RLG00000034297

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018614
Physical Location & Seq
Forward (+)
40215716 .. 40216797
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034297

Sequence Viewer

Length: 900 bp
ATGGCCAAGCCCCAGTTCCTCCTCCTCCTTCTCCTCGTACTCCTCCTCAACTGCGACCTAACATCCCCCTCAGTCTTCAAGATACACTCATCTGGTCACCAAAAGCTCTCTACTGATTCCACCACCATCCCTCAGCCCCCACCAGCAAAGTTACTCGTTACTCAGTCACGAGACCTATATTTCGTAGTTGAAATCAACATAGGCATACCCACCCTCAATTTCTTCAATCTGGCCCTTGATATAAAAAATCCATTCACATGGGTTGGCGCTTCCAAGCCAAACATCTCGCATTCATTCCTGGCCATGCCAGGAAAGGTCCCGATGTGCCTGCCGCCTCTGTTCGCCTCTGCTTCATCTTGCACATATGACTTTGGTTATGCTCGTGGGATTAGTAGAGATGCAGATGGCATCTTAGGGTTGGGAGTAGGGCACCCAGCCAACCTGTTAGAGGGGCTGGGGGCGGCAACTCTTTATCAATTCTCTTACTGTTTGCCAATGAACTATGTGATTGAAGAAAGTACTGACCAGCAACGCCACCTGGCTACTCTAAACTTCGGAGAAAATGCAGCTATCAGTGGTCGTCGGGCTGTGAGAACGCCCATTCTAAGGCTGAAGGGGTCAGGACCCACCAAGGATTTTTCATATCTGAATCTGACAGGGATCTATGTGAACCGCCATGGGCTGCATATAAACACACAAGTCTTTCATCCCTCCCAGGGAGGATTCATTGTGGACACAGATTCCCATAATATCTACTTGAGACTGCTGAGCCTCATGCAGCAGTGTAACAAAGTTCGGGAGCAAAAGCCAGATTTGAAGCGTGAATGTCAAAGTGGAACCTGTGTCCGACTTGCTGGAGAACACCCAAGACACTGGGAGCAGAACCACCATCATGGATAA

Protein Analysis

300

Amino Acids

33.11

Weight (kDa)

8.81

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018614)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0044761
rosa_laevigata RLG00000034297
rosa_multiflora Rmu_ssc0000442.1_g000001
rosa_roxburghii Rroxscaffold_1G00036270
rosa_rugosa Rorug05G0218000
rosa_samantha Rh5AG300800 Rh5BG307600 Rh5DG317700
rosa_wichuraiana Rw5G027830 Rw5G027870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 429
AciI CCGC 3 cut(s) 332, 461, 673
AclWI GGATC 1 cut(s) 668
AcoI YGGCCR 2 cut(s) 3, 300
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 2 cut(s) 39, 520
AfiI CCNNNNNNNGG 3 cut(s) 448, 606, 716
AgsI TTSAA 5 cut(s) 79, 191, 226, 512, 817
AjnI CCWGG 4 cut(s) 297, 307, 537, 714
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 2 cut(s) 106, 569
AluI AGCT 2 cut(s) 106, 569
Alw26I GTCTC 2 cut(s) 165, 754
AlwI GGATC 1 cut(s) 668
AoxI GGCC 3 cut(s) 3, 231, 300
ApeKI GCWGC 3 cut(s) 566, 682, 778
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 3 cut(s) 232, 316, 623
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 2 cut(s) 316, 623
BaeGI GKGCMC 1 cut(s) 432
BalI TGGCCA 2 cut(s) 5, 302
BanI GGYRCC 1 cut(s) 429
BauI CACGAG 2 cut(s) 168, 381
BbsI GAAGAC 1 cut(s) 67
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 578, 669, 790
BccI CCATC 3 cut(s) 134, 398, 897
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 4 cut(s) 299, 309, 539, 716
BcoDI GTCTC 2 cut(s) 165, 754
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 5 cut(s) 332, 462, 567, 683, 779
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 5 cut(s) 333, 463, 568, 684, 780
BmcAI AGTACT 1 cut(s) 520
Bme1390I CCNGG 4 cut(s) 299, 309, 539, 716
Bme18I GGWCC 2 cut(s) 316, 623
BmgT120I GGNCC 3 cut(s) 232, 316, 623
BmiI GGNNCC 4 cut(s) 318, 431, 625, 838
BmrFI CCNGG 4 cut(s) 299, 309, 539, 716
BmrI ACTGGG 2 cut(s) 7, 883
BmsI GCATC 2 cut(s) 388, 417
BmuI ACTGGG 2 cut(s) 7, 883
BpiI GAAGAC 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 876
Bpu10I CCTNAGC 1 cut(s) 132
Bpu1102I GCTNAGC 1 cut(s) 767
BpuEI CTTGAG 1 cut(s) 778
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 4 cut(s) 630, 676, 714, 715
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 606, 716
Bse1I ACTGG 2 cut(s) 13, 878
BseBI CCWGG 4 cut(s) 299, 309, 539, 716
BseDI CCNNGG 4 cut(s) 630, 676, 714, 715
BseGI GGATG 3 cut(s) 62, 126, 706
BseLI CCNNNNNNNGG 3 cut(s) 448, 606, 716
BseMII CTCAG 4 cut(s) 84, 146, 176, 758
BseNI ACTGG 2 cut(s) 13, 878
BseRI GAGGAG 5 cut(s) 11, 14, 23, 32, 35
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 3 cut(s) 578, 669, 790
BseYI CCCAGC 2 cut(s) 433, 454
BshFI GGCC 3 cut(s) 5, 233, 302
BshNI GGYRCC 1 cut(s) 429
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 3 cut(s) 448, 606, 716
BsmAI GTCTC 2 cut(s) 165, 754
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 3 cut(s) 5, 233, 302
Bso31I GGTCTC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 1 cut(s) 660
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 3 cut(s) 332, 461, 673
BspANI GGCC 3 cut(s) 5, 233, 302
BspCNI CTCAG 4 cut(s) 83, 145, 175, 759
BspLI GGNNCC 4 cut(s) 318, 431, 625, 838
BspPI GGATC 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 165
BsrI ACTGG 2 cut(s) 13, 878
BssECI CCNNGG 4 cut(s) 630, 676, 714, 715
BssMI GATC 1 cut(s) 660
BssSI CACGAG 2 cut(s) 168, 381
BssT1I CCWWGG 2 cut(s) 630, 676
Bst2BI CACGAG 2 cut(s) 168, 381
Bst2UI CCWGG 4 cut(s) 299, 309, 539, 716
Bst4CI ACNGT 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 6 cut(s) 70, 132, 162, 412, 605, 767
BstDSI CCRYGG 1 cut(s) 676
BstEII GGTNACC 1 cut(s) 95
BstENI CCTNNNNNAGG 1 cut(s) 446
BstF5I GGATG 3 cut(s) 62, 126, 706
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 1 cut(s) 663
BstMAI GTCTC 2 cut(s) 165, 754
BstMBI GATC 1 cut(s) 660
BstNI CCWGG 4 cut(s) 299, 309, 539, 716
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 4 cut(s) 297, 307, 537, 714
BstSLI GKGCMC 1 cut(s) 432
BstV1I GCAGC 3 cut(s) 578, 669, 790
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 660
BstXI CCANNNNNNTGG 3 cut(s) 258, 873, 893
BstYI RGATCY 1 cut(s) 660
BsuRI GGCC 3 cut(s) 5, 233, 302
BtgI CCRYGG 1 cut(s) 676
BtsCI GGATG 3 cut(s) 62, 126, 706
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 3 cut(s) 580, 788, 871
Cac8I GCNNGC 1 cut(s) 329
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 232, 316, 623
Csp6I GTAC 2 cut(s) 38, 519
CviAII CATG 5 cut(s) 258, 304, 677, 775, 893
CviQI GTAC 2 cut(s) 38, 519
DdeI CTNAG 6 cut(s) 70, 132, 162, 412, 605, 767
DpnI GATC 1 cut(s) 662
DpnII GATC 1 cut(s) 660
EaeI YGGCCR 2 cut(s) 3, 300
Eco130I CCWWGG 2 cut(s) 630, 676
Eco31I GGTCTC 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 316, 623
Eco57I CTGAAG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 95
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoO109I RGGNCCY 2 cut(s) 316, 623
EcoO65I GGTNACC 1 cut(s) 95
EcoRII CCWGG 4 cut(s) 297, 307, 537, 714
EcoT14I CCWWGG 2 cut(s) 630, 676
ErhI CCWWGG 2 cut(s) 630, 676
FaeI CATG 5 cut(s) 261, 307, 680, 778, 896
FaqI GGGAC 1 cut(s) 302
FatI CATG 5 cut(s) 257, 303, 676, 774, 892
FauNDI CATATG 1 cut(s) 364
Fnu4HI GCNGC 5 cut(s) 332, 462, 567, 683, 779
FokI GGATG 3 cut(s) 49, 113, 693
Fsp4HI GCNGC 5 cut(s) 332, 462, 567, 683, 779
GlaI GCGC 1 cut(s) 268
GluI GCNGC 5 cut(s) 332, 462, 567, 683, 779
GsaI CCCAGC 2 cut(s) 437, 458
GsuI CTGGAG 1 cut(s) 876
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 5, 233, 302
HhaI GCGC 1 cut(s) 269
Hin1II CATG 5 cut(s) 261, 307, 680, 778, 896
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HinfI GANTC 4 cut(s) 116, 649, 723, 740
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 2 cut(s) 670, 733
Hpy188I TCNGA 4 cut(s) 557, 648, 654, 848
Hpy188III TCNNGA 5 cut(s) 79, 168, 319, 621, 797
Hpy8I GTNNAC 2 cut(s) 670, 733
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 2 cut(s) 38, 607
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4V TGCA 5 cut(s) 360, 401, 566, 685, 778
HpyF3I CTNAG 6 cut(s) 70, 132, 162, 412, 605, 767
Hsp92II CATG 5 cut(s) 261, 307, 680, 778, 896
HspAI GCGC 1 cut(s) 267
Kzo9I GATC 1 cut(s) 660
LmnI GCTCC 2 cut(s) 799, 877
Lsp1109I GCAGC 3 cut(s) 578, 669, 790
LweI GCATC 2 cut(s) 388, 417
MaeIII GTNAC 5 cut(s) 95, 150, 157, 165, 785
MalI GATC 1 cut(s) 662
MboI GATC 1 cut(s) 660
MboII GAAGA 3 cut(s) 67, 214, 524
MflI RGATCY 1 cut(s) 660
MhlI GDGCHC 1 cut(s) 432
MlsI TGGCCA 2 cut(s) 5, 302
MluCI AATT 2 cut(s) 217, 476
MluNI TGGCCA 2 cut(s) 5, 302
MmeI TCCRAC 1 cut(s) 871
Mox20I TGGCCA 2 cut(s) 5, 302
MscI TGGCCA 2 cut(s) 5, 302
MslI CAYNNNNRTG 2 cut(s) 256, 891
Msp20I TGGCCA 2 cut(s) 5, 302
MspR9I CCNGG 4 cut(s) 299, 309, 539, 716
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 4 cut(s) 299, 309, 539, 716
NcoI CCATGG 1 cut(s) 676
NdeI CATATG 1 cut(s) 364
NdeII GATC 1 cut(s) 660
NlaIII CATG 5 cut(s) 261, 307, 680, 778, 896
NlaIV GGNNCC 4 cut(s) 318, 431, 625, 838
NmuCI GTSAC 2 cut(s) 95, 165
PasI CCCWGGG 1 cut(s) 715
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 4 cut(s) 116, 649, 723, 740
PkrI GCNGC 5 cut(s) 333, 463, 568, 684, 780
PpuMI RGGWCCY 2 cut(s) 316, 623
Psp5II RGGWCCY 2 cut(s) 316, 623
Psp6I CCWGG 4 cut(s) 297, 307, 537, 714
PspEI GGTNACC 1 cut(s) 95
PspFI CCCAGC 2 cut(s) 433, 454
PspGI CCWGG 4 cut(s) 297, 307, 537, 714
PspN4I GGNNCC 4 cut(s) 318, 431, 625, 838
PspPI GGNCC 3 cut(s) 232, 316, 623
PspPPI RGGWCCY 2 cut(s) 316, 623
PsuI RGATCY 1 cut(s) 660
RsaI GTAC 2 cut(s) 39, 520
RsaNI GTAC 2 cut(s) 38, 519
RseI CAYNNNNRTG 2 cut(s) 256, 891
SatI GCNGC 5 cut(s) 332, 462, 567, 683, 779
Sau3AI GATC 1 cut(s) 660
Sau96I GGNCC 3 cut(s) 232, 316, 623
ScaI AGTACT 1 cut(s) 520
ScrFI CCNGG 4 cut(s) 299, 309, 539, 716
SduI GDGCHC 1 cut(s) 432
SetI ASST 8 cut(s) 60, 108, 177, 318, 444, 540, 571, 842
SfaNI GCATC 2 cut(s) 388, 417
SinI GGWCC 2 cut(s) 316, 623
SmiMI CAYNNNNRTG 2 cut(s) 256, 891
SmlI CTYRAG 1 cut(s) 757
SmoI CTYRAG 1 cut(s) 757
Sse9I AATT 2 cut(s) 217, 476
SsiI CCGC 3 cut(s) 332, 461, 673
StyD4I CCNGG 4 cut(s) 297, 307, 537, 714
StyI CCWWGG 2 cut(s) 630, 676
TaaI ACNGT 1 cut(s) 488
TasI AATT 2 cut(s) 217, 476
TatI WGTACW 1 cut(s) 518
TauI GCSGC 2 cut(s) 334, 464
TfiI GAWTC 4 cut(s) 116, 649, 723, 740
TscAI CASTG 3 cut(s) 580, 788, 878
TseFI GTSAC 2 cut(s) 95, 165
TseI GCWGC 3 cut(s) 566, 682, 778
Tsp45I GTSAC 2 cut(s) 95, 165
TspDTI ATGAA 6 cut(s) 282, 342, 512, 630, 695, 715
TspRI CASTG 3 cut(s) 580, 788, 878
VpaK11BI GGWCC 2 cut(s) 316, 623
XagI CCTNNNNNAGG 1 cut(s) 446
ZrmI AGTACT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.