Rw5G043810

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017635
Physical Location & Seq
Forward (+)
76872645 .. 76873288
644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043810.1

Sequence Viewer

Length: 447 bp
ATGATGCCGGAAAATCCCCAATCCCACCACAGAAAAATTGTGAAGACCCTCTACAAAACCCTAGCACACAGAGACACCAGAACCACCGTAATGCTAGTGGCTCCAGATCTCGAGTGGTGGTTCCACGGACCGCCGCAGTGCCAGCACATGATGAAGATGCTAACGGGGCAGTCGAGGCACTTGGAGTTCAAGTTCCAGCCCCGAAGTCTCAAGGCGGTCGGGGACCGTGTGGTGGCCGAGGGATGGGAAGGTAAGAAGGCATATTGGGTACAGGTGTGGACTGTTAACAAGGATGGGATGATCACGCAGTTGCGCGAGTATTTCAACACTTGGGTGACGCTGATGGTTAGGGTTTCGGAGGCCGAGGACGAGATGGTGAAGCTGTGGCAGAGTGATTCAAAGGAGCGGTTCCGGCGCTCGTTGCCGGATATTGTGTTAGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WI12

Protein Analysis

148

Amino Acids

17.58

Weight (kDa)

9.48

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 51 - 148 5e-27 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g39910
prunus_persica Prupe.6G046300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071881
rosa_laevigata RLG00000036259
rosa_multiflora Rmu_sc0002149.1_g000007 Rmu_sc0015603.1_g000001
rosa_roxburghii Rroxscaffold_1G00009280
rosa_rugosa Rorug05G0415300
rosa_samantha Rh5BG491300 Rh5CG515200 Rh5DG502800
rosa_wichuraiana Rw5G043810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 406
AccII CGCG 1 cut(s) 315
AciI CCGC 4 cut(s) 131, 134, 215, 406
AcoI YGGCCR 1 cut(s) 234
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 2 cut(s) 232, 243
AgsI TTSAA 3 cut(s) 190, 325, 399
AleI CACNNNNGTG 1 cut(s) 332
AloI GAACNNNNNNTCC 2 cut(s) 176, 208
AluBI AGCT 2 cut(s) 382, 440
AluI AGCT 2 cut(s) 382, 440
Alw26I GTCTC 2 cut(s) 66, 212
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 2 cut(s) 234, 360
AspLEI GCGC 2 cut(s) 315, 417
AspS9I GGNCC 2 cut(s) 128, 223
AsuHPI GGTGA 2 cut(s) 346, 388
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 2 cut(s) 128, 223
BaeI ACNNNNGTAYC 2 cut(s) 260, 293
BarI GAAGNNNNNNTAC 2 cut(s) 35, 67
BbsI GAAGAC 1 cut(s) 50
BccI CCATC 4 cut(s) 237, 287, 337, 367
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 66, 212
BfaI CTAG 2 cut(s) 62, 95
BfoI RGCGCY 1 cut(s) 418
BglII AGATCT 1 cut(s) 106
BisI GCNGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 135
Bme18I GGWCC 2 cut(s) 128, 223
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 128, 223
BmiI GGNNCC 4 cut(s) 102, 122, 224, 410
BmsI GCATC 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 194
BsaJI CCNNGG 3 cut(s) 124, 237, 363
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 243
BseDI CCNNGG 3 cut(s) 124, 237, 363
BseGI GGATG 3 cut(s) 248, 298, 303
BseLI CCNNNNNNNGG 2 cut(s) 232, 243
Bsh1236I CGCG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 236, 362
BsiEI CGRYCG 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 3 cut(s) 8, 412, 425
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 2 cut(s) 232, 243
BsmAI GTCTC 2 cut(s) 66, 212
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 2 cut(s) 236, 362
BsoBI CYCGRG 1 cut(s) 110
Bsp143I GATC 2 cut(s) 106, 300
BspACI CCGC 4 cut(s) 131, 134, 215, 406
BspANI GGCC 2 cut(s) 236, 362
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 4 cut(s) 102, 122, 224, 410
BsrBI CCGCTC 1 cut(s) 406
BssECI CCNNGG 3 cut(s) 124, 237, 363
BssMI GATC 2 cut(s) 106, 300
Bst4CI ACNGT 3 cut(s) 88, 227, 283
BstC8I GCNNGC 1 cut(s) 143
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 3 cut(s) 248, 298, 303
BstFNI CGCG 1 cut(s) 315
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 2 cut(s) 315, 417
BstKTI GATC 2 cut(s) 109, 303
BstMAI GTCTC 2 cut(s) 66, 212
BstMBI GATC 2 cut(s) 106, 300
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 5 cut(s) 142, 166, 175, 412, 421
BstUI CGCG 1 cut(s) 315
BstV2I GAAGAC 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BsuRI GGCC 2 cut(s) 236, 362
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 3 cut(s) 248, 298, 303
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 143
CfoI GCGC 2 cut(s) 315, 417
Cfr13I GGNCC 2 cut(s) 128, 223
CpoI CGGWCCG 1 cut(s) 128
CseI GACGC 1 cut(s) 346
Csp6I GTAC 1 cut(s) 269
CspI CGGWCCG 1 cut(s) 128
CviAII CATG 1 cut(s) 148
CviJI RGCY 6 cut(s) 101, 199, 236, 362, 382, 440
CviKI_1 RGCY 6 cut(s) 101, 199, 236, 362, 382, 440
CviQI GTAC 1 cut(s) 269
DpnI GATC 2 cut(s) 108, 302
DpnII GATC 2 cut(s) 106, 300
EaeI YGGCCR 1 cut(s) 234
Eco47I GGWCC 2 cut(s) 128, 223
Eco88I CYCGRG 1 cut(s) 110
FaeI CATG 1 cut(s) 151
FaiI YATR 2 cut(s) 149, 262
FaqI GGGAC 1 cut(s) 236
FatI CATG 1 cut(s) 147
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 134
FokI GGATG 3 cut(s) 255, 305, 310
Fsp4HI GCNGC 1 cut(s) 134
FspBI CTAG 2 cut(s) 62, 95
GlaI GCGC 2 cut(s) 314, 416
GluI GCNGC 1 cut(s) 134
GsuI CTGGAG 1 cut(s) 87
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 2 cut(s) 236, 362
HapII CCGG 3 cut(s) 8, 412, 425
HgaI GACGC 1 cut(s) 346
HhaI GCGC 2 cut(s) 315, 417
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 2 cut(s) 313, 415
HinP1I GCGC 2 cut(s) 313, 415
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 395
HpaI GTTAAC 1 cut(s) 286
HpaII CCGG 3 cut(s) 8, 412, 425
HphI GGTGA 2 cut(s) 346, 388
Hpy166II GTNNAC 2 cut(s) 279, 286
Hpy188I TCNGA 2 cut(s) 358, 446
Hpy188III TCNNGA 2 cut(s) 104, 110
Hpy8I GTNNAC 2 cut(s) 279, 286
HpyAV CCTTC 2 cut(s) 242, 250
HpyCH4III ACNGT 3 cut(s) 88, 227, 283
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 166, 175, 412, 421
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 2 cut(s) 313, 415
Ksp22I TGATCA 1 cut(s) 300
KspAI GTTAAC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 106, 300
LmnI GCTCC 2 cut(s) 106, 403
LpnPI CCDG 8 cut(s) 21, 91, 117, 155, 209, 257, 425, 438
LweI GCATC 1 cut(s) 147
MaeI CTAG 2 cut(s) 62, 95
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 108, 302
MbiI CCGCTC 1 cut(s) 406
MboI GATC 2 cut(s) 106, 300
MboII GAAGA 2 cut(s) 55, 166
MflI RGATCY 1 cut(s) 106
MluCI AATT 1 cut(s) 36
MnlI CCTC 5 cut(s) 59, 168, 232, 352, 358
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 2 cut(s) 89, 332
MspI CCGG 3 cut(s) 8, 412, 425
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 5 cut(s) 142, 166, 175, 412, 421
NdeII GATC 2 cut(s) 106, 300
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 4 cut(s) 102, 122, 224, 410
NmeAIII GCCGAG 2 cut(s) 262, 388
NmuCI GTSAC 1 cut(s) 334
OliI CACNNNNGTG 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 110
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PfeI GAWTC 1 cut(s) 395
PkrI GCNGC 1 cut(s) 135
PspN4I GGNNCC 4 cut(s) 102, 122, 224, 410
PspPI GGNCC 2 cut(s) 128, 223
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
RseI CAYNNNNRTG 2 cut(s) 89, 332
Rsr2I CGGWCCG 1 cut(s) 128
RsrII CGGWCCG 1 cut(s) 128
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 134
Sau3AI GATC 2 cut(s) 106, 300
Sau96I GGNCC 2 cut(s) 128, 223
SetI ASST 4 cut(s) 253, 276, 384, 442
SfaNI GCATC 1 cut(s) 147
Sfr274I CTCGAG 1 cut(s) 110
SinI GGWCC 2 cut(s) 128, 223
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 89, 332
SmlI CTYRAG 2 cut(s) 110, 209
SmoI CTYRAG 2 cut(s) 110, 209
Sse9I AATT 1 cut(s) 36
SsiI CCGC 4 cut(s) 131, 134, 215, 406
SspMI CTAG 2 cut(s) 62, 95
TaaI ACNGT 3 cut(s) 88, 227, 283
TaqI TCGA 2 cut(s) 111, 173
TasI AATT 1 cut(s) 36
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 128, 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 2 cut(s) 62, 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.