Rmu_sc0000301.1_g000002

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000301.1
OG0018655
Physical Location & Seq
Reverse (-)
15933 .. 16274
342 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 342 bp
ctgcattttcaccaccaccgccctttcacaaccaaccagtgctccgccgtactctcaagaacccatcgacgccgccagccgatctctcctcctcctccttcaatctccccaaccccaccactggcttcaatcccacccaagactcctctcgtccctgccctcttcgtcatcggagactcctacagcaccaacaatttcctcgggacattggcacgcaccgacagcctcccctacggctgcgatttcgacacgcacgagcccactgggcggttctgcaatggcagaatcccaatcaattatattggtaatttcgcttctttagaacacccttttcttgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

113

Amino Acids

12.56

Weight (kDa)

8.64

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 19, 45, 73, 268
AcyI GRCGYC 1 cut(s) 70
AfaI GTAC 1 cut(s) 51
AfiI CCNNNNNNNGG 2 cut(s) 121, 267
AgsI TTSAA 2 cut(s) 102, 129
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 200
ApeKI GCWGC 1 cut(s) 237
AvaI CYCGRG 1 cut(s) 200
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 298, 330
BauI CACGAG 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 224
BccI CCATC 1 cut(s) 72
BceAI ACGGC 2 cut(s) 32, 250
BcoDI GTCTC 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 181
BglI GCCNNNNNGGC 1 cut(s) 265
BisI GCNGC 2 cut(s) 73, 238
BlsI GCNGC 2 cut(s) 74, 239
BmeT110I CYCGRG 1 cut(s) 200
BmrI ACTGGG 1 cut(s) 273
BmuI ACTGGG 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 40
BsaHI GRCGYC 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 267
Bse1I ACTGG 3 cut(s) 37, 126, 268
Bse3DI GCAATG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 199
BseLI CCNNNNNNNGG 2 cut(s) 121, 267
BseMI GCAATG 1 cut(s) 283
BseNI ACTGG 3 cut(s) 37, 126, 268
BseRI GAGGAG 4 cut(s) 78, 81, 84, 135
BseXI GCAGC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 200
BslFI GGGAC 2 cut(s) 137, 217
BslI CCNNNNNNNGG 2 cut(s) 121, 267
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 2 cut(s) 137, 217
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 44, 261
Bsp143I GATC 1 cut(s) 81
BspACI CCGC 4 cut(s) 19, 45, 73, 268
BsrDI GCAATG 1 cut(s) 283
BsrI ACTGG 3 cut(s) 37, 126, 268
BssECI CCNNGG 1 cut(s) 199
BssMI GATC 1 cut(s) 81
BssNI GRCGYC 1 cut(s) 70
BssSI CACGAG 1 cut(s) 254
Bst2BI CACGAG 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 70
BstC8I GCNNGC 2 cut(s) 77, 214
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 2 cut(s) 222, 265
BstSFI CTRYAG 1 cut(s) 181
BstV1I GCAGC 1 cut(s) 224
BtsIMutI CAGTG 3 cut(s) 44, 119, 261
Cac8I GCNNGC 2 cut(s) 77, 214
CseI GACGC 1 cut(s) 78
Csp6I GTAC 1 cut(s) 50
CviJI RGCY 5 cut(s) 79, 125, 225, 237, 259
CviKI_1 RGCY 5 cut(s) 79, 125, 225, 237, 259
CviQI GTAC 1 cut(s) 50
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 200
EcoT38I GRGCYC 1 cut(s) 261
FaiI YATR 1 cut(s) 300
FaqI GGGAC 2 cut(s) 137, 217
Fnu4HI GCNGC 2 cut(s) 73, 238
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 2 cut(s) 73, 238
GluI GCNGC 2 cut(s) 73, 238
HgaI GACGC 1 cut(s) 78
Hin1I GRCGYC 1 cut(s) 70
HinfI GANTC 3 cut(s) 142, 176, 285
Hpy188I TCNGA 1 cut(s) 173
Hpy188III TCNNGA 3 cut(s) 57, 202, 335
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 1 cut(s) 108
HpyCH4V TGCA 2 cut(s) 4, 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 222, 265
Hsp92I GRCGYC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 5 cut(s) 50, 89, 107, 168, 249
Lsp1109I GCAGC 1 cut(s) 224
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MboII GAAGA 1 cut(s) 154
MhlI GDGCHC 2 cut(s) 44, 261
MluCI AATT 3 cut(s) 193, 295, 307
MlyI GAGTC 2 cut(s) 136, 170
MnlI CCTC 7 cut(s) 99, 102, 105, 156, 170, 209, 236
MwoI GCNNNNNNNGC 2 cut(s) 222, 265
NdeII GATC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 1 cut(s) 285
PkrI GCNGC 2 cut(s) 74, 239
PleI GAGTC 2 cut(s) 136, 170
PpsI GAGTC 2 cut(s) 136, 170
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
SatI GCNGC 2 cut(s) 73, 238
Sau3AI GATC 1 cut(s) 81
SchI GAGTC 2 cut(s) 136, 170
SduI GDGCHC 2 cut(s) 44, 261
SfcI CTRYAG 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
Sse9I AATT 3 cut(s) 193, 295, 307
SsiI CCGC 4 cut(s) 19, 45, 73, 268
TaqI TCGA 2 cut(s) 67, 246
TasI AATT 3 cut(s) 193, 295, 307
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 1 cut(s) 285
TscAI CASTG 3 cut(s) 44, 126, 268
TseI GCWGC 1 cut(s) 237
TspRI CASTG 3 cut(s) 44, 126, 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.