RLG00000032322

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018767
Physical Location & Seq
Reverse (-)
13496215 .. 13496895
681 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032322

Sequence Viewer

Length: 681 bp
ATGAGTACCGGCATTCCCTTAAGCTTTGTGGTTAATGCGCCTGGCTTGGCCACAACTACCCGAATTGATGATCTCCCCAACTTTATATTGGCTGAAATCCTTTCTCGACTTCCTTGCAAAAAAACAGTATTTCGAGCCAAGTGTGTGTCCAGGCGTTGGTTGAGTCTCCTCTCAGACCCTTATTTTGTTAGAACCTTTTTAAGTCTCCAAGGCGTTGATGAAGGAAATGCATTTATAGGTGCAGGTATTGTTGTTGTTTTGCCCTTCCCTAAGAATAAACTCAGACCCAAACTTGAAGTCCATGCGCATCCGGTAGTCAAAACAAAAGAAAGCCATTTCTCTCTGGATTTCCTCCCATGTTTTCAGGAAGAGAAAGAACGAGAGGTTCGACTATTGGTGGCAGGAACATATAATGACTTGGTTTGTGCTGCAAAAGCTACAGAACCTACGAGGAGGATGGGGAGATTTACTCCATCCGTAATCCTTACTACATCTGTAATCCATATACCAAGCAGTGGGTTGCTCTTCCTCCGAACCATCTCAGAGTCCCCTTCGGCTGTGGAACACTTGTGGGATTCATCTGTGAACCATACTTTAGGTACTCTGCAGATGGCAAAGAAGAACAAACTAGTAGTATCACCCTTAATGCCGAGTATAGGTGGAGTATTGTGCAAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

227

Amino Acids

25.0

Weight (kDa)

9.54

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 63 2.5e-07 F-box domain
F-box-like PF12937 22 - 64 4.2e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018767)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47695
rosa_chinensis RchiOBHm_Chr5g0018111
rosa_laevigata RLG00000032321 RLG00000032322
rosa_multiflora Rmu_sc0010280.1_g000022 Rmu_sc0013814.1_g000004
rosa_roxburghii Rroxscaffold_1G00069250
rosa_samantha Rh5CG143900 Rh5DG133700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 306
Acc36I ACCTGC 1 cut(s) 233
AccB7I CCANNNNNTGG 2 cut(s) 156, 515
AcoI YGGCCR 1 cut(s) 48
AfaI GTAC 2 cut(s) 7, 601
AfiI CCNNNNNNNGG 3 cut(s) 156, 515, 656
AflII CTTAAG 1 cut(s) 19
AgsI TTSAA 1 cut(s) 296
AhlI ACTAGT 1 cut(s) 628
AjnI CCWGG 2 cut(s) 40, 149
AluBI AGCT 2 cut(s) 24, 437
AluI AGCT 2 cut(s) 24, 437
Alw26I GTCTC 2 cut(s) 170, 209
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 428
ArsI GACNNNNNNTTYG 4 cut(s) 167, 199, 282, 314
AspLEI GCGC 2 cut(s) 40, 307
AsuHPI GGTGA 1 cut(s) 630
BalI TGGCCA 1 cut(s) 50
BbvI GCAGC 1 cut(s) 415
BccI CCATC 4 cut(s) 451, 481, 545, 604
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BciT130I CCWGG 2 cut(s) 42, 151
BcoDI GTCTC 2 cut(s) 170, 209
BcuI ACTAGT 1 cut(s) 628
BfaI CTAG 1 cut(s) 629
BfmI CTRYAG 2 cut(s) 438, 605
BfrI CTTAAG 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 233
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 2 cut(s) 42, 151
BmrFI CCNGG 2 cut(s) 42, 151
BmsI GCATC 1 cut(s) 316
BplI GAGNNNNNCTC 2 cut(s) 454, 486
BsaJI CCNNGG 1 cut(s) 208
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 515, 656
Bse118I RCCGGY 1 cut(s) 8
BseBI CCWGG 2 cut(s) 42, 151
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 3 cut(s) 307, 462, 473
BseLI CCNNNNNNNGG 3 cut(s) 156, 515, 656
BseMII CTCAG 3 cut(s) 186, 295, 555
BseRI GAGGAG 2 cut(s) 158, 466
BseXI GCAGC 1 cut(s) 415
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 9, 311
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 3 cut(s) 156, 515, 656
BsmAI GTCTC 2 cut(s) 170, 209
BsmFI GGGAC 1 cut(s) 532
BsmI GAATGC 1 cut(s) 12
BsnI GGCC 1 cut(s) 50
Bsp143I GATC 1 cut(s) 70
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 3 cut(s) 185, 294, 554
BspMAI CTGCAG 1 cut(s) 609
BspMI ACCTGC 1 cut(s) 233
BspQI GCTCTTC 1 cut(s) 530
BspTI CTTAAG 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 8
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 208
Bst2UI CCWGG 2 cut(s) 42, 151
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 2 cut(s) 363, 530
BstAFI CTTAAG 1 cut(s) 19
BstDEI CTNAG 4 cut(s) 172, 270, 281, 541
BstF5I GGATG 3 cut(s) 307, 462, 473
BstHHI GCGC 2 cut(s) 40, 307
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 2 cut(s) 170, 209
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNI CCWGG 2 cut(s) 42, 151
BstSCI CCNGG 2 cut(s) 40, 149
BstSFI CTRYAG 2 cut(s) 438, 605
BstV1I GCAGC 1 cut(s) 415
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 3 cut(s) 307, 462, 473
BtsI GCAGTG 1 cut(s) 520
BtsIMutI CAGTG 1 cut(s) 520
BveI ACCTGC 1 cut(s) 233
CfoI GCGC 2 cut(s) 40, 307
Cfr10I RCCGGY 1 cut(s) 8
Csp6I GTAC 2 cut(s) 6, 600
CviAII CATG 2 cut(s) 302, 357
CviJI RGCY 8 cut(s) 24, 45, 50, 92, 137, 333, 437, 557
CviKI_1 RGCY 8 cut(s) 24, 45, 50, 92, 137, 333, 437, 557
CviQI GTAC 2 cut(s) 6, 600
DdeI CTNAG 4 cut(s) 172, 270, 281, 541
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 363, 530
EarI CTCTTC 2 cut(s) 363, 530
Eco130I CCWWGG 1 cut(s) 208
EcoRII CCWGG 2 cut(s) 40, 149
EcoT14I CCWWGG 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 2 cut(s) 305, 360
FaqI GGGAC 1 cut(s) 532
FatI CATG 2 cut(s) 301, 356
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 3 cut(s) 294, 460, 469
Fsp4HI GCNGC 1 cut(s) 429
FspAI RTGCGCAY 1 cut(s) 306
FspBI CTAG 1 cut(s) 629
FspI TGCGCA 1 cut(s) 306
GlaI GCGC 2 cut(s) 39, 306
GluI GCNGC 1 cut(s) 429
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 9, 311
HhaI GCGC 2 cut(s) 40, 307
Hin1II CATG 2 cut(s) 305, 360
Hin6I GCGC 2 cut(s) 38, 305
HinP1I GCGC 2 cut(s) 38, 305
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 3 cut(s) 163, 545, 575
HpaII CCGG 2 cut(s) 9, 311
HphI GGTGA 1 cut(s) 630
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 4 cut(s) 175, 284, 533, 544
Hpy188III TCNNGA 3 cut(s) 105, 344, 365
Hpy8I GTNNAC 1 cut(s) 586
HpyAV CCTTC 3 cut(s) 215, 274, 561
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 6 cut(s) 117, 230, 242, 431, 607, 672
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 4 cut(s) 172, 270, 281, 541
Hsp92II CATG 2 cut(s) 305, 360
HspAI GCGC 2 cut(s) 38, 305
Kzo9I GATC 1 cut(s) 70
LguI GCTCTTC 1 cut(s) 530
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 629
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 3 cut(s) 380, 517, 631
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 1 cut(s) 63
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 2 cut(s) 172, 554
MnlI CCTC 6 cut(s) 179, 362, 376, 444, 447, 539
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 232
MscI TGGCCA 1 cut(s) 50
MseI TTAA 4 cut(s) 20, 33, 200, 644
Msp20I TGGCCA 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 19
MspI CCGG 2 cut(s) 9, 311
MspR9I CCNGG 2 cut(s) 42, 151
Mva1269I GAATGC 1 cut(s) 12
MvaI CCWGG 2 cut(s) 42, 151
MwoI GCNNNNNNNGC 1 cut(s) 434
NdeII GATC 1 cut(s) 70
NlaIII CATG 2 cut(s) 305, 360
NmeAIII GCCGAG 1 cut(s) 675
NsbI TGCGCA 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 385
PctI GAATGC 1 cut(s) 12
PfeI GAWTC 1 cut(s) 575
PflMI CCANNNNNTGG 2 cut(s) 156, 515
PkrI GCNGC 1 cut(s) 430
PleI GAGTC 2 cut(s) 171, 553
PpsI GAGTC 2 cut(s) 171, 553
Psp6I CCWGG 2 cut(s) 40, 149
PspGI CCWGG 2 cut(s) 40, 149
PstI CTGCAG 1 cut(s) 609
RsaI GTAC 2 cut(s) 7, 601
RsaNI GTAC 2 cut(s) 6, 600
SapI GCTCTTC 1 cut(s) 530
SaqAI TTAA 4 cut(s) 20, 33, 200, 644
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 1 cut(s) 70
SchI GAGTC 2 cut(s) 172, 554
ScrFI CCNGG 2 cut(s) 42, 151
SetI ASST 9 cut(s) 26, 197, 241, 247, 387, 439, 448, 601, 661
SfaNI GCATC 1 cut(s) 316
SfcI CTRYAG 2 cut(s) 438, 605
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
SpeI ACTAGT 1 cut(s) 628
Sse9I AATT 1 cut(s) 63
SspMI CTAG 1 cut(s) 629
StyD4I CCNGG 2 cut(s) 40, 149
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 3 cut(s) 106, 133, 388
TasI AATT 1 cut(s) 63
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 4 cut(s) 20, 33, 200, 644
Tru9I TTAA 4 cut(s) 20, 33, 200, 644
TscAI CASTG 1 cut(s) 520
TseI GCWGC 1 cut(s) 428
TspDTI ATGAA 2 cut(s) 234, 567
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 1 cut(s) 520
Van91I CCANNNNNTGG 2 cut(s) 156, 515
Vha464I CTTAAG 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XspI CTAG 1 cut(s) 629
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.