RLG00000036259

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017635
Physical Location & Seq
Forward (+)
78149176 .. 78149619
444 bp
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UTR
Exon/CDS
Intron
RLM00000036259

Sequence Viewer

Length: 444 bp
ATGCCGGAAAATCCCCAATCCCGCCACAGAAAAATTGTGAAGACCCTCTACAAAACCCTAGCACACAGAGACACCAGAACCACCGTGATGCTAGTGGCTCCAGATCTCGAGTGGTGGTTCCACGGACCACCGCAGTGCCAGCACATGATGAAGATGCTAACCGGGCAGTCGAGGCACTTGGAGTTCAAGTTCCAGCCCCGAAGCCTCAAGGCGGTTGGGGACCGTGTGGTGGCGGAGGGATGGGAAGGTAAGAAGGCATATTGGGTACAGGTGTGGACTGTTAACAAGGATGGGATGATCACGCAGTTACGCGAGTATTTCAACACTTGGGTGACGTTGATGGTTAGGGTTTCGGAGGCCGAGGACGAGATGGTGAAGCTTTGGCAGAGTGATTCAAAGGAGCGGTTCCGGCGCTCGTTGCCGGATATTGTGTTAGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WI12

Protein Analysis

148

Amino Acids

17.47

Weight (kDa)

9.62

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 50 - 147 4.9e-27 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39910
prunus_persica Prupe.6G046300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071881
rosa_laevigata RLG00000036259
rosa_multiflora Rmu_sc0002149.1_g000007 Rmu_sc0015603.1_g000001
rosa_roxburghii Rroxscaffold_1G00009280
rosa_rugosa Rorug05G0415300
rosa_samantha Rh5BG491300 Rh5CG515200 Rh5DG502800
rosa_wichuraiana Rw5G043810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 403
AccII CGCG 1 cut(s) 312
AciI CCGC 5 cut(s) 22, 131, 212, 233, 403
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 2 cut(s) 211, 229
AgsI TTSAA 3 cut(s) 187, 322, 396
AleI CACNNNNGTG 2 cut(s) 133, 329
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 2 cut(s) 379, 437
AluI AGCT 2 cut(s) 379, 437
Alw26I GTCTC 1 cut(s) 63
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 1 cut(s) 357
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 125, 220
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 343, 385
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 2 cut(s) 125, 220
BaeI ACNNNNGTAYC 2 cut(s) 257, 290
BarI GAAGNNNNNNTAC 2 cut(s) 32, 64
BbsI GAAGAC 1 cut(s) 47
BccI CCATC 4 cut(s) 234, 284, 334, 364
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 2 cut(s) 59, 92
BfoI RGCGCY 1 cut(s) 415
BglII AGATCT 1 cut(s) 103
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 2 cut(s) 125, 220
BmeT110I CYCGRG 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 125, 220
BmiI GGNNCC 4 cut(s) 99, 119, 221, 407
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 2 cut(s) 78, 144
BpiI GAAGAC 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 121, 360
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 229
BseDI CCNNGG 2 cut(s) 121, 360
BseGI GGATG 3 cut(s) 245, 295, 300
BseLI CCNNNNNNNGG 2 cut(s) 211, 229
Bsh1236I CGCG 1 cut(s) 312
BshFI GGCC 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 4 cut(s) 5, 162, 409, 422
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 211, 229
BsmAI GTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 107
Bsp143I GATC 2 cut(s) 103, 297
BspACI CCGC 5 cut(s) 22, 131, 212, 233, 403
BspANI GGCC 1 cut(s) 359
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 4 cut(s) 99, 119, 221, 407
BsrBI CCGCTC 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 121, 360
BssMI GATC 2 cut(s) 103, 297
Bst4CI ACNGT 3 cut(s) 85, 224, 280
BstC8I GCNNGC 1 cut(s) 140
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 3 cut(s) 245, 295, 300
BstFNI CGCG 1 cut(s) 312
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 2 cut(s) 106, 300
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 2 cut(s) 103, 297
BstMWI GCNNNNNNNGC 5 cut(s) 139, 163, 172, 409, 418
BstSCI CCNGG 1 cut(s) 161
BstUI CGCG 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 47
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 1 cut(s) 359
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 3 cut(s) 245, 295, 300
BtsI GCAGTG 1 cut(s) 140
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 140
CfoI GCGC 1 cut(s) 414
Cfr13I GGNCC 2 cut(s) 125, 220
Csp6I GTAC 1 cut(s) 266
CviAII CATG 1 cut(s) 145
CviJI RGCY 6 cut(s) 98, 196, 204, 359, 379, 437
CviKI_1 RGCY 6 cut(s) 98, 196, 204, 359, 379, 437
CviQI GTAC 1 cut(s) 266
DpnI GATC 2 cut(s) 105, 299
DpnII GATC 2 cut(s) 103, 297
EciI GGCGGA 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 125, 220
Eco88I CYCGRG 1 cut(s) 107
FaeI CATG 1 cut(s) 148
FaiI YATR 2 cut(s) 146, 259
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 144
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 1 cut(s) 297
FokI GGATG 3 cut(s) 252, 302, 307
FspBI CTAG 2 cut(s) 59, 92
GlaI GCGC 1 cut(s) 413
GsuI CTGGAG 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 1 cut(s) 359
HapII CCGG 4 cut(s) 5, 162, 409, 422
HhaI GCGC 1 cut(s) 414
Hin1II CATG 1 cut(s) 148
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 1 cut(s) 392
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 4 cut(s) 5, 162, 409, 422
HphI GGTGA 2 cut(s) 343, 385
Hpy166II GTNNAC 2 cut(s) 276, 283
Hpy188I TCNGA 2 cut(s) 355, 443
Hpy188III TCNNGA 2 cut(s) 101, 107
Hpy8I GTNNAC 2 cut(s) 276, 283
HpyAV CCTTC 2 cut(s) 239, 247
HpyCH4III ACNGT 3 cut(s) 85, 224, 280
HpyCH4IV ACGT 1 cut(s) 335
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 163, 172, 409, 418
HpySE526I ACGT 1 cut(s) 335
Hsp92II CATG 1 cut(s) 148
HspAI GCGC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 103, 297
LmnI GCTCC 2 cut(s) 103, 400
LpnPI CCDG 9 cut(s) 18, 88, 114, 152, 175, 206, 254, 422, 435
LweI GCATC 2 cut(s) 78, 144
MaeI CTAG 2 cut(s) 59, 92
MaeII ACGT 1 cut(s) 335
MaeIII GTNAC 2 cut(s) 306, 331
MalI GATC 2 cut(s) 105, 299
MbiI CCGCTC 1 cut(s) 403
MboI GATC 2 cut(s) 103, 297
MboII GAAGA 2 cut(s) 52, 163
MflI RGATCY 1 cut(s) 103
MluCI AATT 1 cut(s) 33
MnlI CCTC 6 cut(s) 56, 165, 215, 229, 349, 355
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 3 cut(s) 86, 133, 329
MspI CCGG 4 cut(s) 5, 162, 409, 422
MspR9I CCNGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 5 cut(s) 139, 163, 172, 409, 418
NciI CCSGG 1 cut(s) 163
NdeII GATC 2 cut(s) 103, 297
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 4 cut(s) 99, 119, 221, 407
NmeAIII GCCGAG 1 cut(s) 385
NmuCI GTSAC 1 cut(s) 331
OliI CACNNNNGTG 2 cut(s) 133, 329
PaeR7I CTCGAG 1 cut(s) 107
PfeI GAWTC 1 cut(s) 392
PspN4I GGNNCC 4 cut(s) 99, 119, 221, 407
PspPI GGNCC 2 cut(s) 125, 220
PsuI RGATCY 1 cut(s) 103
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
RseI CAYNNNNRTG 3 cut(s) 86, 133, 329
SaqAI TTAA 1 cut(s) 282
Sau3AI GATC 2 cut(s) 103, 297
Sau96I GGNCC 2 cut(s) 125, 220
ScrFI CCNGG 1 cut(s) 163
SetI ASST 5 cut(s) 250, 273, 338, 381, 439
SfaNI GCATC 2 cut(s) 78, 144
Sfr274I CTCGAG 1 cut(s) 107
SinI GGWCC 2 cut(s) 125, 220
SlaI CTCGAG 1 cut(s) 107
SmiMI CAYNNNNRTG 3 cut(s) 86, 133, 329
SmlI CTYRAG 2 cut(s) 107, 206
SmoI CTYRAG 2 cut(s) 107, 206
Sse9I AATT 1 cut(s) 33
SsiI CCGC 5 cut(s) 22, 131, 212, 233, 403
SspMI CTAG 2 cut(s) 59, 92
StyD4I CCNGG 1 cut(s) 161
TaaI ACNGT 3 cut(s) 85, 224, 280
TaiI ACGT 1 cut(s) 338
TaqI TCGA 2 cut(s) 108, 170
TasI AATT 1 cut(s) 33
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 1 cut(s) 164
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 125, 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XhoI CTCGAG 1 cut(s) 107
XspI CTAG 2 cut(s) 59, 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.