RLG00000032321

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018767
Physical Location & Seq
Reverse (-)
13495490 .. 13496182
693 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032321

Sequence Viewer

Length: 693 bp
ATGGAGATCTTCTCTTCCGAGACTCGACAGTGGAAGCAATTGGTTGTACAATGCCCACCAAGCTTTATATGTTTGGGTGCTGGTTCGGAGGTTGTTGCTTACAATGGAATGTTGTATTGGTTGTTAGGTGCTAATAATAATATTGTTGATTTGGATCCAATGTTCAGTAGTGATGTTATTGATCAATGCCGTTTTGGTATCAACGGCACCGTAAATACATACATGTGTCTAGGTGTTTGCGGCGGACGTCTGCGGATGTGCCAGAGGGATTTTGTAGGTAAGCAGGATTTTGTAGGTAACCGTCACCTAAGTGTTTGGGAGTGGAAAGTAGAGGTGGATGACAATGGATGCAAGATGCAAAAATGGTGCTTGATAGTTGACCGTCTTTCCATCGACCAGCTGGTTTTAAAATATCCTTTGATCTCTGAAAATAAATTGCCGTACTGTAGGAGGTTTCAGGTGCTAAGTTTTCACCCATACAATGAGGATGCGGTGTTTTTAGAGACTGAGCACCCAAATTGCATTGCTCTGTGCAATATGCGCGAAAGAACCCTTGAGATAGTTTCAGAATTTAATCCTAAGATCAATTTGGAAAGCTGGTTTGGAAGTTGGTTTCGCTCTGCGTGTTTGGGAAGCAGAAACGTCTACCCATATGTGATCCCGTGGTTGCCTACACCTGTCCCTAAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

231

Amino Acids

26.58

Weight (kDa)

5.95

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 1 - 190 6.4e-08 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018767)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47695
rosa_chinensis RchiOBHm_Chr5g0018111
rosa_laevigata RLG00000032321 RLG00000032322
rosa_multiflora Rmu_sc0010280.1_g000022 Rmu_sc0013814.1_g000004
rosa_roxburghii Rroxscaffold_1G00069250
rosa_samantha Rh5CG143900 Rh5DG133700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 250
AccB1I GGYRCC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 645
AccII CGCG 1 cut(s) 543
AciI CCGC 4 cut(s) 240, 243, 253, 491
AclWI GGATC 3 cut(s) 149, 162, 652
AcsI RAATTY 1 cut(s) 569
AcyI GRCGYC 1 cut(s) 247
AfaI GTAC 2 cut(s) 48, 443
AflIII ACRYGT 1 cut(s) 222
AjuI GAANNNNNNNTTGG 4 cut(s) 100, 132, 585, 617
AleI CACNNNNGTG 1 cut(s) 309
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 3 cut(s) 63, 400, 597
AluI AGCT 3 cut(s) 63, 400, 597
Alw21I GWGCWC 1 cut(s) 513
Alw26I GTCTC 2 cut(s) 14, 497
AlwI GGATC 3 cut(s) 149, 162, 652
ApoI RAATTY 1 cut(s) 569
AspLEI GCGC 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 296, 464
BamHI GGATCC 1 cut(s) 154
BanI GGYRCC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 513
BccI CCATC 1 cut(s) 398
BceAI ACGGC 3 cut(s) 174, 220, 424
BclI TGATCA 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 14, 497
BfaI CTAG 2 cut(s) 230, 691
BfmI CTRYAG 1 cut(s) 445
BglII AGATCT 1 cut(s) 6
BisI GCNGC 1 cut(s) 241
BlsI GCNGC 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 156, 208
BmsI GCATC 3 cut(s) 338, 345, 478
BplI GAGNNNNNCTC 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 575
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 247
BsaJI CCNNGG 1 cut(s) 662
Bse3DI GCAATG 1 cut(s) 522
Bse8I GATNNNNATC 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 662
BseGI GGATG 4 cut(s) 261, 343, 353, 493
BseJI GATNNNNATC 1 cut(s) 153
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 1 cut(s) 498
Bsh1236I CGCG 1 cut(s) 543
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 513
BslFI GGGAC 1 cut(s) 665
BsmAI GTCTC 2 cut(s) 14, 497
BsmFI GGGAC 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 513
Bsp1407I TGTACA 1 cut(s) 46
Bsp143I GATC 6 cut(s) 6, 154, 181, 420, 582, 657
BspACI CCGC 4 cut(s) 240, 243, 253, 491
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 1 cut(s) 543
BspLI GGNNCC 2 cut(s) 156, 208
BspPI GGATC 3 cut(s) 149, 162, 652
BspT107I GGYRCC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 522
BsrGI TGTACA 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 662
BssMI GATC 6 cut(s) 6, 154, 181, 420, 582, 657
BssNI GRCGYC 1 cut(s) 247
Bst4CI ACNGT 5 cut(s) 30, 211, 302, 383, 446
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 247
BstAUI TGTACA 1 cut(s) 46
BstDEI CTNAG 4 cut(s) 308, 464, 507, 579
BstDSI CCRYGG 1 cut(s) 662
BstEII GGTNACC 1 cut(s) 296
BstF5I GGATG 4 cut(s) 261, 343, 353, 493
BstFNI CGCG 1 cut(s) 543
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 6 cut(s) 9, 157, 184, 423, 585, 660
BstMAI GTCTC 2 cut(s) 14, 497
BstMBI GATC 6 cut(s) 6, 154, 181, 420, 582, 657
BstMWI GCNNNNNNNGC 2 cut(s) 60, 540
BstNSI RCATGY 1 cut(s) 226
BstPI GGTNACC 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 445
BstUI CGCG 1 cut(s) 543
BstX2I RGATCY 2 cut(s) 6, 154
BstYI RGATCY 2 cut(s) 6, 154
BtgI CCRYGG 1 cut(s) 662
BtsCI GGATG 4 cut(s) 261, 343, 353, 493
BtsIMutI CAGTG 1 cut(s) 35
CfoI GCGC 1 cut(s) 543
Csp6I GTAC 2 cut(s) 47, 442
CviAII CATG 1 cut(s) 223
CviJI RGCY 3 cut(s) 63, 400, 597
CviKI_1 RGCY 3 cut(s) 63, 400, 597
CviQI GTAC 2 cut(s) 47, 442
DdeI CTNAG 4 cut(s) 308, 464, 507, 579
DpnI GATC 6 cut(s) 8, 156, 183, 422, 584, 659
DpnII GATC 6 cut(s) 6, 154, 181, 420, 582, 657
DraI TTTAAA 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
EciI GGCGGA 1 cut(s) 258
Eco91I GGTNACC 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 296
FaeI CATG 1 cut(s) 226
FaiI YATR 8 cut(s) 68, 70, 220, 224, 478, 539, 652, 654
FaqI GGGAC 1 cut(s) 665
FatI CATG 1 cut(s) 222
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 1 cut(s) 241
FokI GGATG 4 cut(s) 268, 350, 360, 500
Fsp4HI GCNGC 1 cut(s) 241
FspBI CTAG 2 cut(s) 230, 691
GlaI GCGC 1 cut(s) 542
GluI GCNGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 543
Hin1I GRCGYC 1 cut(s) 247
Hin1II CATG 1 cut(s) 226
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 1 cut(s) 22
HphI GGTGA 2 cut(s) 296, 464
Hpy166II GTNNAC 2 cut(s) 379, 646
Hpy188I TCNGA 4 cut(s) 19, 88, 427, 568
Hpy8I GTNNAC 2 cut(s) 379, 646
HpyCH4III ACNGT 5 cut(s) 30, 211, 302, 383, 446
HpyCH4IV ACGT 2 cut(s) 247, 642
HpyCH4V TGCA 4 cut(s) 351, 358, 522, 534
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 540
HpyF3I CTNAG 4 cut(s) 308, 464, 507, 579
HpySE526I ACGT 2 cut(s) 247, 642
Hsp92I GRCGYC 1 cut(s) 247
Hsp92II CATG 1 cut(s) 226
HspAI GCGC 1 cut(s) 541
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 6 cut(s) 6, 154, 181, 420, 582, 657
LpnPI CCDG 7 cut(s) 66, 269, 275, 386, 410, 443, 583
LweI GCATC 3 cut(s) 338, 345, 478
MaeI CTAG 2 cut(s) 230, 691
MaeII ACGT 2 cut(s) 247, 642
MaeIII GTNAC 2 cut(s) 296, 302
MalI GATC 6 cut(s) 8, 156, 183, 422, 584, 659
MboI GATC 6 cut(s) 6, 154, 181, 420, 582, 657
MboII GAAGA 1 cut(s) 6
MfeI CAATTG 1 cut(s) 38
MflI RGATCY 2 cut(s) 6, 154
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 5 cut(s) 38, 434, 517, 569, 586
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 5 cut(s) 82, 258, 325, 444, 478
MseI TTAA 2 cut(s) 407, 573
MslI CAYNNNNRTG 2 cut(s) 223, 309
MspA1I CMGCKG 1 cut(s) 400
MunI CAATTG 1 cut(s) 38
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 2 cut(s) 60, 540
NdeI CATATG 1 cut(s) 652
NdeII GATC 6 cut(s) 6, 154, 181, 420, 582, 657
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 2 cut(s) 156, 208
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 309
PciI ACATGT 1 cut(s) 222
PkrI GCNGC 1 cut(s) 242
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PscI ACATGT 1 cut(s) 222
PspEI GGTNACC 1 cut(s) 296
PspN4I GGNNCC 2 cut(s) 156, 208
PsuI RGATCY 2 cut(s) 6, 154
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 2 cut(s) 48, 443
RsaNI GTAC 2 cut(s) 47, 442
RseI CAYNNNNRTG 2 cut(s) 223, 309
SaqAI TTAA 2 cut(s) 407, 573
SatI GCNGC 1 cut(s) 241
Sau3AI GATC 6 cut(s) 6, 154, 181, 420, 582, 657
SchI GAGTC 1 cut(s) 16
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 3 cut(s) 338, 345, 478
SfcI CTRYAG 1 cut(s) 445
SmiMI CAYNNNNRTG 2 cut(s) 223, 309
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 5 cut(s) 38, 434, 517, 569, 586
SsiI CCGC 4 cut(s) 240, 243, 253, 491
SspI AATATT 1 cut(s) 142
SspMI CTAG 2 cut(s) 230, 691
TaaI ACNGT 5 cut(s) 30, 211, 302, 383, 446
TaiI ACGT 2 cut(s) 250, 645
TaqI TCGA 2 cut(s) 25, 393
TasI AATT 5 cut(s) 38, 434, 517, 569, 586
TatI WGTACW 1 cut(s) 46
TauI GCSGC 1 cut(s) 243
Tru1I TTAA 2 cut(s) 407, 573
Tru9I TTAA 2 cut(s) 407, 573
TscAI CASTG 1 cut(s) 35
TseFI GTSAC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 302
TspRI CASTG 1 cut(s) 35
XapI RAATTY 1 cut(s) 569
XceI RCATGY 1 cut(s) 226
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 645
XspI CTAG 2 cut(s) 230, 691
ZraI GACGTC 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.