TCCNGGA 1 cut(s) 4916
PinAI ACCGGT 1 cut(s) 2341
PleI GAGTC 7 cut(s) 614, 1280, 1936, 2248, 3214, 3849, 4894
PmaCI CACGTG 1 cut(s) 4685
PmlI CACGTG 1 cut(s) 4685
PpsI GAGTC 7 cut(s) 614, 1280, 1936, 2248, 3214, 3849, 4894
Ppu21I YACGTR 1 cut(s) 4685
PpuMI RGGWCCY 2 cut(s) 2683, 3833
PscI ACATGT 2 cut(s) 1953, 3240
PshAI GACNNNNGTC 1 cut(s) 3956
PsiI TTATAA 1 cut(s) 4530
Psp5II RGGWCCY 2 cut(s) 2683, 3833
Psp6I CCWGG 5 cut(s) 1162, 1706, 2853, 4669, 4916
PspCI CACGTG 1 cut(s) 4685
PspFI CCCAGC 3 cut(s) 2867, 4186, 4269
PspGI CCWGG 5 cut(s) 1162, 1706, 2853, 4669, 4916
PspN4I GGNNCC 6 cut(s) 66, 1161, 2032, 3834, 4141, 4351
PspPI GGNCC 8 cut(s) 1344, 1862, 2683, 3833, 3951, 4140, 4267, 4375
PspPPI RGGWCCY 2 cut(s) 2683, 3833
PstNI CAGNNNCTG 1 cut(s) 3728
PsuI RGATCY 6 cut(s) 1159, 1831, 1898, 4349, 4580, 4630
PvuII CAGCTG 3 cut(s) 1513, 3976, 4589
RsaI GTAC 3 cut(s) 1984, 2172, 4695
RsaNI GTAC 3 cut(s) 1983, 2171, 4694
RseI CAYNNNNRTG 5 cut(s) 1649, 3478, 4181, 4826, 4949
Sau96I GGNCC 8 cut(s) 1344, 1862, 2683, 3833, 3951, 4140, 4267, 4375
SchI GAGTC 7 cut(s) 614, 1280, 1937, 2249, 3215, 3850, 4894
ScrFI CCNGG 5 cut(s) 1164, 1708, 2855, 4671, 4918
SduI GDGCHC 3 cut(s) 2018, 3047, 3248
SfcI CTRYAG 6 cut(s) 2632, 2826, 3777, 4536, 4584, 4602
Sfr274I CTCGAG 1 cut(s) 3665
SinI GGWCC 6 cut(s) 1862, 2683, 3833, 3951, 4140, 4375
SlaI CTCGAG 1 cut(s) 3665
SmiMI CAYNNNNRTG 5 cut(s) 1649, 3478, 4181, 4826, 4949
SmlI CTYRAG 7 cut(s) 203, 329, 422, 671, 3665, 4099, 4878
SmoI CTYRAG 7 cut(s) 203, 329, 422, 671, 3665, 4099, 4878
SpeI ACTAGT 1 cut(s) 2546
SphI GCATGC 1 cut(s) 1544
SspI AATATT 2 cut(s) 565, 2953
StyD4I CCNGG 5 cut(s) 1162, 1706, 2853, 4669, 4916
StyI CCWWGG 8 cut(s) 1347, 1750, 2843, 3310, 3558, 4036, 4345, 4378
TaiI ACGT 2 cut(s) 4233, 4687
TaqI TCGA 7 cut(s) 915, 2541, 3235, 3666, 3960, 4764, 4773
TatI WGTACW 2 cut(s) 1982, 4693
TauI GCSGC 3 cut(s) 50, 275, 4268
TscAI CASTG 7 cut(s) 762, 1900, 2161, 2989, 3255, 3891, 4783
TseFI GTSAC 7 cut(s) 2744, 2786, 3389, 3634, 4130, 4174, 4727
Tsp45I GTSAC 7 cut(s) 2744, 2786, 3389, 3634, 4130, 4174, 4727
TspGWI ACGGA 4 cut(s) 145, 3157, 3729, 4231
TspRI CASTG 7 cut(s) 762, 1900, 2161, 2989, 3255, 3891, 4783
Van91I CCANNNNNTGG 2 cut(s) 4384, 4432
VpaK11BI GGWCC 6 cut(s) 1862, 2683, 3833, 3951, 4140, 4375
XagI CCTNNNNNAGG 1 cut(s) 164
XceI RCATGY 6 cut(s) 1157, 1544, 1957, 3199, 3244, 3581
XcmI CCANNNNNNNNNTGG 2 cut(s) 3217, 4361
XhoI CTCGAG 1 cut(s) 3665
XmiI GTMKAC 2 cut(s) 2099, 4785
XmnI GAANNNNTTC 4 cut(s) 180, 3111, 3608, 3966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.