Rh5AG300800

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018614
Physical Location & Seq
Forward (+)
42174161 .. 42177901
3741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG300800.1

Sequence Viewer

Length: 684 bp
ATGGACATTTTTGCTATTACAAATGAGTATAGAGATCTGCCGTCTTTGAAGTTTCTTAGCATAGGGCGTGGGTATCCATATACTGATGATATTCATCTCTTCCAGAGGACATCTATCAGGGGTCATCGCGCTGTGAAAACCCCCATTGTGAGGCTGAAGGGGTTGGGGCCCAACAGGGATTTTTCATATTTGAATCTGACAGGGATCTATGTGAACCACCATAGGCTGCATATTAATACACAAGTCTTTCATCCCTCCCGGGGAGGATTCATTGTGGACACAGCATCGCCGTACACTATACTCCCAGAGGAGGCCTACAGGGAATTGAGACGAGCAATCCACAATTTTTTTTCTGAACAGTATCCTGGTCTGGAGCCATTGAAGGACCCAAAATCAGGGTTTGATATGTGCTTGCCCACATCGCCGCTGGTGCTACGAAGGCCTTCAATCCAGCTCATGTTTGAAGGTGGAGCCAAGCTCGTGCTAAACCCAGCGACAACTTTCCATGAGTTTGAGGACCTCAACGAGTTCTGCCTTCTCATACTACCACGGCCTTCTTTCCATGATGGTGGTTCTTCTCCCAGTGTCTTGGGTGTTCTCCAGCAAGTCGGACACAGGTTCCACTTCAACATTCACGCTTCAATTCTGGCTTTTGCTCCCGAACTTTGTTACAGTGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

227

Amino Acids

25.78

Weight (kDa)

7.21

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_C PF14541 63 - 219 2.7e-23 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018614)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0044761
rosa_laevigata RLG00000034297
rosa_multiflora Rmu_ssc0000442.1_g000001
rosa_roxburghii Rroxscaffold_1G00036270
rosa_rugosa Rorug05G0218000
rosa_samantha Rh5AG300800 Rh5BG307600 Rh5DG317700
rosa_wichuraiana Rw5G027830 Rw5G027870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 129
AciI CCGC 1 cut(s) 425
AclWI GGATC 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 1 cut(s) 293
AfiI CCNNNNNNNGG 4 cut(s) 150, 260, 310, 395
AgsI TTSAA 7 cut(s) 49, 193, 382, 447, 464, 628, 642
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 2 cut(s) 454, 478
AluI AGCT 2 cut(s) 454, 478
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 4 cut(s) 167, 312, 440, 551
ApaI GGGCCC 1 cut(s) 171
ApeKI GCWGC 1 cut(s) 226
AseI ATTAAT 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 442
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 4 cut(s) 167, 168, 385, 517
AsuC2I CCSGG 2 cut(s) 259, 260
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 2 cut(s) 385, 517
BaeGI GKGCMC 1 cut(s) 171
BanII GRGCYC 1 cut(s) 171
BauI CACGAG 1 cut(s) 479
BbvI GCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 560
BceAI ACGGC 3 cut(s) 25, 274, 566
BciT130I CCWGG 1 cut(s) 366
BciVI GTATCC 2 cut(s) 84, 372
BcnI CCSGG 2 cut(s) 259, 260
BcoDI GTCTC 1 cut(s) 322
BfmI CTRYAG 1 cut(s) 316
BfuI GTATCC 2 cut(s) 84, 372
BglII AGATCT 1 cut(s) 34
BisI GCNGC 2 cut(s) 227, 425
BlsI GCNGC 2 cut(s) 228, 426
Bme1390I CCNGG 3 cut(s) 259, 260, 366
Bme18I GGWCC 2 cut(s) 385, 517
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 4 cut(s) 167, 168, 385, 517
BmiI GGNNCC 6 cut(s) 168, 169, 375, 387, 472, 620
BmrFI CCNGG 3 cut(s) 259, 260, 366
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 2 cut(s) 293, 667
BmuI ACTGGG 1 cut(s) 576
BplI GAGNNNNNCTC 2 cut(s) 462, 494
BpmI CTGGAG 2 cut(s) 392, 584
BpuMI CCSGG 2 cut(s) 259, 260
BsaBI GATNNNNATC 1 cut(s) 93
BsaJI CCNNGG 3 cut(s) 258, 259, 548
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 260, 310, 395
Bse1I ACTGG 1 cut(s) 582
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 3 cut(s) 258, 259, 548
BseGI GGATG 1 cut(s) 250
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 4 cut(s) 150, 260, 310, 395
BseNI ACTGG 1 cut(s) 582
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 1 cut(s) 213
BseYI CCCAGC 1 cut(s) 490
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 4 cut(s) 169, 314, 442, 553
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 1 cut(s) 259
BslI CCNNNNNNNGG 4 cut(s) 150, 260, 310, 395
BsmAI GTCTC 1 cut(s) 322
BsmBI CGTCTC 1 cut(s) 322
BsnI GGCC 4 cut(s) 169, 314, 442, 553
BsoBI CYCGRG 1 cut(s) 258
Bsp120I GGGCCC 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 2 cut(s) 34, 204
BspACI CCGC 1 cut(s) 425
BspANI GGCC 4 cut(s) 169, 314, 442, 553
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 6 cut(s) 168, 169, 375, 387, 472, 620
BspPI GGATC 1 cut(s) 212
BsrI ACTGG 1 cut(s) 582
BssECI CCNNGG 3 cut(s) 258, 259, 548
BssMI GATC 2 cut(s) 34, 204
BssSI CACGAG 1 cut(s) 479
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 2 cut(s) 360, 674
Bst6I CTCTTC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 548
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 2 cut(s) 37, 207
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 2 cut(s) 34, 204
BstMWI GCNNNNNNNGC 3 cut(s) 421, 430, 439
BstNI CCWGG 1 cut(s) 366
BstSCI CCNGG 3 cut(s) 257, 258, 364
BstSFI CTRYAG 1 cut(s) 316
BstSLI GKGCMC 1 cut(s) 171
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 213
BstX2I RGATCY 2 cut(s) 34, 204
BstXI CCANNNNNNTGG 2 cut(s) 569, 589
BstYI RGATCY 2 cut(s) 34, 204
BsuI GTATCC 2 cut(s) 84, 372
BsuRI GGCC 4 cut(s) 169, 314, 442, 553
BtgI CCRYGG 1 cut(s) 548
BtgZI GCGATG 3 cut(s) 110, 270, 405
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 2 cut(s) 589, 679
Cac8I GCNNGC 1 cut(s) 413
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 4 cut(s) 167, 168, 385, 517
Cfr9I CCCGGG 1 cut(s) 258
Csp6I GTAC 1 cut(s) 292
CviAII CATG 4 cut(s) 457, 506, 563, 681
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 1 cut(s) 56
DpnI GATC 2 cut(s) 36, 206
DpnII GATC 2 cut(s) 34, 204
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco147I AGGCCT 2 cut(s) 314, 442
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 2 cut(s) 385, 517
Eco57I CTGAAG 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 258
EcoO109I RGGNCCY 3 cut(s) 167, 385, 517
EcoRII CCWGG 1 cut(s) 364
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 4 cut(s) 460, 509, 566, 684
FatI CATG 4 cut(s) 456, 505, 562, 680
Fnu4HI GCNGC 2 cut(s) 227, 425
FokI GGATG 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 2 cut(s) 227, 425
GlaI GCGC 1 cut(s) 130
GluI GCNGC 2 cut(s) 227, 425
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 2 cut(s) 392, 584
HaeIII GGCC 4 cut(s) 169, 314, 442, 553
HapII CCGG 1 cut(s) 259
HhaI GCGC 1 cut(s) 131
Hin1II CATG 4 cut(s) 460, 509, 566, 684
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 2 cut(s) 193, 267
HpaII CCGG 1 cut(s) 259
Hpy166II GTNNAC 3 cut(s) 214, 277, 294
Hpy188I TCNGA 3 cut(s) 198, 355, 611
Hpy188III TCNNGA 3 cut(s) 103, 371, 659
Hpy8I GTNNAC 3 cut(s) 214, 277, 294
HpyAV CCTTC 7 cut(s) 151, 376, 432, 453, 458, 545, 564
HpyCH4III ACNGT 2 cut(s) 360, 674
HpyCH4V TGCA 2 cut(s) 229, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 421, 430, 439
HpyF3I CTNAG 1 cut(s) 56
Hsp92II CATG 4 cut(s) 460, 509, 566, 684
HspAI GCGC 1 cut(s) 129
Kzo9I GATC 2 cut(s) 34, 204
LmnI GCTCC 3 cut(s) 373, 470, 661
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 2 cut(s) 293, 667
MaeIII GTNAC 1 cut(s) 668
MalI GATC 2 cut(s) 36, 206
MboI GATC 2 cut(s) 34, 204
MboII GAAGA 2 cut(s) 91, 567
MflI RGATCY 2 cut(s) 34, 204
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 3 cut(s) 323, 343, 642
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 8 cut(s) 99, 144, 257, 265, 301, 304, 508, 530
Mph1103I ATGCAT 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 442
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 427
MspI CCGG 1 cut(s) 259
MspR9I CCNGG 3 cut(s) 259, 260, 366
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 421, 430, 439
NciI CCSGG 2 cut(s) 259, 260
NdeII GATC 2 cut(s) 34, 204
NlaIII CATG 4 cut(s) 460, 509, 566, 684
NlaIV GGNNCC 6 cut(s) 168, 169, 375, 387, 472, 620
NsiI ATGCAT 1 cut(s) 682
PceI AGGCCT 2 cut(s) 314, 442
PdmI GAANNNNTTC 1 cut(s) 442
PfeI GAWTC 2 cut(s) 193, 267
PkrI GCNGC 2 cut(s) 228, 426
PpuMI RGGWCCY 2 cut(s) 385, 517
PshBI ATTAAT 1 cut(s) 234
Psp5II RGGWCCY 2 cut(s) 385, 517
Psp6I CCWGG 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 6 cut(s) 168, 169, 375, 387, 472, 620
PspOMI GGGCCC 1 cut(s) 167
PspPI GGNCC 4 cut(s) 167, 168, 385, 517
PspPPI RGGWCCY 2 cut(s) 385, 517
PsuI RGATCY 2 cut(s) 34, 204
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 567
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 2 cut(s) 227, 425
Sau3AI GATC 2 cut(s) 34, 204
Sau96I GGNCC 4 cut(s) 167, 168, 385, 517
ScrFI CCNGG 3 cut(s) 259, 260, 366
SduI GDGCHC 1 cut(s) 171
SetI ASST 5 cut(s) 456, 469, 480, 522, 620
SfaNI GCATC 2 cut(s) 293, 667
SfcI CTRYAG 1 cut(s) 316
SinI GGWCC 2 cut(s) 385, 517
SmaI CCCGGG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 567
Sse9I AATT 3 cut(s) 323, 343, 642
SseBI AGGCCT 2 cut(s) 314, 442
SsiI CCGC 1 cut(s) 425
StuI AGGCCT 2 cut(s) 314, 442
StyD4I CCNGG 3 cut(s) 257, 258, 364
TaaI ACNGT 2 cut(s) 360, 674
TasI AATT 3 cut(s) 323, 343, 642
TauI GCSGC 1 cut(s) 427
TfiI GAWTC 2 cut(s) 193, 267
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 2 cut(s) 589, 679
TseI GCWGC 1 cut(s) 226
TspDTI ATGAA 4 cut(s) 83, 174, 239, 259
TspMI CCCGGG 1 cut(s) 258
TspRI CASTG 2 cut(s) 589, 679
VpaK11BI GGWCC 2 cut(s) 385, 517
VspI ATTAAT 1 cut(s) 234
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmaI CCCGGG 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 442
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.