Rmu_sc0000546.1_g000064

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000546.1
OG0018007
Physical Location & Seq
Reverse (-)
303320 .. 303649
330 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
atggctattggtgactttaattccattttgggtgcacatgattttataggtgggcaggttcctaatcagtcttcatgtgaggatttccacagtgccatggatctctataacttcactcacttagacacagcaggtgctttcttcacttggtccaaaactttgtttggtggccacatggaacgtgagttagatcgtactttatgtgatggtcaatggttggattcttggtctcacactagttgcatggctcttcctctggccatctttgatcataattgtctggtcttcacggcaggcagtgctccatccaatggtccaaaacctttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

109

Amino Acids

11.98

Weight (kDa)

4.79

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
Acc36I ACCTGC 2 cut(s) 46, 122
AccB7I CCANNNNNTGG 1 cut(s) 311
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 2 cut(s) 169, 258
AfaI GTAC 1 cut(s) 196
AfiI CCNNNNNNNGG 1 cut(s) 311
AhlI ACTAGT 1 cut(s) 236
Alw21I GWGCWC 2 cut(s) 37, 304
Alw26I GTCTC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 33
AlwI GGATC 1 cut(s) 108
AoxI GGCC 2 cut(s) 169, 258
ApaLI GTGCAC 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 150, 314
AsuHPI GGTGA 1 cut(s) 23
AvaII GGWCC 2 cut(s) 150, 314
BaeGI GKGCMC 1 cut(s) 37
BalI TGGCCA 2 cut(s) 171, 260
BbsI GAAGAC 2 cut(s) 63, 277
Bbv12I GWGCWC 2 cut(s) 37, 304
BccI CCATC 3 cut(s) 200, 269, 313
BceAI ACGGC 1 cut(s) 306
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 234
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 1 cut(s) 237
BfuAI ACCTGC 2 cut(s) 46, 122
Bme18I GGWCC 2 cut(s) 150, 314
BmgT120I GGNCC 2 cut(s) 150, 314
BmiI GGNNCC 1 cut(s) 60
BpiI GAAGAC 2 cut(s) 63, 277
BsaI GGTCTC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 37
BshFI GGCC 2 cut(s) 171, 260
BsiHKAI GWGCWC 2 cut(s) 37, 304
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 234
BsnI GGCC 2 cut(s) 171, 260
Bso31I GGTCTC 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 37, 304
Bsp143I GATC 3 cut(s) 100, 190, 268
Bsp19I CCATGG 1 cut(s) 96
BspANI GGCC 2 cut(s) 171, 260
BspLI GGNNCC 1 cut(s) 60
BspMI ACCTGC 2 cut(s) 46, 122
BspPI GGATC 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 255
BspTNI GGTCTC 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 3 cut(s) 100, 190, 268
BssT1I CCWWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 1 cut(s) 255
BstAPI GCANNNNNTGC 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 1 cut(s) 121
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 305
BstKTI GATC 3 cut(s) 103, 193, 271
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 3 cut(s) 100, 190, 268
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstSLI GKGCMC 1 cut(s) 37
BstV2I GAAGAC 2 cut(s) 63, 277
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 171, 260
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 305
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 2 cut(s) 97, 304
BveI ACCTGC 2 cut(s) 46, 122
Cac8I GCNNGC 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 150, 314
Csp6I GTAC 1 cut(s) 195
CviAII CATG 5 cut(s) 38, 75, 97, 175, 244
CviJI RGCY 4 cut(s) 5, 171, 248, 260
CviKI_1 RGCY 4 cut(s) 5, 171, 248, 260
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 1 cut(s) 121
DpnI GATC 3 cut(s) 102, 192, 270
DpnII GATC 3 cut(s) 100, 190, 268
EaeI YGGCCR 2 cut(s) 169, 258
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco130I CCWWGG 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 234
Eco47I GGWCC 2 cut(s) 150, 314
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 5 cut(s) 41, 78, 100, 178, 247
FaiI YATR 9 cut(s) 39, 47, 76, 98, 108, 176, 202, 245, 273
FatI CATG 5 cut(s) 37, 74, 96, 174, 243
FbaI TGATCA 1 cut(s) 268
FokI GGATG 1 cut(s) 292
FspBI CTAG 1 cut(s) 237
HaeIII GGCC 2 cut(s) 171, 260
Hin1II CATG 5 cut(s) 41, 78, 100, 178, 247
HinfI GANTC 1 cut(s) 221
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 1 cut(s) 35
Hpy8I GTNNAC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 35, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 181
Hsp92II CATG 5 cut(s) 41, 78, 100, 178, 247
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 100, 190, 268
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 307
LpnPI CCDG 5 cut(s) 41, 117, 242, 266, 279
MaeI CTAG 1 cut(s) 237
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 1 cut(s) 11
MalI GATC 3 cut(s) 102, 192, 270
MboI GATC 3 cut(s) 100, 190, 268
MboII GAAGA 4 cut(s) 63, 133, 242, 277
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 37, 304
MlsI TGGCCA 2 cut(s) 171, 260
MluCI AATT 2 cut(s) 19, 274
MluNI TGGCCA 2 cut(s) 171, 260
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 2 cut(s) 73, 264
Mox20I TGGCCA 2 cut(s) 171, 260
MscI TGGCCA 2 cut(s) 171, 260
MseI TTAA 1 cut(s) 18
Msp20I TGGCCA 2 cut(s) 171, 260
MwoI GCNNNNNNNGC 1 cut(s) 299
NcoI CCATGG 1 cut(s) 96
NdeII GATC 3 cut(s) 100, 190, 268
NlaIII CATG 5 cut(s) 41, 78, 100, 178, 247
NlaIV GGNNCC 1 cut(s) 60
NmuCI GTSAC 1 cut(s) 11
PaqCI CACCTGC 1 cut(s) 122
PciSI GCTCTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 311
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 2 cut(s) 150, 314
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 18
Sau3AI GATC 3 cut(s) 100, 190, 268
Sau96I GGNCC 2 cut(s) 150, 314
SduI GDGCHC 2 cut(s) 37, 304
SetI ASST 5 cut(s) 52, 60, 136, 184, 325
SinI GGWCC 2 cut(s) 150, 314
SpeI ACTAGT 1 cut(s) 236
Sse9I AATT 2 cut(s) 19, 274
SspMI CTAG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 1 cut(s) 184
TasI AATT 2 cut(s) 19, 274
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 97, 304
TseFI GTSAC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 63
TspRI CASTG 2 cut(s) 97, 304
Van91I CCANNNNNTGG 1 cut(s) 311
VneI GTGCAC 1 cut(s) 33
VpaK11BI GGWCC 2 cut(s) 150, 314
XspI CTAG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.