Rroxscaffold_1G00036270

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018614
Physical Location & Seq
Reverse (-)
52901723 .. 52907340
5618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00036270.1

Sequence Viewer

Length: 909 bp
ATGGCCAAGCCCCAGTTCCTCCTCCTCCTTCTCCTCGTACTCCTCCTCAACTGCAACCTAACATCCCCGTCAGTCTTCAAGATACACTCATCTGGTCACCAAAAGCTCTCTACTGATTCCACCACCATCCCTCAGCCCCCACCAGCAAAGTTACTCATTACTCAGTCACGAGACCTATATTTTGGAGTTGAAATCAACATAGGCATACCCACCCAGAATTTCTTCAATCTGGCCCTTGATATAAAAAATCCATTCACCTGGGTTGGCACCTCCAAGCCAAACATCTCGCATTCGTTCCTGGCCATGCCAGGAAAGGACCCGCTGTGCCTGCCGCCTCTGTTCGCCTCTGCCACATCCTGCGCATATGACTTCGGTTATGCTCGTGGGATTTTTGGGTTTGATTATTTCTTGGGCATAGAATCTCAGACCAAGGCTCCCTTTGGCTGGGGGATTTTTACAGACGAACGGGAGGAGGGTGTGCTACGTAGTACTAGAGATGCAGATGGCATCTTAGGGTTGGGAGTAGGGCACCCAGCCAACTTGTTAGAGGGGCTGGGGGCGGCAACTCTTGGCCAATTCTCTAACTGTTTGCCAATGAACTTTATGACTGAAGAAAGCTTCTTGCAGATCACTAATAGTACTGACCGGCAACACTACCCTGCGACTTTAAACTTTGGAGAAAATGCAGCTATCAGGGGTCATCGCGCTGTGAAAACCCCCATTGTGAGGCTGAAGGGGTTGGCGCCCACCAGGGATTTTTCATATTTGAATCTGACAGGAATCTATGTGAACCACCATAGGCTGCATATTAATACACAAGTCTTTCATCCCTCCCAGGGAGGATTCATTGTGGACACAGAGGAGGCCTACAAGGAATTGAGACGAGCAATCCACAATTTTTTTTTCTGA

Protein Analysis

302

Amino Acids

33.49

Weight (kDa)

7.18

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018614)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0044761
rosa_laevigata RLG00000034297
rosa_multiflora Rmu_ssc0000442.1_g000001
rosa_roxburghii Rroxscaffold_1G00036270
rosa_rugosa Rorug05G0218000
rosa_samantha Rh5AG300800 Rh5BG307600 Rh5DG317700
rosa_wichuraiana Rw5G027830 Rw5G027870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 361
AccB1I GGYRCC 3 cut(s) 266, 528, 742
AccII CGCG 1 cut(s) 705
AciI CCGC 3 cut(s) 320, 332, 560
AcoI YGGCCR 3 cut(s) 3, 300, 571
AcsI RAATTY 1 cut(s) 217
AcuI CTGAAG 2 cut(s) 630, 752
AcyI GRCGYC 1 cut(s) 743
AfaI GTAC 3 cut(s) 39, 490, 640
AfiI CCNNNNNNNGG 3 cut(s) 444, 726, 836
AgsI TTSAA 4 cut(s) 79, 191, 226, 769
AjnI CCWGG 5 cut(s) 257, 297, 307, 749, 834
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 3 cut(s) 106, 618, 689
AluI AGCT 3 cut(s) 106, 618, 689
Alw26I GTCTC 2 cut(s) 165, 874
AoxI GGCC 5 cut(s) 3, 231, 300, 571, 864
ApeKI GCWGC 2 cut(s) 686, 802
ApoI RAATTY 1 cut(s) 217
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AseI ATTAAT 1 cut(s) 810
Asp700I GAANNNNTTC 1 cut(s) 221
AspLEI GCGC 3 cut(s) 362, 707, 745
AspS9I GGNCC 2 cut(s) 232, 316
AsuHPI GGTGA 2 cut(s) 89, 247
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 531
BalI TGGCCA 3 cut(s) 5, 302, 573
BanI GGYRCC 3 cut(s) 266, 528, 742
BauI CACGAG 2 cut(s) 168, 381
BbsI GAAGAC 1 cut(s) 67
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 2 cut(s) 698, 789
BccI CCATC 2 cut(s) 134, 497
BciT130I CCWGG 5 cut(s) 259, 299, 309, 751, 836
BcoDI GTCTC 2 cut(s) 165, 874
BfaI CTAG 1 cut(s) 492
BfoI RGCGCY 1 cut(s) 746
BisI GCNGC 4 cut(s) 332, 561, 687, 803
BlsI GCNGC 4 cut(s) 333, 562, 688, 804
BmcAI AGTACT 2 cut(s) 490, 640
Bme1390I CCNGG 5 cut(s) 259, 299, 309, 751, 836
Bme18I GGWCC 1 cut(s) 316
BmgT120I GGNCC 2 cut(s) 232, 316
BmiI GGNNCC 5 cut(s) 268, 318, 435, 530, 744
BmrFI CCNGG 5 cut(s) 259, 299, 309, 751, 836
BmrI ACTGGG 1 cut(s) 7
BmsI GCATC 2 cut(s) 487, 516
BmuI ACTGGG 1 cut(s) 7
BpiI GAAGAC 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 485
BsaHI GRCGYC 1 cut(s) 743
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 5 cut(s) 258, 429, 750, 834, 835
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 444, 726, 836
Bse118I RCCGGY 1 cut(s) 645
Bse1I ACTGG 1 cut(s) 13
BseBI CCWGG 5 cut(s) 259, 299, 309, 751, 836
BseDI CCNNGG 5 cut(s) 258, 429, 750, 834, 835
BseGI GGATG 4 cut(s) 62, 126, 353, 826
BseLI CCNNNNNNNGG 3 cut(s) 444, 726, 836
BseMII CTCAG 3 cut(s) 146, 176, 437
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 7 cut(s) 11, 14, 23, 32, 35, 485, 875
BseSI GKGCMC 1 cut(s) 531
BseXI GCAGC 2 cut(s) 698, 789
BseYI CCCAGC 3 cut(s) 444, 532, 553
Bsh1236I CGCG 1 cut(s) 705
BshFI GGCC 5 cut(s) 5, 233, 302, 573, 866
BshNI GGYRCC 3 cut(s) 266, 528, 742
BsiSI CCGG 1 cut(s) 646
BslI CCNNNNNNNGG 3 cut(s) 444, 726, 836
BsmAI GTCTC 2 cut(s) 165, 874
BsmBI CGTCTC 1 cut(s) 874
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 5 cut(s) 5, 233, 302, 573, 866
Bso31I GGTCTC 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 1 cut(s) 627
BspACI CCGC 3 cut(s) 320, 332, 560
BspANI GGCC 5 cut(s) 5, 233, 302, 573, 866
BspCNI CTCAG 3 cut(s) 145, 175, 436
BspFNI CGCG 1 cut(s) 705
BspLI GGNNCC 5 cut(s) 268, 318, 435, 530, 744
BspT107I GGYRCC 3 cut(s) 266, 528, 742
BspTNI GGTCTC 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 645
BsrI ACTGG 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 645
BssECI CCNNGG 5 cut(s) 258, 429, 750, 834, 835
BssMI GATC 1 cut(s) 627
BssNI GRCGYC 1 cut(s) 743
BssSI CACGAG 2 cut(s) 168, 381
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 2 cut(s) 168, 381
Bst2UI CCWGG 5 cut(s) 259, 299, 309, 751, 836
Bst4CI ACNGT 1 cut(s) 587
BstACI GRCGYC 1 cut(s) 743
BstBAI YACGTR 1 cut(s) 485
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 4 cut(s) 132, 162, 423, 511
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 4 cut(s) 62, 126, 353, 826
BstFNI CGCG 1 cut(s) 705
BstH2I RGCGCY 1 cut(s) 746
BstHHI GCGC 3 cut(s) 362, 707, 745
BstKTI GATC 1 cut(s) 630
BstMAI GTCTC 2 cut(s) 165, 874
BstMBI GATC 1 cut(s) 627
BstMWI GCNNNNNNNGC 1 cut(s) 328
BstNI CCWGG 5 cut(s) 259, 299, 309, 751, 836
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 5 cut(s) 257, 297, 307, 749, 834
BstSLI GKGCMC 1 cut(s) 531
BstSNI TACGTA 1 cut(s) 485
BstUI CGCG 1 cut(s) 705
BstV1I GCAGC 2 cut(s) 698, 789
BstV2I GAAGAC 1 cut(s) 67
BstXI CCANNNNNNTGG 1 cut(s) 258
BsuRI GGCC 5 cut(s) 5, 233, 302, 573, 866
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 4 cut(s) 62, 126, 353, 826
Cac8I GCNNGC 1 cut(s) 329
CfoI GCGC 3 cut(s) 362, 707, 745
Cfr10I RCCGGY 1 cut(s) 645
Cfr13I GGNCC 2 cut(s) 232, 316
Csp6I GTAC 3 cut(s) 38, 489, 639
CviAII CATG 1 cut(s) 304
CviQI GTAC 3 cut(s) 38, 489, 639
DdeI CTNAG 4 cut(s) 132, 162, 423, 511
DinI GGCGCC 1 cut(s) 744
DpnI GATC 1 cut(s) 629
DpnII GATC 1 cut(s) 627
DraI TTTAAA 1 cut(s) 669
EaeI YGGCCR 3 cut(s) 3, 300, 571
Eco105I TACGTA 1 cut(s) 485
Eco130I CCWWGG 1 cut(s) 429
Eco147I AGGCCT 1 cut(s) 866
Eco31I GGTCTC 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 630, 752
Eco91I GGTNACC 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 316
EcoO65I GGTNACC 1 cut(s) 95
EcoRII CCWGG 5 cut(s) 257, 297, 307, 749, 834
EcoT14I CCWWGG 1 cut(s) 429
EgeI GGCGCC 1 cut(s) 744
EheI GGCGCC 1 cut(s) 744
ErhI CCWWGG 1 cut(s) 429
Esp3I CGTCTC 1 cut(s) 874
FaeI CATG 1 cut(s) 307
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FatI CATG 1 cut(s) 303
FauI CCCGC 1 cut(s) 327
FauNDI CATATG 1 cut(s) 364
Fnu4HI GCNGC 4 cut(s) 332, 561, 687, 803
FokI GGATG 4 cut(s) 49, 113, 340, 813
Fsp4HI GCNGC 4 cut(s) 332, 561, 687, 803
FspBI CTAG 1 cut(s) 492
FspI TGCGCA 1 cut(s) 361
GlaI GCGC 3 cut(s) 361, 706, 744
GluI GCNGC 4 cut(s) 332, 561, 687, 803
GsaI CCCAGC 3 cut(s) 448, 536, 557
HaeII RGCGCY 1 cut(s) 746
HaeIII GGCC 5 cut(s) 5, 233, 302, 573, 866
HapII CCGG 1 cut(s) 646
HhaI GCGC 3 cut(s) 362, 707, 745
Hin1I GRCGYC 1 cut(s) 743
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 3 cut(s) 360, 705, 743
HinP1I GCGC 3 cut(s) 360, 705, 743
HindIII AAGCTT 1 cut(s) 616
HinfI GANTC 5 cut(s) 116, 419, 769, 780, 843
HpaII CCGG 1 cut(s) 646
HphI GGTGA 2 cut(s) 89, 247
Hpy166II GTNNAC 2 cut(s) 790, 853
Hpy188I TCNGA 3 cut(s) 426, 774, 908
Hpy188III TCNNGA 2 cut(s) 79, 168
Hpy8I GTNNAC 2 cut(s) 790, 853
HpyAV CCTTC 2 cut(s) 38, 727
HpyCH4III ACNGT 1 cut(s) 587
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 5 cut(s) 54, 500, 625, 686, 805
HpyF10VI GCNNNNNNNGC 1 cut(s) 328
HpyF3I CTNAG 4 cut(s) 132, 162, 423, 511
HpySE526I ACGT 1 cut(s) 484
Hsp92I GRCGYC 1 cut(s) 743
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 3 cut(s) 360, 705, 743
KasI GGCGCC 1 cut(s) 742
Kzo9I GATC 1 cut(s) 627
LmnI GCTCC 1 cut(s) 439
Lsp1109I GCAGC 2 cut(s) 698, 789
LweI GCATC 2 cut(s) 487, 516
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 3 cut(s) 95, 150, 165
MalI GATC 1 cut(s) 629
MboI GATC 1 cut(s) 627
MboII GAAGA 3 cut(s) 67, 214, 623
MhlI GDGCHC 1 cut(s) 531
MlsI TGGCCA 3 cut(s) 5, 302, 573
MluCI AATT 4 cut(s) 217, 575, 875, 895
MluNI TGGCCA 3 cut(s) 5, 302, 573
Mly113I GGCGCC 1 cut(s) 743
Mox20I TGGCCA 3 cut(s) 5, 302, 573
MroXI GAANNNNTTC 1 cut(s) 221
MscI TGGCCA 3 cut(s) 5, 302, 573
MseI TTAA 2 cut(s) 668, 810
Msp20I TGGCCA 3 cut(s) 5, 302, 573
MspA1I CMGCKG 1 cut(s) 322
MspI CCGG 1 cut(s) 646
MspR9I CCNGG 5 cut(s) 259, 299, 309, 751, 836
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 5 cut(s) 259, 299, 309, 751, 836
MvnI CGCG 1 cut(s) 705
MwoI GCNNNNNNNGC 1 cut(s) 328
NarI GGCGCC 1 cut(s) 743
NdeI CATATG 1 cut(s) 364
NdeII GATC 1 cut(s) 627
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 5 cut(s) 268, 318, 435, 530, 744
NmuCI GTSAC 2 cut(s) 95, 165
NsbI TGCGCA 1 cut(s) 361
PasI CCCWGGG 1 cut(s) 835
PceI AGGCCT 1 cut(s) 866
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 5 cut(s) 116, 419, 769, 780, 843
PkrI GCNGC 4 cut(s) 333, 562, 688, 804
PluTI GGCGCC 1 cut(s) 746
Ppu21I YACGTR 1 cut(s) 485
PpuMI RGGWCCY 1 cut(s) 316
PshBI ATTAAT 1 cut(s) 810
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 5 cut(s) 257, 297, 307, 749, 834
PspEI GGTNACC 1 cut(s) 95
PspFI CCCAGC 3 cut(s) 444, 532, 553
PspGI CCWGG 5 cut(s) 257, 297, 307, 749, 834
PspN4I GGNNCC 5 cut(s) 268, 318, 435, 530, 744
PspPI GGNCC 2 cut(s) 232, 316
PspPPI RGGWCCY 1 cut(s) 316
RsaI GTAC 3 cut(s) 39, 490, 640
RsaNI GTAC 3 cut(s) 38, 489, 639
SaqAI TTAA 2 cut(s) 668, 810
SatI GCNGC 4 cut(s) 332, 561, 687, 803
Sau3AI GATC 1 cut(s) 627
Sau96I GGNCC 2 cut(s) 232, 316
ScaI AGTACT 2 cut(s) 490, 640
ScrFI CCNGG 5 cut(s) 259, 299, 309, 751, 836
SduI GDGCHC 1 cut(s) 531
SetI ASST 8 cut(s) 60, 108, 177, 260, 272, 487, 620, 691
SfaNI GCATC 2 cut(s) 487, 516
SfoI GGCGCC 1 cut(s) 744
SinI GGWCC 1 cut(s) 316
SnaBI TACGTA 1 cut(s) 485
Sse9I AATT 4 cut(s) 217, 575, 875, 895
SseBI AGGCCT 1 cut(s) 866
SsiI CCGC 3 cut(s) 320, 332, 560
SspDI GGCGCC 1 cut(s) 742
SspMI CTAG 1 cut(s) 492
StuI AGGCCT 1 cut(s) 866
StyD4I CCNGG 5 cut(s) 257, 297, 307, 749, 834
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 1 cut(s) 587
TaiI ACGT 1 cut(s) 487
TasI AATT 4 cut(s) 217, 575, 875, 895
TatI WGTACW 2 cut(s) 488, 638
TauI GCSGC 2 cut(s) 334, 563
TfiI GAWTC 5 cut(s) 116, 419, 769, 780, 843
Tru1I TTAA 2 cut(s) 668, 810
Tru9I TTAA 2 cut(s) 668, 810
TseFI GTSAC 2 cut(s) 95, 165
TseI GCWGC 2 cut(s) 686, 802
Tsp45I GTSAC 2 cut(s) 95, 165
TspDTI ATGAA 4 cut(s) 611, 750, 815, 835
VpaK11BI GGWCC 1 cut(s) 316
VspI ATTAAT 1 cut(s) 810
XapI RAATTY 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 1 cut(s) 492
ZrmI AGTACT 2 cut(s) 490, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.