Rw5G027870

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0018614
Physical Location & Seq
Forward (+)
41515296 .. 41516016
721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027870.1

Sequence Viewer

Length: 597 bp
ATGGGTTGGCACCTCCTCCAAGCCAAACATCTCGCATTCATTCCTGGTCATGCCAGGAAAGGACCTGCTGTGCCTGCCGCTTCTGCTGCATCTTGCGCATATGACTTTGGTTATGCTCATGGGATGTGTGGGTTTGATTATTTCTTGGGCATAGAATCTCAGATCAAGGCTCCCCTTGGCTGGGGGATTTTTACAGACGAACGGGAGGAGGGCGTGCGAGGCAGTAGTAGAGATGCAGATGGCATCTTAGGGTTGGGAGTAGGGCACCCAGCCAACCTGTTAGAGGGGCTGGGGGCGGCAACTCTTTATCAATTCTCTTACTGTTTGCCACTGAACTATGTGATTGAAGAAAGTACTGACCAGCAACGCCACCCGGCTACTCTAAACTTCGGAGAAAATGCAGCTATCAGGGGTCGTCGGGCTGTGAGAACACCCATTCTAAGGCTGAAAGGGTCAGGACCCACCAAGGATTTTTCATATCTGAATCTGACAGGGATCTATGTGAACCGCCAGAGGCTGCATATAAACACACAAGTCTTTCATCCCTCCCAGGGAGGATTCATTGTGGACACAGCATCGCCGTACTGGAAGAAGTGA

Protein Analysis

198

Amino Acids

21.65

Weight (kDa)

8.78

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018614)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0044761
rosa_laevigata RLG00000034297
rosa_multiflora Rmu_ssc0000442.1_g000001
rosa_roxburghii Rroxscaffold_1G00036270
rosa_rugosa Rorug05G0218000
rosa_samantha Rh5AG300800 Rh5BG307600 Rh5DG317700
rosa_wichuraiana Rw5G027830 Rw5G027870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 97
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 2 cut(s) 9, 264
AciI CCGC 3 cut(s) 78, 296, 508
AclWI GGATC 1 cut(s) 503
AfaI GTAC 2 cut(s) 355, 584
AfiI CCNNNNNNNGG 5 cut(s) 180, 181, 283, 441, 551
AgsI TTSAA 1 cut(s) 347
AjnI CCWGG 3 cut(s) 43, 53, 549
AluBI AGCT 1 cut(s) 404
AluI AGCT 1 cut(s) 404
AlwI GGATC 1 cut(s) 503
AlwNI CAGNNNCTG 1 cut(s) 517
ApeKI GCWGC 3 cut(s) 86, 401, 517
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 2 cut(s) 62, 458
AsuC2I CCSGG 1 cut(s) 374
AvaII GGWCC 2 cut(s) 62, 458
BaeGI GKGCMC 1 cut(s) 267
BanI GGYRCC 2 cut(s) 9, 264
BbvI GCAGC 3 cut(s) 73, 413, 504
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 565
BciT130I CCWGG 3 cut(s) 45, 55, 551
BcnI CCSGG 1 cut(s) 374
BfuAI ACCTGC 1 cut(s) 73
BisI GCNGC 5 cut(s) 78, 87, 297, 402, 518
BlsI GCNGC 5 cut(s) 79, 88, 298, 403, 519
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 4 cut(s) 45, 55, 374, 551
Bme18I GGWCC 2 cut(s) 62, 458
BmgT120I GGNCC 2 cut(s) 62, 458
BmiI GGNNCC 4 cut(s) 11, 171, 266, 460
BmrFI CCNGG 4 cut(s) 45, 55, 374, 551
BmsI GCATC 4 cut(s) 98, 223, 252, 584
BpuMI CCSGG 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 175, 465, 549, 550
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 181, 283, 441, 551
Bse1I ACTGG 1 cut(s) 590
BseBI CCWGG 3 cut(s) 45, 55, 551
BseDI CCNNGG 4 cut(s) 175, 465, 549, 550
BseGI GGATG 2 cut(s) 129, 541
BseLI CCNNNNNNNGG 5 cut(s) 180, 181, 283, 441, 551
BseMII CTCAG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 2 cut(s) 5, 221
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 3 cut(s) 73, 413, 504
BseYI CCCAGC 3 cut(s) 180, 268, 289
BshNI GGYRCC 2 cut(s) 9, 264
BsiSI CCGG 1 cut(s) 374
BslI CCNNNNNNNGG 5 cut(s) 180, 181, 283, 441, 551
BsmI GAATGC 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 2 cut(s) 162, 495
BspACI CCGC 3 cut(s) 78, 296, 508
BspCNI CTCAG 1 cut(s) 172
BspLI GGNNCC 4 cut(s) 11, 171, 266, 460
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 1 cut(s) 503
BspT107I GGYRCC 2 cut(s) 9, 264
BsrI ACTGG 1 cut(s) 590
BssECI CCNNGG 4 cut(s) 175, 465, 549, 550
BssMI GATC 2 cut(s) 162, 495
BssT1I CCWWGG 2 cut(s) 175, 465
Bst2UI CCWGG 3 cut(s) 45, 55, 551
Bst4CI ACNGT 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 75, 215
BstDEI CTNAG 3 cut(s) 159, 247, 440
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 129, 541
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 2 cut(s) 165, 498
BstMBI GATC 2 cut(s) 162, 495
BstMWI GCNNNNNNNGC 5 cut(s) 74, 83, 86, 95, 219
BstNI CCWGG 3 cut(s) 45, 55, 551
BstSCI CCNGG 4 cut(s) 43, 53, 372, 549
BstSLI GKGCMC 1 cut(s) 267
BstV1I GCAGC 3 cut(s) 73, 413, 504
BstX2I RGATCY 1 cut(s) 495
BstYI RGATCY 1 cut(s) 495
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 2 cut(s) 129, 541
BtsIMutI CAGTG 1 cut(s) 329
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 75, 215
CaiI CAGNNNCTG 1 cut(s) 517
CfoI GCGC 1 cut(s) 98
Cfr13I GGNCC 2 cut(s) 62, 458
Csp6I GTAC 2 cut(s) 354, 583
CviAII CATG 2 cut(s) 50, 119
CviQI GTAC 2 cut(s) 354, 583
DdeI CTNAG 3 cut(s) 159, 247, 440
DpnI GATC 2 cut(s) 164, 497
DpnII GATC 2 cut(s) 162, 495
Eco130I CCWWGG 2 cut(s) 175, 465
Eco47I GGWCC 2 cut(s) 62, 458
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoO109I RGGNCCY 2 cut(s) 62, 458
EcoRII CCWGG 3 cut(s) 43, 53, 549
EcoT14I CCWWGG 2 cut(s) 175, 465
ErhI CCWWGG 2 cut(s) 175, 465
FaeI CATG 2 cut(s) 53, 122
FatI CATG 2 cut(s) 49, 118
FauNDI CATATG 1 cut(s) 100
Fnu4HI GCNGC 5 cut(s) 78, 87, 297, 402, 518
FokI GGATG 2 cut(s) 136, 528
Fsp4HI GCNGC 5 cut(s) 78, 87, 297, 402, 518
FspI TGCGCA 1 cut(s) 97
GlaI GCGC 1 cut(s) 97
GluI GCNGC 5 cut(s) 78, 87, 297, 402, 518
GsaI CCCAGC 3 cut(s) 184, 272, 293
HapII CCGG 1 cut(s) 374
HhaI GCGC 1 cut(s) 98
Hin1II CATG 2 cut(s) 53, 122
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HinfI GANTC 3 cut(s) 155, 484, 558
HpaII CCGG 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 505, 568
Hpy188I TCNGA 4 cut(s) 162, 392, 483, 489
Hpy188III TCNNGA 1 cut(s) 456
Hpy8I GTNNAC 2 cut(s) 505, 568
Hpy99I CGWCG 1 cut(s) 420
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4V TGCA 4 cut(s) 89, 236, 401, 520
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 83, 86, 95, 219
HpyF3I CTNAG 3 cut(s) 159, 247, 440
Hsp92II CATG 2 cut(s) 53, 122
HspAI GCGC 1 cut(s) 96
Kzo9I GATC 2 cut(s) 162, 495
LmnI GCTCC 1 cut(s) 175
Lsp1109I GCAGC 3 cut(s) 73, 413, 504
LweI GCATC 4 cut(s) 98, 223, 252, 584
MalI GATC 2 cut(s) 164, 497
MboI GATC 2 cut(s) 162, 495
MboII GAAGA 1 cut(s) 359
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 311
MnlI CCTC 9 cut(s) 23, 26, 199, 202, 212, 277, 507, 548, 556
MspI CCGG 1 cut(s) 374
MspR9I CCNGG 4 cut(s) 45, 55, 374, 551
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 3 cut(s) 45, 55, 551
MwoI GCNNNNNNNGC 5 cut(s) 74, 83, 86, 95, 219
NciI CCSGG 1 cut(s) 374
NdeI CATATG 1 cut(s) 100
NdeII GATC 2 cut(s) 162, 495
NlaIII CATG 2 cut(s) 53, 122
NlaIV GGNNCC 4 cut(s) 11, 171, 266, 460
NsbI TGCGCA 1 cut(s) 97
PasI CCCWGGG 1 cut(s) 550
PctI GAATGC 1 cut(s) 35
PfeI GAWTC 3 cut(s) 155, 484, 558
PkrI GCNGC 5 cut(s) 79, 88, 298, 403, 519
PpuMI RGGWCCY 2 cut(s) 62, 458
Psp5II RGGWCCY 2 cut(s) 62, 458
Psp6I CCWGG 3 cut(s) 43, 53, 549
PspFI CCCAGC 3 cut(s) 180, 268, 289
PspGI CCWGG 3 cut(s) 43, 53, 549
PspN4I GGNNCC 4 cut(s) 11, 171, 266, 460
PspPI GGNCC 2 cut(s) 62, 458
PspPPI RGGWCCY 2 cut(s) 62, 458
PstNI CAGNNNCTG 1 cut(s) 517
PsuI RGATCY 1 cut(s) 495
RsaI GTAC 2 cut(s) 355, 584
RsaNI GTAC 2 cut(s) 354, 583
SatI GCNGC 5 cut(s) 78, 87, 297, 402, 518
Sau3AI GATC 2 cut(s) 162, 495
Sau96I GGNCC 2 cut(s) 62, 458
ScaI AGTACT 1 cut(s) 355
ScrFI CCNGG 4 cut(s) 45, 55, 374, 551
SduI GDGCHC 1 cut(s) 267
SetI ASST 4 cut(s) 15, 67, 279, 406
SfaNI GCATC 4 cut(s) 98, 223, 252, 584
SinI GGWCC 2 cut(s) 62, 458
Sse9I AATT 1 cut(s) 311
SsiI CCGC 3 cut(s) 78, 296, 508
StyD4I CCNGG 4 cut(s) 43, 53, 372, 549
StyI CCWWGG 2 cut(s) 175, 465
TaaI ACNGT 1 cut(s) 323
TasI AATT 1 cut(s) 311
TatI WGTACW 1 cut(s) 353
TauI GCSGC 2 cut(s) 80, 299
TfiI GAWTC 3 cut(s) 155, 484, 558
TscAI CASTG 1 cut(s) 336
TseI GCWGC 3 cut(s) 86, 401, 517
TspDTI ATGAA 4 cut(s) 28, 465, 530, 550
TspRI CASTG 1 cut(s) 336
VpaK11BI GGWCC 2 cut(s) 62, 458
XagI CCTNNNNNAGG 1 cut(s) 281
ZrmI AGTACT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.