Rmu_sc0000253.1_g000019

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000253.1
OG0015197
Physical Location & Seq
Reverse (-)
65697 .. 68871
3175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000253.1_g000019.1.cds

Sequence Viewer

Length: 1443 bp
atgtcctcaccgtccctccgcgtctccaccaccgcccaccgccacttggggttcaaccgctccctctccccctcaacaccccaaaacctcttcacttcactctccttccccacttccactttcccaaaacaccctcggaagctcgccgcccggtgccaaaagaagcaaggcaaagccaatgaagcggggtcgatcaaggtcaagggcaaaccgggaaatgtgtggagcgtggacaacgagctggctgctcaagagaaagggaagcccacaaagagaagagggagaaggagagttgtgaaggggaagagaagtagaggtggtggtggcagagctgttctggtctctggtgctatgttaatggaggtcgagactgttcttcaaactcaggaacctgtgataagaccctcatggaatacattttctagtagtgtcagtgggatatggaagggtgttggagcagttttttcacccatcactgcagaaatggagccaattgattttggaagcagggatgaacaactttatgattgctatactctttctcgcattgaggctgtgccatcatcgtctggagggccagcatcacatgtccaaaggaaaattaattgggtgactttaaatcctcatggtgaaaaaagcaatagtaatgaaagtggaagaaatcatgtgttgcctcaatttgaggcctttaactttgaaagaagtgacgtgatggaagaagatgtcatgggcagagaacctggtcttgttttctttgaggatggatcttattctaggggtcctgttgatatcccagttggtgaagttgatgattctaactattacctttcaccaactttcaaatttgaacagtgcttggttaaaggttgccacaagagacttcgaattgttcataccatagaattcagcaatggtggttcaaacattcagataatgagagttgccatgtatgaggaacagtgggttagccctgtcaatcttctggaccaaagtgattcggagcttgatttgaaaccattttctcagcggaaacgaactcagtcatcagagctggctgggccatggaaggtgtttgaggtcagtgctactccaatttatggtgaggagatggaccagatggtgttggagcaacagaatagcgttccctatgtctacctttgcacagaggctttaaagaaaaggagcttgcctgggcacgctgcttactttggtgaggaagagatgctagacatgcaagatgcgactatgctttggcttccaggtggtgtcactgcctatgttgatgtaaataaggatggcatcctttgtattggagttgggtggtactcagatgaaggaatcaaccttgttatggagagggattatggaatagatgggaaactcaaggacatccgatcgaagtcagaaatgaaaagaaggtggtcggattctcagcccgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

480

Amino Acids

53.63

Weight (kDa)

7.12

Isoelectric Point (pI)

57.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44760
fragaria_vesca FvH4_7g10380
malus_domestica MD07G1109900.v1.1
prunus_persica Prupe.2G133500_v2.0.a1
pyrus_communis pycom07g09470
rosa_chinensis RchiOBHm_Chr1g0348081
rosa_laevigata RLG00000028692
rosa_multiflora Rmu_sc0000253.1_g000019 Rmu_sc0002523.1_g000001
rosa_roxburghii Rroxscaffold_4G00307250
rosa_rugosa Rorug01G0192200
rosa_samantha Rh1AG209900 Rh1BG175500 Rh1CG194300 Rh1DG205300
rosa_wichuraiana Rw1G017510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 153
AccB7I CCANNNNNTGG 1 cut(s) 1097
AccBSI CCGCTC 1 cut(s) 60
AccI GTMKAC 1 cut(s) 1152
AccII CGCG 1 cut(s) 21
AciI CCGC 7 cut(s) 19, 33, 40, 58, 147, 185, 1027
AclWI GGATC 1 cut(s) 772
AcsI RAATTY 2 cut(s) 842, 902
AfaI GTAC 1 cut(s) 1325
AfiI CCNNNNNNNGG 3 cut(s) 46, 1097, 1351
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 7 cut(s) 55, 380, 698, 841, 848, 921, 1012
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 3 cut(s) 739, 1189, 1258
AluBI AGCT 6 cut(s) 142, 241, 332, 1003, 1051, 1185
AluI AGCT 6 cut(s) 142, 241, 332, 1003, 1051, 1185
Alw26I GTCTC 4 cut(s) 28, 346, 362, 871
AlwI GGATC 1 cut(s) 772
Ama87I CYCGRG 1 cut(s) 1436
AoxI GGCC 3 cut(s) 575, 684, 1058
ApeKI GCWGC 2 cut(s) 245, 1199
ApoI RAATTY 2 cut(s) 842, 902
AseI ATTAAT 1 cut(s) 603
AspS9I GGNCC 5 cut(s) 575, 779, 985, 1058, 1111
AsuC2I CCSGG 2 cut(s) 151, 213
AsuHPI GGTGA 7 cut(s) 459, 622, 641, 812, 822, 1112, 1223
AsuII TTCGAA 1 cut(s) 883
AvaI CYCGRG 1 cut(s) 1436
AvaII GGWCC 3 cut(s) 779, 985, 1111
BaeGI GKGCMC 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 153
BbvI GCAGC 2 cut(s) 232, 1186
BccI CCATC 8 cut(s) 479, 568, 706, 755, 1102, 1111, 1289, 1367
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 3 cut(s) 741, 1191, 1260
BcnI CCSGG 2 cut(s) 151, 213
BcoDI GTCTC 4 cut(s) 28, 346, 362, 871
BfaI CTAG 3 cut(s) 423, 774, 1226
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 3 cut(s) 147, 246, 1200
BlsI GCNGC 3 cut(s) 148, 247, 1201
Bme1390I CCNGG 5 cut(s) 151, 213, 741, 1191, 1260
Bme18I GGWCC 3 cut(s) 779, 985, 1111
BmeT110I CYCGRG 1 cut(s) 1436
BmgBI CACGTC 1 cut(s) 709
BmgT120I GGNCC 5 cut(s) 575, 779, 985, 1058, 1111
BmiI GGNNCC 4 cut(s) 155, 390, 489, 780
BmrFI CCNGG 5 cut(s) 151, 213, 741, 1191, 1260
BmrI ACTGGG 1 cut(s) 788
BmsI GCATC 4 cut(s) 590, 1212, 1228, 1308
BmuI ACTGGG 1 cut(s) 788
BpmI CTGGAG 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 883
BpuEI CTTGAG 2 cut(s) 234, 1367
BpuMI CCSGG 2 cut(s) 151, 213
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 3 cut(s) 134, 1061, 1190
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 1097, 1351
Bse1I ACTGG 1 cut(s) 794
Bse3DI GCAATG 1 cut(s) 916
BseBI CCWGG 3 cut(s) 741, 1191, 1260
BseDI CCNNGG 3 cut(s) 134, 1061, 1190
BseGI GGATG 5 cut(s) 517, 766, 1299, 1300, 1389
BseLI CCNNNNNNNGG 3 cut(s) 46, 1097, 1351
BseMI GCAATG 1 cut(s) 916
BseMII CTCAG 4 cut(s) 398, 1037, 1052, 1341
BseNI ACTGG 1 cut(s) 794
BseRI GAGGAG 1 cut(s) 1118
BseSI GKGCMC 1 cut(s) 1197
BseXI GCAGC 2 cut(s) 232, 1186
BseYI CCCAGC 1 cut(s) 1055
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 1397
BshFI GGCC 3 cut(s) 577, 686, 1060
BshNI GGYRCC 1 cut(s) 153
BsiEI CGRYCG 1 cut(s) 1397
BsiHKCI CYCGRG 1 cut(s) 1436
BsiSI CCGG 2 cut(s) 151, 212
BslI CCNNNNNNNGG 3 cut(s) 46, 1097, 1351
BsmAI GTCTC 4 cut(s) 28, 346, 362, 871
BsmBI CGTCTC 1 cut(s) 28
BsnI GGCC 3 cut(s) 577, 686, 1060
Bso31I GGTCTC 1 cut(s) 346
BsoBI CYCGRG 1 cut(s) 1436
Bsp119I TTCGAA 1 cut(s) 883
Bsp1286I GDGCHC 1 cut(s) 1197
Bsp143I GATC 3 cut(s) 192, 764, 1394
Bsp19I CCATGG 1 cut(s) 1061
BspACI CCGC 7 cut(s) 19, 33, 40, 58, 147, 185, 1027
BspANI GGCC 3 cut(s) 577, 686, 1060
BspCNI CTCAG 4 cut(s) 397, 1036, 1051, 1340
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 4 cut(s) 155, 390, 489, 780
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 1 cut(s) 772
BspT104I TTCGAA 1 cut(s) 883
BspT107I GGYRCC 1 cut(s) 153
BspTNI GGTCTC 1 cut(s) 346
BsrBI CCGCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 916
BsrI ACTGG 1 cut(s) 794
BssECI CCNNGG 3 cut(s) 134, 1061, 1190
BssMI GATC 3 cut(s) 192, 764, 1394
BssT1I CCWWGG 1 cut(s) 1061
Bst2UI CCWGG 3 cut(s) 741, 1191, 1260
Bst4CI ACNGT 4 cut(s) 12, 373, 852, 960
Bst6I CTCTTC 4 cut(s) 95, 271, 299, 1212
BstBI TTCGAA 1 cut(s) 883
BstC8I GCNNGC 6 cut(s) 144, 243, 579, 1053, 1187, 1197
BstDEI CTNAG 5 cut(s) 384, 1023, 1038, 1327, 1431
BstDSI CCRYGG 1 cut(s) 1061
BstF5I GGATG 5 cut(s) 517, 766, 1299, 1300, 1389
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 3 cut(s) 195, 767, 1397
BstMAI GTCTC 4 cut(s) 28, 346, 362, 871
BstMBI GATC 3 cut(s) 192, 764, 1394
BstMCI CGRYCG 1 cut(s) 1397
BstMWI GCNNNNNNNGC 3 cut(s) 182, 1057, 1231
BstNI CCWGG 3 cut(s) 741, 1191, 1260
BstNSI RCATGY 2 cut(s) 590, 1234
BstSCI CCNGG 5 cut(s) 149, 211, 739, 1189, 1258
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 1197
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 232, 1186
BstX2I RGATCY 1 cut(s) 764
BstYI RGATCY 1 cut(s) 764
BsuRI GGCC 3 cut(s) 577, 686, 1060
BtgI CCRYGG 1 cut(s) 1061
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 5 cut(s) 517, 766, 1299, 1300, 1389
BtsI GCAGTG 2 cut(s) 474, 1269
BtsIMutI CAGTG 6 cut(s) 439, 474, 857, 965, 1087, 1269
Cac8I GCNNGC 6 cut(s) 144, 243, 579, 1053, 1187, 1197
Cfr13I GGNCC 5 cut(s) 575, 779, 985, 1058, 1111
CseI GACGC 1 cut(s) 10
CsiI ACCWGGT 1 cut(s) 739
Csp6I GTAC 1 cut(s) 1324
CviAII CATG 8 cut(s) 408, 587, 626, 665, 727, 946, 1062, 1231
CviQI GTAC 1 cut(s) 1324
DdeI CTNAG 5 cut(s) 384, 1023, 1038, 1327, 1431
DpnI GATC 3 cut(s) 194, 766, 1396
DpnII GATC 3 cut(s) 192, 764, 1394
DraI TTTAAA 2 cut(s) 618, 1173
Eam1104I CTCTTC 4 cut(s) 95, 271, 299, 1212
EarI CTCTTC 4 cut(s) 95, 271, 299, 1212
Eco130I CCWWGG 1 cut(s) 1061
Eco147I AGGCCT 1 cut(s) 686
Eco31I GGTCTC 1 cut(s) 346
Eco32I GATATC 1 cut(s) 790
Eco47I GGWCC 3 cut(s) 779, 985, 1111
Eco88I CYCGRG 1 cut(s) 1436
EcoO109I RGGNCCY 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 902
EcoRII CCWGG 3 cut(s) 739, 1189, 1258
EcoRV GATATC 1 cut(s) 790
EcoT14I CCWWGG 1 cut(s) 1061
ErhI CCWWGG 1 cut(s) 1061
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 8 cut(s) 411, 590, 629, 668, 730, 949, 1065, 1234
FatI CATG 8 cut(s) 407, 586, 625, 664, 726, 945, 1061, 1230
FauI CCCGC 1 cut(s) 178
FblI GTMKAC 1 cut(s) 1152
Fnu4HI GCNGC 3 cut(s) 147, 246, 1200
FokI GGATG 5 cut(s) 524, 773, 1286, 1307, 1376
Fsp4HI GCNGC 3 cut(s) 147, 246, 1200
FspBI CTAG 3 cut(s) 423, 774, 1226
GluI GCNGC 3 cut(s) 147, 246, 1200
GsaI CCCAGC 1 cut(s) 1059
GsuI CTGGAG 1 cut(s) 591
HaeIII GGCC 3 cut(s) 577, 686, 1060
HapII CCGG 2 cut(s) 151, 212
HgaI GACGC 1 cut(s) 10
Hin1II CATG 8 cut(s) 411, 590, 629, 668, 730, 949, 1065, 1234
HinfI GANTC 4 cut(s) 812, 995, 1338, 1427
HpaII CCGG 2 cut(s) 151, 212
HphI GGTGA 7 cut(s) 459, 622, 641, 812, 822, 1112, 1223
Hpy166II GTNNAC 2 cut(s) 232, 1153
Hpy188I TCNGA 8 cut(s) 138, 930, 1000, 1048, 1330, 1394, 1405, 1426
Hpy188III TCNNGA 5 cut(s) 251, 367, 386, 570, 983
Hpy8I GTNNAC 2 cut(s) 232, 1153
HpyAV CCTTC 7 cut(s) 115, 279, 292, 439, 1060, 1328, 1410
HpyCH4III ACNGT 4 cut(s) 12, 373, 852, 960
HpyCH4IV ACGT 1 cut(s) 708
HpyCH4V TGCA 3 cut(s) 479, 1161, 1234
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 1057, 1231
HpyF3I CTNAG 5 cut(s) 384, 1023, 1038, 1327, 1431
HpySE526I ACGT 1 cut(s) 708
Hsp92II CATG 8 cut(s) 411, 590, 629, 668, 730, 949, 1065, 1234
Kzo9I GATC 3 cut(s) 192, 764, 1394
LmnI GCTCC 7 cut(s) 65, 225, 455, 487, 1000, 1126, 1182
Lsp1109I GCAGC 2 cut(s) 232, 1186
LweI GCATC 4 cut(s) 590, 1212, 1228, 1308
MabI ACCWGGT 1 cut(s) 739
MaeI CTAG 3 cut(s) 423, 774, 1226
MaeII ACGT 1 cut(s) 708
MaeIII GTNAC 3 cut(s) 610, 704, 1267
MalI GATC 3 cut(s) 194, 766, 1396
MbiI CCGCTC 1 cut(s) 60
MboI GATC 3 cut(s) 192, 764, 1394
MboII GAAGA 9 cut(s) 82, 288, 316, 368, 669, 728, 731, 971, 1229
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 1 cut(s) 764
MhlI GDGCHC 1 cut(s) 1197
MluCI AATT 8 cut(s) 492, 600, 604, 677, 842, 885, 902, 1092
MmeI TCCRAC 3 cut(s) 433, 1104, 1404
MseI TTAA 6 cut(s) 356, 603, 617, 690, 861, 1172
MspA1I CMGCKG 1 cut(s) 1027
MspI CCGG 2 cut(s) 151, 212
MspR9I CCNGG 5 cut(s) 151, 213, 741, 1191, 1260
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 3 cut(s) 741, 1191, 1260
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 182, 1057, 1231
NciI CCSGG 2 cut(s) 151, 213
NcoI CCATGG 1 cut(s) 1061
NdeII GATC 3 cut(s) 192, 764, 1394
NlaIII CATG 8 cut(s) 411, 590, 629, 668, 730, 949, 1065, 1234
NlaIV GGNNCC 4 cut(s) 155, 390, 489, 780
NmuCI GTSAC 3 cut(s) 610, 704, 1267
NspI RCATGY 2 cut(s) 590, 1234
NspV TTCGAA 1 cut(s) 883
PceI AGGCCT 1 cut(s) 686
PciI ACATGT 1 cut(s) 586
PfeI GAWTC 4 cut(s) 812, 995, 1338, 1427
PflMI CCANNNNNTGG 1 cut(s) 1097
PkrI GCNGC 3 cut(s) 148, 247, 1201
Ple19I CGATCG 1 cut(s) 1397
PpuMI RGGWCCY 1 cut(s) 779
PscI ACATGT 1 cut(s) 586
PshBI ATTAAT 1 cut(s) 603
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 3 cut(s) 739, 1189, 1258
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 3 cut(s) 739, 1189, 1258
PspN4I GGNNCC 4 cut(s) 155, 390, 489, 780
PspPI GGNCC 5 cut(s) 575, 779, 985, 1058, 1111
PspPPI RGGWCCY 1 cut(s) 779
PstI CTGCAG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 764
PvuI CGATCG 1 cut(s) 1397
RsaI GTAC 1 cut(s) 1325
RsaNI GTAC 1 cut(s) 1324
SaqAI TTAA 6 cut(s) 356, 603, 617, 690, 861, 1172
SatI GCNGC 3 cut(s) 147, 246, 1200
Sau3AI GATC 3 cut(s) 192, 764, 1394
Sau96I GGNCC 5 cut(s) 575, 779, 985, 1058, 1111
ScrFI CCNGG 5 cut(s) 151, 213, 741, 1191, 1260
SduI GDGCHC 1 cut(s) 1197
SexAI ACCWGGT 1 cut(s) 739
SfaNI GCATC 4 cut(s) 590, 1212, 1228, 1308
SfcI CTRYAG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 883
SinI GGWCC 3 cut(s) 779, 985, 1111
SmlI CTYRAG 2 cut(s) 249, 1382
SmoI CTYRAG 2 cut(s) 249, 1382
Sse9I AATT 8 cut(s) 492, 600, 604, 677, 842, 885, 902, 1092
SseBI AGGCCT 1 cut(s) 686
SsiI CCGC 7 cut(s) 19, 33, 40, 58, 147, 185, 1027
SspMI CTAG 3 cut(s) 423, 774, 1226
StuI AGGCCT 1 cut(s) 686
StyD4I CCNGG 5 cut(s) 149, 211, 739, 1189, 1258
StyI CCWWGG 1 cut(s) 1061
TaaI ACNGT 4 cut(s) 12, 373, 852, 960
TaiI ACGT 1 cut(s) 711
TaqI TCGA 4 cut(s) 191, 366, 883, 1397
TasI AATT 8 cut(s) 492, 600, 604, 677, 842, 885, 902, 1092
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 4 cut(s) 812, 995, 1338, 1427
Tru1I TTAA 6 cut(s) 356, 603, 617, 690, 861, 1172
Tru9I TTAA 6 cut(s) 356, 603, 617, 690, 861, 1172
TscAI CASTG 6 cut(s) 439, 481, 857, 965, 1087, 1276
TseFI GTSAC 3 cut(s) 610, 704, 1267
TseI GCWGC 2 cut(s) 245, 1199
Tsp45I GTSAC 3 cut(s) 610, 704, 1267
TspDTI ATGAA 6 cut(s) 195, 528, 663, 881, 1347, 1424
TspRI CASTG 6 cut(s) 439, 481, 857, 965, 1087, 1276
Van91I CCANNNNNTGG 1 cut(s) 1097
VpaK11BI GGWCC 3 cut(s) 779, 985, 1111
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 2 cut(s) 842, 902
XceI RCATGY 2 cut(s) 590, 1234
XmiI GTMKAC 1 cut(s) 1152
XspI CTAG 3 cut(s) 423, 774, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.