Rh5CG243400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0018655
Physical Location & Seq
Forward (+)
25327792 .. 25328175
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG243400.1

Sequence Viewer

Length: 384 bp
ATGGCGATGATCAATCTTCTTCTCTCTTTTCTCTGCATTTTCACCACCACCGCCCTTTCACAACCAACCAATGCTCCGCTGTACTCTCAAGAACCCATCGACGCCGCCACCCCCATCTCTCCTCCTCCTCCTTCAATCTCCCCAACCCCACCACCGGCTTCAATCCCACCCAAGACTCCTCTAGTCCCTACCCTCTTCGTCATCGGAGACTCCTCCGTCAACTCTGGCACCAACAATTTCCTCGGGACATTGGCACCGACCAACTGCCTCCCCTACGGCCGCGATTTCAACACGCACGAGCCCATTGGGAAGTTCTGCAATGGCAGAATCCCAGTCGATTATATTGGTAATTTCGCTTCTTTAGAATACCCTTTTCTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

127

Amino Acids

13.55

Weight (kDa)

4.55

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccB1I GGYRCC 2 cut(s) 227, 253
AccII CGCG 1 cut(s) 282
AciI CCGC 4 cut(s) 51, 77, 105, 280
AcoI YGGCCR 1 cut(s) 277
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 3 cut(s) 135, 162, 289
Alw26I GTCTC 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 1 cut(s) 277
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 242
BanI GGYRCC 2 cut(s) 227, 253
BanII GRGCYC 1 cut(s) 303
BarI GAAGNNNNNNTAC 2 cut(s) 340, 372
BauI CACGAG 1 cut(s) 296
BccI CCATC 2 cut(s) 104, 122
BceAI ACGGC 1 cut(s) 292
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 182
BisI GCNGC 2 cut(s) 105, 280
BlsI GCNGC 2 cut(s) 106, 281
BmeT110I CYCGRG 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 229, 255
BmrI ACTGGG 1 cut(s) 326
BmuI ACTGGG 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 1 cut(s) 325
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 5 cut(s) 111, 114, 117, 168, 202
BseX3I CGGCCG 1 cut(s) 277
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 1 cut(s) 279
BshNI GGYRCC 2 cut(s) 227, 253
BsiEI CGRYCG 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 1 cut(s) 155
BslFI GGGAC 2 cut(s) 170, 259
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 2 cut(s) 170, 259
BsnI GGCC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 1 cut(s) 9
BspACI CCGC 4 cut(s) 51, 77, 105, 280
BspANI GGCC 1 cut(s) 279
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 229, 255
BspT107I GGYRCC 2 cut(s) 227, 253
BsrDI GCAATG 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 1 cut(s) 9
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 296
Bst2BI CACGAG 1 cut(s) 296
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 102
BstFNI CGCG 1 cut(s) 282
BstKTI GATC 1 cut(s) 12
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 1 cut(s) 9
BstMCI CGRYCG 1 cut(s) 280
BstUI CGCG 1 cut(s) 282
BstZI CGGCCG 1 cut(s) 277
BsuRI GGCC 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 20
Cfr10I RCCGGY 1 cut(s) 154
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 82
CviJI RGCY 3 cut(s) 158, 279, 301
CviKI_1 RGCY 3 cut(s) 158, 279, 301
CviQI GTAC 1 cut(s) 82
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 277
EagI CGGCCG 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
EclXI CGGCCG 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 303
Eco52I CGGCCG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 303
FaiI YATR 1 cut(s) 342
FaqI GGGAC 2 cut(s) 170, 259
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 105, 280
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 2 cut(s) 105, 280
FspBI CTAG 1 cut(s) 182
GluI GCNGC 2 cut(s) 105, 280
HaeIII GGCC 1 cut(s) 279
HapII CCGG 1 cut(s) 155
HgaI GACGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 3 cut(s) 175, 209, 327
HpaII CCGG 1 cut(s) 155
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 206
Hpy188III TCNNGA 3 cut(s) 89, 244, 377
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 141
HpyCH4V TGCA 2 cut(s) 36, 318
Hsp92I GRCGYC 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 1 cut(s) 9
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 3 cut(s) 168, 210, 345
MaeI CTAG 1 cut(s) 182
MalI GATC 1 cut(s) 11
MboI GATC 1 cut(s) 9
MboII GAAGA 3 cut(s) 8, 11, 187
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 2 cut(s) 235, 349
MlyI GAGTC 2 cut(s) 169, 203
MnlI CCTC 8 cut(s) 132, 135, 138, 189, 203, 223, 251, 278
MspA1I CMGCKG 1 cut(s) 79
MspI CCGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 282
NdeII GATC 1 cut(s) 9
NlaIV GGNNCC 2 cut(s) 229, 255
PfeI GAWTC 1 cut(s) 327
PkrI GCNGC 2 cut(s) 106, 281
PleI GAGTC 2 cut(s) 169, 203
PpsI GAGTC 2 cut(s) 169, 203
PspN4I GGNNCC 2 cut(s) 229, 255
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SatI GCNGC 2 cut(s) 105, 280
Sau3AI GATC 1 cut(s) 9
SchI GAGTC 2 cut(s) 169, 203
SduI GDGCHC 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 2 cut(s) 235, 349
SsiI CCGC 4 cut(s) 51, 77, 105, 280
SspMI CTAG 1 cut(s) 182
TaqI TCGA 2 cut(s) 99, 336
TasI AATT 2 cut(s) 235, 349
TatI WGTACW 1 cut(s) 81
TauI GCSGC 2 cut(s) 107, 282
TfiI GAWTC 1 cut(s) 327
TspGWI ACGGA 1 cut(s) 205
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.