Rmu_sc0001616.1_g000025

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001616.1
OG0018655
Physical Location & Seq
Forward (+)
136368 .. 136736
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001616.1_g000025.1.cds

Sequence Viewer

Length: 369 bp
atgatcaatcttcttctctcttttctctgcattttcaccaccactgccctttcacaaccaaccagtactccgccgtactctcaagaatccatcgacgccgccacccctatctctcctgctcctccttcaatctccccaaccccaccactggcttcaatcccacccaagactcctctcgtccctgccctcttcatcatcggagactcctacggcaccaacaatttcctcgggacattggcacgtgctgacagcctcccctatggccgcgatttcgacacgcacgagcccactgggcagttctgcaatggtagaatcccagtcgattatattggtaatttcgcttctttagaataccattttcttgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

122

Amino Acids

13.12

Weight (kDa)

4.45

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AccII CGCG 1 cut(s) 267
AciI CCGC 3 cut(s) 71, 99, 265
AcoI YGGCCR 1 cut(s) 262
AcvI CACGTG 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 2 cut(s) 67, 77
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 2 cut(s) 129, 156
Alw26I GTCTC 1 cut(s) 195
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 227
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 325, 357
BauI CACGAG 1 cut(s) 281
BbrPI CACGTG 1 cut(s) 242
BccI CCATC 1 cut(s) 98
BceAI ACGGC 2 cut(s) 58, 226
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 195
BglI GCCNNNNNGGC 1 cut(s) 292
BisI GCNGC 2 cut(s) 99, 265
BlsI GCNGC 2 cut(s) 100, 266
BmcAI AGTACT 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 214
BmrI ACTGGG 2 cut(s) 300, 311
BmuI ACTGGG 2 cut(s) 300, 311
BpuEI CTTGAG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse1I ACTGG 4 cut(s) 63, 153, 295, 317
Bse3DI GCAATG 1 cut(s) 310
BseDI CCNNGG 1 cut(s) 226
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMI GCAATG 1 cut(s) 310
BseNI ACTGG 4 cut(s) 63, 153, 295, 317
BseRI GAGGAG 2 cut(s) 111, 162
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 227
BslFI GGGAC 2 cut(s) 164, 244
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 1 cut(s) 195
BsmFI GGGAC 2 cut(s) 164, 244
BsnI GGCC 1 cut(s) 264
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 3 cut(s) 71, 99, 265
BspANI GGCC 1 cut(s) 264
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 310
BsrI ACTGG 4 cut(s) 63, 153, 295, 317
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 1 cut(s) 3
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 194
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 242
BstFNI CGCG 1 cut(s) 267
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstUI CGCG 1 cut(s) 267
BsuRI GGCC 1 cut(s) 264
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 3 cut(s) 42, 146, 288
CseI GACGC 1 cut(s) 104
Csp6I GTAC 2 cut(s) 66, 76
CviJI RGCY 4 cut(s) 152, 252, 264, 286
CviKI_1 RGCY 4 cut(s) 152, 252, 264, 286
CviQI GTAC 2 cut(s) 66, 76
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 288
Eco72I CACGTG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 288
FaiI YATR 2 cut(s) 261, 327
FaqI GGGAC 2 cut(s) 164, 244
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 99, 265
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 2 cut(s) 99, 265
GluI GCNGC 2 cut(s) 99, 265
HaeIII GGCC 1 cut(s) 264
HgaI GACGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 96
HinfI GANTC 4 cut(s) 86, 169, 203, 312
HphI GGTGA 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 3 cut(s) 83, 229, 362
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 135
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 2 cut(s) 30, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpySE526I ACGT 1 cut(s) 241
Hsp92I GRCGYC 1 cut(s) 96
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 6 cut(s) 76, 129, 134, 195, 276, 330
MaeII ACGT 1 cut(s) 241
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 2 cut(s) 5, 181
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 2 cut(s) 220, 334
MlyI GAGTC 2 cut(s) 163, 197
MnlI CCTC 5 cut(s) 132, 183, 197, 236, 263
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 292
NdeII GATC 1 cut(s) 3
NlaIV GGNNCC 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PfeI GAWTC 2 cut(s) 86, 312
PkrI GCNGC 2 cut(s) 100, 266
PleI GAGTC 2 cut(s) 163, 197
PmaCI CACGTG 1 cut(s) 242
PmlI CACGTG 1 cut(s) 242
PpsI GAGTC 2 cut(s) 163, 197
Ppu21I YACGTR 1 cut(s) 242
PspCI CACGTG 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 214
RsaI GTAC 2 cut(s) 67, 77
RsaNI GTAC 2 cut(s) 66, 76
SatI GCNGC 2 cut(s) 99, 265
Sau3AI GATC 1 cut(s) 3
ScaI AGTACT 1 cut(s) 67
SchI GAGTC 2 cut(s) 163, 197
SduI GDGCHC 1 cut(s) 288
SetI ASST 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 2 cut(s) 220, 334
SsiI CCGC 3 cut(s) 71, 99, 265
TaiI ACGT 1 cut(s) 244
TaqI TCGA 3 cut(s) 93, 273, 321
TasI AATT 2 cut(s) 220, 334
TatI WGTACW 1 cut(s) 65
TauI GCSGC 2 cut(s) 101, 267
TfiI GAWTC 2 cut(s) 86, 312
TscAI CASTG 3 cut(s) 49, 153, 295
TspDTI ATGAA 1 cut(s) 181
TspRI CASTG 3 cut(s) 49, 153, 295
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.